Peptide microarrays for detailed, high-throughput
... reagent consumption were reduced considerably. PKA was shown to phosphorylate many peptides containing known PKA phosphorylation sites as well as some new substrates. Data in table 1 shows that staurosporine is a full ATP competitive inhibitor whereas AMP-PNP has a different inhibition mechanism. ...
... reagent consumption were reduced considerably. PKA was shown to phosphorylate many peptides containing known PKA phosphorylation sites as well as some new substrates. Data in table 1 shows that staurosporine is a full ATP competitive inhibitor whereas AMP-PNP has a different inhibition mechanism. ...
Protein dynamics and proteolysis in plant vacuoles
... cleaved from the precursor and it enters the biosynthetic pathway (also called the secretion pathway). Large segments of the precursor correspond to the mature vacuolar protein. Precursors can contain more than one subunit of the mature protein (e.g. 2S and 11S storage proteins; Müntz, 1998) or eve ...
... cleaved from the precursor and it enters the biosynthetic pathway (also called the secretion pathway). Large segments of the precursor correspond to the mature vacuolar protein. Precursors can contain more than one subunit of the mature protein (e.g. 2S and 11S storage proteins; Müntz, 1998) or eve ...
Enzymes - CEA Workshop Teacher Notes.pptx
... • A coenzyme is a ‘substance’ (usually an organic molecule) required to assist enzyme acJvity • A coenzyme will bind to a protein to form an acJve enzyme • Coenzymes osen help by carrying a group ...
... • A coenzyme is a ‘substance’ (usually an organic molecule) required to assist enzyme acJvity • A coenzyme will bind to a protein to form an acJve enzyme • Coenzymes osen help by carrying a group ...
EnzymesLect1 2014
... acids, which folds in a particular fashion to produce a three-dimensional product. Individual monomers may then combine via non-covalent interactions to form a multimeric protein. Many enzymes can be unfolded or inactivated by heating, which destroys the threedimensional structure of the protein. Ac ...
... acids, which folds in a particular fashion to produce a three-dimensional product. Individual monomers may then combine via non-covalent interactions to form a multimeric protein. Many enzymes can be unfolded or inactivated by heating, which destroys the threedimensional structure of the protein. Ac ...
ASM book 1.8.7.20 vgv - BioQUEST Curriculum Consortium
... distinct domains. Some bacteria-like organisms look like normal bacteria but may have had a distinct phylogenetic history. Consequently, these bacteria-like organisms may comprise a distinct domain, given the name Archaebacteria, or more simply, Archaea. The name reflects an untested conjecture abou ...
... distinct domains. Some bacteria-like organisms look like normal bacteria but may have had a distinct phylogenetic history. Consequently, these bacteria-like organisms may comprise a distinct domain, given the name Archaebacteria, or more simply, Archaea. The name reflects an untested conjecture abou ...
instructions on the annotation of pdf files
... boundaries that may differ in the two databases. We also required that each domain is formed by a continuous sequence of 50–300 residues and is, thus, in the range where ACO and chain length were shown to have predictive value. Finally, we only considered two-domain proteins in which the combined le ...
... boundaries that may differ in the two databases. We also required that each domain is formed by a continuous sequence of 50–300 residues and is, thus, in the range where ACO and chain length were shown to have predictive value. Finally, we only considered two-domain proteins in which the combined le ...
Effects of two kinds of imidazolium
... basis, and metabolic pathway of steroid-transforming A. simplex (ASP) cells. In this work, 1-dehydrogenation of the cortisone acetate, an important reaction in steroid medicine, was used as model reaction. A strategy in which ASP cells were pretreated with two ILs and washed for three times with KH2 ...
... basis, and metabolic pathway of steroid-transforming A. simplex (ASP) cells. In this work, 1-dehydrogenation of the cortisone acetate, an important reaction in steroid medicine, was used as model reaction. A strategy in which ASP cells were pretreated with two ILs and washed for three times with KH2 ...
