Metabolomics - Circulation: Cardiovascular Genetics
... isocitrate dehydrogenases (IDH1 and IDH2) have been found to promote glioma, acute myeloid leukemia, chondrosarcoma, and other tumours. In the traditional conception, acetyl-CoA is thought to progress unidirectionally through the CAC. Instead, IDH1 (primarily cytoplasmic and NADP+ dependant) and IDH ...
... isocitrate dehydrogenases (IDH1 and IDH2) have been found to promote glioma, acute myeloid leukemia, chondrosarcoma, and other tumours. In the traditional conception, acetyl-CoA is thought to progress unidirectionally through the CAC. Instead, IDH1 (primarily cytoplasmic and NADP+ dependant) and IDH ...
Natural abundance of 15N in amino acids and
... and 4-aminobutyraldehyde, and (3) reduction of the intermediate to form the final product homospd using the NADH produced by process (1). The exceedingly high enrichment of d15N in homospd molecules separated from nodules and the contrasting small enrichment of those from rhizobia ( Table 4) suggest ...
... and 4-aminobutyraldehyde, and (3) reduction of the intermediate to form the final product homospd using the NADH produced by process (1). The exceedingly high enrichment of d15N in homospd molecules separated from nodules and the contrasting small enrichment of those from rhizobia ( Table 4) suggest ...
21. Toshihiko Okamotofi`1 Yo Isogai,$2 and T6111 K0izumi*1 :
... by the plant for synthesising proteins for growth. A number of comprehensive reviews discussing the N assimilation system have been published (Lam et al. 1996; McAllister et al. 2012; Xu et al. 2012). Briefly, NO3− is reduced to NO2− by nitrate reductase (NR) in the cytoplasm (Lea et al. 2006) and f ...
... by the plant for synthesising proteins for growth. A number of comprehensive reviews discussing the N assimilation system have been published (Lam et al. 1996; McAllister et al. 2012; Xu et al. 2012). Briefly, NO3− is reduced to NO2− by nitrate reductase (NR) in the cytoplasm (Lea et al. 2006) and f ...
Vitamin C
... inactivate both endo-and exogenous substances. • Endogenous: Include various hormones and steroids (cholesterol) • Exogenous: xenobiotics (foreign chemicals) drugs, carcinogens, pesticides, pollutants, food additives • The reactions to metabolize these substances usually involve hydroxylation's foll ...
... inactivate both endo-and exogenous substances. • Endogenous: Include various hormones and steroids (cholesterol) • Exogenous: xenobiotics (foreign chemicals) drugs, carcinogens, pesticides, pollutants, food additives • The reactions to metabolize these substances usually involve hydroxylation's foll ...
Regulation of intermediary metabolism by protein acetylation
... Interestingly, the acetylated proteins identified from human liver tissue overlap more with those identified from mouse liver than those identified in human lymphocytes, suggesting distinct patterns of acetylation in different tissues that are evolutionarily conserved. Together, these three acetylat ...
... Interestingly, the acetylated proteins identified from human liver tissue overlap more with those identified from mouse liver than those identified in human lymphocytes, suggesting distinct patterns of acetylation in different tissues that are evolutionarily conserved. Together, these three acetylat ...
1 A multi-platform metabolomics approach identifies novel
... Succinate and lactate abundance are shown in panel E of Fig 2. Succinate levels, and the succinate:lactate ratio have historically been associated with BV (21-23), and succinate has been postulated to play an immunomodulatory role (23). Here we show that succinate is not associated with bacterial di ...
... Succinate and lactate abundance are shown in panel E of Fig 2. Succinate levels, and the succinate:lactate ratio have historically been associated with BV (21-23), and succinate has been postulated to play an immunomodulatory role (23). Here we show that succinate is not associated with bacterial di ...
