Protein structure
... During the translation of a gene into a protein, the protein is formed by the sequential joining of amino acids end-to-end to form a long chain-like molecule (polymer). A polymer of amino acids is often referred to as a polypeptide. The genome is capable of coding for 20 different amino acids whose ...
... During the translation of a gene into a protein, the protein is formed by the sequential joining of amino acids end-to-end to form a long chain-like molecule (polymer). A polymer of amino acids is often referred to as a polypeptide. The genome is capable of coding for 20 different amino acids whose ...
COMPUTATIONAL PERSPECTIVE IN THE STRUCTURAL STABILITY OF ‘ALLALPHA’ PROTEINS: THE NH...Π INTERACTIONS
... The values of S obtained for all the amino acids were plotted in Fig. 3. The percentage ratio calculated shows that Arg make the maximum contribution to this N‐H...π interaction (52 interactions in a total of 160 interactions). It might be due to the fact that the side chain of arginin ...
... The values of S obtained for all the amino acids were plotted in Fig. 3. The percentage ratio calculated shows that Arg make the maximum contribution to this N‐H...π interaction (52 interactions in a total of 160 interactions). It might be due to the fact that the side chain of arginin ...
Name____________________________ Student number
... 2. Which of the following statements is true of enzyme catalysts? A) To be effective, they must be present at the same concentration as their substrate. B) They can increase the equilibrium constant for a given reaction by a thousand-fold or more. •C) They lower the activation energy for conversion ...
... 2. Which of the following statements is true of enzyme catalysts? A) To be effective, they must be present at the same concentration as their substrate. B) They can increase the equilibrium constant for a given reaction by a thousand-fold or more. •C) They lower the activation energy for conversion ...
Nucleotide Sequence of the Gene Coding for the
... the nitrogenase proteins: 1) Are the amino acid sequences of nitrogenase proteinsconserved to the same extent throughout the polypeptide chains, or is the conservation only limited to specific regions of the proteins? 2) If specific regions are stronglyconserved, are these conservedregionsassociated ...
... the nitrogenase proteins: 1) Are the amino acid sequences of nitrogenase proteinsconserved to the same extent throughout the polypeptide chains, or is the conservation only limited to specific regions of the proteins? 2) If specific regions are stronglyconserved, are these conservedregionsassociated ...
lec39_2013 - Andrew.cmu.edu
... c) rRNA – ribosomal RNA is found in the ribosome and is responsible for most of the function in protein synthesis. ...
... c) rRNA – ribosomal RNA is found in the ribosome and is responsible for most of the function in protein synthesis. ...
SnRK1 Isoforms AKIN10 and AKIN11 Are
... Having established that no changes in gene expression were observed in the catalytic subunits during Pi starvation to explain the reduction in activity, we analyzed the protein levels. Transgenic Arabidopsis plants carrying AKIN10-GFP and AKIN11-GFP fusion proteins were evaluated. Localization of GF ...
... Having established that no changes in gene expression were observed in the catalytic subunits during Pi starvation to explain the reduction in activity, we analyzed the protein levels. Transgenic Arabidopsis plants carrying AKIN10-GFP and AKIN11-GFP fusion proteins were evaluated. Localization of GF ...
The Activity of a Developmentally Regulated Cysteine Proteinase Is
... gelatin-containing gels. The active fractions were pooled, concentrated, dissolved in 25 mM Tris-HCl, pH 8.0, and then applied to a Sephadex G-100 column. Finally, affinity chromatography using cystatin-Sepharose was performed as reported (29). Protein Microsequencing—Purified enzyme was transferred ...
... gelatin-containing gels. The active fractions were pooled, concentrated, dissolved in 25 mM Tris-HCl, pH 8.0, and then applied to a Sephadex G-100 column. Finally, affinity chromatography using cystatin-Sepharose was performed as reported (29). Protein Microsequencing—Purified enzyme was transferred ...
translation and protein structure
... charges in the interacting molecules, which are then attracted to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is als ...
... charges in the interacting molecules, which are then attracted to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is als ...
Cell and Organelle Movement
... d. The thick filaments of myosin are positioned uniformly between the Z line by a long template protein known as titin. 1. Titin acts a molecular spring and contains a long series of immunoglobulin like domains. D. Contraction accomplished by the sliding of myosin filaments passed the actin filament ...
