Nucleotide sequence of the 3h-terminal two
... the leafroll virus disease. The NY1 isolate of GLRaV-3 used in this study was first characterized in our laboratory by Zee et al. (1987). We demonstrated that closterovirus-like particles were closely associated with the disease and BYV-type vesicles were observed in leafroll-infected tissues (Zee e ...
... the leafroll virus disease. The NY1 isolate of GLRaV-3 used in this study was first characterized in our laboratory by Zee et al. (1987). We demonstrated that closterovirus-like particles were closely associated with the disease and BYV-type vesicles were observed in leafroll-infected tissues (Zee e ...
Nucleotide sequence of the 3h-terminal two
... the leafroll virus disease. The NY1 isolate of GLRaV-3 used in this study was first characterized in our laboratory by Zee et al. (1987). We demonstrated that closterovirus-like particles were closely associated with the disease and BYV-type vesicles were observed in leafroll-infected tissues (Zee e ...
... the leafroll virus disease. The NY1 isolate of GLRaV-3 used in this study was first characterized in our laboratory by Zee et al. (1987). We demonstrated that closterovirus-like particles were closely associated with the disease and BYV-type vesicles were observed in leafroll-infected tissues (Zee e ...
49. enzyme review - Khan Usman Ghani
... substrate as a result substrate is converted to product. Substrate binds on active site of enzymes that is specific for substrate (Hansen et al., 1990). Enzymes increases or decreases rate of reaction by increasing or decreasing the energy of activation (Amyes et al., 2001). Protein part of enzymes ...
... substrate as a result substrate is converted to product. Substrate binds on active site of enzymes that is specific for substrate (Hansen et al., 1990). Enzymes increases or decreases rate of reaction by increasing or decreasing the energy of activation (Amyes et al., 2001). Protein part of enzymes ...
Slide 1
... hnRNP and SR proteins... Multimerization of hnRNP A1 from a high affinity ESS can be sterically blocked by interaction of SF2/ASF with ESE”. Show predicted results of gel mobility shift experiments. ...
... hnRNP and SR proteins... Multimerization of hnRNP A1 from a high affinity ESS can be sterically blocked by interaction of SF2/ASF with ESE”. Show predicted results of gel mobility shift experiments. ...
Gene Expression Profiling During Asexual Development of the Late
... A second assessment of the GeneChips measured the frequency of false changes between technical replicates. In all possible pair-wise comparisons between five GeneChips probed with RNA from the same hyphal sample, on average, 0.07% of genes changed by more than twofold; such genes all exhibited expre ...
... A second assessment of the GeneChips measured the frequency of false changes between technical replicates. In all possible pair-wise comparisons between five GeneChips probed with RNA from the same hyphal sample, on average, 0.07% of genes changed by more than twofold; such genes all exhibited expre ...
Adv. Protein Chem. Struct. Biol.
... Extreme cases of protein flexibility are the molten globule state and the intrinsically disordered proteins. These exhibit significant conformational flexibility which is frequently necessary for the establishment of intermolecular interaction networks (Ohgushi and Wada, 1983; Gazi et al., 2009; Sch ...
... Extreme cases of protein flexibility are the molten globule state and the intrinsically disordered proteins. These exhibit significant conformational flexibility which is frequently necessary for the establishment of intermolecular interaction networks (Ohgushi and Wada, 1983; Gazi et al., 2009; Sch ...
The Amino-terminal Domain of the Golgi Protein Giantin Interacts
... SDS-PAGE and Western Blotting—Proteins were solubilized in SDSPAGE sample buffer, boiled for 4 min, and analyzed on 6, 10, or 12% SDS-polyacrylamide gels (51, 52). For Western blotting, proteins were transferred onto Hybond C blots (Amersham Pharmacia Biotech, Uppsala, Sweden) using a semidry blotte ...
... SDS-PAGE and Western Blotting—Proteins were solubilized in SDSPAGE sample buffer, boiled for 4 min, and analyzed on 6, 10, or 12% SDS-polyacrylamide gels (51, 52). For Western blotting, proteins were transferred onto Hybond C blots (Amersham Pharmacia Biotech, Uppsala, Sweden) using a semidry blotte ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... The purpose of using quantitative pattern matching was to possibly identify known motifs common to phosphorylated proteins. Perhaps documented motifs existed, initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases r ...
