Actin-Dependent and -Independent Functions of
... microtubules (and not other types of microtubule configurations, such as endoplasmic microtubules) are the basis of branching in trichomes, we analyzed the branching process at increased spatiotemporal resolution. Analyses were performed for the wild type and for overbranched tor2. This was done by c ...
... microtubules (and not other types of microtubule configurations, such as endoplasmic microtubules) are the basis of branching in trichomes, we analyzed the branching process at increased spatiotemporal resolution. Analyses were performed for the wild type and for overbranched tor2. This was done by c ...
Nasal Drug Delivery
... nasal epithelial biopsies using the Western Blot technique. The results demonstrated that the Ussing chamber model using porcine nasal mucosa has potential as a tool for evaluating mechanisms of nasal absorption and predicting the in vivo effects of absorption enhancers. Moreover, porcine nasal muco ...
... nasal epithelial biopsies using the Western Blot technique. The results demonstrated that the Ussing chamber model using porcine nasal mucosa has potential as a tool for evaluating mechanisms of nasal absorption and predicting the in vivo effects of absorption enhancers. Moreover, porcine nasal muco ...
Review The cellular functions of clathrin
... of direct proof that CCSs were actually CCVs that contained cargo. A recent study demonstrated the uptake of fluorescently-tagged cargo (LDL, transferrin and reovirus particles) in relation to clathrin using dual-colour confocal imaging [54]. They found that a quarter of CCSs disassembled without in ...
... of direct proof that CCSs were actually CCVs that contained cargo. A recent study demonstrated the uptake of fluorescently-tagged cargo (LDL, transferrin and reovirus particles) in relation to clathrin using dual-colour confocal imaging [54]. They found that a quarter of CCSs disassembled without in ...
Glutamate 83 and arginine 85 of helix H3 bend are key residues for
... The FtsZ ring is formed in the middle of the cell and is attached to the cytoplasmic membrane through ZipA [2]. In order to evaluate the effect of these mutations on cell morphology and FtsZ localization, VIP5 cells in exponential phase of growth carrying plasmids with FtsZ (WT), FtsZ(E83Q), and Fts ...
... The FtsZ ring is formed in the middle of the cell and is attached to the cytoplasmic membrane through ZipA [2]. In order to evaluate the effect of these mutations on cell morphology and FtsZ localization, VIP5 cells in exponential phase of growth carrying plasmids with FtsZ (WT), FtsZ(E83Q), and Fts ...
Airway surface liquid pH in well-differentiated airway - AJP-Cell
... below. Arterial blood (0.2–0.3 ml) was sampled through a PE-10 catheter inserted into the carotid artery, and blood pH and PCO2 were measured using a blood gas analyzer (Ciba Corning Diagnostic). After completion of the measurements, mice were euthanized by an overdose of pentobarbital (150 mg/kg). ...
... below. Arterial blood (0.2–0.3 ml) was sampled through a PE-10 catheter inserted into the carotid artery, and blood pH and PCO2 were measured using a blood gas analyzer (Ciba Corning Diagnostic). After completion of the measurements, mice were euthanized by an overdose of pentobarbital (150 mg/kg). ...
Guideline for the Quality, Safety, and Efficacy Assurance of Follow
... Since many glycoprotein products have significant heterogeneity at each glycosylation site, it is often difficult to demonstrate comparability based on the structural analysis alone. In addition, even if glycoprotein products are produced using the same host cell, the heterogeneity of glycosylation ...
... Since many glycoprotein products have significant heterogeneity at each glycosylation site, it is often difficult to demonstrate comparability based on the structural analysis alone. In addition, even if glycoprotein products are produced using the same host cell, the heterogeneity of glycosylation ...
DIURETICS
... as they may cause EC volume expansion, overload of the heart, and thereby, pulmonary edema. Urea is contraindicated in hepatic cirrhosis. At high concentration, urea may inhibit arginase and thereby it impairs the elimination of NH3 in the urea cycle. Glycerin is contraindicated in diabetes mell ...
... as they may cause EC volume expansion, overload of the heart, and thereby, pulmonary edema. Urea is contraindicated in hepatic cirrhosis. At high concentration, urea may inhibit arginase and thereby it impairs the elimination of NH3 in the urea cycle. Glycerin is contraindicated in diabetes mell ...
Class II Histone Deacetylases Are Directly Recruited by BCL6
... deacetylases, HDAC11 and HDAC2 (13-20). However, these co-repressors do not explain all the regulatory properties of the POK proteins. In fact, it appears that POK transcriptional repressors are capable of recruiting other co-repressors. Indeed, C-terminal binding protein might interact with Drosoph ...