... d. The thick filaments of myosin are positioned uniformly between the Z line by a long template protein known as titin. 1. Titin acts a molecular spring and contains a long series of immunoglobulin like domains. D. Contraction accomplished by the sliding of myosin filaments passed the actin filament ...
Enzymes
... Protease — breaking down of proteins Cellulase — breaking down of fiber (cellulose) Amylase — breaking down of starch (amylose) Lactase — breaking down of dairy products (lactose) Sucrase — breaking down of sugar (sucrose) Maltase — breaking down of grains (maltose) ...
... Protease — breaking down of proteins Cellulase — breaking down of fiber (cellulose) Amylase — breaking down of starch (amylose) Lactase — breaking down of dairy products (lactose) Sucrase — breaking down of sugar (sucrose) Maltase — breaking down of grains (maltose) ...
Function of ribosomes and glutamyl-tRNA isoacceptors
... per ml. To determine the codon responses of the glutamyl-tRNA isoacceptors, reaction mixtures contained the trinucleotide codon GAA or GAG (kindly supplied by Dr. Dolph Hatfield, National Cancer Institute). Other reaction conditions are indicated in the legends to Tables II and III. The peptidyltran ...
... per ml. To determine the codon responses of the glutamyl-tRNA isoacceptors, reaction mixtures contained the trinucleotide codon GAA or GAG (kindly supplied by Dr. Dolph Hatfield, National Cancer Institute). Other reaction conditions are indicated in the legends to Tables II and III. The peptidyltran ...
Identification of fungal oxaloacetate hydrolyase within the
... ability of fungi to produce oxalate with the presence of an oah gene in their genome. This task was made difficult by the fact that fungal genomes encode several OAH homologs having an unusually high level of shared sequence identity. Thus, identification of OAH activity in the gene product would re ...
... ability of fungi to produce oxalate with the presence of an oah gene in their genome. This task was made difficult by the fact that fungal genomes encode several OAH homologs having an unusually high level of shared sequence identity. Thus, identification of OAH activity in the gene product would re ...
Osmoadaptative Strategy and Its Molecular
... Halophilic microbes living in hypersaline environments must counteract the detrimental effects of low water activity and salt interference. Some halophilic prokaryotes equilibrate their intracellular osmotic strength with the extracellular milieu by importing inorganic solutes, mainly potassium. The ...
... Halophilic microbes living in hypersaline environments must counteract the detrimental effects of low water activity and salt interference. Some halophilic prokaryotes equilibrate their intracellular osmotic strength with the extracellular milieu by importing inorganic solutes, mainly potassium. The ...
Lecture Notes Ch21
... – The active site has a rigid shape – Only substrates with the matching shape can fit – The substrate is a key that fits the lock of the active site ...
... – The active site has a rigid shape – Only substrates with the matching shape can fit – The substrate is a key that fits the lock of the active site ...
Recombinant expression and characterisation of monofunctional S-
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
Dreze et al, Methods Enzymol 2010
... functional as truncated fragments and not when expressed in the context of full-length proteins (de novo autoactivators). Both genuine transcription factors and cognate DB-X autoactivators can be identified and removed by performing prescreens for reporter gene activation either with AD expressed al ...
... functional as truncated fragments and not when expressed in the context of full-length proteins (de novo autoactivators). Both genuine transcription factors and cognate DB-X autoactivators can be identified and removed by performing prescreens for reporter gene activation either with AD expressed al ...
application of hydroxyapatite in protein purification
... which is attributed to the vibrational modes as the formation CO32- by the reaction of adsorbed CO2. The incorporation of CO2 into CaHAp exist only as CO32[3]. The bands around 1515 cm-1 can be assigned to varios C-O stretching vibration modes of CO32- ions, substituted at phosphate site (type A) be ...
... which is attributed to the vibrational modes as the formation CO32- by the reaction of adsorbed CO2. The incorporation of CO2 into CaHAp exist only as CO32[3]. The bands around 1515 cm-1 can be assigned to varios C-O stretching vibration modes of CO32- ions, substituted at phosphate site (type A) be ...
32. Nutrient assimilation.pptx
... the 3 domains -> deep evolutionary roots near the origin of life Some workers argue for very few ancestral transport genes a few ion and organic-molecule transporters that diversified to give rise to over 1,000 transport genes in some eukaryotes. ...
