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Download Imaging cellular acylation Rami N. Hannoush Genentech, Inc
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Transcript
S005 Imaging cellular acylation Rami N. Hannoush Genentech, Inc., South San Francisco, USA Wnts are secreted palmitoylated glycoproteins that are important in embryonic development and human cancers. Here we report a method for imaging palmitoylated forms of Wnt proteins with subcellular resolution by using clickable bio-orthogonal fatty acid probes. Palmitoylated Wnt3a is visualized in various subcellular compartments, shedding light onto its trafficking mechanism. Moreover, the dynamics of Wnt palmitoylation are investigated, and reveal the role of palmitoylation in Wnt secretion and signaling. Overall, our method is broadly applicable to imaging other palmitoylated cellular proteins, and it has utility in drug discovery for identifying specific modulators of protein palmitoylation.
