Academic paper: The free-living amoeba Willaertia magna, is
... 1997; Neumeister et al., 1997; Abu Kwaik et al., 1998a; Harb et al., 1998). Recently, it was shown that Dictyostelium discoideum is also able to support the bacterial growth and proliferation (Hagele et al., 2000; Solomon et al., 2000). Our results with A. castellanii and H. vermiformis are in agree ...
... 1997; Neumeister et al., 1997; Abu Kwaik et al., 1998a; Harb et al., 1998). Recently, it was shown that Dictyostelium discoideum is also able to support the bacterial growth and proliferation (Hagele et al., 2000; Solomon et al., 2000). Our results with A. castellanii and H. vermiformis are in agree ...
Microfluidic-Based Multi-Organ Platforms for Drug Discovery
... models and organoid systems [13–15]. This technology has also been utilized to develop integrated multi-organ platforms as an essential testing requirement during the advanced drug discovery steps. Nevertheless, there are major challenges hindering the development of predictive multi-organ models an ...
... models and organoid systems [13–15]. This technology has also been utilized to develop integrated multi-organ platforms as an essential testing requirement during the advanced drug discovery steps. Nevertheless, there are major challenges hindering the development of predictive multi-organ models an ...
Stochastic and reversible aggregation of mRNA with expanded CUG
... To analyze the dynamics of the CUG-rich RNA foci in living cells, the lacZ–MS2–5CUG and lacZ–MS2–145CUG cell lines were infected with retroviral vector expressing MS2–GFP, and live-cell microscopy was performed at a rate of three images per second using a spinning disk confocal microscope, to visual ...
... To analyze the dynamics of the CUG-rich RNA foci in living cells, the lacZ–MS2–5CUG and lacZ–MS2–145CUG cell lines were infected with retroviral vector expressing MS2–GFP, and live-cell microscopy was performed at a rate of three images per second using a spinning disk confocal microscope, to visual ...
Selective and differential interactions of BNN27
... neurons in vitro, ex vivo and in vivo in NGF null mice, 2) to test its potency to intervene in the neuronal pathways conveying hyperalgesia in vivo, 3) to define the ability of BNN27 to interact with and activate TrkA receptors and the exact post-receptor cellular mechanisms by which it affects NGF-s ...
... neurons in vitro, ex vivo and in vivo in NGF null mice, 2) to test its potency to intervene in the neuronal pathways conveying hyperalgesia in vivo, 3) to define the ability of BNN27 to interact with and activate TrkA receptors and the exact post-receptor cellular mechanisms by which it affects NGF-s ...
Evasion of the Immune Response by Trypanosoma cruzi during
... that affects millions of people mainly in Latin America. To establish a life-long infection, T. cruzi must subvert the vertebrate host’s immune system, using strategies that can be traced to the parasite’s life cycle. Once inside the vertebrate host, metacyclic trypomastigotes rapidly invade a wide ...
... that affects millions of people mainly in Latin America. To establish a life-long infection, T. cruzi must subvert the vertebrate host’s immune system, using strategies that can be traced to the parasite’s life cycle. Once inside the vertebrate host, metacyclic trypomastigotes rapidly invade a wide ...
Evidence for chloroplast control of external Ca -induced
... Figure 1(d), 2 mM CaCl2 induced stomatal closure within 2 h in wild-type Arabidopsis. On the other hand, [Ca2+]extinduced stomatal closure was completely impaired in cas-1 (Figure 1d) and cas-2 (Figure S3) mutant alleles. This is consistent with a previous report that [Ca2+]ext-induced stomatal clos ...
... Figure 1(d), 2 mM CaCl2 induced stomatal closure within 2 h in wild-type Arabidopsis. On the other hand, [Ca2+]extinduced stomatal closure was completely impaired in cas-1 (Figure 1d) and cas-2 (Figure S3) mutant alleles. This is consistent with a previous report that [Ca2+]ext-induced stomatal clos ...