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular
... current structure is computed dynamically as the molecular strand folds. Ball and stick view: It uses a ball representing each particle and sticks representing the chain segment along the backbone. Balls can be deformed into ellipsoids that show the current velocity of each particle. Isosurface view ...
... current structure is computed dynamically as the molecular strand folds. Ball and stick view: It uses a ball representing each particle and sticks representing the chain segment along the backbone. Balls can be deformed into ellipsoids that show the current velocity of each particle. Isosurface view ...
Insights into polypharmacology from drug
... same drug. More than half of the drugs in the drug–target network formed a giant interconnected cluster, and in both *To whom correspondence should be addressed. ...
... same drug. More than half of the drugs in the drug–target network formed a giant interconnected cluster, and in both *To whom correspondence should be addressed. ...
Lethal protein produced in response to competition
... it is suggested that high subtilisin levels will initially promote reproduction even on agar plates if the colony can expand fast enough. Indeed, when two colonies are competing, there is faster expansion at the facing fronts at the early stages of growth (SI Text), likely because of the added low l ...
... it is suggested that high subtilisin levels will initially promote reproduction even on agar plates if the colony can expand fast enough. Indeed, when two colonies are competing, there is faster expansion at the facing fronts at the early stages of growth (SI Text), likely because of the added low l ...
On-line tools for sequence retrieval and
... statistics for sequence analysis, it has to provide access to sequence data banks. For that purpose we have developed WWW-Query, a WWW version of the retrieval system Query (Gouy et al., 1985). As with its standalone predecessor, WWW-Query allows users to make complex queries on various sequence ban ...
... statistics for sequence analysis, it has to provide access to sequence data banks. For that purpose we have developed WWW-Query, a WWW version of the retrieval system Query (Gouy et al., 1985). As with its standalone predecessor, WWW-Query allows users to make complex queries on various sequence ban ...
The Structure and Function of Large Biological Molecules
... carbon skeleton, this representation is not completely accurate. In aqueous solutions, glucose molecules, as well as most other five- and six-carbon sugars, form rings (Figure 5.4). Monosaccharides, particularly glucose, are major nutrients for cells. In the process known as cellular respiration, cel ...
... carbon skeleton, this representation is not completely accurate. In aqueous solutions, glucose molecules, as well as most other five- and six-carbon sugars, form rings (Figure 5.4). Monosaccharides, particularly glucose, are major nutrients for cells. In the process known as cellular respiration, cel ...
Interplay of pH and Binding of Multivalent Metal Ions: Charge
... cause overcharging of the surface, attraction between likecharged surfaces, and charge inversion of colloidal particles.30−35 A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving ...
... cause overcharging of the surface, attraction between likecharged surfaces, and charge inversion of colloidal particles.30−35 A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving ...
Back-translation Using First Order Hidden Markov Models
... Spanish training set would give the most accurate back-translation of the three sets and the Russian set would give the least accurate. Surprisingly, our results did not mirror the > 70% accuracy that the Easyback group claimed and, at the very least, we had expected. Rather, each of training sets p ...
... Spanish training set would give the most accurate back-translation of the three sets and the Russian set would give the least accurate. Surprisingly, our results did not mirror the > 70% accuracy that the Easyback group claimed and, at the very least, we had expected. Rather, each of training sets p ...
Biology 13A Lab #13: Nutrition and Digestion
... then you can check to determine whether your results are accurate. We will then proceed to testing nutrient contents of a variety of foods. Procedure 1. Obtain 4 small plastic “medicine” cups. Use a wax pencil to label them from #1 through #4, for the four samples. 2. Pour approximately 5 mL of each ...
... then you can check to determine whether your results are accurate. We will then proceed to testing nutrient contents of a variety of foods. Procedure 1. Obtain 4 small plastic “medicine” cups. Use a wax pencil to label them from #1 through #4, for the four samples. 2. Pour approximately 5 mL of each ...
