Download Transformation Pre-Lab

Transformations Lab
Pre-Lab
Plasmid:
Selectable Marker:
Color Marker:
Phenotype of transformants:
1. Give an example of how the transfer of DNA into a new organism has led to
an improvement in everyday life.
2. Explain the significance of Frederick Griffith’s work to transformation.
3. What is the “transforming principle?”
4. How is transformation defined today?
5. Give several examples of bacteria that transform naturally, AND explain how
they go through this process.
6. How would these bacteria select for DNA that is likely to be beneficial for
them?
7. Give an example of how it would be beneficial for a bacterium to bring in and
use DNA from other members of its species.
8. What does the word competent mean when referring to artificial
transformation?
9. How can we make cells competent, and what does that actually do to the cell?
10. What does the term “expression” refer to?
11. What is a plasmid and why are they good tools for genetic recombination?
12. What are the two problems that biotechnologists face when transforming
bacteria AND what can be done to overcome these issues?
13. Why is it necessary to have a “marker” when transforming bacteria?
14. Why are antibiotic resistant genes significant in transformation? Make sure
you explain this process in detail!
15. *Write (type) down any further questions you have.