Download Protein - Geneaid

Protein
Prestained Protein Ladder V..................................................................81
Prestained Protein Ladder 245 kDa.......................................................81
Protein Loading D ye..............................................................................82
R everse Protein Stain K it.......................................................................82
Protein
Prestained Protein Ladder V Introduction
The Prestained Protein Ladder V is a three-color prestained protein standard
which includes protein dye for convenient protein sample staining. The
protein ladder consists of 10 prestained proteins covering a wide range of
molecular weights (10 to 180 kDa). Proteins are covalently coupled with a
blue chromophore except for two reference bands (one green and one red
band) when separated on SDS-PAGE. The ladder is supplied in a gel loading
buffer and is ready to use, without heating, diluting, or a reducing agent prior
to loading.
Advantages
• Wide Molecular Weight range: 10-180 kDa
• Supplied buffer for direct gel loading
• Sharp bands with 28 and 75 KDa reference bands (green/red dye)
• Includes Protein Loading Dye (2 ml, 5X)
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on
15-well or 10-well mini-gel, respectively
• 2~3 μl per well for general Western transferring
• Storage: 25ºC for 2 weeks, 4ºC for 3 months, -20ºC for 24 months
Prestained Protein Ladder V Molecular Weight Estimation
Applications
Monitor protein migration/sample in SDS-PAGE, Monitor protein transfer onto
membranes during Western Blotting, Sizing of proteins on SDS-PAGE and
Western blots
Components
Approximately 0.2~0.4 mg/ml of each protein in buffer {20 mM Trisphosphate
pH7.5 at 25°C, 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v)
glycerol}, Protein Loading Dye (2 ml, 5X)
Figure 1. Migration patterns of various electrophoretic conditions according to type
and percentage of running buffer.
Prestained Protein Ladder 245 kDa Introduction
Prestained Protein Ladder 245 kDa Molecular Weight Estimation
Prestained Protein Ladder (245 kDa) is a three-color protein standard with 12
pre-stained proteins covering a wide range of molecular weights for 10 to 245
kDa. Proteins are covalently coupled with a blue chromophore except for two
reference bands (one green and one red band) when separated on
SDS-PAGE (Tris-glycine buffer). The ladder is supplied in a gel loading buffer
and is ready to use, without requiring heating, diluting, or a reducing agent
prior to loading.
Advantages
• Wide Molecular Weight range: 10-245 kDa
• Supplied buffer for direct gel loading
• Sharp bands with 28 and 75 KDa reference bands (green/red dye)
• Includes Protein Loading Dye (2 ml, 5X)
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on
15-well or 10-well mini-gel, respectively
• 2~3 μl per well for general Western transferring
• Storage: 25ºC for 2 weeks, 4ºC for 3 months, -20ºC for 24 months
Applications
Monitor protein migration/sample in SDS-PAGE, protein transfer onto
membranes during Western Blotting, sizing of proteins on SDS-PAGE and
Western blots
Figure 2. Migration patterns of various electrophoretic conditions according to type
and percentage of running buffer.
Components
Approximately 0.2~0.4 mg/ml of each protein in buffer {20 mM Trisphosphate
pH7.5 at 25°C, 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v)
glycerol}, Protein Loading Dye (2 ml, 5X)
Page 81
www.geneaid.com
Protein
Protein
Protein Loading Dye Introduction
Geneaid's ready-to-use (5X) Protein Loading Dye is ideal for preparing
protein samples to be separated in SDS-polyacrylamide gel electrophoresis
(SDS-PAGE) and allows for easy sample monitoring during electrophoresis.
Protein Loading Dye is conveniently included with Geneaid's Prestained
Protein Ladder V.
Advantages
• Conveniently included with Geneaid's Prestained Protein Ladder V
• Ready-to-use solution
• Ideal for preparing protein samples to be separated in SDS-polyacrylamide
gel electrophoresis (SDS-PAGE)
• Allows for easy sample monitoring during electrophoresis
• Storage: 25ºC for 2 weeks, 4ºC for 3 months, -20ºC for 24 months
Applications
Preparing of protein samples to be separated in SDS-polyacrylamide gel
electrophoresis
(SDS-PAGE),
Easy
sample
monitoring
during
electrophoresis
Reverse Protein Stain Kit Introduction
The Reverse Protein Stain Kit uses imidazole and zinc salts for protein
detection as low as 1 ng in electrophoresis gels. The method is based on
selective precipitation of a white imidazole–zinc complex in the gel, except in
zones where proteins are located which remain transparent. When the gel is
placed on a dark background, the negative gel image can be converted to a
positive image with black bands, spots, and a white background. This stain is
as sensitive as most silver stains and requires only 10 minutes to complete.
Fixation of proteins to the gel is not needed, there is no interaction of the stain
with the protein, and complete destaining of the matrix can be achieved.
Imidazole-zinc reverse stain uses only two staining solutions, which can be
easily diluted from stock solutions. For the imidazole-zinc negative staining,
no fixation is necessary. Gels are typically stained immediately after
electrophoresis.
Advantages
• Protein detection as low as 1 ng in electrophoresis gels
• Fixation of proteins to the gel is not needed so will not affect protein samples
• Complete destaining of the matrix can be achieved
• Rapid Staining: <10 minutes compared to conventional staining methods
requiring up to 24 hours
• Non toxic (no organic solvents)
• Alternative staining methods can be used direclty following this procedure
• Storage: room temperature for up to 2 years
Applications
Imidazole-zinc reverse stain is fully compatible to down-stream applications,
such as Mass spectrometry, Edman sequencing, Electroelution, and
Membrane blotting techniques
Reverse Protein Stain Kit <10 Minute Gel
Figure 1. The Reverse Protein
Stain Kit uses imidazole and zinc
salts for protein detection as low
as 1 ng in electrophoresis gels.
The method is based on selective
precipitation
of
a
white
imidazole–zinc complex in the
gel, except in those zones where
proteins are located.
Components
• Protein Stain Buffer I and II
• Black Stain Box
Protein
www.geneaid.com
Page 82