Eukaryotic-type protein kinases in Streptomyces
... more important role in prokaryotes, some groups of bacteria were recently shown to employ both systems. The first Ser/Thr protein kinase (ESTPK; stands for eukaryotictype Ser/Thr protein kinase) gene was isolated from Myxococcus xanthus by Munoz-Dorado et al. (1991). Since then, numerous genes have ...
... more important role in prokaryotes, some groups of bacteria were recently shown to employ both systems. The first Ser/Thr protein kinase (ESTPK; stands for eukaryotictype Ser/Thr protein kinase) gene was isolated from Myxococcus xanthus by Munoz-Dorado et al. (1991). Since then, numerous genes have ...
Document
... Wu FQ et al., The magnesium-chelatase H subunit binds abscisic acid and functions in abscisic acid signaling: new evidence in Arabidopsis. Plant Physiol. 2009 150:1940. Shang Y et al., The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-respo ...
... Wu FQ et al., The magnesium-chelatase H subunit binds abscisic acid and functions in abscisic acid signaling: new evidence in Arabidopsis. Plant Physiol. 2009 150:1940. Shang Y et al., The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-respo ...
Determination of protein regions responsible for interactions of
... and mouse LAMP1 by affinity pull-down and far-Western assays. In the present study, we used a Y2H assay to demonstrate the interaction of mouse full-length amelogenin M180 with human LAMP1 (Table 2, row e). Tetraspanin CD63 has two extracellular domains and three intracellular domains, with both the ...
... and mouse LAMP1 by affinity pull-down and far-Western assays. In the present study, we used a Y2H assay to demonstrate the interaction of mouse full-length amelogenin M180 with human LAMP1 (Table 2, row e). Tetraspanin CD63 has two extracellular domains and three intracellular domains, with both the ...
Amino acid analysis
... a. Place the protein sample in a hydrolysis tube, and dry. b. Add 200 μL of Hydrolysis Solution per 500 μg of protein. c. Freeze the sample tube in a dry ice-acetone bath, and flame seal in vacuum. d. Samples are typically hydrolyzed at 110ºC for 24 hours in vacuum or inert atmosphere to prevent oxi ...
... a. Place the protein sample in a hydrolysis tube, and dry. b. Add 200 μL of Hydrolysis Solution per 500 μg of protein. c. Freeze the sample tube in a dry ice-acetone bath, and flame seal in vacuum. d. Samples are typically hydrolyzed at 110ºC for 24 hours in vacuum or inert atmosphere to prevent oxi ...
A Guide to Finding - University of Utah
... always obvious which one is best suited for a particular problem or whether a better solution exists. As a result, biologists may still be under- or inefficiently utilizing the Internet. Thus, there is a need for a clear and concise schema for finding biological information on the Internet. The aim ...
... always obvious which one is best suited for a particular problem or whether a better solution exists. As a result, biologists may still be under- or inefficiently utilizing the Internet. Thus, there is a need for a clear and concise schema for finding biological information on the Internet. The aim ...
gfp_exercise_ver5
... To explore the structure of GFP and its primary sequence, we will use StarBiochem, a protein 3D‐viewer. To get to StarBiochem, please navigate to http://web.mit.edu/star/biochem. Click on the Start button to launch the application. Click Trust when a prompt appears asking you if you trust ...
... To explore the structure of GFP and its primary sequence, we will use StarBiochem, a protein 3D‐viewer. To get to StarBiochem, please navigate to http://web.mit.edu/star/biochem. Click on the Start button to launch the application. Click Trust when a prompt appears asking you if you trust ...
Why is asymmetric synthesis important?
... The helical structure of nature’s polymeric systems (DNA/RNA) is controlled by the chirality of the sugar-phosphate backbone made up of deoxyribose or ribose. The 3-D structure of proteins, enzymes and receptors is partly determined by the chirality of the amino-acids in the peptide chain. Importanc ...
... The helical structure of nature’s polymeric systems (DNA/RNA) is controlled by the chirality of the sugar-phosphate backbone made up of deoxyribose or ribose. The 3-D structure of proteins, enzymes and receptors is partly determined by the chirality of the amino-acids in the peptide chain. Importanc ...
ETC_2012 Quiz
... Presenting complaint: However, she started complaining of a burning sensation in her throat and mouth which was followed by nausea and vomiting, diaphoresis, agitation, and dyspnea. On Examination: The nurse noticed an almond-like smell in her breath. Lab investigations: An arterial blood gas reveal ...
