Altered morphology produced by ftsZ expression in
... Corynebacterium glutamicum is a Gram-positive bacterium that lacks the cell division FtsA protein and actin-like MreB proteins responsible for determining cylindrical cell shape. When the cell division ftsZ gene from C. glutamicum (ftsZCg) was cloned in different multicopy plasmids, the resulting co ...
... Corynebacterium glutamicum is a Gram-positive bacterium that lacks the cell division FtsA protein and actin-like MreB proteins responsible for determining cylindrical cell shape. When the cell division ftsZ gene from C. glutamicum (ftsZCg) was cloned in different multicopy plasmids, the resulting co ...
Mitochondrial dynamics, mitophagy and cardiovascular disease
... mitochondrial internal membrane, where it is cleaved by the protease PARL. Although Parkin-induced mitophagy has been shown to be dependent on the activity of PINK1, this process depends on DRP1-mediated mitochondrial fission (Tanaka et al. 2010; Fischer et al. 2012). A full understanding of the reg ...
... mitochondrial internal membrane, where it is cleaved by the protease PARL. Although Parkin-induced mitophagy has been shown to be dependent on the activity of PINK1, this process depends on DRP1-mediated mitochondrial fission (Tanaka et al. 2010; Fischer et al. 2012). A full understanding of the reg ...
Low peak bone mass and attenuated anabolic response to
... entire Cx43 reading frame with the lacZ reporter cassette selectively in bone-forming cells (Castro et al., 2003). With this model, Cre is expressed just before birth and in cells that are already partially differentiated into osteoblasts, thus providing an osteoblast-specific and postnatal gene abl ...
... entire Cx43 reading frame with the lacZ reporter cassette selectively in bone-forming cells (Castro et al., 2003). With this model, Cre is expressed just before birth and in cells that are already partially differentiated into osteoblasts, thus providing an osteoblast-specific and postnatal gene abl ...
Low peak bone mass and attenuated anabolic response to
... entire Cx43 reading frame with the lacZ reporter cassette selectively in bone-forming cells (Castro et al., 2003). With this model, Cre is expressed just before birth and in cells that are already partially differentiated into osteoblasts, thus providing an osteoblast-specific and postnatal gene abl ...
... entire Cx43 reading frame with the lacZ reporter cassette selectively in bone-forming cells (Castro et al., 2003). With this model, Cre is expressed just before birth and in cells that are already partially differentiated into osteoblasts, thus providing an osteoblast-specific and postnatal gene abl ...
Utilization and Transport of Mannitol in Olea
... showed that mannitol-grown cells remained viable 24 h after a 250 and 500 mM NaCl pulse, whereas extensive loss of cell viability was observed in sucrose-grown cells. OeMaT1 transcripts increased throughout maturation of olive fruits, suggesting that an OeMaT is involved in the accumulation of manni ...
... showed that mannitol-grown cells remained viable 24 h after a 250 and 500 mM NaCl pulse, whereas extensive loss of cell viability was observed in sucrose-grown cells. OeMaT1 transcripts increased throughout maturation of olive fruits, suggesting that an OeMaT is involved in the accumulation of manni ...
Thalidomide and its Analogs: A Potential Immunomodulatory
... pharmacological effects of Tha; additionally, Tha analogs do not compete for that binding site (Turk et al., 1996; Niwayama et al., 1998). α1-AGP is one of the major acute phase proteins in humans, rats, mice and other species; its concentration is elevated in response to systemic tissue injury, inf ...
... pharmacological effects of Tha; additionally, Tha analogs do not compete for that binding site (Turk et al., 1996; Niwayama et al., 1998). α1-AGP is one of the major acute phase proteins in humans, rats, mice and other species; its concentration is elevated in response to systemic tissue injury, inf ...
Bioconjug. Chem. 2013, DOI: 10.1021/bc400032x
... of numerous antiviral compounds. This polymer was chosen since polycarbonates possess biodegradability, low toxicity, and tunable mechanical properties. Furthermore, they degrade into carbon dioxide and alcohol, which unlike the degradation products of more frequently used polymers like poly(L-lacti ...