... deacetylases, HDAC11 and HDAC2 (13-20). However, these co-repressors do not explain all the regulatory properties of the POK proteins. In fact, it appears that POK transcriptional repressors are capable of recruiting other co-repressors. Indeed, C-terminal binding protein might interact with Drosoph ...
Cellulose Biosynthesis: Current Views and
... crystalline sub-allomorphs of cellulose I, namely Ia and Ib are found to occur in the cellulose obtained from natural sources (Attala and VanderHart, 1984). Cellulose Ia and cellulose Ib differ with respect to their crystal packing, molecular conformation and hydrogen bonding and these differences m ...
... crystalline sub-allomorphs of cellulose I, namely Ia and Ib are found to occur in the cellulose obtained from natural sources (Attala and VanderHart, 1984). Cellulose Ia and cellulose Ib differ with respect to their crystal packing, molecular conformation and hydrogen bonding and these differences m ...
Vesicles versus Tubes: is ER-Golgi Transport in
... features, especially when compared with its mammalian counterpart. It is ...
... features, especially when compared with its mammalian counterpart. It is ...
Direct Interaction between Survivin and Smac/DIABLO Is Essential
... of cytochrome c into the cytosol from the mitochondrial intermembrane space (33). To further investigate whether the mitochondria are involved in this event, Taxol-treated HeLa cells were first lysed and divided into membrane pellet and cytosolic fraction. The pellet was then treated with 0.5% bile ...
... of cytochrome c into the cytosol from the mitochondrial intermembrane space (33). To further investigate whether the mitochondria are involved in this event, Taxol-treated HeLa cells were first lysed and divided into membrane pellet and cytosolic fraction. The pellet was then treated with 0.5% bile ...
Chitin and Chitosan in Fungi - Wiley-VCH
... (Saxena and Brown, 1997). Alignment with the deduced protein sequences of most known chitin synthase genes (CHS ) reveals five to seven conserved domains (Xoconostle-Cazares et al., 1996). CS is detected in membrane fractions and in chitosomes, the latter constituting small secretory vesicles (ca. 1 ...
... (Saxena and Brown, 1997). Alignment with the deduced protein sequences of most known chitin synthase genes (CHS ) reveals five to seven conserved domains (Xoconostle-Cazares et al., 1996). CS is detected in membrane fractions and in chitosomes, the latter constituting small secretory vesicles (ca. 1 ...
Myosin XIK is a major player in cytoplasm dynamics and is regulated
... emerged when RabD1 and RabC2a were found to interact with the tail of MYA2 in yeast two-hybrid and pull-down assays (Hashimoto et al., 2008). The localization of RabC2a to peroxisomes suggested that these small GTPases can mediate and regulate the interaction of MYA2 and its cargo organelles (Hashim ...
... emerged when RabD1 and RabC2a were found to interact with the tail of MYA2 in yeast two-hybrid and pull-down assays (Hashimoto et al., 2008). The localization of RabC2a to peroxisomes suggested that these small GTPases can mediate and regulate the interaction of MYA2 and its cargo organelles (Hashim ...
PDF
... Although autophagy is most evident following starvation, a basal level of constitutive autophagy appears to be a universal feature of nearly all eukaryotic cells. One important function of basal autophagy is to rid the cell of defective or superfluous organelles, and autophagy would appear to be the ...
... Although autophagy is most evident following starvation, a basal level of constitutive autophagy appears to be a universal feature of nearly all eukaryotic cells. One important function of basal autophagy is to rid the cell of defective or superfluous organelles, and autophagy would appear to be the ...
Chapter 1: Overview - Worcester Polytechnic Institute
... will work hard to make you guys proud. Los quiero muchisimo! To my sister, Claudia (marisca), for her continuous support and for keeping me motivated. I love being your big sister. To my brothers Eduardo and Hernán, although you two were far away from home during the last few years, our phone conver ...
... will work hard to make you guys proud. Los quiero muchisimo! To my sister, Claudia (marisca), for her continuous support and for keeping me motivated. I love being your big sister. To my brothers Eduardo and Hernán, although you two were far away from home during the last few years, our phone conver ...
FULL PRESCRIBING INFORMATION
... The effectiveness of dexmethylphenidate hydrochloride extended-release capsules in the treatment of ADHD in patients aged 6 years and older was established in two placebo-controlled studies in patients meeting DSM-IV criteria for ADHD [see Clinical Studies (14)]. A diagnosis of Attention Deficit Hyp ...
