Molecular characterization of a cluster of at least two
... 1 mM-EDTA and 200 pg heat-treated RNAase ml-I (Maniatis et al., 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of th ...
... 1 mM-EDTA and 200 pg heat-treated RNAase ml-I (Maniatis et al., 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of th ...
Molecular characterization of a cluster of at least two
... 1 mM-EDTA and 200 pg heat-treated RNAase ml-I (Maniatis et al., 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of th ...
... 1 mM-EDTA and 200 pg heat-treated RNAase ml-I (Maniatis et al., 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of th ...
c12) United States Patent - Rice Scholarship Home
... acids (Galkin eta!., 1997), chiral hydroxy acids, esters, alcohols, and other fine chemicals synthesized by different dehydrogenases (Hummel and Kula, 1989), (Tishkov eta!., 1999). Purified FDH has also been used to regenerate NADH in vitro for the industrial production of non-natural amino acids th ...
... acids (Galkin eta!., 1997), chiral hydroxy acids, esters, alcohols, and other fine chemicals synthesized by different dehydrogenases (Hummel and Kula, 1989), (Tishkov eta!., 1999). Purified FDH has also been used to regenerate NADH in vitro for the industrial production of non-natural amino acids th ...
The female-killing chromosome of the silkworm, Bombyx mori, was
... p50 and C108 strains are highly inbred strains and that have been maintained by sister–brother mating as described by Promboon et al. (1995). POS (p/p, os/W), PSCH (p/p, sch/W), POD (p/p, od/W), and PSCHOD (p/p, sch od/W) are marker strains having a normal W chromosome. The C137 strain is a highly i ...
... p50 and C108 strains are highly inbred strains and that have been maintained by sister–brother mating as described by Promboon et al. (1995). POS (p/p, os/W), PSCH (p/p, sch/W), POD (p/p, od/W), and PSCHOD (p/p, sch od/W) are marker strains having a normal W chromosome. The C137 strain is a highly i ...
Polyploidy
... • Triploid grass carp prefer pondweeds, • do not prefer plants such as cattail, water lily, etc. ...
... • Triploid grass carp prefer pondweeds, • do not prefer plants such as cattail, water lily, etc. ...
Analysis and engineering of acetyl
... Indffstrial biotechnology ffses microorganisms and enzymes to prodffce a flide range of chemical compoffnds, predominantly from carbohydrate-containing agricffltffral feedstocks. his approach has sefieral potential adfiantages ofier established petrochemical processes. Usage of fossil-based prodffts releases ca ...
... Indffstrial biotechnology ffses microorganisms and enzymes to prodffce a flide range of chemical compoffnds, predominantly from carbohydrate-containing agricffltffral feedstocks. his approach has sefieral potential adfiantages ofier established petrochemical processes. Usage of fossil-based prodffts releases ca ...
Yeast Supplemental Material
... CO2 saturation has a negative effect on yeast health and performance. The yeast essentially suffocates in high CO2 environments, and the uptake of nutrients is inhibited. It is important to note that it is not CO2 pressure alone which cause inhibition of yeast, but rather saturation of the yeast cel ...
... CO2 saturation has a negative effect on yeast health and performance. The yeast essentially suffocates in high CO2 environments, and the uptake of nutrients is inhibited. It is important to note that it is not CO2 pressure alone which cause inhibition of yeast, but rather saturation of the yeast cel ...
UNIVERSITAT ROVIRA I VIRGILI CODON USAGE ADAPTATION IN PROKARYOTIC GENOMES
... with the availability of a vast amount of sequences of proteins, genes and even genomes from all kinds of species, some of these hypotheses remain unchanged. From the limited nucleic acid sequences available, Grantham et al. (1) proposed the "genome hypothesis", postulating that genes in any given b ...
... with the availability of a vast amount of sequences of proteins, genes and even genomes from all kinds of species, some of these hypotheses remain unchanged. From the limited nucleic acid sequences available, Grantham et al. (1) proposed the "genome hypothesis", postulating that genes in any given b ...
TRANSLOCATIONS INVOLVING T H E THIRD AND THE FOURTH
... non-eyeless in appearance. However, in females carrying translocations, D and e, may be separatedby crossing over; hence, wild-type and D e, individuals appear along with D and e, in the progeny. The frequency of the crossing over between D and e, depends upon the distance between the locus of D and ...
... non-eyeless in appearance. However, in females carrying translocations, D and e, may be separatedby crossing over; hence, wild-type and D e, individuals appear along with D and e, in the progeny. The frequency of the crossing over between D and e, depends upon the distance between the locus of D and ...
Creating order from chaos: epigenome dynamics in
... activation of TEs (Lippman et al., 2003; Kato et al., 2004). Interestingly, in at least one ...
... activation of TEs (Lippman et al., 2003; Kato et al., 2004). Interestingly, in at least one ...
