Download 12-1 pm Location: Room HSW1057 UCSF

Lunch & Learn
Seminar
A business of Merck KGaA, Darmstadt, Germany
Detect RNA the smart way with SmartFlare
TM
Live Cell Biomarker Detection for Imaging and
Sorting Applications
Presented By: Antonia Livolsi, Ph.D, Research Application Scientist
Traditional methods for examining
gene expression involve lysed or fixed
cell populations. The ability to do so
in live cells would allow for more
physiologically relevant information
based on a cell’s response to given
stimuli. Determining which genes are
up or down regulated in those cells
provides insight into complex gene
regulatory networks and cell function.
Here we present SmartFlare, a
technology capable of detecting
specific mRNA and microRNA in live
cells. This technology allows for
carrier-free uptake of the reagent,
followed by detection of target RNA at
the single cell level, with the ability to
perform downstream analysis in the
same sample. The reagent has no
toxic effect on cells and we have
demonstrated the ability to isolate
and further propagate live cell
populations based on gene expression
levels.
Date: Thursday May 26
Time: 12-1 pm
Location: Room HSW1057
UCSF-513 Parnassus Ave
Please RSVP to:
Richard Driver
[email protected]
Tel: 408 429-0668