The presence of monoglucosylated N196
... Glycosylation is an important cellular modification, and it is related to many human diseases and developmental defects [1]. It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays critical roles during a variety of cellular processes, such a ...
... Glycosylation is an important cellular modification, and it is related to many human diseases and developmental defects [1]. It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays critical roles during a variety of cellular processes, such a ...
A New Subunit of Cytochrome b6f Complex Undergoes Reversible
... upon state transitions: changes in the redox state of plastoquinones (PQs) that bind to cytb6f complexes allow a photosynthetic cell to adapt to changes in light quality as well as to changes in intracellular ATP levels (4). At the molecular level, plastoquinol binding at the Qo site of cytb6f compl ...
... upon state transitions: changes in the redox state of plastoquinones (PQs) that bind to cytb6f complexes allow a photosynthetic cell to adapt to changes in light quality as well as to changes in intracellular ATP levels (4). At the molecular level, plastoquinol binding at the Qo site of cytb6f compl ...
A Survey of Flexible Protein Binding Mechanisms and their
... quantitative prediction of the dynamics and specificity of protein recognition and assembly. Such understanding may lead to the ability to design partners that form more stable complexes, which can then act as “network” drugs. Understanding interactions will also help us to find ways of inhibiting p ...
... quantitative prediction of the dynamics and specificity of protein recognition and assembly. Such understanding may lead to the ability to design partners that form more stable complexes, which can then act as “network” drugs. Understanding interactions will also help us to find ways of inhibiting p ...
Structure and mechanism of ATP-dependent phospholipid transporters
... Fig. 1. General structure of ABC transporters and P-type ATPases. (A) Secondary structure of ABC transporters. The ABC transporter exists both as a monomer full transporter and as a dimer consisting of two half transporters. In either case, this type of transporter contains two transmembrane domains ...
... Fig. 1. General structure of ABC transporters and P-type ATPases. (A) Secondary structure of ABC transporters. The ABC transporter exists both as a monomer full transporter and as a dimer consisting of two half transporters. In either case, this type of transporter contains two transmembrane domains ...
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... introduced in 1942 by Waksman and his collaborators. At first, antibiotics included only microbially-originated chemical substances, which inhibit the growth or the metabolic activities of bacteria and other micro-organisms. Recently, the concept of antibiotics has been enlarged to include anti-fung ...
... introduced in 1942 by Waksman and his collaborators. At first, antibiotics included only microbially-originated chemical substances, which inhibit the growth or the metabolic activities of bacteria and other micro-organisms. Recently, the concept of antibiotics has been enlarged to include anti-fung ...
A Personal Account of Chaperonin History
... of GroEL. It was a 14-mer consisting of two heptameric rings stacked back to back. It also hydrolyzed ATP. Quite what GroEL did in the cell and how it was involved in phage morphogenesis was not known at this stage. Nevertheless, Hohn et al. (10) wrote “In groE mutants, bacteriophage T4 capsid prote ...
... of GroEL. It was a 14-mer consisting of two heptameric rings stacked back to back. It also hydrolyzed ATP. Quite what GroEL did in the cell and how it was involved in phage morphogenesis was not known at this stage. Nevertheless, Hohn et al. (10) wrote “In groE mutants, bacteriophage T4 capsid prote ...
the function and synthesis of ribosomes
... >95% conserved across all three kingdoms of life, supporting its key functional role. The domain is stabilized by the long extensions of four r-proteins, but these are too far away to participate directly in catalysis. From this, it was concluded that the peptidyl-transfer reaction is catalysed by R ...
... >95% conserved across all three kingdoms of life, supporting its key functional role. The domain is stabilized by the long extensions of four r-proteins, but these are too far away to participate directly in catalysis. From this, it was concluded that the peptidyl-transfer reaction is catalysed by R ...
Systematic Characterisation of Cellular Localisation and
... focus of the available methods is, however, to identify the optimal peptides from provided protein sequences, while little effort has been made towards developing schemes for prioritisation of the optimal protein candidates from a pool of proteins. To this end, increased knowledge about the general ...
... focus of the available methods is, however, to identify the optimal peptides from provided protein sequences, while little effort has been made towards developing schemes for prioritisation of the optimal protein candidates from a pool of proteins. To this end, increased knowledge about the general ...
Clathrinmediated transport: assembly required
... Formation and function of the clathrin scaffold In the mid-1970s, the isolation of CCVs by density centrifugation allowed B. Pearse (Cambridge, UK) to purify clathrin, one of the main components of these structures (Pearse, 1976). The assembly unit of clathrin, commonly referred to as the ‘triskelio ...
