Nonsense-mediated mRNA decay - Case Western Reserve University
... Nonsense-mediated mRNA decay is a surveillance pathway that reduces errors in gene expression by eliminating aberrant mRNAs that encode incomplete polypeptides. Recent experiments suggest a working model whereby premature and normal translation termination events are distinct as a consequence of the ...
... Nonsense-mediated mRNA decay is a surveillance pathway that reduces errors in gene expression by eliminating aberrant mRNAs that encode incomplete polypeptides. Recent experiments suggest a working model whereby premature and normal translation termination events are distinct as a consequence of the ...
Interactions of liposomes and lipid-based carrier systems with blood
... different anionic phospholipids triggers rapid clearance behavior in CD1 mice [8]. However, the clearance is not solely dependent on the negative surface charge since LUVs expressing phosphatidylserine (PS), phosphatidic acid (PA) or cardiolipin (CL) on their outer surface are all cleared very rapid ...
... different anionic phospholipids triggers rapid clearance behavior in CD1 mice [8]. However, the clearance is not solely dependent on the negative surface charge since LUVs expressing phosphatidylserine (PS), phosphatidic acid (PA) or cardiolipin (CL) on their outer surface are all cleared very rapid ...
Uncoupling insulin signalling by serine/threonine phosphorylation: a
... They contain a conserved PH (pleckstrin homology) domain, located at their N-terminus, that serves to anchor the IRS proteins to membrane phosphoinositides, which helps to localize the IRS proteins in close proximity to the IR [4]. The PH domain is flanked by a PTB (phospho-Tyr binding) domain that ...
... They contain a conserved PH (pleckstrin homology) domain, located at their N-terminus, that serves to anchor the IRS proteins to membrane phosphoinositides, which helps to localize the IRS proteins in close proximity to the IR [4]. The PH domain is flanked by a PTB (phospho-Tyr binding) domain that ...
Characterization of the Enzymatic Component of the ADP
... Certain strains of Clostridium difficile produce the ADP-ribosyltransferase CDT, which is a binary actin ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component ...
... Certain strains of Clostridium difficile produce the ADP-ribosyltransferase CDT, which is a binary actin ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component ...
The HSP90-SGT1 Chaperone Complex for NLR
... tor APAF-1, R protein, and the nematode CED-4). This module is often referred to as the NB-ARC unit (Figure 1). The corresponding unit in vertebrate NLR proteins is called NACHT (domain present in NAIP, CIITA, HET-E, and TP1) (56). The NB-ARC and NACHT domains have ATPase activity and are thought to ...
... tor APAF-1, R protein, and the nematode CED-4). This module is often referred to as the NB-ARC unit (Figure 1). The corresponding unit in vertebrate NLR proteins is called NACHT (domain present in NAIP, CIITA, HET-E, and TP1) (56). The NB-ARC and NACHT domains have ATPase activity and are thought to ...
The Activity of a Developmentally Regulated Cysteine Proteinase Is
... release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and characterized: CWP1 (26 kDa) and CWP2 (39 kDa). Expression ...
... release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and characterized: CWP1 (26 kDa) and CWP2 (39 kDa). Expression ...
Periplasmic folding factors in Gram-negative bacteria
... approximately 600 Da (2). This barrier function protects bacteria from harmful substances in their environment, such as antibiotics and detergents. Defects in the biogenesis of the outer membrane are known to increase the sensitivity of bacteria for such harmful substances (3). The outer membrane is ...
... approximately 600 Da (2). This barrier function protects bacteria from harmful substances in their environment, such as antibiotics and detergents. Defects in the biogenesis of the outer membrane are known to increase the sensitivity of bacteria for such harmful substances (3). The outer membrane is ...
Characterization of a 30S Ribsomal Subunit Intermediate Found in
... their unconditional love and support of the years. They encouraged me to excel above and beyond what my eyes could see, even when things did not seem possible. My most heartfelt and deep gratitude goes to my grandmother, who has always been my number one fan and cheerleader. I will forever be gratef ...
... their unconditional love and support of the years. They encouraged me to excel above and beyond what my eyes could see, even when things did not seem possible. My most heartfelt and deep gratitude goes to my grandmother, who has always been my number one fan and cheerleader. I will forever be gratef ...
Chapter 10
... into the endoplasmic reticulum where protein folding and processing take place. From the endoplasmic reticulum, proteins are transported in vesicles to the Golgi apparatus where they are further processed and sorted for transport to endosomes, lysosomes, the plasma membrane, or secretion from the ce ...
... into the endoplasmic reticulum where protein folding and processing take place. From the endoplasmic reticulum, proteins are transported in vesicles to the Golgi apparatus where they are further processed and sorted for transport to endosomes, lysosomes, the plasma membrane, or secretion from the ce ...
