Effect of Combining Bcl-2 Inhibition and Radiation on Apoptotic Cell
... when a cell has aged (senescent), and/or after potentially harmful mutations have damaged the cell, among other reasons. The morphological changes that occur in the nucleus during this process include chromatin condensation, nuclear fragmentation and DNA laddering. In the cytoplasm, apoptotic bodies ...
... when a cell has aged (senescent), and/or after potentially harmful mutations have damaged the cell, among other reasons. The morphological changes that occur in the nucleus during this process include chromatin condensation, nuclear fragmentation and DNA laddering. In the cytoplasm, apoptotic bodies ...
Multimeric Protein Structures of African Horsesickness Virus
... pFastbac-Oual. (B) A schematic representation of a partial restriction map of the recombinant dual transfer vector, pFBd-S6.9-S7.9. (C) An agarose gel electrophoretic analysis of the dual recombinant plasmid, pFBd-S6.9S7.9. Figure 3.6: ...
... pFastbac-Oual. (B) A schematic representation of a partial restriction map of the recombinant dual transfer vector, pFBd-S6.9-S7.9. (C) An agarose gel electrophoretic analysis of the dual recombinant plasmid, pFBd-S6.9S7.9. Figure 3.6: ...
Comparison of sequence-based and structure
... 3.2a Poor correlation with high sequence identities: It could be noted from table 2 that, while the majority of the families have low average sequence identities, three families have high average %I but have poor correlation coefficient. These families are either from multi-domain or small protein cl ...
... 3.2a Poor correlation with high sequence identities: It could be noted from table 2 that, while the majority of the families have low average sequence identities, three families have high average %I but have poor correlation coefficient. These families are either from multi-domain or small protein cl ...
Electron Transport Oxidative Phosphorylation Control
... mitochondrion depends on the impermeability of the inner mitochondrial membrane An agent that interacts with the inner mitochondrial membrane and increases its permeability to H+ would allow dissipation of H+ gradient, thus uncoupling oxidative phosphorylation from electron transport Production of h ...
... mitochondrion depends on the impermeability of the inner mitochondrial membrane An agent that interacts with the inner mitochondrial membrane and increases its permeability to H+ would allow dissipation of H+ gradient, thus uncoupling oxidative phosphorylation from electron transport Production of h ...
Chapter 26
... • Translational error rate is very small (~ 10-4 per codon), because there are two check mechanisms. 1. Initial recognition: Codon-anticodon binding energy discriminates between cognate and noncognate tRNA. 2. Proof-reading: After GTP hydrolysis, EF-Ts·GDP and aa-tRNA are separated. The dissociation ...
... • Translational error rate is very small (~ 10-4 per codon), because there are two check mechanisms. 1. Initial recognition: Codon-anticodon binding energy discriminates between cognate and noncognate tRNA. 2. Proof-reading: After GTP hydrolysis, EF-Ts·GDP and aa-tRNA are separated. The dissociation ...
Poster for RCPSC mee.. - University of Alberta
... Ceramide is a sphingolipid second messenger produced in response to cellular stress via activation of sphingomyelinases. Agonists that cause cellular production of ceramide include cytokines (TNF, Fas), agents of environmental stress (heat, UV irradiation), and chemotherapeutic agents. The accumulat ...
... Ceramide is a sphingolipid second messenger produced in response to cellular stress via activation of sphingomyelinases. Agonists that cause cellular production of ceramide include cytokines (TNF, Fas), agents of environmental stress (heat, UV irradiation), and chemotherapeutic agents. The accumulat ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... Clones for Expressing Bacterial Proteins–-The GST-Hairy fusion proteins bHLH, Orange-WRPW, N30 (an N-terminal 30-amino acid fragment of Hairy), Basic (basic DNA-binding elements), and HLH have been described previously (5, 16). Dmp53 (obtained from M. Brodsky) is a BamHI/BglII fragment inserted in t ...
... Clones for Expressing Bacterial Proteins–-The GST-Hairy fusion proteins bHLH, Orange-WRPW, N30 (an N-terminal 30-amino acid fragment of Hairy), Basic (basic DNA-binding elements), and HLH have been described previously (5, 16). Dmp53 (obtained from M. Brodsky) is a BamHI/BglII fragment inserted in t ...