... The purpose of using quantitative pattern matching was to possibly identify known motifs common to phosphorylated proteins. Perhaps documented motifs existed, initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases r ...
Prokaryotic orthologues of mitochondrial alternative oxidase and plastid terminal oxidase
... Josse et al., 2003). The IMMUTANS mutation indicates that PTOX is required for carotenoid biosynthesis (Carol et al., 1999; Wu et al., 1999) and, more generally, it may represent the elusive terminal oxidase in chlororespiration (Peltier and Cournac, 2002). PTOX has been identified in plants and som ...
... Josse et al., 2003). The IMMUTANS mutation indicates that PTOX is required for carotenoid biosynthesis (Carol et al., 1999; Wu et al., 1999) and, more generally, it may represent the elusive terminal oxidase in chlororespiration (Peltier and Cournac, 2002). PTOX has been identified in plants and som ...
Primary production of protein: I. Comparison of net cellular carbon
... Specifically, there has not yet been a systematic evaluation of conditions required for the precise and accurate quantitation of protein synthesis by marine phytoplankton based on the incorporation of '"C-carbon. Radioisotopic tracers other than "C associated with distinct types of algal cell materi ...
... Specifically, there has not yet been a systematic evaluation of conditions required for the precise and accurate quantitation of protein synthesis by marine phytoplankton based on the incorporation of '"C-carbon. Radioisotopic tracers other than "C associated with distinct types of algal cell materi ...
Comparative Modeling of Mainly
... NCBI’s protein sequence database: • Many proteins of similar function to the known betahelices; some with similar sequences. • A significant fraction are characterized as microbial ...
... NCBI’s protein sequence database: • Many proteins of similar function to the known betahelices; some with similar sequences. • A significant fraction are characterized as microbial ...
Plant Protease Inhibitors - ReadingSample - Beck-Shop
... serine proteinases, interacting with the active sites in a ‘canonical’, i.e. substrate-like manner via an exposed reactive site loop of conserved conformation. More recently, some noncanonically binding serine proteinase inhibitors directed against coagulation factors, in particular thrombin, a few ...
... serine proteinases, interacting with the active sites in a ‘canonical’, i.e. substrate-like manner via an exposed reactive site loop of conserved conformation. More recently, some noncanonically binding serine proteinase inhibitors directed against coagulation factors, in particular thrombin, a few ...
SPA Enzyme Assay Design
... The source of enzyme for activity measurements in SPA assays can be obtained from either cell lysates (crude protein) or from purified preparations (e.g., recombinant protein). In the case of crude enzyme, competing activities may also be present in the cell lysate that could affect the assay perfor ...
... The source of enzyme for activity measurements in SPA assays can be obtained from either cell lysates (crude protein) or from purified preparations (e.g., recombinant protein). In the case of crude enzyme, competing activities may also be present in the cell lysate that could affect the assay perfor ...
Translocation of proteins across the cell envelope of Gram
... conservation, suggesting similar functions and working mechanisms. Despite this fact, several differences can be identified such as the absence of a clear homolog of the secretion-specific chaperone SecB in Gram-positive bacteria. The now available detailed insight into the organization of the Gram- ...
... conservation, suggesting similar functions and working mechanisms. Despite this fact, several differences can be identified such as the absence of a clear homolog of the secretion-specific chaperone SecB in Gram-positive bacteria. The now available detailed insight into the organization of the Gram- ...
Horse and Cattle Digestion
... This workforce solution was funded by a grant awarded under the Workforce Innovation in Regional Development (WIRED) as implemented by the U.S. Department of Labor’s Employment and Training Administration working in partnership with the Colorado Department of Labor and Employment, the Metro Denver ...
... This workforce solution was funded by a grant awarded under the Workforce Innovation in Regional Development (WIRED) as implemented by the U.S. Department of Labor’s Employment and Training Administration working in partnership with the Colorado Department of Labor and Employment, the Metro Denver ...
Telomerase Is a True Reverse Transcriptase
... shown in Fig. 1, these occur in the carboxy terminal half of each protein. Telomerase has for many years been called a "specialized reverse transcriptase" because it synthesizes DNA on an RNA template. The implication of the reverse transcriptase sequence motifs was that the protein structure respon ...
