Download Lab Quiz 4 Key

Biol 211, Fall 2008
Lab Quiz #4
Name ____________________
1. What was the purpose of using the PMM (Plant Master Mix) against all of the samples in the GMO
Investigator lab (e.g., what does it tell you about your data)? (0.5 pt)
{To determine whether or not you got DNA]
2. What is the function of PCR (polymerase chain reaction)? (0.5 pt)
[To produce many copies of DNA from only a small amount]
3. What specific information do you get about DNA by running it through electrophoresis? (0.5 pt)
[The length of the fragment]
4. What are GMO’s? (0.5 pt)
[Organism that has been genetically modified by having foreign DNA inserted into its genome]
5. What is a plasmid? (0.5 pt)
[small ring of DNA found in bacteria that is separate from the main chromosome and that carry
accessory genes (e.g., genes for antibiotic resistance and metabolic pathways)]
6. In the bacterial transformation lab, what were the dependent variables? (0.5 pt)
[growth of colonies and whether the bacteria glow or not]
7. Why is arabinose added to one of the petri dishes in the bacterial transformation lab? (0.5 pt)
[the GFP gene will only get switched on as part of a metabolic sequence that processes the
sugar arabinose. In order for the metabolic pathway to be switched on, arabinose must be present (acts
as inducer)]
8. What is the purpose of the heat shock treatment in the bacterial transformation lab? (0.5 pt)
[to induce the bacteria to take up the plasmid]
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