Genetics Practice Test
... ____ 30. Information from the Human Genome Project can be used to learn more about human diseases. _________________________ ____ 31. Animal breeders maintain cat and dog breeds by the process of hybridization. _________________________ ____ 32. Native Americans took teosine and used selective bree ...
... ____ 30. Information from the Human Genome Project can be used to learn more about human diseases. _________________________ ____ 31. Animal breeders maintain cat and dog breeds by the process of hybridization. _________________________ ____ 32. Native Americans took teosine and used selective bree ...
Virulence factors of Salmonella enterica serovar Enteritidis
... that despite numerous studies in the related serovar Typhimurium, little is known about the molecular interactions between serovar Enteritidis and the infected animal. The research described in this thesis was designed to address this topic and specifically focussed on systematic investigation of vi ...
... that despite numerous studies in the related serovar Typhimurium, little is known about the molecular interactions between serovar Enteritidis and the infected animal. The research described in this thesis was designed to address this topic and specifically focussed on systematic investigation of vi ...
Chromosome Variation
... only horse chromosomes from his mother. Additional reports of fertile female mules support the idea that their offspring inherit only horse chromosomes from their mother. When the father of a mule’s offspring is a horse, the offspring is horselike in appearance, because it apparently inherits horse ...
... only horse chromosomes from his mother. Additional reports of fertile female mules support the idea that their offspring inherit only horse chromosomes from their mother. When the father of a mule’s offspring is a horse, the offspring is horselike in appearance, because it apparently inherits horse ...
The landscape of Neandertal ancestry in present
... onto the modern human genetic background that were not tolerated and were swept away, in part because they contributed to male hybrid sterility. The resulting reduction in Neandertal ancestry was quantitatively large: in the fifth of the genome with highest B, Neandertal ancestry is 1.54 ± 0.15 time ...
... onto the modern human genetic background that were not tolerated and were swept away, in part because they contributed to male hybrid sterility. The resulting reduction in Neandertal ancestry was quantitatively large: in the fifth of the genome with highest B, Neandertal ancestry is 1.54 ± 0.15 time ...
Complete genome sequence of the prototype lactic acid bacterium
... genome of L. lactis IL1403 using the Projector software package (97) to order the contigs into large scaffolds. Standard PCR, followed by primer walk sequencing of the resulting products, was used to close the remaining 800 gaps, in combination with inverse PCR for the gaps for which no linkage info ...
... genome of L. lactis IL1403 using the Projector software package (97) to order the contigs into large scaffolds. Standard PCR, followed by primer walk sequencing of the resulting products, was used to close the remaining 800 gaps, in combination with inverse PCR for the gaps for which no linkage info ...
The Homothorax homeoprotein activates the nuclear localization of
... al. 1984; Salzberg et al. 1994, 1997). Thus, these mutations define the hth locus. Another lethal locus, toothless (tss), has been mapped to the same region and also have P-tagged mutants with the posterior eye pigmentation phenotype (Pradip Sinha, pers. comm.) Complementation tests showed that tss ...
... al. 1984; Salzberg et al. 1994, 1997). Thus, these mutations define the hth locus. Another lethal locus, toothless (tss), has been mapped to the same region and also have P-tagged mutants with the posterior eye pigmentation phenotype (Pradip Sinha, pers. comm.) Complementation tests showed that tss ...
Specificity of Insertion by the Translocatable Tetracycline Resistance Element Tn10.
... mapping places an upper limit of about 50 basepairs on the distance between different insertions within a cluster. Different insertions within a cluster usually have the same reversion frequency; however, heterogeneity in reversion frequency has been detected in at least two clusters. For most clust ...
... mapping places an upper limit of about 50 basepairs on the distance between different insertions within a cluster. Different insertions within a cluster usually have the same reversion frequency; however, heterogeneity in reversion frequency has been detected in at least two clusters. For most clust ...
New variants of the tet(M) gene in Clostridium
... other two isolates harbouring tet(M)1 for the hypothesized presence of two Tn916-like elements in the chromosome. However, the sequencing of the tet(M) region in these strains revealed that there was no superimposition of different PCR products, suggesting that if two elements were present both elem ...
... other two isolates harbouring tet(M)1 for the hypothesized presence of two Tn916-like elements in the chromosome. However, the sequencing of the tet(M) region in these strains revealed that there was no superimposition of different PCR products, suggesting that if two elements were present both elem ...
Characterization of an Immuno-dominant Variable Surface
... genomic AZAPII clones as described previously (41) . Phage DNA and plasmid DNA were purified by standard methods (42) . Sequence Analysis. With the exception of the first 207 bp, the entire sequence of gene M17 presented in Fig. 3 was determined on both strands in genomic clone pBSgM17-1 and on one ...
... genomic AZAPII clones as described previously (41) . Phage DNA and plasmid DNA were purified by standard methods (42) . Sequence Analysis. With the exception of the first 207 bp, the entire sequence of gene M17 presented in Fig. 3 was determined on both strands in genomic clone pBSgM17-1 and on one ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.