In Sweden, Mexico, the United States, and a num
... the “acceptability” of killing prisoners, fetal tissue research depends on the “acceptability” of killing helpless, innocent human beings in the first place. (Certainly there is nothing wrong with using tissue from ectopic pregnancies or miscarriages; but this debate concerns babies who are purposel ...
... the “acceptability” of killing prisoners, fetal tissue research depends on the “acceptability” of killing helpless, innocent human beings in the first place. (Certainly there is nothing wrong with using tissue from ectopic pregnancies or miscarriages; but this debate concerns babies who are purposel ...
Ribosomes: Cashing in on crystals
... and assigned to regions of 16S rRNA on the basis of available biochemical data. The seven proteins of known highresolution structure have been placed in the density, assisted by a variety of data from previous studies indicating the approximate locations of these proteins within the 30S subunit. Fiv ...
... and assigned to regions of 16S rRNA on the basis of available biochemical data. The seven proteins of known highresolution structure have been placed in the density, assisted by a variety of data from previous studies indicating the approximate locations of these proteins within the 30S subunit. Fiv ...
ELECTRON TRANSPORT CHAIN (student)
... ELECTRON-TRANSPORT-CHAIN • In this step, we will utilize the energy provided by the electron carriers NADH and FADH2 •Extremely EXERGONIC ∆G = -2870 kJ/Mol ...
... ELECTRON-TRANSPORT-CHAIN • In this step, we will utilize the energy provided by the electron carriers NADH and FADH2 •Extremely EXERGONIC ∆G = -2870 kJ/Mol ...
Involvement of the glycine-rich RNA
... aggressive abiotic stress factor) we evaluated the germination capacity of the seeds obtained from the wild-type Col0 and the two mutant lines taken into consideration in this study. The ½ Murashige&Skoog (1962) culture medium containing growth vitamins but not sugar (used as control) was supplement ...
... aggressive abiotic stress factor) we evaluated the germination capacity of the seeds obtained from the wild-type Col0 and the two mutant lines taken into consideration in this study. The ½ Murashige&Skoog (1962) culture medium containing growth vitamins but not sugar (used as control) was supplement ...
Evidence for the absence of amino acid isomerization in microwave
... Samples of UHT milk and reconstituted formulas were heated under conditions corresponding to those applied a t home for warming up or cooking food (600 W for 3 min), as well as a t a lower power (70 W) for a prolonged time (20min). The latter conditions were applied as an attempt to distinguish an i ...
... Samples of UHT milk and reconstituted formulas were heated under conditions corresponding to those applied a t home for warming up or cooking food (600 W for 3 min), as well as a t a lower power (70 W) for a prolonged time (20min). The latter conditions were applied as an attempt to distinguish an i ...
Role of Pro-297 in the catalytic mechanism of sheep liver... hydroxymethyltransferase
... cleavage of serine and the transamination of -aspartate respectively. This is probably achieved by the participation of different amino acid residues in substrate binding and catalysis. In addition, both the enzymes catalyse decarboxylation, racemization and transamination, for example, apart from ...
... cleavage of serine and the transamination of -aspartate respectively. This is probably achieved by the participation of different amino acid residues in substrate binding and catalysis. In addition, both the enzymes catalyse decarboxylation, racemization and transamination, for example, apart from ...
Relationship between Hot Spot Residues and Ligand Binding Hot
... clusters identify the consensus binding sites, termed “consensus sites” (CS), and the relative importance of these CSs can be ranked according to the number of bound probe clusters they contain.32 It has been extensively verified that such consensus sites reliably identify the experimentally determin ...
... clusters identify the consensus binding sites, termed “consensus sites” (CS), and the relative importance of these CSs can be ranked according to the number of bound probe clusters they contain.32 It has been extensively verified that such consensus sites reliably identify the experimentally determin ...
Electron microscopy in structural studies of Photosystem II
... PS II is a multisubunit pigment–protein complex embedded in the thylakoid membranes of higher plants, algae and cyanobacteria (Hansson and Wydrzynski 1990; Ghanotakis and Yocum 1990; Barber 1998). It performs a series of photochemical reactions, which result in the reduction of plastoquinone, the ox ...
