Xenopus Spinal Neurons Express Kv2 Potassium Channel
... yielding only one amino acid change (Fig. 1B). Because most of the nucleotide differences between the PCR products and the library cDNAs conserve the ainino acid sequence, it is unlikely that the PCR variants are attributable to random errors introduced by reverse transcriptase or Taq polymerase. It ...
... yielding only one amino acid change (Fig. 1B). Because most of the nucleotide differences between the PCR products and the library cDNAs conserve the ainino acid sequence, it is unlikely that the PCR variants are attributable to random errors introduced by reverse transcriptase or Taq polymerase. It ...
Biosynthesis of geranial, a potent aroma compound in ginger
... (5′-CCG AAT TCC ATA TGA TGG CAG AGC TGG GA-3′ and 5′-ACG CGT CGA CCT CTC TTA CGC TTC AGT CAA3′) using proofreading KOD-plus polymerase (Toyobo, Inc., Osaka, Japan). These primers included the NdeI and SalI restriction endonuclease recognition sites, respectively. The PCR products were digested by th ...
... (5′-CCG AAT TCC ATA TGA TGG CAG AGC TGG GA-3′ and 5′-ACG CGT CGA CCT CTC TTA CGC TTC AGT CAA3′) using proofreading KOD-plus polymerase (Toyobo, Inc., Osaka, Japan). These primers included the NdeI and SalI restriction endonuclease recognition sites, respectively. The PCR products were digested by th ...
SUPPORTING FILE S1 SUPPLEMENTARY MATERIAL AND
... Tricarballylic acid (15 mmol L-1) buffer pH 7.8, MgCl2 (10 mmol L-1), 10 % glycerol. RNase ...
... Tricarballylic acid (15 mmol L-1) buffer pH 7.8, MgCl2 (10 mmol L-1), 10 % glycerol. RNase ...
MITOCHONDRIAL DNA REPLICATION IN PRE
... 1.4.4.2 Interaction between multiple wild type mitochondrial genomes......................42 1.4.4.3 NT-derived ESCs..............................................................................................43 1.4.4.4 Cross-species NT............................................................... ...
... 1.4.4.2 Interaction between multiple wild type mitochondrial genomes......................42 1.4.4.3 NT-derived ESCs..............................................................................................43 1.4.4.4 Cross-species NT............................................................... ...
Analysis and engineering of acetyl
... strating that the natifie cytosolic acetyl-CoA synthesis pathflay can be ffflly replaced, Chapter 3 also refiealed targets that need to be addressed to achiefie optimal in vivo performance of the alternatifie reactions for sffpply of cytosolic acetyl-CoA. Design and implementation of metabolic engineering st ...
... strating that the natifie cytosolic acetyl-CoA synthesis pathflay can be ffflly replaced, Chapter 3 also refiealed targets that need to be addressed to achiefie optimal in vivo performance of the alternatifie reactions for sffpply of cytosolic acetyl-CoA. Design and implementation of metabolic engineering st ...
Function and Control of the Spx-Family of Proteins Within
... expression machinery (de Hoon et al. 2010; Piggot and Hilbert 2004). Most notable among these are the spore-specific RNA polymerase forms bearing unique σ subunits (Stragier and Losick 1990). The assembly of alternative RNA polymerase forms, bearing spore-specific σ subunits, is the result of a sign ...
... expression machinery (de Hoon et al. 2010; Piggot and Hilbert 2004). Most notable among these are the spore-specific RNA polymerase forms bearing unique σ subunits (Stragier and Losick 1990). The assembly of alternative RNA polymerase forms, bearing spore-specific σ subunits, is the result of a sign ...
Characterization of an Immuno-dominant Variable Surface
... More recently several other probes, based either on DNA sequences (27-29), or the detection of specific antigens (30), have been suggested as diagnostic reagents . However, in most cases, axenic cultivation (20) and cloning (31) of amoebae directly from fresh stool samples before assay with any of t ...
... More recently several other probes, based either on DNA sequences (27-29), or the detection of specific antigens (30), have been suggested as diagnostic reagents . However, in most cases, axenic cultivation (20) and cloning (31) of amoebae directly from fresh stool samples before assay with any of t ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... and provisional application Serial No. 60/269,944, ?led Feb. ...
... and provisional application Serial No. 60/269,944, ?led Feb. ...
Global transcriptional control by glucose and
... B. anthracis are attenuated on virulence in animal models of infection (24,28). The coordinate CcpA-dependent regulation of virulence and carbon utilization genes might be critical for fitness when pathogens compete with other microbes for niche colonization (23). Recently, we have demonstrated that ...
... B. anthracis are attenuated on virulence in animal models of infection (24,28). The coordinate CcpA-dependent regulation of virulence and carbon utilization genes might be critical for fitness when pathogens compete with other microbes for niche colonization (23). Recently, we have demonstrated that ...
