full abstracts - LSU WordPress sites
... Clean surfaces generated by “reverse engineering” using 3-D printing with polymeric surface materials of micro-topological surfaces that misalign to diminish the surface area of human contact could inhibit microbial adherence/attachment on commonuse surfaces such as cellphone/computer touchscreens, ...
... Clean surfaces generated by “reverse engineering” using 3-D printing with polymeric surface materials of micro-topological surfaces that misalign to diminish the surface area of human contact could inhibit microbial adherence/attachment on commonuse surfaces such as cellphone/computer touchscreens, ...
The RAGNYA fold: a novel fold with multiple topological variants
... for a biochemically diverse set of interactions with nucleic acids. Some notable examples of such folds are the RNA recognition motif (RRM)-like fold, double c-beta barrel (and the related EI barrel), b-grasp, S5-like fold, HhH (helix-hairpin-helix) and HTH (helix-turn-helix) [for further details se ...
... for a biochemically diverse set of interactions with nucleic acids. Some notable examples of such folds are the RNA recognition motif (RRM)-like fold, double c-beta barrel (and the related EI barrel), b-grasp, S5-like fold, HhH (helix-hairpin-helix) and HTH (helix-turn-helix) [for further details se ...
A-level Human Biology Question Paper Unit 01 - The Body
... 2 (a) (ii) What is the function of the RNA molecules in this virus? ...
... 2 (a) (ii) What is the function of the RNA molecules in this virus? ...
The Cleavage and Polyadenylation Specificity Factor in Xenopus
... Nuclei were removed from oocytes under mineral oil as described in reference 7. For collagenase treatment, enucleated oocytes were incubated for several hours in Marc’s modified Ringer’s (MMR) containing 1 mg of bovine serum albumin (Sigma, St. Louis, Mo.) per ml to allow healing. Healthy cells were ...
... Nuclei were removed from oocytes under mineral oil as described in reference 7. For collagenase treatment, enucleated oocytes were incubated for several hours in Marc’s modified Ringer’s (MMR) containing 1 mg of bovine serum albumin (Sigma, St. Louis, Mo.) per ml to allow healing. Healthy cells were ...
Mitochondrial dysfunction in neurodevelopmental disorders
... NADH to reduced NADH of 750:1, and a lactate-to-pyruvate ratio of 6 (as in autism) indicates a ratio of oxidized NADH to reduced NADH of 1500:1. ...
... NADH to reduced NADH of 750:1, and a lactate-to-pyruvate ratio of 6 (as in autism) indicates a ratio of oxidized NADH to reduced NADH of 1500:1. ...
Intrinsically Disordered Protein - Center for Data Analytics and
... a set of related but structurally distinct molecules. However, due to the appropriation of induced fit for explaining domain movements upon binding, the authors suggested multi-induced fit as a way to explain their data. Oxygen binding by hemoglobin and catalysis by aspartate transcarbamoylase are b ...
... a set of related but structurally distinct molecules. However, due to the appropriation of induced fit for explaining domain movements upon binding, the authors suggested multi-induced fit as a way to explain their data. Oxygen binding by hemoglobin and catalysis by aspartate transcarbamoylase are b ...
An Investigation into the Minimum Requirements for
... 57. The k,,, values for the H57A/D102N and H57L/D102N variants a t pH 8.0 were slightly higher and at pH 10.1 were much higher than those for the analogous H57A and H57L enzymes (Figure 2b; Table I). This increase in k,, may support an inhibitory effect of Asp 102 on catalysis by the latter variants ...
... 57. The k,,, values for the H57A/D102N and H57L/D102N variants a t pH 8.0 were slightly higher and at pH 10.1 were much higher than those for the analogous H57A and H57L enzymes (Figure 2b; Table I). This increase in k,, may support an inhibitory effect of Asp 102 on catalysis by the latter variants ...
Microbiology Ch 3 p18-37 [4-20
... b. To drive the transport of molecules, cell uses energy from electrochemical gradient of protons, the proton motive force i. Gradient is generated by extrusion of protons from cell (resulting from oxidation of metabolic intermediates like NADH or ATP hydrolysis ii. Lactose is accumulated intracellu ...
... b. To drive the transport of molecules, cell uses energy from electrochemical gradient of protons, the proton motive force i. Gradient is generated by extrusion of protons from cell (resulting from oxidation of metabolic intermediates like NADH or ATP hydrolysis ii. Lactose is accumulated intracellu ...
No Slide Title
... who is interested in the structures of proteins, nucleic acids, polysaccharides, and other complex substances with higher molecular weights will come to rely upon a new structural chemistry, involving precise geometrical relationships among the atoms in the molecules and the rigorous application of ...
... who is interested in the structures of proteins, nucleic acids, polysaccharides, and other complex substances with higher molecular weights will come to rely upon a new structural chemistry, involving precise geometrical relationships among the atoms in the molecules and the rigorous application of ...