... Presenting complaint: However, she started complaining of a burning sensation in her throat and mouth which was followed by nausea and vomiting, diaphoresis, agitation, and dyspnea. On Examination: The nurse noticed an almond-like smell in her breath. Lab investigations: An arterial blood gas reveal ...
1. An inner engine keeps us alive
... For an engine to run, it needs fuel. To live, we also need fuel. In the animal kingdom, fuel is provided by the breakdown of foods. Hence, in order to stay alive, grow and perform many necessary functions, we, the humans, require constant supply of foodstuff. The breakdown of food and conversion to ...
... For an engine to run, it needs fuel. To live, we also need fuel. In the animal kingdom, fuel is provided by the breakdown of foods. Hence, in order to stay alive, grow and perform many necessary functions, we, the humans, require constant supply of foodstuff. The breakdown of food and conversion to ...
Lecture 4
... despite their polar sulfur atoms, tend to be buried in the interior of proteins, similarly to hydrophobic residues. Serine and threonine are polar because of their –OH (hydroxyl) terminal groups; and asparagine and glutamine are polar because of their (C=O)-NH2 groups at the sidechain termini. These ...
... despite their polar sulfur atoms, tend to be buried in the interior of proteins, similarly to hydrophobic residues. Serine and threonine are polar because of their –OH (hydroxyl) terminal groups; and asparagine and glutamine are polar because of their (C=O)-NH2 groups at the sidechain termini. These ...
Dog and human acid ,-D-galactosidases are structurally similar
... against homogeneous human liver f-galactosidase (A2 plus A3) (Frost et al., 1978) was used to determine cross-reactivity of the dog, cat and human enzymes. Serial dilutions of goat anti-(human f-galactosidase) serum were performed in 10mMphosphate buffer, pH 7.0, containing 0.1 M-NaCl plus 0.02% NaN ...
... against homogeneous human liver f-galactosidase (A2 plus A3) (Frost et al., 1978) was used to determine cross-reactivity of the dog, cat and human enzymes. Serial dilutions of goat anti-(human f-galactosidase) serum were performed in 10mMphosphate buffer, pH 7.0, containing 0.1 M-NaCl plus 0.02% NaN ...
Chocolate Milk Aids in Post-Exercise Recovery The next time your
... protein is more beneficial to athletes than just carbohydrate alone in the post-workout meal or supplement. This study takes it one step further by identifying a food product - chocolate milk that is easy to get, inexpensive, and tastes great. My only suggestion is that you reach for a non-fat or sk ...
... protein is more beneficial to athletes than just carbohydrate alone in the post-workout meal or supplement. This study takes it one step further by identifying a food product - chocolate milk that is easy to get, inexpensive, and tastes great. My only suggestion is that you reach for a non-fat or sk ...
Chocolate Milk Aids in Post-Exercise Recovery The next
... protein is more beneficial to athletes than just carbohydrate alone in the post-workout meal or supplement. This study takes it one step further by identifying a food product - chocolate milk that is easy to get, inexpensive, and tastes great. My only suggestion is that you reach for a non-fat or sk ...
... protein is more beneficial to athletes than just carbohydrate alone in the post-workout meal or supplement. This study takes it one step further by identifying a food product - chocolate milk that is easy to get, inexpensive, and tastes great. My only suggestion is that you reach for a non-fat or sk ...
the simple quad method - New Moon Family Acupuncture
... A simple way of eating is called The Simple Quad Method of Food Apportionment™ (or Simple Quad™ for short). Eating the Simple Quad™ way means that each meal consists of ¼ Raw Veggies, ¼ Cooked Veggies, ¼ Protein, ¼ Carbohydrate, and a small amount of Fat. Your serving size for the entire meal should ...
... A simple way of eating is called The Simple Quad Method of Food Apportionment™ (or Simple Quad™ for short). Eating the Simple Quad™ way means that each meal consists of ¼ Raw Veggies, ¼ Cooked Veggies, ¼ Protein, ¼ Carbohydrate, and a small amount of Fat. Your serving size for the entire meal should ...
(9E10): sc-40 - Santa Cruz Biotechnology
... c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. The presence of three sequence motifs in the c-Myc COOH termi ...
... c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. The presence of three sequence motifs in the c-Myc COOH termi ...
