Download RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
KARNATAKA, BANGALORE.
SYNOPSIS
BY
Dr RAJEEVE B
TITLE OF TOPIC
“HEPATOPROTECTIVE ACTIVITY OF
MADAYANTIKA ( LAWSONIA INERMIS Linn.) –
AN EXPERIMENTAL EVALUATION”
2010 - 2011
Shri. J.G.C.H.S Ayurvedic Medical College
Ghataprabha, Belgaum.
FROM,
Dr. RAJEEVE B
I M.D, DEPT. OF DRAVYAGUNA VIJNANA
POST GRADUATE STUDIES,
SHRI. J.G.C.H.S. AYURVEDIC MEDICAL COLLEGE
GHATAPRABHA, BELGAUM
TO,
THE REGISTRAR
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
JAYANAGAR, 4TH BLOCK, BANGALORE
BANGALORE, KARNATAKA
THROUGH
THE PRINCIPAL & HOD DRAVYAGUNA VIJNANA
POST GRADUATE STUDIES
SHRI. J.G.C.H.S. AYURVEDIC MEDICAL COLLEGE
GHATAPRABHA, BELGAUM
Sub: Submission of completed proforma for Registration of synopsis for dissertation
Respected Sir,
I request you to kindly register the below mentioned subject against my name for the
submission of dissertation to the Rajiv Gandhi University of Health Sciences, Karnataka,
Bangalore for the partial fulfilment of post graduation course in the specialization of Dravya
Guna vijnana M.D.(Ayu)
TITLE OF DISSERTATION:
“HEPATOPROTECTIVE ACTIVITY OF MADAYANTIKA ( LAWSONIA INERMIS
Linn.) – AN EXPERIMENTAL EVALUATION”
I am enclosing completed proforma for registration of subject for dissertation.
Thanking you
Place : Ghataprabha
Your’s faithfully
Date: 13/04/2011
Dr. RAJEEVE B
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
KARNATAKA, BANGALORE
ANNEXURE II
COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT FOR
DISSERTATION
1.
NAME OF THE CANDIDATE
:
Dr. RAJEEVE B
AND ADDRESS
I yr M.D. (Ayu)
S/O E S BHASKARAN PILLAI,
KRISHNA NIVAS,
MUDICKAL (PO),
ERNAKULAM - Dist,
State- KERALA, Pin-683547
Email: [email protected]
2.
NAME OF THE INSTITUTE
:
Shri. J.G.C.H Society’s
Ayurvedic Medical College,
Ghataprabha , Belgaum
3.
COURSE OF STUDY
:
Ayurveda Vaachaspathi M.D (Ay)
DRAVYA GUNA VIJNAN
4.
DATE OF ADMISSION OF COURSE
5.
TITLE OF THE TOPIC:
:
12-10-2010
“HEPATOPROTECTIVE ACTIVITY OF
MADAYANTIKA (LAWSONIA INERMIS Linn.) –AN EXPERIMENTAL EVALUATION”
6. BRIEF RESUME OF THE INTENDED WORK:
6.1 Need for studyAyurvedic and Modern Science of Medicine have recognized the importance of Yakrt
(liver). Ayurveda consider it as one among the Kosthangas. It is stated to be the seat of ranjaka
pitta and root of raktavahasrotas. It takes part in various metabolic activities and Bhutagnipaka.
The liver is the largest gland in human body, weighing approximately 3pounds and occupying a
large region mostly on the right side of the body, below diaphragm and behind ribs five to ten.
Liver disease is categorized both by the cause and the effect it has on the liver. Causes
may include infection, injury, exposure to drugs or toxic compounds, and autoimmune process.
Liver disease may affect the capacity of liver to metabolise drugs. An impairment of this organ
and infection caused to it leads to the liver diseases like Alcoholic liver diseases, Hepatitis,
Primary biliary cirrhosis, liver abscess, hemochromatosis and liver cancer1. It is estimated that
liver diseases are among the top ten killer diseases in India, causing lakhs of death every year.
