VCR 221 - Potato - UC Davis Plant Sciences
... Construction of a potato YAC library and identification of clones linked to the disease resistance loci R1 and Gro1. TAG95:954-960. Lentini, Z., E.D. Earle and R.L. Plaisted. 1990. Insect-resistant plants with improved horticultural traits from interspecific potato hybrids grown in vitro. TAG 80:95- ...
... Construction of a potato YAC library and identification of clones linked to the disease resistance loci R1 and Gro1. TAG95:954-960. Lentini, Z., E.D. Earle and R.L. Plaisted. 1990. Insect-resistant plants with improved horticultural traits from interspecific potato hybrids grown in vitro. TAG 80:95- ...
Comparative gene mapping in Arabidopsis lyrata chromosomes 6
... (a self-incompatible species whose total DNA content is about 60 % higher). Such data are of interest because it is predicted that inbreeders should have higher recombination rates than outcrossers (Charlesworth et al., 1977, 1979 ; Hedrick et al., 1978). The evidence to date is purely from chiasma ...
... (a self-incompatible species whose total DNA content is about 60 % higher). Such data are of interest because it is predicted that inbreeders should have higher recombination rates than outcrossers (Charlesworth et al., 1977, 1979 ; Hedrick et al., 1978). The evidence to date is purely from chiasma ...
The American University in Cairo School of Science and Engineering
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
uncorrected page proofs
... death occurs during early development and the pregnancy never proceeds to term. A pregnancy may still be carried to term if the chromosomal changes involve a few particular chromosomes (see table 14.2). The most common chromosomal anomaly seen in human populations is Down syndrome (DS), in which the ...
... death occurs during early development and the pregnancy never proceeds to term. A pregnancy may still be carried to term if the chromosomal changes involve a few particular chromosomes (see table 14.2). The most common chromosomal anomaly seen in human populations is Down syndrome (DS), in which the ...
Role of NAD+-Dependent Malate Dehydrogenase in the Metabolism
... the three domains of life. It plays crucial roles in many metabolic pathways, including the tricarboxylic acid (TCA) cycle, energy generation and the formation of metabolites for biosynthesis. Aerobic bacteria utilizing methane as a sole source of carbon and energy (methanotrophs) belong to the Alph ...
... the three domains of life. It plays crucial roles in many metabolic pathways, including the tricarboxylic acid (TCA) cycle, energy generation and the formation of metabolites for biosynthesis. Aerobic bacteria utilizing methane as a sole source of carbon and energy (methanotrophs) belong to the Alph ...
ppt for
... 2) Olfactory receptors and miRNAs are lost at the same rate with which they are acquired. However, not a single cluster loss was observed for piRNA. ...
... 2) Olfactory receptors and miRNAs are lost at the same rate with which they are acquired. However, not a single cluster loss was observed for piRNA. ...
... Acetoin is a four carbon acetohydroxy compound that is synthesized in all simple bacteria, plants and animals, as the by-product of branched chain amino acid synthesis. Organisms such as Klebsiella and Bacillus synthesize acetoin during late exponential phase of the growth by alsSD operon. The alsSD ...
A Selective Sweep Driven by Pyrimethamine Treatment in Southeast
... locations 2,000 km apart. Three features of these data are of especial interest. (1) Pyrimethamine resistance is generally assumed to have evolved multiple times because the genetic basis is simple and resistance can be selected easily in the laboratory. Yet our data clearly indicate a single origin ...
... locations 2,000 km apart. Three features of these data are of especial interest. (1) Pyrimethamine resistance is generally assumed to have evolved multiple times because the genetic basis is simple and resistance can be selected easily in the laboratory. Yet our data clearly indicate a single origin ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... from P. vulgaris is missing the N-terminal signal sequence [9]. Therefore two 5 end primers were designed so as to generate a full-length clone and a truncated version of the gene by omitting 72 bases encoding the signal sequence. In order to facilitate cloning into a pET-28a vector (Novagen), the ...
... from P. vulgaris is missing the N-terminal signal sequence [9]. Therefore two 5 end primers were designed so as to generate a full-length clone and a truncated version of the gene by omitting 72 bases encoding the signal sequence. In order to facilitate cloning into a pET-28a vector (Novagen), the ...
Analysis of the genetic potential of the spongederived fungus
... Isolation of chromosomal DNA from P. chrysogenum ............................................................ - 181 12.6 Determination of DNA concentration and purity .................................................................... - 181 12.7 Restriction enzyme digestion of DNA ................. ...
... Isolation of chromosomal DNA from P. chrysogenum ............................................................ - 181 12.6 Determination of DNA concentration and purity .................................................................... - 181 12.7 Restriction enzyme digestion of DNA ................. ...
Molecular evolutionary analysis of the American pika
... inhabiting alpine environments. For alpine mammals, metabolic heat production and cellular mechanisms to cope with hypoxia are critical for survival and reproduction. Thus, because of the pikas’ long association with alpine environments, they may have genetically adapted to alpine conditions. Here, ...