... of numerous antiviral compounds. This polymer was chosen since polycarbonates possess biodegradability, low toxicity, and tunable mechanical properties. Furthermore, they degrade into carbon dioxide and alcohol, which unlike the degradation products of more frequently used polymers like poly(L-lacti ...
Structure and function of the notochord: an essential
... become ossified in a fashion similar to cartilage. Consistent with this view, in mutant mice that lack type II collagen, the notochord is not replaced by bone, presumably because the type II collagen network is required for proper deposition of type X collagen. Evolutionary origins of the notochord ...
... become ossified in a fashion similar to cartilage. Consistent with this view, in mutant mice that lack type II collagen, the notochord is not replaced by bone, presumably because the type II collagen network is required for proper deposition of type X collagen. Evolutionary origins of the notochord ...
Response to Bacteria Excess Production of IL-12 and IL
... Disorders in enteric bacteria recognition by intestinal macrophages (M) are strongly correlated with the pathogenesis of chronic colitis; however the precise mechanisms remain unclear. The aim of the current study was to elucidate the roles of M in intestinal inflammation by using an IL-10-deficie ...
... Disorders in enteric bacteria recognition by intestinal macrophages (M) are strongly correlated with the pathogenesis of chronic colitis; however the precise mechanisms remain unclear. The aim of the current study was to elucidate the roles of M in intestinal inflammation by using an IL-10-deficie ...
Inositol trisphosphate and calcium oscillations
... Luminal loading Ca2+ oscillation model The main feature of the luminal loading Ca2+ oscillation model is that the timing of each Ca2+ spike is determined by the periodic loading of the endoplasmic reticulum with Ca2+ (Figure 1). The best way of describing how this oscillator might function is to fol ...
... Luminal loading Ca2+ oscillation model The main feature of the luminal loading Ca2+ oscillation model is that the timing of each Ca2+ spike is determined by the periodic loading of the endoplasmic reticulum with Ca2+ (Figure 1). The best way of describing how this oscillator might function is to fol ...
Clostridium and Bacillus Binary Enterotoxins
... and gastrointestinal tracts of insects and animals, as well as humans. Both genera grow in low-oxygen environments; however, the clostridia are better adapted for anaerobic life with varying aerotolerance among different species. Pathogenic Clostridium and Bacillus species have developed unique mech ...
... and gastrointestinal tracts of insects and animals, as well as humans. Both genera grow in low-oxygen environments; however, the clostridia are better adapted for anaerobic life with varying aerotolerance among different species. Pathogenic Clostridium and Bacillus species have developed unique mech ...
golgi apparatus, gerl, and lysosomes of neurons in rat dorsal root
... 0.025% CaC1 2 in 0 .09 M cacodylate buffer, pH 7 .4 (all expressed as final concentrations) . About 12 ganglia were separated from the spinal cord within 1-3 min, and then placed into fresh ice-chilled fixative . After a total fixation time of 60 min, the ganglia were rinsed in three changes of cold ...
... 0.025% CaC1 2 in 0 .09 M cacodylate buffer, pH 7 .4 (all expressed as final concentrations) . About 12 ganglia were separated from the spinal cord within 1-3 min, and then placed into fresh ice-chilled fixative . After a total fixation time of 60 min, the ganglia were rinsed in three changes of cold ...
Composition and production of thiol constituents induced
... ECnG). Phytochelatins with nranging from 2 to 11 have been described [4,5]. Although glycine is the most common terminal amino acid, glutamic acid [6], serine [7], and βalanine [8] have been found instead of glycine in several species. Moreover, the terminal amino acid does not appear in desglycyl- ...
... ECnG). Phytochelatins with nranging from 2 to 11 have been described [4,5]. Although glycine is the most common terminal amino acid, glutamic acid [6], serine [7], and βalanine [8] have been found instead of glycine in several species. Moreover, the terminal amino acid does not appear in desglycyl- ...