... The effectiveness of dexmethylphenidate hydrochloride extended-release capsules in the treatment of ADHD in patients aged 6 years and older was established in two placebo-controlled studies in patients meeting DSM-IV criteria for ADHD [see Clinical Studies (14)]. A diagnosis of Attention Deficit Hyp ...
Evidence for an apical Na–Cl cotransporter involved in ion uptake in
... Cation–chloride cotransporters, such as the Na+/K+/2Cl– cotransporter (NKCC) and Na+/Cl– cotransporter (NCC), are localized to the apical or basolateral plasma membranes of epithelial cells and are involved in active ion absorption or secretion. The objectives of this study were to clone and identif ...
... Cation–chloride cotransporters, such as the Na+/K+/2Cl– cotransporter (NKCC) and Na+/Cl– cotransporter (NCC), are localized to the apical or basolateral plasma membranes of epithelial cells and are involved in active ion absorption or secretion. The objectives of this study were to clone and identif ...
Fibronectin and Other Adhesive Glycoproteins
... The FN1 module is found only in chordates (Tucker and Chiquet-Ehrismann 2009) (see Chap. 1). It has been noted that the N-terminal sub-domain of the VWF type C module of a2 procollagen shows a structural similarity with the fibronectin FN1 module (O’Leary et al. 2004) and suggested that the VWF type ...
... The FN1 module is found only in chordates (Tucker and Chiquet-Ehrismann 2009) (see Chap. 1). It has been noted that the N-terminal sub-domain of the VWF type C module of a2 procollagen shows a structural similarity with the fibronectin FN1 module (O’Leary et al. 2004) and suggested that the VWF type ...
Roles of maltodextrin and glycogen phosphorylases in maltose
... transporter, respectively (Schönert et al., 2006). The maltose 6-phosphate derived from the uptake of maltose by the PTS is hydrolysed by a maltose-6-phosphate hydrolase, GlvA, resulting in the formation of glucose 6phosphate and glucose (Thompson et al., 1998). Maltodextrins are degraded by the co ...
... transporter, respectively (Schönert et al., 2006). The maltose 6-phosphate derived from the uptake of maltose by the PTS is hydrolysed by a maltose-6-phosphate hydrolase, GlvA, resulting in the formation of glucose 6phosphate and glucose (Thompson et al., 1998). Maltodextrins are degraded by the co ...
Depletion of intracellular Ca2+ stores enhances flow
... Measurement of [Ca2 þ ]i and store Ca2 þ content in endothelial cells An artery was placed in a specially designed flow chamber, which was mounted on an inverted microscope equipped with a 20 Olympus water immersion objective. The artery was pressurized to 50 mmHg and stabilized for 30 min in Tyro ...
... Measurement of [Ca2 þ ]i and store Ca2 þ content in endothelial cells An artery was placed in a specially designed flow chamber, which was mounted on an inverted microscope equipped with a 20 Olympus water immersion objective. The artery was pressurized to 50 mmHg and stabilized for 30 min in Tyro ...
FOCAL ADHESION KINASE: IN COMMAND AND CONTROL OF
... be connected to alterations in the polymerization or stabilization of actin and microtubule filaments. Additionally, because migrating cells experience changes in forces through integrin contacts that link the ECM with the cytoskeleton, FAK is important in the ‘sensing’ of mechanical forces that are ...
... be connected to alterations in the polymerization or stabilization of actin and microtubule filaments. Additionally, because migrating cells experience changes in forces through integrin contacts that link the ECM with the cytoskeleton, FAK is important in the ‘sensing’ of mechanical forces that are ...
Murine Regulatory T Cells Contain Hyperproliferative and Death
... than Tcon, particularly when the stimulation was weak (Fig. 1D). Differential sensitivity to activation-induced death between proliferating Treg and Tcon The differences of Treg proliferation between total Treg and live Treg prompted us to examine survival and death of Treg during in vitro culture. ...
... than Tcon, particularly when the stimulation was weak (Fig. 1D). Differential sensitivity to activation-induced death between proliferating Treg and Tcon The differences of Treg proliferation between total Treg and live Treg prompted us to examine survival and death of Treg during in vitro culture. ...
Apical-basal patterning in Arabidopsis - Development
... originates from descendants of both the apical and the basal daughter cell of the zygote. We have isolated a mutant of a new gene named BODENLOS (BDL) in which the primary root meristem is not formed whereas postembryonic roots develop and bdl seedlings give rise to fertile adult plants. Some bdl se ...
... originates from descendants of both the apical and the basal daughter cell of the zygote. We have isolated a mutant of a new gene named BODENLOS (BDL) in which the primary root meristem is not formed whereas postembryonic roots develop and bdl seedlings give rise to fertile adult plants. Some bdl se ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.