View document as PDF
... proteins, amino acids, through the different levels of protein structure. Using the MolyMod© models, students learn the different atomic components of an amino acid and how a peptide bond is formed through the loss of a water molecule. The Water Cup provides an overview of how water is essential for ...
... proteins, amino acids, through the different levels of protein structure. Using the MolyMod© models, students learn the different atomic components of an amino acid and how a peptide bond is formed through the loss of a water molecule. The Water Cup provides an overview of how water is essential for ...
Ch 9 and 11 Review Slides
... protein (pink) that brings about the cell’s response to the signal. ...
... protein (pink) that brings about the cell’s response to the signal. ...
chapt06b_lecture
... Eukaryotic cells are divided into organelles which often allows for separation of opposing processes. Eg. Fatty acid oxidation occurs in the mitochondria while synthesis occurs in the cytosol. Organelles also allow for concentration of specific reagents. Eg. Lysosomes require a low pH (~5) and their ...
... Eukaryotic cells are divided into organelles which often allows for separation of opposing processes. Eg. Fatty acid oxidation occurs in the mitochondria while synthesis occurs in the cytosol. Organelles also allow for concentration of specific reagents. Eg. Lysosomes require a low pH (~5) and their ...
Replication-dependent fitness recovery of Human immunodeficiency
... peptide with Gly at the same position. Additional experiments need to be performed to resolve this discrepancy. Although the altered proteolytic processing of NC at the studied site is a feasible explanation for the altered infectivity, other explanations may also be valid. Detailed virological stud ...
... peptide with Gly at the same position. Additional experiments need to be performed to resolve this discrepancy. Although the altered proteolytic processing of NC at the studied site is a feasible explanation for the altered infectivity, other explanations may also be valid. Detailed virological stud ...
Fulltext PDF - Indian Academy of Sciences
... While chemistry provides the framework for understanding the structure and function of biomolecules, the immune system provides a highly evolved natural process to generate one class of complex biomolecules – the antibodies. A combination of the two could be exploited to generate new classes of mole ...
... While chemistry provides the framework for understanding the structure and function of biomolecules, the immune system provides a highly evolved natural process to generate one class of complex biomolecules – the antibodies. A combination of the two could be exploited to generate new classes of mole ...
Rapid purification of heart muscle enzymes using dye affinity
... while aqueous two-phase systems present a sterically unhindered ligand molecule to the protein's binding site. Although the aqueous two-phase systems, in the present work, were performed using small volumes systems, the results can be extrapolated to large volume preparations. The scaling up would i ...
... while aqueous two-phase systems present a sterically unhindered ligand molecule to the protein's binding site. Although the aqueous two-phase systems, in the present work, were performed using small volumes systems, the results can be extrapolated to large volume preparations. The scaling up would i ...
A Simple Method for Displaying the Hydropathic Character of a Protein
... The computer program, SOAP, assigns the appropriate hydropathy value to each residue in a given amino acid sequence and then successively sums those values, starting at the ammo terminal, within overlapping segments displaced from each other by one residue. Although a segment of any size can be chos ...
... The computer program, SOAP, assigns the appropriate hydropathy value to each residue in a given amino acid sequence and then successively sums those values, starting at the ammo terminal, within overlapping segments displaced from each other by one residue. Although a segment of any size can be chos ...
PSLDoc: Protein subcellular localization prediction based on
... sequence can be considered as the content of a document, and localization sites are considered as categories. To predict the localization site(s) of a protein is equivalent to predicting the category (e.g., sport, politics) of a document (e.g., a piece of news). This transformation is intuitive. Doc ...
... sequence can be considered as the content of a document, and localization sites are considered as categories. To predict the localization site(s) of a protein is equivalent to predicting the category (e.g., sport, politics) of a document (e.g., a piece of news). This transformation is intuitive. Doc ...
Printer Friendly PDF
... conditions, such as the type of alcohol (ethanol vs. isopropanol), the concentration of alcohol (typically 5095% v/v ethanol or isopropanol in water), temperature, reagents added (such as alkali or reducing agents) and the raw material (native corn kernels or corn processing by-products), as well as ...
... conditions, such as the type of alcohol (ethanol vs. isopropanol), the concentration of alcohol (typically 5095% v/v ethanol or isopropanol in water), temperature, reagents added (such as alkali or reducing agents) and the raw material (native corn kernels or corn processing by-products), as well as ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.