PDF - Walter Lab
... 2 demonstrate that the synthetic mRNA directs the synthesis of paF as a single protein band of the correct molecular weight (Figure 2, lane 3) when translated in a yeast cell free extract. The identity of the primary translation product as paF is confirmed by immunoprecipitation with a rabbit antibo ...
... 2 demonstrate that the synthetic mRNA directs the synthesis of paF as a single protein band of the correct molecular weight (Figure 2, lane 3) when translated in a yeast cell free extract. The identity of the primary translation product as paF is confirmed by immunoprecipitation with a rabbit antibo ...
Biology 115 Lab 10:Gene Technology
... the DNA that is complementary in sequence. The unbound probe is washed off. ...
... the DNA that is complementary in sequence. The unbound probe is washed off. ...
Autophosphorylation Activity of the Arabidopsis Ethylene Receptor
... min. Reactions were run on 8% SDS-PAGE and blotted to PVDF membrane (Hybond-P, Amersham Biosciences) using a three-solution semidry protein blotting protocol for 30 min at 16 V optimized to a lower pH to avoid loss of phosphoester linkages. Following is the blotting setup in brief, from anode to cat ...
... min. Reactions were run on 8% SDS-PAGE and blotted to PVDF membrane (Hybond-P, Amersham Biosciences) using a three-solution semidry protein blotting protocol for 30 min at 16 V optimized to a lower pH to avoid loss of phosphoester linkages. Following is the blotting setup in brief, from anode to cat ...
Gene Section ABL (v-abl Abelson murine leukemia viral oncogene homolog 1)
... Online version is available at: http://AtlasGeneticsOncology.org/Genes/ABL.html DOI: 10.4267/2042/32041 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 1997 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
... Online version is available at: http://AtlasGeneticsOncology.org/Genes/ABL.html DOI: 10.4267/2042/32041 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 1997 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
COURSE SYLLABUS CHM 521 Biochemistry I 3(3
... The pentose phosphate pathway Glycogen breakdown and synthesis Regulation of carbohydrate metabolism Hormones and second messengers Oxidation of pyruvate The citric acid cycle Anabolic TCA cycle and glyoxylate cycle Regulation of the citric acid cycle Di- and oligosaccharide metabolism Mitochondria ...
... The pentose phosphate pathway Glycogen breakdown and synthesis Regulation of carbohydrate metabolism Hormones and second messengers Oxidation of pyruvate The citric acid cycle Anabolic TCA cycle and glyoxylate cycle Regulation of the citric acid cycle Di- and oligosaccharide metabolism Mitochondria ...
Molecular Characterization of a Hamster Oviduct
... bp) as the screening probe. Phage plaques were transferred to Hybond N + filters (Amersham). These transferred plaques on the membranes were denatured with alkaline solution (0.5 M NaOH containing 1.5 M NaCl) for 2 min and neutralized with neutral solution (1 M Tris-HCI [pH 7.0] containing 1.5 M NaC ...
... bp) as the screening probe. Phage plaques were transferred to Hybond N + filters (Amersham). These transferred plaques on the membranes were denatured with alkaline solution (0.5 M NaOH containing 1.5 M NaCl) for 2 min and neutralized with neutral solution (1 M Tris-HCI [pH 7.0] containing 1.5 M NaC ...
Preparation of Lentiviral expression construct DNA
... Secondary HRP conjugated antibody Calculate the amount of HRP conjugated antibody needed to perform the desired experiments and make 1:1000 dilution with 1x Binding Buffer B (100 μl/well). TMB substrate solution Take appropriate volume of TMB Substrate (100 μl/well) and warm it up to room temperatur ...
... Secondary HRP conjugated antibody Calculate the amount of HRP conjugated antibody needed to perform the desired experiments and make 1:1000 dilution with 1x Binding Buffer B (100 μl/well). TMB substrate solution Take appropriate volume of TMB Substrate (100 μl/well) and warm it up to room temperatur ...
The molecular machinery of Keilin`s respiratory chain
... mammalian mitochondrial electron transfer chains is complex I. Electron transfer within complex I, in contrast with the simpler NADH dehydrogenases, is coupled to proton translocation across the membrane, greatly increasing the ultimate yield of ATP that can be produced. Bovine complex I is remarkab ...
... mammalian mitochondrial electron transfer chains is complex I. Electron transfer within complex I, in contrast with the simpler NADH dehydrogenases, is coupled to proton translocation across the membrane, greatly increasing the ultimate yield of ATP that can be produced. Bovine complex I is remarkab ...
Relationship Between the Occurrence of Cysteine in Proteins and
... Cysteine is unique among coded amino acids because it contains a reactive sulph-hydryl group. Therefore, two cysteine residues may form a cystine (disulfide link) between various parts of the same protein or between two separate polypeptide chains. The formation of a disulfide link is coupled with t ...
... Cysteine is unique among coded amino acids because it contains a reactive sulph-hydryl group. Therefore, two cysteine residues may form a cystine (disulfide link) between various parts of the same protein or between two separate polypeptide chains. The formation of a disulfide link is coupled with t ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.