Download MOLECULAR BIOLOGY EXAM II

MOLECULAR BIOLOGY EXAM II
February 19, 2003
______________________________
Good luck! Remember, a drawing or figure is often more valuable than words. Tables are great. As always, I
will "sell" you part of the answer if you need it. Make all answers fit in the spaces given.
I
Fill in (3 pts per space.)
1,2)
3)
4)
5,6)
7)
8)
9,10)
Small non-coding RNA molecules found in the nucleus are called
___________________ and _____________________.
Design a 12 base (single stranded) probe for Northern Blot, using the following amino
acid sequence: leu-met-gly-phe-ser-glu-asp met-trp ____________________.
____________________ enzyme is used to make a cDNA library from the mRNA.
The stringency of hybridization can be increased or decreased by altering
__________________________ and/or ____________________.
A screening tool in which many DNA sequences are placed on a chip for hybridization
analysis. ______________________
___________ is the term used to define an area of sequence which codes for protein,
and is not interrupted by stop codons.
Protein __________________ are often defined by ______________ in the gene.
II
5 points each. Choose 4 to describe/draw in several sentences.
linkage analysis
shotgun sequencing
pcr
SNP
microsatelites
chromosome walking
III
1)
Essay questions
10 points
Describe the three main types of RNA. Draw two of them.
2)
15 points
Describe physical and genetic mapping, discussing the following;
What is the basis for each type?
What types of techniques are used in each type of mapping? (list several)
What type of data is obtained?
Why are both techniques necessary? (how do they complement each other?)
3)
10 points
Describe the function of each of these enzymes, and indicate when and
why you might use them in lab:
DNA polymerase
ligase
nuclease
4)
10 points
research study
You have identified a new protein found in liver, and have determined its sequence. The protein
is an enzyme that functions in repairing cellular damage by toxins. You have developed a
simple assay to test the presence and activity of this enzyme. You know that the possibilities for
using this enzyme in medicine are enormous.
You need to know- What is its gene sequence? Where is it made? Do other organisms make
this protein? Are the sequences from other organisms similar? Is it always made or only at
certain times? How is the gene regulated? You have three people working for you, all are
pretty handy in the lab. Outline a strategy for each to begin tackling one of these questions, (or
another critical issue you think is important to market this protein). Be sure the three areas will
provide information that is complementary, and provides important info. Outline in flow chart
form how each person will achieve their goal, including brief info about techniques used, and
the type of data they will obtain. You can use the back of this sheet. THINK before you write
Strategize.
5)
5 points
Make up and answer a very good question. If in doubt- ask me!
Samples: Compare and contrast the use of RFLP and PCR analysis in forensic analysis;
RNA modification; DNA purification