Full Text - J
... amino acid sequence information, degenerate RTPCR and 5′- and 3′-rapid amplification of cDNA ends (RACE) experiments were performed that finally revealed the 5′- and 3′-termini sequences of the full-length cDNA (1600 bp) that encodes both CrTX-A and CrTX-B. Histopathologic studies, clearly showed th ...
... amino acid sequence information, degenerate RTPCR and 5′- and 3′-rapid amplification of cDNA ends (RACE) experiments were performed that finally revealed the 5′- and 3′-termini sequences of the full-length cDNA (1600 bp) that encodes both CrTX-A and CrTX-B. Histopathologic studies, clearly showed th ...
as a PDF
... to lactate. The reaction mixture consisted of O.lSmmoll" 1 NADH, l m m o l F 1 KCN, SOmmolP 1 imidazole, 2.5mmoin 1 pyruvate, pH7.2 at 25°C. Citrate synthase (CS; EC 4.1.3.7). The reduction of DTNB [5,5'-dithiobis(2nitrobenzoic acid)] by free coenzyme A was monitored at 412 nm. The reaction mixture ...
... to lactate. The reaction mixture consisted of O.lSmmoll" 1 NADH, l m m o l F 1 KCN, SOmmolP 1 imidazole, 2.5mmoin 1 pyruvate, pH7.2 at 25°C. Citrate synthase (CS; EC 4.1.3.7). The reduction of DTNB [5,5'-dithiobis(2nitrobenzoic acid)] by free coenzyme A was monitored at 412 nm. The reaction mixture ...
Propionate metabolism in Saccharomyces cerevisiae
... Aerobic, glucose-limited chemostat of Saccharomyces cerevisiae CBS 8066 cometabolized propionate when this compound was added t o the reservoir medium. Co-metabolism of propionate led to an increase of the biomass and protein yields. Attempts to grow 5. cerevisiae on propionate as a sole source of c ...
... Aerobic, glucose-limited chemostat of Saccharomyces cerevisiae CBS 8066 cometabolized propionate when this compound was added t o the reservoir medium. Co-metabolism of propionate led to an increase of the biomass and protein yields. Attempts to grow 5. cerevisiae on propionate as a sole source of c ...
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill
... as ERRa, NRF1, NRF2 and PPARs [14,15]. PGC-1a null mice showed muscle dysfunction, abnormal weight control and hepatic steatosis [16,17]. Although skeletal muscle was not an active lipogenic organ as liver, exercise training or AMPK activation has been also reported to promote fatty acid synthesis a ...
... as ERRa, NRF1, NRF2 and PPARs [14,15]. PGC-1a null mice showed muscle dysfunction, abnormal weight control and hepatic steatosis [16,17]. Although skeletal muscle was not an active lipogenic organ as liver, exercise training or AMPK activation has been also reported to promote fatty acid synthesis a ...
DESIGN, SYNTHESIS AND ANTIMICROBIAL SCREENING OF AMINO ACIDS CONJUGATED 2 AMINO4ARYLTHIAZOLE DERIVATIVES
... antimicrobial agents with clinically unexploited mode of action. 1 Thiazole is a versatile bioactive heterocycle with biocidal S‐C=N entity having their wide presence in many synthetic drugs of several infective diseases such as allergies,2 malaria, 3 inflammation, 4 fungal and bac ...
... antimicrobial agents with clinically unexploited mode of action. 1 Thiazole is a versatile bioactive heterocycle with biocidal S‐C=N entity having their wide presence in many synthetic drugs of several infective diseases such as allergies,2 malaria, 3 inflammation, 4 fungal and bac ...
(LPT1) and humans (LPCAT3)
... I would like to express my gratitude for my advisor, Dr. Peter Oelkers, Dept. of Bioscience and Biotechnology, Drexel University. Under his guidance I learned lot of lab techniques and thus acquired tools to answer research questions and think like a scientist. He taught me all. I don’t remember one ...
... I would like to express my gratitude for my advisor, Dr. Peter Oelkers, Dept. of Bioscience and Biotechnology, Drexel University. Under his guidance I learned lot of lab techniques and thus acquired tools to answer research questions and think like a scientist. He taught me all. I don’t remember one ...