... shown in Fig. 1, these occur in the carboxy terminal half of each protein. Telomerase has for many years been called a "specialized reverse transcriptase" because it synthesizes DNA on an RNA template. The implication of the reverse transcriptase sequence motifs was that the protein structure respon ...
¹⁵N Heteronuclear Single Quantum Coherence
... Myoglobin has a rich scientific history. It has been of scientific interest since the early nineteenth century (Günther, 1921: Millikan, 1939), when the red pigment of mammalian muscle had been noted to be distinctive from that of the circulating blood. Work had been undertaken to isolate the pigmen ...
... Myoglobin has a rich scientific history. It has been of scientific interest since the early nineteenth century (Günther, 1921: Millikan, 1939), when the red pigment of mammalian muscle had been noted to be distinctive from that of the circulating blood. Work had been undertaken to isolate the pigmen ...
An LL-Diaminopimelate Aminotransferase
... have been discovered and the one that shows the widest taxonomic distribution uses N-succinylated intermediates (Gilvarg, 1959, 1961; Velasco et al., 2002). THDPA is succinylated by a succinylCoA-dependent transferase (DapD) that results in opening of the ring and exposure of a keto group that serve ...
... have been discovered and the one that shows the widest taxonomic distribution uses N-succinylated intermediates (Gilvarg, 1959, 1961; Velasco et al., 2002). THDPA is succinylated by a succinylCoA-dependent transferase (DapD) that results in opening of the ring and exposure of a keto group that serve ...
Cellular respiration - Jocha
... • Covalent bonds in the glucose and subsequent by-products are broken down and e- (electrons) from H (hydrogen) atoms are used in etransport to produce ATP ...
... • Covalent bonds in the glucose and subsequent by-products are broken down and e- (electrons) from H (hydrogen) atoms are used in etransport to produce ATP ...
Entropy in Biology -R-ES-O-N-A-N-C-E--I-s-e-p-te-m-b-e-r--2-0-0
... protein unfolding reaction can exist in a larger number of equivalent states, compared with the native state, entropy increases during the unfolding reaction. From the simple formula defined by Ludwig Boltzmann in 1877, the entropy of the unfolded state, which is equal to kIn W (where k is the Boltz ...
... protein unfolding reaction can exist in a larger number of equivalent states, compared with the native state, entropy increases during the unfolding reaction. From the simple formula defined by Ludwig Boltzmann in 1877, the entropy of the unfolded state, which is equal to kIn W (where k is the Boltz ...
Unzipping Genes - HiMedia Laboratories
... Precautions to be taken while handling RNA Ribonucleases (RNases) are very stable and active enzymes that generally do not require cofactors to function. Since RNases are difficult to inactivate and even trace amounts are sufficient to destroy RNA, do not use any plasticware or glassware without fir ...
... Precautions to be taken while handling RNA Ribonucleases (RNases) are very stable and active enzymes that generally do not require cofactors to function. Since RNases are difficult to inactivate and even trace amounts are sufficient to destroy RNA, do not use any plasticware or glassware without fir ...
spin-system assignments
... using 2-dimensional experiments to establish correlations between different 1H resonances. Recognition of characteristic patterns and networks of correlations then allows assignments to be made. Resonances are correlated either “through-bond”, mediated by the scalar coupling, or “through-space”, med ...
... using 2-dimensional experiments to establish correlations between different 1H resonances. Recognition of characteristic patterns and networks of correlations then allows assignments to be made. Resonances are correlated either “through-bond”, mediated by the scalar coupling, or “through-space”, med ...
Bioinformatics Tools Review ()
... the sequence above. Click on the link gi|1498054|gb|U64437.1|ZMU64437 (Zea mays novel protein mRNA, complete cds) to see the nucleotide sequence file. The sequence file is in Genbank format. Note that the accession number is U64437 and this sequence is an mRNA sequence. The nucleotide sequence U644 ...
... the sequence above. Click on the link gi|1498054|gb|U64437.1|ZMU64437 (Zea mays novel protein mRNA, complete cds) to see the nucleotide sequence file. The sequence file is in Genbank format. Note that the accession number is U64437 and this sequence is an mRNA sequence. The nucleotide sequence U644 ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.