Exosomes: Implications in HIV-1 Pathogenesis
... budding of enveloped viruses [59–61], cytokinesis, and membrane repair, as well as the role of RAB proteins in secretion of other soluble factors; inhibition of ESCRT and RAB pathways may modulate host cellular functions unrelated to exosome formation/secretion or may alter the cargo composition of ...
... budding of enveloped viruses [59–61], cytokinesis, and membrane repair, as well as the role of RAB proteins in secretion of other soluble factors; inhibition of ESCRT and RAB pathways may modulate host cellular functions unrelated to exosome formation/secretion or may alter the cargo composition of ...
Graded maternal short gastrulation protein contributes to embryonic
... morphology of follicle cells, which is clearly distinct in dorsal and ventral regions at stage 10B. Double stains for anti-Sog 8A and anti-Grk antibody were also performed to unambiguously orient staining within the egg chamber. Since images for Sog single stains were of better quality, however, tho ...
... morphology of follicle cells, which is clearly distinct in dorsal and ventral regions at stage 10B. Double stains for anti-Sog 8A and anti-Grk antibody were also performed to unambiguously orient staining within the egg chamber. Since images for Sog single stains were of better quality, however, tho ...
Compare TIRF Geometries
... TIRF is a powerful analytical technique with numerous applications in the area of life sciences [1-3]. In particular, TIRF is “...a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made ...
... TIRF is a powerful analytical technique with numerous applications in the area of life sciences [1-3]. In particular, TIRF is “...a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made ...
Introduction Wnt signaling in development
... sex chromosomes and have a specialized tail structure that allows them to mate with hermaphrodites (Hodgkin, 1983). This makes it possible to perform crosses, for example to combine different mutations or to genetically map mutations in positional cloning approaches. Traditionally, mutations have be ...
... sex chromosomes and have a specialized tail structure that allows them to mate with hermaphrodites (Hodgkin, 1983). This makes it possible to perform crosses, for example to combine different mutations or to genetically map mutations in positional cloning approaches. Traditionally, mutations have be ...
The Role Of The Planar Cell Polarity Pathway In The Second Heart
... The heart of creatures is the foundation of life, the Prince of all, the sun of their microcosm, from where all vigor and strength does flow. —William Harvey, De Motu Cordis, 1628 ...
... The heart of creatures is the foundation of life, the Prince of all, the sun of their microcosm, from where all vigor and strength does flow. —William Harvey, De Motu Cordis, 1628 ...
Formation and excretion of autophagic plastids (plastolysomes) in
... isolation of this particular developmental stage is essential for a successful induction, since it is known that this is the stage where the microspore is most sensitive to reprogramming treatments (Maraschin et al., 2005; Seguí-Simarro and Nuez, 2008; Dunwell, 2010). Just after the isolation, cultu ...
... isolation of this particular developmental stage is essential for a successful induction, since it is known that this is the stage where the microspore is most sensitive to reprogramming treatments (Maraschin et al., 2005; Seguí-Simarro and Nuez, 2008; Dunwell, 2010). Just after the isolation, cultu ...
interactions between the glycosylated gag protein of a murine
... newly synthesized proviral DNA. Other deaminase-independent mechanisms of inhibition have been identified, such as directly inhibiting reverse transcription. Both HIV and murine leukemia viruses (MuLVs) have evolved mechanisms to evade the action of the APOBEC proteins. HIV encodes the Vif protein w ...
... newly synthesized proviral DNA. Other deaminase-independent mechanisms of inhibition have been identified, such as directly inhibiting reverse transcription. Both HIV and murine leukemia viruses (MuLVs) have evolved mechanisms to evade the action of the APOBEC proteins. HIV encodes the Vif protein w ...
Type XIII collagen. Structural and functional characterization of the
... Hägg, M. D., Ph. D., Ari-Pekka Kvist, Ph. D., and Malin Sund, M. D., Ph. D. are warmly acknowledged for their contributions to exploring the importance of the type XIII collagen, which formed the essential basis of the work presented in this thesis. I also want to express my sincere appreciation to ...