The Evolution of SMC Proteins: Phylogenetic Analysis and Structural
... SMC-containing complexes, as shown by mutagenesis of the ATP-binding domain (Chuang, Albertson, and Meyer 1994; Verkade et al. 1999; Fousteri and Lehmann 2000; Hirano et al. 2001) or the use of nonhydrolysable ATP analogs (Kimura and Hirano 1997). Based on the identification of these motifs at the e ...
... SMC-containing complexes, as shown by mutagenesis of the ATP-binding domain (Chuang, Albertson, and Meyer 1994; Verkade et al. 1999; Fousteri and Lehmann 2000; Hirano et al. 2001) or the use of nonhydrolysable ATP analogs (Kimura and Hirano 1997). Based on the identification of these motifs at the e ...
This Article Abstract Full Text (PDF) Alert me when this article is cited
... WzcCPS include the site(s) of phosphorylation of the protein and that phosphorylation at this site is essential for assembly of HMW K30 CPS (43). This C-terminal region is tyrosine rich, with seven tyrosine residues in the last 17 amino acids. Similar features were reported for a number of Wzc homol ...
... WzcCPS include the site(s) of phosphorylation of the protein and that phosphorylation at this site is essential for assembly of HMW K30 CPS (43). This C-terminal region is tyrosine rich, with seven tyrosine residues in the last 17 amino acids. Similar features were reported for a number of Wzc homol ...
Supplementary Table 1
... Based on our findings with Gcn4, we propose a class of regulatory factors whose activity is required in a reaction, but whose subsequent turnover or removal promotes completion of the reaction or subsequent reaction cycles. Such ‘activation by destruction’ appears to play a role in numerous cellular ...
... Based on our findings with Gcn4, we propose a class of regulatory factors whose activity is required in a reaction, but whose subsequent turnover or removal promotes completion of the reaction or subsequent reaction cycles. Such ‘activation by destruction’ appears to play a role in numerous cellular ...
The proposed channel-enzyme transient receptor potential
... membranes of cells – for example to travel in or out of a cell, or between different parts of the same cell. Almost all ion channels are gated, meaning that they can open and close in response to different signals. For instance, so-called ligand gated channels open in response to binding of some sma ...
... membranes of cells – for example to travel in or out of a cell, or between different parts of the same cell. Almost all ion channels are gated, meaning that they can open and close in response to different signals. For instance, so-called ligand gated channels open in response to binding of some sma ...
Processing Synthesis of Cellulase from Ripening
... Cellulase protein was detected by incubation in TBS with cellulase antiserum (1:15,000 dilution) followed by two washes in TBS supplemented with 0.05% Tween-20. The blot was then incubated in TBS with horseradish peroxidase-conjugated goat anti-rabbit IgG (1:2000 dilution) (Bio-Rad) and, following t ...
... Cellulase protein was detected by incubation in TBS with cellulase antiserum (1:15,000 dilution) followed by two washes in TBS supplemented with 0.05% Tween-20. The blot was then incubated in TBS with horseradish peroxidase-conjugated goat anti-rabbit IgG (1:2000 dilution) (Bio-Rad) and, following t ...
Facing extremes: archaeal surface-layer (glyco)proteins
... chains, with sulfated glucuronic acid moieties attached to asparagine-linked glucose residues predominating and a single chain of a sulfated repeating unit pentasaccharide linked through N-acetylgalactosamine positioned at the 2-asparagine position of the protein (Lechner & Wieland, 1989). It remain ...
... chains, with sulfated glucuronic acid moieties attached to asparagine-linked glucose residues predominating and a single chain of a sulfated repeating unit pentasaccharide linked through N-acetylgalactosamine positioned at the 2-asparagine position of the protein (Lechner & Wieland, 1989). It remain ...
Facing extremes: archaeal surface-layer (glyco)proteins
... chains, with sulfated glucuronic acid moieties attached to asparagine-linked glucose residues predominating and a single chain of a sulfated repeating unit pentasaccharide linked through N-acetylgalactosamine positioned at the 2-asparagine position of the protein (Lechner & Wieland, 1989). It remain ...
... chains, with sulfated glucuronic acid moieties attached to asparagine-linked glucose residues predominating and a single chain of a sulfated repeating unit pentasaccharide linked through N-acetylgalactosamine positioned at the 2-asparagine position of the protein (Lechner & Wieland, 1989). It remain ...
Basic region of residues 228-231 of protein kinase CK1[alpha] is
... in these assays was produced by 35S labeling through an in vitro transcription-translation system or by allowing CK1a to autophosphorylate with 32P. Alternatively, the presence of active CK1a bound to axin on the sepharose beads can be assayed by determining its capacity to phosphorylate a specific ...
... in these assays was produced by 35S labeling through an in vitro transcription-translation system or by allowing CK1a to autophosphorylate with 32P. Alternatively, the presence of active CK1a bound to axin on the sepharose beads can be assayed by determining its capacity to phosphorylate a specific ...