... PS II is a multisubunit pigment–protein complex embedded in the thylakoid membranes of higher plants, algae and cyanobacteria (Hansson and Wydrzynski 1990; Ghanotakis and Yocum 1990; Barber 1998). It performs a series of photochemical reactions, which result in the reduction of plastoquinone, the ox ...
Microsecond Rotational Dynamics of Spin-Labeled Ca
... vesicles were prepared from the white (fast) skeletal muscle of New Zealand white rabbits according to the method of Fernandez et al. (1980). The SR was suspended in sucrose buffer (0.3 M sucrose and 20 mM MOPS, pH 7.0) at a concentration of 20-40 mg/mL protein, rapidly frozen, and stored in liquid ...
... vesicles were prepared from the white (fast) skeletal muscle of New Zealand white rabbits according to the method of Fernandez et al. (1980). The SR was suspended in sucrose buffer (0.3 M sucrose and 20 mM MOPS, pH 7.0) at a concentration of 20-40 mg/mL protein, rapidly frozen, and stored in liquid ...
... and penicillin G, 10 U ml-I. Supplement was clarified by sterile filtration (0.2 pm) and then added to the cooled sterile basal medium. Cultures were inoculated (1 : 1000) with an exponential phase culture and incubated for 22-24 h at 37 "C. This procedure produced a colour changing unit titre (Rodw ...
GuanHongLi (275
... Besides supplying amino acids and energy that are essential for growth and maintenance, food proteins can act as an important source of biologically active peptides with antihypertensive, opioid, immunomodulating, antioxidative, antimicrobial, antithrombotic, antiamnestic, hypocholesterolemic and ot ...
... Besides supplying amino acids and energy that are essential for growth and maintenance, food proteins can act as an important source of biologically active peptides with antihypertensive, opioid, immunomodulating, antioxidative, antimicrobial, antithrombotic, antiamnestic, hypocholesterolemic and ot ...
Slides
... Increases the rate of chemical reaction / biological process Remains unchanged Biomolecules that catalyze chemical reactions Usually proteins ...
... Increases the rate of chemical reaction / biological process Remains unchanged Biomolecules that catalyze chemical reactions Usually proteins ...
Gene Section JUND (proto-oncogene) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... JUND are viable with only mild defects in growth and spermatogenesis, whereas mice lacking JUN or JUNB die in embryo. ...
... JUND are viable with only mild defects in growth and spermatogenesis, whereas mice lacking JUN or JUNB die in embryo. ...
12 Molecular Visualization of an Enzyme, Acetylcholinesterase
... with different settings and commands. We find that Chimera is relatively user-friendly and forgiving. Students are encouraged to download this free software for their own use. Chimera may be used with at-home assignments based on the lecture portion of a biochemistry course as well. This is a good o ...
... with different settings and commands. We find that Chimera is relatively user-friendly and forgiving. Students are encouraged to download this free software for their own use. Chimera may be used with at-home assignments based on the lecture portion of a biochemistry course as well. This is a good o ...
bcaa power - ProAction
... abundance of BCAA (and leucine in particular) in the muscle fibers has a further, powerful anabolic effect. To see how BCAA take effect in regulating the processes of protein synthesis and degradation, we need to be aware that insulin, IGF-1 and GH influence these same processes too. The effect may ...
... abundance of BCAA (and leucine in particular) in the muscle fibers has a further, powerful anabolic effect. To see how BCAA take effect in regulating the processes of protein synthesis and degradation, we need to be aware that insulin, IGF-1 and GH influence these same processes too. The effect may ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
Analysis of the glycoside hydrolase family 8 catalytic core in
... The glycoside hydrolase family 8 (GH-8) consists of bifunctional cellulase-chitosanases many of which are produced by species of Bacillus. Chitosanolytic enzymes can be useful in producing low molecular weight chitooligosaccharides which have several applications. In addition, a bifunctional enzyme ...
... The glycoside hydrolase family 8 (GH-8) consists of bifunctional cellulase-chitosanases many of which are produced by species of Bacillus. Chitosanolytic enzymes can be useful in producing low molecular weight chitooligosaccharides which have several applications. In addition, a bifunctional enzyme ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.