... inhabiting alpine environments. For alpine mammals, metabolic heat production and cellular mechanisms to cope with hypoxia are critical for survival and reproduction. Thus, because of the pikas’ long association with alpine environments, they may have genetically adapted to alpine conditions. Here, ...
Processing the RefSeq and CCDS Annotation Datasets Using the SAS System: Creation of Gene Reference
... of STRAND, either Lines 37-171 or Lines 174-331 are executed. The authors avoided using ELSE IF for two reasons: the computational require of two exclusive IF statements is negligible and indenting made the code more difficult to read on a smaller screen. In either set of IF-THEN-DO-END statements, ...
... of STRAND, either Lines 37-171 or Lines 174-331 are executed. The authors avoided using ELSE IF for two reasons: the computational require of two exclusive IF statements is negligible and indenting made the code more difficult to read on a smaller screen. In either set of IF-THEN-DO-END statements, ...
PDF
... and polarized light sensors (Hardie, 1985). According to their differentiated function, R1-6 innervate the first OL neuropil, which is the lamina, whereas R7 and R8 axons run across the lamina to innervate the neuropil beneath, which is the medulla. Next, the lobula and lobula plate (together called ...
... and polarized light sensors (Hardie, 1985). According to their differentiated function, R1-6 innervate the first OL neuropil, which is the lamina, whereas R7 and R8 axons run across the lamina to innervate the neuropil beneath, which is the medulla. Next, the lobula and lobula plate (together called ...
Careful risk assessment needed to evaluate transgenic fish
... donor DNA. Therefore, fish engineered with recombinant DNA derived entirely from fish are considered transgenic. The recombinant DNA sequence, or construct, is usually comprised of several different regions including a start signal or “promoter,” the coding region for the target protein, and a stop si ...
... donor DNA. Therefore, fish engineered with recombinant DNA derived entirely from fish are considered transgenic. The recombinant DNA sequence, or construct, is usually comprised of several different regions including a start signal or “promoter,” the coding region for the target protein, and a stop si ...
Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... sized by the modified triester method (Q; T or C, N; any base).17) One of these sequences ...
... sized by the modified triester method (Q; T or C, N; any base).17) One of these sequences ...
Genotype C of hepatitis B virus can be classified into at least two
... DNA polymerase (Applied Biosystems). The sequences of oligonucleotide primers and their combinations used in this study are listed in Table 1. To obtain the entire sequence, the first round of PCR was carried out for 40 cycles (98 uC for 10 s, 50 uC for 20 s and 72 uC for 2?5 min) followed by extens ...
... DNA polymerase (Applied Biosystems). The sequences of oligonucleotide primers and their combinations used in this study are listed in Table 1. To obtain the entire sequence, the first round of PCR was carried out for 40 cycles (98 uC for 10 s, 50 uC for 20 s and 72 uC for 2?5 min) followed by extens ...
Standard PDF - Wiley Online Library
... Determination of the number and identities of all proteins encoded in the P. carinii genome will require its sequencing. ...
... Determination of the number and identities of all proteins encoded in the P. carinii genome will require its sequencing. ...
Why are most organelle genomes transmitted maternally?
... to cells harboring spectinomycin-resistant paternal chloroplasts, whereas white sectors contain only cells with antibiotic-sensitive maternal plastids [79]. Diffuse areas of green tissue indicate incomplete sorting-out of maternal and paternal plastids (Box 1). B: Biparental chloroplast inheritance ...
... to cells harboring spectinomycin-resistant paternal chloroplasts, whereas white sectors contain only cells with antibiotic-sensitive maternal plastids [79]. Diffuse areas of green tissue indicate incomplete sorting-out of maternal and paternal plastids (Box 1). B: Biparental chloroplast inheritance ...
Lecture 4
... Flybase and Modern Drosophila Research 1. No longer have to clone and sequence a gene- reagents readily available. 2. Store of characterized mutants. RNAi 3. Large scale genome screens like 2 hybrid analysis and insitu hybridization are available. 4. Allows researchers to propose and ...
... Flybase and Modern Drosophila Research 1. No longer have to clone and sequence a gene- reagents readily available. 2. Store of characterized mutants. RNAi 3. Large scale genome screens like 2 hybrid analysis and insitu hybridization are available. 4. Allows researchers to propose and ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... primers are added to a mixture of template DNA, all four deoxynucelotide triphosphates (dNTPs), and a heat-stable DNA polymerase (other reagents may also be included). The mixture then undergoes several cycles comprised of three basic steps: strand separation (denaturing), primer hybridization (anne ...
... primers are added to a mixture of template DNA, all four deoxynucelotide triphosphates (dNTPs), and a heat-stable DNA polymerase (other reagents may also be included). The mixture then undergoes several cycles comprised of three basic steps: strand separation (denaturing), primer hybridization (anne ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.