... Formation and function of the clathrin scaffold In the mid-1970s, the isolation of CCVs by density centrifugation allowed B. Pearse (Cambridge, UK) to purify clathrin, one of the main components of these structures (Pearse, 1976). The assembly unit of clathrin, commonly referred to as the ‘triskelio ...
In vivo assays to study histone ubiquitylation
... methylation of adjacent lysine residues in the H3 N tail by several SET domain proteins [40–43]. These results are not consistent with a model in which histone ubiquitylation merely serves a structural role to open chromatin. Ubiquitylation of proteins destined for degradation occurs through a conce ...
... methylation of adjacent lysine residues in the H3 N tail by several SET domain proteins [40–43]. These results are not consistent with a model in which histone ubiquitylation merely serves a structural role to open chromatin. Ubiquitylation of proteins destined for degradation occurs through a conce ...
Functional analysis of organophosphorus hydrolase variants with high degradation
... The importance of the additional K185R and A80V mutations in 22A11 was further investigated by comparing the degradation rates of other substrates containing the larger diethyl side chain such as paraoxon, parathion and coumaphos (Figure 1) with whole cells expressing the different variants on the c ...
... The importance of the additional K185R and A80V mutations in 22A11 was further investigated by comparing the degradation rates of other substrates containing the larger diethyl side chain such as paraoxon, parathion and coumaphos (Figure 1) with whole cells expressing the different variants on the c ...
Ribosomal proteins L5 and L15 Ivailo Simoff in vivo
... Protein synthesis is a complex, highly regulated and energy consuming process, during which a large ribonucleoprotein particle called the ribosome, synthesizes new proteins, according to the specification laid down in the genes. The eukaryotic ribosome consists of two unequal parts called: small and ...
... Protein synthesis is a complex, highly regulated and energy consuming process, during which a large ribonucleoprotein particle called the ribosome, synthesizes new proteins, according to the specification laid down in the genes. The eukaryotic ribosome consists of two unequal parts called: small and ...
Protein Dynamics in the Plant Extracellular Space
... on the metabolic processes occurring in the ECS under abiotic and biotic stresses, as well as under non-challenged conditions. The large majority of the proteins detected are involved in “cell wall organization and biogenesis”, “response to stimulus” and “protein metabolism”. It becomes apparent tha ...
... on the metabolic processes occurring in the ECS under abiotic and biotic stresses, as well as under non-challenged conditions. The large majority of the proteins detected are involved in “cell wall organization and biogenesis”, “response to stimulus” and “protein metabolism”. It becomes apparent tha ...
Title Scaffold proteins in mammalian MAP kinase
... the axon in cultured neurons. Together, these studies indicate that some scaffold proteins such as JIP1 and JSAP1 may actually translocate MAPK modules along microtubules with kinesin to a specific destination. To date, however, it is not known whether the MAPK modules themselves are also involved i ...
... the axon in cultured neurons. Together, these studies indicate that some scaffold proteins such as JIP1 and JSAP1 may actually translocate MAPK modules along microtubules with kinesin to a specific destination. To date, however, it is not known whether the MAPK modules themselves are also involved i ...
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... rotein kinases are key components of signal transduction pathways that regulate many aspects of eukaryotic biology (1, 2). The protein kinase gene family is one of the largest in eukaryotic organisms and typically constitutes almost 2% of all protein-encoding genes (3, 4). In general, these enzymes ...
... rotein kinases are key components of signal transduction pathways that regulate many aspects of eukaryotic biology (1, 2). The protein kinase gene family is one of the largest in eukaryotic organisms and typically constitutes almost 2% of all protein-encoding genes (3, 4). In general, these enzymes ...
Antioxidant and antimicrobial activity of lecithin free egg yolk protein
... [3]. Two peptides obtained from lecithin-free egg yolk hydrolysate also exhibited antioxidant activity in a linoleic acid model system. These peptides, composed of 10 and 15 amino acid residues, both contain a leucine residue at their N- therminal positions [10]. Phosvitin, which has been recognized ...
... [3]. Two peptides obtained from lecithin-free egg yolk hydrolysate also exhibited antioxidant activity in a linoleic acid model system. These peptides, composed of 10 and 15 amino acid residues, both contain a leucine residue at their N- therminal positions [10]. Phosvitin, which has been recognized ...
The Proteomics Big Challenge for Biomarkers and New Drug
... Functional proteomics has been widely applied to mapping signaling pathways in a number of pathologies and the vast literature published in the last ten years exceeds the scope of a single review. Therefore, only a few examples will be illustrated to describe some of the new paradigms in signaling n ...