Exploring the Effects of Human Cytomegalovirus Infection on Host
... latently infect a majority of the world’s population. Though infections are usually minor, HCMV can cause serious problems in the immunocompromised and is a leading cause of birth defects. When HCMV infects a human cell, it effectively turns the cell into a “virus-producing factory”, taking control ...
... latently infect a majority of the world’s population. Though infections are usually minor, HCMV can cause serious problems in the immunocompromised and is a leading cause of birth defects. When HCMV infects a human cell, it effectively turns the cell into a “virus-producing factory”, taking control ...
03 Enzymes2
... - At a fixed enzyme concentration [E], the initial velocity Vo is almost linearly proportional to substrate concentration [S] when [S] is small but is nearly independent of [S] when [S] is large ...
... - At a fixed enzyme concentration [E], the initial velocity Vo is almost linearly proportional to substrate concentration [S] when [S] is small but is nearly independent of [S] when [S] is large ...
Separation and analysis of amino acids
... • Glycine is a precursor of porphyrins such as heme. • Arginine is a precursor of nitric oxide. • Carnitine, made in the body from the amino acids lysine and methionine, is used in lipid transport within a cell. • Ornithine and S-adenosylmethionine are precursors of polyamines. • Ornithine and Citru ...
... • Glycine is a precursor of porphyrins such as heme. • Arginine is a precursor of nitric oxide. • Carnitine, made in the body from the amino acids lysine and methionine, is used in lipid transport within a cell. • Ornithine and S-adenosylmethionine are precursors of polyamines. • Ornithine and Citru ...
Anti-Human Ciliary Neurotrophic Factor (CNTF) (C3960)
... factor for developing ciliary ganglionic neurons.2 ...
... factor for developing ciliary ganglionic neurons.2 ...
Bioinformatic approach to identify penultimate amino acids efficient
... NME is carried out by methionine aminopeptidase (MAP). Eubacteria contain only one type of MAP whereas eukaryotes contain two (MAP1 and MAP2). The efficiency of NME depends heavily on the penultimate (the second) amino acid. In the yeast, Saccharomyces cerevisiae, NME occurs most efficiently when th ...
... NME is carried out by methionine aminopeptidase (MAP). Eubacteria contain only one type of MAP whereas eukaryotes contain two (MAP1 and MAP2). The efficiency of NME depends heavily on the penultimate (the second) amino acid. In the yeast, Saccharomyces cerevisiae, NME occurs most efficiently when th ...
Lecture14Plants-L-type
... specificity show an explicit correlation. Monosaccharide specificity and number of gaps are indicated at the right. Key residues are highlighted in blue and highly conserved residues have been indicated with an asterisk. ...
... specificity show an explicit correlation. Monosaccharide specificity and number of gaps are indicated at the right. Key residues are highlighted in blue and highly conserved residues have been indicated with an asterisk. ...
Human GM-CSF ELISA
... has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conj ...
... has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conj ...
The Electron Transport Chain
... which transfers it to cytochrome c1. This process releases another 2 protons to the intermembrane space. The second electron is transferred to the bL heme to generate a second molecule of reoxidized CoQ. The bL heme transfers its electron to the bH heme. This electron is then transferred to the CoQx ...
... which transfers it to cytochrome c1. This process releases another 2 protons to the intermembrane space. The second electron is transferred to the bL heme to generate a second molecule of reoxidized CoQ. The bL heme transfers its electron to the bH heme. This electron is then transferred to the CoQx ...
Lipid raft–associated protein sorting in exosomes
... After removal of sucrose by ultracentrifugation in PBS, membrane fractions were loaded on formvar-carbon–coated grids for 30 minutes and immunogold labeled and constrasted as described. ...
... After removal of sucrose by ultracentrifugation in PBS, membrane fractions were loaded on formvar-carbon–coated grids for 30 minutes and immunogold labeled and constrasted as described. ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.