Stacks off tracks
... bioRxiv preprint first posted online Mar. 14, 2017; doi: http://dx.doi.org/10.1101/115840. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
... bioRxiv preprint first posted online Mar. 14, 2017; doi: http://dx.doi.org/10.1101/115840. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
A PP2A-B55 recognition signal controls substrate
... Figure 1. B55 substrates share a unique temporal signature. (A) A schematic of the BEG pathway showing regulation of generic Cdk1 substrates at the metaphase to anaphase transition. Idealized temporal dephosphorylation profiles for B55 substrates in control (Control), B55 (-B55), and Greatwall–MAS ...
... Figure 1. B55 substrates share a unique temporal signature. (A) A schematic of the BEG pathway showing regulation of generic Cdk1 substrates at the metaphase to anaphase transition. Idealized temporal dephosphorylation profiles for B55 substrates in control (Control), B55 (-B55), and Greatwall–MAS ...
Auxin Transport Promotes Arabidopsis Lateral Root Initiation
... order of the initial transverse divisions have not been characterized in detail in Arabidopsis. Auxin represents a key regulator of lateral root development (Blakely et al., 1982; Laskowski et al., 1995). Several auxin-related mutants have been described in Arabidopsis that arrest lateral root forma ...
... order of the initial transverse divisions have not been characterized in detail in Arabidopsis. Auxin represents a key regulator of lateral root development (Blakely et al., 1982; Laskowski et al., 1995). Several auxin-related mutants have been described in Arabidopsis that arrest lateral root forma ...
Sanguinarine (Pseudochelerythrine) Is a Potent Inhibitor of NF
... and then exposed them to TNF. Sanguinarine was also added at the same time as 0 min and 5, 15, and 30 min after TNF. In every case, TNF was present for 30 min. As shown in Fig. 2C, coincubation of cells with sanguinarine and TNF was as effective as preincubation in blocking the activation of NF-kB. ...
... and then exposed them to TNF. Sanguinarine was also added at the same time as 0 min and 5, 15, and 30 min after TNF. In every case, TNF was present for 30 min. As shown in Fig. 2C, coincubation of cells with sanguinarine and TNF was as effective as preincubation in blocking the activation of NF-kB. ...
The p57 CDKi integrates stress signals into cellcycle progression to
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
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... between 12 and 24 h of culture and a shift in the extracellular matrix composition from a mesenchymal to an epithelial type. Based on these observations we have suggested that the responsiveness to transferrin is acquired during the induction of kidney mesenchyme (Ekblom et al. 1983a). In the presen ...
... between 12 and 24 h of culture and a shift in the extracellular matrix composition from a mesenchymal to an epithelial type. Based on these observations we have suggested that the responsiveness to transferrin is acquired during the induction of kidney mesenchyme (Ekblom et al. 1983a). In the presen ...
The Role of the Terminal and Subterminal Oxidation
... Summit, NJ, USA). All three strains were very similar. Colonies were pink when grown on propane/AMS agar plates but growth in liquid culture on propane did not result in visible pigmentation. They were obligately aerobic, Gram-positive rods; they were not acid-fast, did not form spores or contain me ...
... Summit, NJ, USA). All three strains were very similar. Colonies were pink when grown on propane/AMS agar plates but growth in liquid culture on propane did not result in visible pigmentation. They were obligately aerobic, Gram-positive rods; they were not acid-fast, did not form spores or contain me ...
Two different but converging messenger pathways to intracellular
... 1997; Lee, 1997). In sea urchin eggs, it would appear that the NAADP-sensitive stores are physically separated from those sensitive to IP3 and cADPR (Genazzani and Galione, 1997; Lee, 1997). In pancreatic acinar cells, NAADP has recently been shown to be involved in CCK-evoked Ca2+ signalling (Cance ...
... 1997; Lee, 1997). In sea urchin eggs, it would appear that the NAADP-sensitive stores are physically separated from those sensitive to IP3 and cADPR (Genazzani and Galione, 1997; Lee, 1997). In pancreatic acinar cells, NAADP has recently been shown to be involved in CCK-evoked Ca2+ signalling (Cance ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.