... Functional proteomics has been widely applied to mapping signaling pathways in a number of pathologies and the vast literature published in the last ten years exceeds the scope of a single review. Therefore, only a few examples will be illustrated to describe some of the new paradigms in signaling n ...
Twins take the job
... elevated levels of hRRP6, arguing that these enzymes have partially redundant functions. ...
... elevated levels of hRRP6, arguing that these enzymes have partially redundant functions. ...
ABSTRACT Title of Document:
... The first anti-apoptotic gene that was identified by our lab, nuoG, is a part of the nuo operon and codes for a protein NDH-1. NDH-1 is a proton-pumping NADH ubiquinone reductase, and is also called respiratory complex 1. It is the first enzyme of the bacterial and mitochondrial respiratory chain. I ...
... The first anti-apoptotic gene that was identified by our lab, nuoG, is a part of the nuo operon and codes for a protein NDH-1. NDH-1 is a proton-pumping NADH ubiquinone reductase, and is also called respiratory complex 1. It is the first enzyme of the bacterial and mitochondrial respiratory chain. I ...
The EMBO Journal (2002) 21, 1704–1712
... in transcriptional repression (Turner, 2000). They are believed to be targeted to specific promoters through physical interactions with promoter-specific transcription factors. Through their effect on the transcription of specific genes, HATs and HDACs are involved in the control of many cellular pr ...
... in transcriptional repression (Turner, 2000). They are believed to be targeted to specific promoters through physical interactions with promoter-specific transcription factors. Through their effect on the transcription of specific genes, HATs and HDACs are involved in the control of many cellular pr ...
“The function and synthesis of ribosomes.” Nature Reviews Mol Cell
... >95% conserved across all three kingdoms of life, supporting its key functional role. The domain is stabilized by the long extensions of four r-proteins, but these are too far away to participate directly in catalysis. From this, it was concluded that the peptidyl-transfer reaction is catalysed by R ...
... >95% conserved across all three kingdoms of life, supporting its key functional role. The domain is stabilized by the long extensions of four r-proteins, but these are too far away to participate directly in catalysis. From this, it was concluded that the peptidyl-transfer reaction is catalysed by R ...
Molecular paleontology and complexity in the last eukaryotic
... Figure 2. Possible scenarios for the FECA to LECA transition. The top schema depicts the periods of prokaryotic (blue) and eukaryotic (red) evolution, separated by a transition period, which is expanded for clarity. Relative distances on the x-axis are arbitrary, and note that the earliest times sho ...
... Figure 2. Possible scenarios for the FECA to LECA transition. The top schema depicts the periods of prokaryotic (blue) and eukaryotic (red) evolution, separated by a transition period, which is expanded for clarity. Relative distances on the x-axis are arbitrary, and note that the earliest times sho ...
Cytostatic factor: an activity that puts the cell cycle on hold
... the precise role of Rca1 in this process and further studies will be required to determine its relationship with Emi1 and Erp1. The founding member of the family is Emi1, which was first identified in an unbiased screen for human F-box-containing proteins and thus named FBXO5 (Cenciarelli et al., 19 ...
... the precise role of Rca1 in this process and further studies will be required to determine its relationship with Emi1 and Erp1. The founding member of the family is Emi1, which was first identified in an unbiased screen for human F-box-containing proteins and thus named FBXO5 (Cenciarelli et al., 19 ...
The twin arginine protein transport pathway exports multiple
... therefore gives confidence that the subset of proteins predicted to be Tat substrates by both programmes are highly likely to represent real substrates. For S. scabies, 82 proteins are predicted to be Tat substrates by each of the two programmes, and these are listed in Table S1. It was noted from p ...
... therefore gives confidence that the subset of proteins predicted to be Tat substrates by both programmes are highly likely to represent real substrates. For S. scabies, 82 proteins are predicted to be Tat substrates by each of the two programmes, and these are listed in Table S1. It was noted from p ...
Role for Adenosine Triphosphate in Regulating the Assembly and
... washed with methionine-free medium, and the cells were suspended by gentle scraping with a rubber policeman into 1 ml of labeling medium at the appropriate temperature. For experiments which required depletion of cellular ATP, the labeling medium lacked glucose (Batch et al., 1986). Resuspended cell ...
... washed with methionine-free medium, and the cells were suspended by gentle scraping with a rubber policeman into 1 ml of labeling medium at the appropriate temperature. For experiments which required depletion of cellular ATP, the labeling medium lacked glucose (Batch et al., 1986). Resuspended cell ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.