... the 3 domains -> deep evolutionary roots near the origin of life Some workers argue for very few ancestral transport genes a few ion and organic-molecule transporters that diversified to give rise to over 1,000 transport genes in some eukaryotes. ...
Regulation of pyruvate, orthophosphate dikinase by ADP
... scenario since replacement of the regulatory Thr with the monoanionic (1-) side chain of Asp or Glu abolishes both enzyme activity and phosphorylatability (Table 2). Alternatively, the inactivation mechanism may also include a steric component in addition to an electrostatic one since substitution o ...
... scenario since replacement of the regulatory Thr with the monoanionic (1-) side chain of Asp or Glu abolishes both enzyme activity and phosphorylatability (Table 2). Alternatively, the inactivation mechanism may also include a steric component in addition to an electrostatic one since substitution o ...
PowerPoint 簡報
... and specificity of enzymes Binding energy (GB)--- the energy derived from enzyme-substrate interaction 1. Much of the catalytic power of enzymes is ultimately derived from the free energy released in forming multiple weak bonds and interactions between an enzyme and its substrate. This binding ener ...
... and specificity of enzymes Binding energy (GB)--- the energy derived from enzyme-substrate interaction 1. Much of the catalytic power of enzymes is ultimately derived from the free energy released in forming multiple weak bonds and interactions between an enzyme and its substrate. This binding ener ...
Bovine Peptidoglycan Recognition Protein
... insects to humans. Various PGRPs are reported to have diverse functions: they bind bacterial molecules, digest PGN, and are essential to the Toll pathway in Drosophila. One family member, bovine PGN recognition protein-S (bPGRP-S), has been found to bind and kill microorganisms in a PGN-independent ...
... insects to humans. Various PGRPs are reported to have diverse functions: they bind bacterial molecules, digest PGN, and are essential to the Toll pathway in Drosophila. One family member, bovine PGN recognition protein-S (bPGRP-S), has been found to bind and kill microorganisms in a PGN-independent ...
Dear teacher/student
... >nameofyoursequence. You are now using the so called FastA-format, without which the search engine will not work. It is important to be precise, as the BLAST software is prone to inconsistent input. 4. Proceed by selecting the database that you would like to search. SwissProt (Swiss protein) is the ...
... >nameofyoursequence. You are now using the so called FastA-format, without which the search engine will not work. It is important to be precise, as the BLAST software is prone to inconsistent input. 4. Proceed by selecting the database that you would like to search. SwissProt (Swiss protein) is the ...
The amino acid profiles of the yolk and albumen of domestic duck
... samples. Glu and Asp were the most abundant AA in both the yolk and the albumen with respective values (g/100 g crude protein, cp) of : 13.4-12.6 (Glu) and 10.12-10.09 (Asp). A look at Table I will show that AA in the albumen was slightly more concentrated (on pair wise comparisons) than the corresp ...
... samples. Glu and Asp were the most abundant AA in both the yolk and the albumen with respective values (g/100 g crude protein, cp) of : 13.4-12.6 (Glu) and 10.12-10.09 (Asp). A look at Table I will show that AA in the albumen was slightly more concentrated (on pair wise comparisons) than the corresp ...
PathogenBioinformatics
... This table lists proteins upstream and downstream of the target protein LLO. Each protein is labeled with its function and location on the strain (beginning and ending base pair numbers). In the right-most column labeled “Aliases” are links to other bioinformatics sites. Notice the column heading "f ...
... This table lists proteins upstream and downstream of the target protein LLO. Each protein is labeled with its function and location on the strain (beginning and ending base pair numbers). In the right-most column labeled “Aliases” are links to other bioinformatics sites. Notice the column heading "f ...
In-situ Protocol for larval/pupal/adult tissues
... 1. Section samples in 10-20 µM thick sections on a cryostat Remember to be RNase conscious during this procedure. Try not to introduce RNase to the samples and limit the activity of native RNases. Use only new tools that have been washed with RNase Zap or RNase AWAY or a similar product. Section as ...
... 1. Section samples in 10-20 µM thick sections on a cryostat Remember to be RNase conscious during this procedure. Try not to introduce RNase to the samples and limit the activity of native RNases. Use only new tools that have been washed with RNase Zap or RNase AWAY or a similar product. Section as ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.