Fern Gametophytes Grown under Different Light Spectra 1
... B-, and W-gametophytes, equal amounts of protein extracts were separated on SDS-PAGE gels. Thereafter the gels were blotted onto nitrocellulose filters and probed with polyclonal antibodies raised in rabbit against either SDS-treated spinach LSU or SSU. Figures 3 and 4 show typical immunoblots using ...
... B-, and W-gametophytes, equal amounts of protein extracts were separated on SDS-PAGE gels. Thereafter the gels were blotted onto nitrocellulose filters and probed with polyclonal antibodies raised in rabbit against either SDS-treated spinach LSU or SSU. Figures 3 and 4 show typical immunoblots using ...
Defining the impact on yeast ATP synthase of two pathogenic
... acids that is removed during assembly of the protein [38-41]. Indeed, if this were the case, both mutant proteins would have the same migration rate, which is not observed. Atp6p like other very hydrophobic proteins has aberrant electrophoretic properties; while it migrates as an 21 KDa protein it h ...
... acids that is removed during assembly of the protein [38-41]. Indeed, if this were the case, both mutant proteins would have the same migration rate, which is not observed. Atp6p like other very hydrophobic proteins has aberrant electrophoretic properties; while it migrates as an 21 KDa protein it h ...
Preparation of Lentiviral expression construct DNA
... quantification of p53 in a 96-well format . Double-stranded oligonucleotides containing a p53 consensus binding site are immobilized in a 96-well plate. The p53 proteins present in nuclear extracts are captured by the immobilized oligonucleotides specifically and then detected by a p53 antibody and ...
... quantification of p53 in a 96-well format . Double-stranded oligonucleotides containing a p53 consensus binding site are immobilized in a 96-well plate. The p53 proteins present in nuclear extracts are captured by the immobilized oligonucleotides specifically and then detected by a p53 antibody and ...
The SMN Complex Is Associated with snRNPs throughout Their
... The common neurodegenerative disease spinal muscular atrophy is caused by reduced levels of the survival of motor neurons (SMN) protein. SMN associates with several proteins (Gemin2 to Gemin6) to form a large complex which is found both in the cytoplasm and in the nucleus. The SMN complex functions ...
... The common neurodegenerative disease spinal muscular atrophy is caused by reduced levels of the survival of motor neurons (SMN) protein. SMN associates with several proteins (Gemin2 to Gemin6) to form a large complex which is found both in the cytoplasm and in the nucleus. The SMN complex functions ...
Article - Andrej Sali
... et al., 2006; Selmer et al., 2006; Schuwirth et al., 2005). In addition, structures of programmed bacterial ribosomes have defined a path for the translocation of mRNA and tRNAs through the intersubunit space (Yusupova et al., 2001, 2006; Korostelev et al., 2006; Selmer et al., 2006; Berk et al., 20 ...
... et al., 2006; Selmer et al., 2006; Schuwirth et al., 2005). In addition, structures of programmed bacterial ribosomes have defined a path for the translocation of mRNA and tRNAs through the intersubunit space (Yusupova et al., 2001, 2006; Korostelev et al., 2006; Selmer et al., 2006; Berk et al., 20 ...
PapD-like chaperones and pilus biogenesis
... complexed forms of the chaperone are virtually superimposable, with the exception of the F1 ᎐G1 loop, as discussed below. The FimH adhesin consists of two domains: a pilin domain and a receptor-binding domain. The PapK pilin and the pilin domain of FimH have Ig folds; however, they lack the seventh ...
... complexed forms of the chaperone are virtually superimposable, with the exception of the F1 ᎐G1 loop, as discussed below. The FimH adhesin consists of two domains: a pilin domain and a receptor-binding domain. The PapK pilin and the pilin domain of FimH have Ig folds; however, they lack the seventh ...
Promega Notes 39: Stabilized TMB Substrate for Horseradish
... complex mixtures. Two popular immunodetection methods are "dot" blots, in which antigen-containing solutions are spotted directly onto a membrane, and Western blots (1,2), in which proteins are transferred to a membrane following SDS-polyacrylamide gel electrophoresis (3). A common method for the de ...
... complex mixtures. Two popular immunodetection methods are "dot" blots, in which antigen-containing solutions are spotted directly onto a membrane, and Western blots (1,2), in which proteins are transferred to a membrane following SDS-polyacrylamide gel electrophoresis (3). A common method for the de ...
The ribosome — a macromolecular machine par excellence
... As in the case of EF-G, the action of EF-Tu requires energy released by GTP hydrolysis. Much of this information has been known for years (see [5]), but the way the various components of the protein synthetic machinery interact in three dimensions remained a mystery until the advent of cryo-electron ...
... As in the case of EF-G, the action of EF-Tu requires energy released by GTP hydrolysis. Much of this information has been known for years (see [5]), but the way the various components of the protein synthetic machinery interact in three dimensions remained a mystery until the advent of cryo-electron ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.