To treat liver diseases of unknown aetiology, the usage of herbal medications with activities like
hepatoprotective, anti-hepatic viruses and stimulation of hepatocyte regeneration has to be
developed. There are more than 87 medicinal plants used in different combinations in the
preparation of patented herbal formulations. It is also used as a single drug therapy by most of
folklore practitioners for hepatic disorders in India e.g. Bhumyamalaki, Kalamegha,
Bhrungaraja, Guduchi etc.
It is reported that the folklore traditional practitioners are using the plant Lawsonia inermis
(Madayantika ) for the treatment of Jaundice. In view of this while exploring the folk remedies it
is found that the traditional tribal practitioners of Waynadu area of Kerala are using the leaves
and roots of the drug Lawsonia inermis (Madayantika) against jaundice. It is a shrub which goes
up to a height of 4meters baring small white flowers. According to Prof P V Sharma2 properties
of Madayantika
are:- Rasa- Tikta,Kashaya: Guna- Leghu,Ruksha, ;Virya- Sita; Vipaka- Katu;
Karma- Kapha Pithahara .and indicated in Kamala, Mutrakricchra, Apasmara,Kandu & Kustha
The objective of this study is to determine the effect and efficacy of Lawsonia inermis
(Madayantika) in the liver and to reveal the hepatoprotective activity of the drug.
6.2 Review of Literature:
1. Literary source:
a) The data of drug Lawsonia inermis (Madayantika ) will be available in various Ayurvedic
books. The modern writers of Ayurveda especially , P.V. Sharma2, Khare C P3 , J L N Sastry4
Nadkarni5 , P K Varrier6 , etc. have also identified and described this plant in detail.
b ) Literature data of hepatoprotectivity and studies will be collected from all the available
modern medicine literature from library of Shri. J.G.C.H Society’s Ayurvedic Medical College,
Ghataprabha , Belgaum
2. Drug Source: The drug Lawsonia inermis Linn (Madayantika ) will be available in herb
garden of Shri. J.G.C.H Society’s Ayurvedic Medical College, Ghataprabha , Belgaum
Previous work done:
1) Effect of an indigenous drug KUTAKI on Jaundice by Pandey A N in 1966, BHU, Varanasi.
2) Experimental evaluation of hepatoprotective activity of Patola (Trichosanthus dioica Roxb) &
Kethaki (Pandanus odovatissRoxb) by Dr R Ilanchezhiyan in 2004 RGUHS, Bangalore
3) Pharmacognostical & Phytochemical study of Parijata w s r to hepato protective activity by Dr
Sarawad sashidhar S in 2005 RGUHS, Bangalore
4).An experimental study on the hepatoprotective action of Udumbara (Ficus recemosa Linn) by
Pushpa S in 1994, Kerala University, Trivandrum
5). Experimental Evaluation of Hepato protective Effect of Kakamaci (Solanum nigrum L)
by Dr Sanjeev L Athani in 2003, RGUHS, Bangalore
6.3 Objective of the study1. To experiment and observe the hepatoprotective effect of the leaves of Lawsonia inermis
Linn.
2. To experiment and observe the hepatoprotective effect of the roots of Lawsonia inermis
Linn.
3. To compare the hepatoprotective activity of leaves and roots of Lawsonia inermis Linn
4. To develop a cost effective hepatoprotective single herb therapy for liver diseases.
7. Materials and Methods-
7.1 Source of data- Single blind study will be conducted at govt approved animal house on
albino rats.
7.2 Method of collection of dataThe experimental study will be carried on four groups. As per the inclusion criteria 24 albino rats
of either sex will be selected for the study
a) Inclusion CriteriaThe Sprague dawley type of Albino rats7 of either sex (weighing between 150 and 200gram)
bred in animal house will be selected for the study.
b)Assessment Criteria- The estimation of enzymes like serum glutamic oxaloacetic
transaminase (S.G.O.T.), serum glutamic pyruvic transaminase (S.G.P.T.), bilirubin and proteins
are considered as the criteria for the assessment of the result, along with the histopathological
evaluations.
c) InvestigationsLiver Function Test:
e) Study design (Interventions)Study will be conducted at govt approved animal houses on Albino rats. The
experimental study will be carried on four groups.