... Hägg, M. D., Ph. D., Ari-Pekka Kvist, Ph. D., and Malin Sund, M. D., Ph. D. are warmly acknowledged for their contributions to exploring the importance of the type XIII collagen, which formed the essential basis of the work presented in this thesis. I also want to express my sincere appreciation to ...
R.W. Moreadith, T.X. Viegas, M.D. Bentley, J.M. Harris, Z. Fang, K
... result the polymer influences the properties of the newly conjugated molecule. They have also been used to enable other types of drug delivery systems such as liposomes, micelles and dendrimers where they provide stealth-like properties. One of the first studies with drug conjugation was reported by ...
... result the polymer influences the properties of the newly conjugated molecule. They have also been used to enable other types of drug delivery systems such as liposomes, micelles and dendrimers where they provide stealth-like properties. One of the first studies with drug conjugation was reported by ...
Clinical Medicine Reviews in Therapeutics Intermittent Asthma
... acute exacerbations and bronchoconstriction are often followed by lengthy symptom-free periods which persist for several weeks, or even months. The exacerbations are often severe, being triggered by viral infections, exercise, or exposure to allergens, resulting in bronchoconstriction, either in ...
... acute exacerbations and bronchoconstriction are often followed by lengthy symptom-free periods which persist for several weeks, or even months. The exacerbations are often severe, being triggered by viral infections, exercise, or exposure to allergens, resulting in bronchoconstriction, either in ...
Benzylpenicillin inhibits the renal excretion of acyclovir by OAT1 and
... infections. Approximately 83% of acyclovir is excreted by kidney in its unchanged forms [13]. Renal excretion of acyclovir is reduced by probenecid, a typical inhibitor of OAT [13]. Although neither possesses a typical anionic moiety, the results suggest that the OAT system is responsible for the tu ...
... infections. Approximately 83% of acyclovir is excreted by kidney in its unchanged forms [13]. Renal excretion of acyclovir is reduced by probenecid, a typical inhibitor of OAT [13]. Although neither possesses a typical anionic moiety, the results suggest that the OAT system is responsible for the tu ...
PDF + SI - The Journal of Immunology
... cytokines, such as IL-12, TNF-a, and IFN-g (5), that control inflammatory responses. IL-10 promotes the development of regulatory T cells for the control of excessive immune responses (6). In contrast, IL-12 is an important mediator of cell-mediated immunity and is expected to augment the natural im ...
... cytokines, such as IL-12, TNF-a, and IFN-g (5), that control inflammatory responses. IL-10 promotes the development of regulatory T cells for the control of excessive immune responses (6). In contrast, IL-12 is an important mediator of cell-mediated immunity and is expected to augment the natural im ...
Increased Levels of LPS-Binding Protein in Bovine - PubAg
... dissolved in 10 ml of 0.85% saline solution and sterile filtered. The LPS used in the present study was a highly purified preparation that was prepared by phenol extraction and further purified by ion-exchange chromatography. We have previously established that the bioactivity of this preparation is ...
... dissolved in 10 ml of 0.85% saline solution and sterile filtered. The LPS used in the present study was a highly purified preparation that was prepared by phenol extraction and further purified by ion-exchange chromatography. We have previously established that the bioactivity of this preparation is ...
Mitochondrial Dynamics
... localizes to the inner surface of the cell membrane at division sites, where it forms a ring structure (Z ring) that enables constriction and scission of the parent into two daughter cells. FtsZ is a GTPase, which can hydrolyze guanosine triphosphate to provide a source of energy. However, it is tho ...
... localizes to the inner surface of the cell membrane at division sites, where it forms a ring structure (Z ring) that enables constriction and scission of the parent into two daughter cells. FtsZ is a GTPase, which can hydrolyze guanosine triphosphate to provide a source of energy. However, it is tho ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.