Cloning and sequencing of the kedarcidin biosynthetic
... (ii) the (R)-2-aza-3-chloro-b-tyrosine moiety that has not been seen in any other natural product, (iii) a deceivingly simple isopropoxy group at the 2-naphthonate moiety, the biosynthesis of which has little literature precedence, and (iv) the peripheral moieties are appended to the enediyne core w ...
... (ii) the (R)-2-aza-3-chloro-b-tyrosine moiety that has not been seen in any other natural product, (iii) a deceivingly simple isopropoxy group at the 2-naphthonate moiety, the biosynthesis of which has little literature precedence, and (iv) the peripheral moieties are appended to the enediyne core w ...
SPT4, a gene important for tr
... Plasmids. The plasmids pJF17, pJF42, and pBM25 contain subclones of the cloned SPT4 gene in the centromere-containing vector YCp50 (Johnston and Davis 1984) and were used to delimit the SPT4 gene (Fig. 1). The HindIII fragment that contains SPT4 function was cloned in the integrating vector YIp5 (St ...
... Plasmids. The plasmids pJF17, pJF42, and pBM25 contain subclones of the cloned SPT4 gene in the centromere-containing vector YCp50 (Johnston and Davis 1984) and were used to delimit the SPT4 gene (Fig. 1). The HindIII fragment that contains SPT4 function was cloned in the integrating vector YIp5 (St ...
Landick R, Yanofsky C. 1987. Transcription
... attenuator. This decision is based on the cellular level of charged tRNA Trp. Under starvation conditions, in which all tRNATrp essentially is uncharged, the rate of read through transcription at the attenuator increases about eightfold over the rate observed when tRNATrp is fully charged. We do not ...
... attenuator. This decision is based on the cellular level of charged tRNA Trp. Under starvation conditions, in which all tRNATrp essentially is uncharged, the rate of read through transcription at the attenuator increases about eightfold over the rate observed when tRNATrp is fully charged. We do not ...
Pseudomon-1 motif
... indeed find many gene-independent motifs, but we additionally found many geneassociated motifs, e.g., the msiK motif. It may seem surprising that gene-associated motifs like msiK were not detected by the previous pipeline, given that the previous pipeline was designed to find such motifs. The follow ...
... indeed find many gene-independent motifs, but we additionally found many geneassociated motifs, e.g., the msiK motif. It may seem surprising that gene-associated motifs like msiK were not detected by the previous pipeline, given that the previous pipeline was designed to find such motifs. The follow ...
Figure legends Figure 1. Biosynthesis and catabolism of NAD+ in
... response to quinolinate treatment. Additionally, the relative levels of pathogen related and NAD metabolism transcripts were also examined by RT-qPCR, using ACT2 as an internal control. AO was induced during bacterial infection (C). For PR1, the relative transcript levels detected in the different l ...
... response to quinolinate treatment. Additionally, the relative levels of pathogen related and NAD metabolism transcripts were also examined by RT-qPCR, using ACT2 as an internal control. AO was induced during bacterial infection (C). For PR1, the relative transcript levels detected in the different l ...
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in
... in A. aeolicus and Tc. ruber. Based either on the whole genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, ...
... in A. aeolicus and Tc. ruber. Based either on the whole genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, ...
Engineering of polyketide biosynthetic pathways for bioactive
... career. Without his instructions, it would have been impossible for me to get so much work done in four years and finish this dissertation. I would also like to thank my committee members, Dr. Randy Lewis, Dr. David Britt, Dr. Dong Chen and Dr. Foster Agblevor for their advice and feedback, which he ...
... career. Without his instructions, it would have been impossible for me to get so much work done in four years and finish this dissertation. I would also like to thank my committee members, Dr. Randy Lewis, Dr. David Britt, Dr. Dong Chen and Dr. Foster Agblevor for their advice and feedback, which he ...
Functional Characterization of Nine Norway
... Far, PaTPS-Lin, PaTPS-Bis, and PaTPS-Pin) were isolated by cDNA library filter hybridization as described in Fäldt et al. (2003b). Combination of similarity-based PCR strategies previously developed for isolation of conifer TPS genes (Bohlmann et al., 1997), mining of expressed sequence tags, and R ...
... Far, PaTPS-Lin, PaTPS-Bis, and PaTPS-Pin) were isolated by cDNA library filter hybridization as described in Fäldt et al. (2003b). Combination of similarity-based PCR strategies previously developed for isolation of conifer TPS genes (Bohlmann et al., 1997), mining of expressed sequence tags, and R ...
The Plant Cell
... The alf5 allele has a 29-bp deletion within a coding sequence identified in the genomic region covered by the 67X1 transgene, shown in Figure 5. After sequencing the 6.1-kb 67X1 genomic clone, only a single coding sequence within 67X1 could be inferred by homology with available GenBank sequence ent ...
... The alf5 allele has a 29-bp deletion within a coding sequence identified in the genomic region covered by the 67X1 transgene, shown in Figure 5. After sequencing the 6.1-kb 67X1 genomic clone, only a single coding sequence within 67X1 could be inferred by homology with available GenBank sequence ent ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.