Group -I will be the control group which will be treated with the distilled water.
Group II - will be treated with hepato toxic reagent Carbon tetra chloride (CCl4)
Group III – will be treated with root kwatha of drug Lawsonia inermis Linn
Group IV- will be treated with leaf swarasa of drug Lawsonia inermis Linn
In various liver disorders the serum enzyme levels like serum glutamic oxaloacetic transaminase
(S.G.O.T.), serum glutamic pyruvic transaminase (S.G.P.T.) and the serum levels of bilirubin
etc., are elevated and the serum protein levels are decreased. To produce such a condition,
experimentally, carbon tetrachloride will be given in Albino rats as per the approved methods.
The estimation of above said enzymes, bilirubin and proteins will be considered as the criteria
for the assessment of the result, along with the histopathological evaluations.
7.3 Does the study require any investigations or interventions to be done on patients or
other humans or animals? If so please describe briefly.
Yes .The study will be conducted at govt approved animal houses on albino rats .The
Sprague dawley7 type of Albino rats of either sex weighing between 150-200gram in animal
house will be selected for the study. 24 animals will be separated in 4 groups each with six
animals for the study.
7.4 Has ethical clearance been obtained from in case of 7.3?
Yes, clearance has been obtained through the ethical committee formulated
in the institution.
8. References1. Anthony S. Fauci Dr. et.al ‘Harrison’s’ Principles of internal medicine, Vol-II, Mc Graw
Hill Medical New York- Reprint, Edition- 20th, Part-2,Chapter-23, pp.935 - 996, 2006
2. Sharma. P. V Prof, Dravyaguna Vijnana, Vol.II (Vegetable Drugs), reprint,
Chaukhamba Bharati Academy, Varanasi, pp180 – 182, 2001
3. Khare C P ,Indian Medicinal Plants An Illustrated Dictionary Second Indian Reprint,
Springer India Pvt Ltd,New Delhi ,pp 366, 2008
4. Sastry Dr J L N Illustrated DravyagunaVijnana Vol.II ,First Edition, Chaukhambha
Orientalia, Varanasi, pp 766-767, 2004
5. Nadkarni Dr. K M , Indian Materia Medica , Vol.I , Reprint , Popular Prakashan Pvt.
Ltd. Mumbai , pp 730-732, 2002
6. Varrier P K Dr. et.al Indian Medicinal Plants Vol- 3,Reprint ,Arya Vaidya Sala
Kottakkal-Orient Longman Chennai ,pp303-305 ,2004
7 "Online Medical Dictionary". 1998-12-12. http://cancerweb.ncl.ac.uk/cgi
rats,+sprague-dawley. Retrieved 2007-12-15
9 Candidate’s Signature:
10. Remarks of the Guide:
11. Names and Designation of
(In block letters)
11.1
Guide:
:
11.2
Signature
:
11.3
Co-Guide
:
11.4
Signature:
11.5
Head of the
11.6
Signature
12
12.1
12.2
Department
Remarks of Chairman
And Principal.
Signature
:
DR. K.S. KUCHANUR
M.D. (Ayu) Dravya guna
Proff. Dravya guna Department.
Shri J.G.C.H.S.Ayurvedic Medical College.
Ghataprabha – 591321
Tal-Gokak, Dist-Belgaum.Karnataka State
DR. S. V. BAGADE
M.D. (Ayu) Dravya guna
Asst. Proff., Dravya guna Department.
Shri J.G.C.H.S.Ayurvedic Medical College.
Ghataprabha – 591321
Tal-Gokak, Dist-Belgaum.Karnataka State
DR. MAYURESH AGTE
M.D. (Ayu) Dravya guna
Proff. Dravya guna Department.
Shri J.G.C.H.S.Ayurvedic Medical College,
Ghataprabha – 591321
Tal-Gokak, Dist-Belgaum.Karnataka State
:
:
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