DIFFERENCES IN ENZYME CONTENT OF AZUROPHIL AND
... lysosomal enzymes (acid phosphatase, arylsulfatase, ~-galactosidase, /3-glucuronidase, esterase, and 5r-nucleotidase) corresponded to that of azurophil granules. Progranulocytes contained numerous reactive granules, and later stages contained only a few. The distribution of one enzyme, alkaline phos ...
... lysosomal enzymes (acid phosphatase, arylsulfatase, ~-galactosidase, /3-glucuronidase, esterase, and 5r-nucleotidase) corresponded to that of azurophil granules. Progranulocytes contained numerous reactive granules, and later stages contained only a few. The distribution of one enzyme, alkaline phos ...
- Wiley Online Library
... [1-13C]aspartate peaks, consistent with observations in the perfused mouse liver.4 Because the conversion of OAA to malate and aspartate are reversible reactions, there is 13C label exchange between these three metabolites. In addition, reversible dehydration of [1-13C]malate to [1-13C]fumarate, cat ...
... [1-13C]aspartate peaks, consistent with observations in the perfused mouse liver.4 Because the conversion of OAA to malate and aspartate are reversible reactions, there is 13C label exchange between these three metabolites. In addition, reversible dehydration of [1-13C]malate to [1-13C]fumarate, cat ...
Intraplastidic Localization of the Enzymesthat Convert Cucumber
... Laboratory of Plant Pigment Biochemistry and Photobiology, 202 ABL, University of Illinois, Urbana, Illinois 61801 these enzymes is more controversial. On the basis of osmotic lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that ...
... Laboratory of Plant Pigment Biochemistry and Photobiology, 202 ABL, University of Illinois, Urbana, Illinois 61801 these enzymes is more controversial. On the basis of osmotic lysis of crude etiochloroplast preparations accompanied by differential centrifugation, Smith and Rebeiz (27) proposed that ...
Document
... sensitivity detected residual activity not only for rBla g 2 MutF2 but also for rBla g 2 Wt. The crystal structure of rBla g 2 Wt (N93Q) revealed a self-inhibited state of the protein, which is due to a unique insertion in the flap region of the molecule, which partially occupies the substrate bindi ...
... sensitivity detected residual activity not only for rBla g 2 MutF2 but also for rBla g 2 Wt. The crystal structure of rBla g 2 Wt (N93Q) revealed a self-inhibited state of the protein, which is due to a unique insertion in the flap region of the molecule, which partially occupies the substrate bindi ...
Analysis of the Compartmentation of Glycolytic
... of the information on subcellular metabolism in tubers has come indirectly from the use of transgenic plants (e.g. Müller-Röber et al., 1992; Stark et al., 1992; Sweetlove et al., 1996; Tjaden et al., 1998; Trethewey et al., 1999; Tauberger et al., 2000). In this study, we addressed three major op ...
... of the information on subcellular metabolism in tubers has come indirectly from the use of transgenic plants (e.g. Müller-Röber et al., 1992; Stark et al., 1992; Sweetlove et al., 1996; Tjaden et al., 1998; Trethewey et al., 1999; Tauberger et al., 2000). In this study, we addressed three major op ...
Differential effects of heptanoate and hexanoate on myocardial citric
... adjusted to give interventricular venous oxygen saturation of 35– 45%. A continuous infusion of [U-14C]glucose (0.2 Ci/min) was initiated 30 min before ischemia into the proximal end of the coronary perfusion line at a rate of 0.1 ml/min. Regional myocardial ischemiareperfusion was induced in the L ...
... adjusted to give interventricular venous oxygen saturation of 35– 45%. A continuous infusion of [U-14C]glucose (0.2 Ci/min) was initiated 30 min before ischemia into the proximal end of the coronary perfusion line at a rate of 0.1 ml/min. Regional myocardial ischemiareperfusion was induced in the L ...
Protein Proteinase Inhibitors in Male Sex Glands
... resulting inhibitor preparations was about 60%, calculated from the specific activity of desalted and lyophilized fractions [4, 5]. The inhibitor material was subsequently chromatographed on Sulfoethyl Sephadex, using an ammoniumacetate concentration gradient with constant p H 5.4 (Fig. 1). In the m ...
... resulting inhibitor preparations was about 60%, calculated from the specific activity of desalted and lyophilized fractions [4, 5]. The inhibitor material was subsequently chromatographed on Sulfoethyl Sephadex, using an ammoniumacetate concentration gradient with constant p H 5.4 (Fig. 1). In the m ...
Biotechnology and Bioengineering
... at alpha subunit (TouA) positions F176 and F196 among which nine were novel: F176H, F176N, F176S, F176T, F196A, F196L, F196T, F196Y, F196H, F196I, and F196V. By testing the substrates phenol, toluene, and naphthalene, these positions were found to influence ToMO oxidation activity and regiospecifici ...
... at alpha subunit (TouA) positions F176 and F196 among which nine were novel: F176H, F176N, F176S, F176T, F196A, F196L, F196T, F196Y, F196H, F196I, and F196V. By testing the substrates phenol, toluene, and naphthalene, these positions were found to influence ToMO oxidation activity and regiospecifici ...
Regio- and Enantioselective Alkane Hydroxylation with Engineered Cytochromes P450 BM-3 Peter Meinhold,
... a Initial NADPH consumption rates were measured over 15 s at 340 nm as nmol NADPH/min/nmol protein. Octane reactions contained 100 nM P450, 166 µM NADPH, and 4 mM octane in 1% ethanol and potassium phosphate buffer. Propane reactions contained 200 nM P450, 166 µM NADPH, and propane-saturated potassi ...
... a Initial NADPH consumption rates were measured over 15 s at 340 nm as nmol NADPH/min/nmol protein. Octane reactions contained 100 nM P450, 166 µM NADPH, and 4 mM octane in 1% ethanol and potassium phosphate buffer. Propane reactions contained 200 nM P450, 166 µM NADPH, and propane-saturated potassi ...
R Is for Arginine Metabolism of Arginine Takes off Again, in New
... concentrations may be limiting for NOS in specific subcellular locales. Moreover, the presence of endogenous competitive inhibitors of NOS (discussed below) may raise the effective intracellular arginine concentration needed for full enzyme activity. In any event, the action of NOS on l-arginine yie ...
... concentrations may be limiting for NOS in specific subcellular locales. Moreover, the presence of endogenous competitive inhibitors of NOS (discussed below) may raise the effective intracellular arginine concentration needed for full enzyme activity. In any event, the action of NOS on l-arginine yie ...
Taurine 10 sample chapter
... associated with a fall in the islet cell expression of IGF-II (Petril et al. 1998). IGF-II was shown to function as an islet survival factor in vitro. The induction of islet cell apoptosis in vivo may involve an increased expression of inducible nitric oxide synthase (iNOS) within ß cells. Interesti ...
... associated with a fall in the islet cell expression of IGF-II (Petril et al. 1998). IGF-II was shown to function as an islet survival factor in vitro. The induction of islet cell apoptosis in vivo may involve an increased expression of inducible nitric oxide synthase (iNOS) within ß cells. Interesti ...
R Is for Arginine
... concentrations may be limiting for NOS in specific subcellular locales. Moreover, the presence of endogenous competitive inhibitors of NOS (discussed below) may raise the effective intracellular arginine concentration needed for full enzyme activity. In any event, the action of NOS on l-arginine yie ...
... concentrations may be limiting for NOS in specific subcellular locales. Moreover, the presence of endogenous competitive inhibitors of NOS (discussed below) may raise the effective intracellular arginine concentration needed for full enzyme activity. In any event, the action of NOS on l-arginine yie ...