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BIOLOGY REVISION Levels of Organisation: LEVEL 1 – Cells Are the basic unit of structure and function in living things. May serve a specific function within the organism Examples- blood cells, nerve cells, bone cells, etc. LEVEL 2 - Tissues Made up of cells that are similar in structure and function and which work together to perform a specific activity Examples - blood, nervous, bone, etc. Humans have 4 basic tissues: connective, epithelial, muscle, and nerve. LEVEL 3 - Organs Made up of tissues that work together to perform a specific activity Examples - heart, brain, skin, etc. LEVEL4 - Organ Systems Groups of two or more tissues that work together to perform a specific function for the organism. Examples - circulatory system, nervous system, skeletal system, etc. The Human body has 11 organ systems - circulatory, digestive, endocrine, excretory (urinary), immune (lymphatic), integumentary, muscular, nervous, reproductive, respiratory, and skeletal. LEVEL 5 - Organisms Entire living things that can carry out all basic life processes. Meaning they can take in materials, release energy from food, release wastes, grow, respond to the environment, and reproduce. Usually made up of organ systems, but an organism may be made up of only one cell such as bacteria or protist. Examples - bacteria, amoeba, mushroom, sunflower, human Cell Structure Animals and Plants Part Function Nucleus Contains genetic material, which controls the activities of the cell Cytoplasm Most chemical processes take place here, controlled by enzymes Cell membrane Controls the movement of substances into and out of the cell Mitochondria Most energy is released by respiration here Ribosomes Protein synthesis happens here Just Plants Part Function Cell wall Strengthens the cell Chloroplasts Contain chlorophyll, which absorbs light energy for photosynthesis Permanent vacuole Filled with cell sap to help keep the cell turgid Diffusion Diffusion is the net movement of particles down a concentration gradient from an area of high concentration to one of low concentration. E.g. Location Particles move From To Gut Digested food products Gut cavity Blood in capillary of villus Lungs Oxygen Alveolar air space Blood circulating around the lungs Osmosis Osmosis is the movement of water from a less concentrated solution to a more concentrated solution through a partially permeable membrane. E.g. Osmosis is important to plants. They gain water by osmosis through their roots. Water moves into plant cells by osmosis, making them turgid or stiff so they that able to hold the plant upright. RUPTURED INCIPIENT PLASMOLOSIS CRENATED TURGID INCIPIENT PLASMOLOSIS FLACCID Active Transport Active transport is the process by which dissolved molecules move across a cell membrane from a lower to a higher concentration. In active transport, particles move against the concentration gradient - and therefore require an input of energy from the cell. Sometimes dissolved molecules are at a higher concentration inside the cell than outside, but, because the organism needs these molecules, they still have to be absorbed. Carrier proteins pick up specific molecules and take them through the cell membrane against the concentration gradient. In humans: during the digestion of food in the small intestine. Carbohydrates are broken down into simple sugars such as glucose. The glucose is absorbed by active transport into the villi, to be passed into the bloodstream and taken around the body. In plants: Cannot absorb minerals by osmosis or diffusion so the root hair cells have carrier molecules on their surface that pick up the minerals and move them into the cell against the concentration gradient. Factors Affecting Movement of Substances In and Out of Cells: Surface Area to Volume Ratio Temperature: Gives particles more energy so increases the speed of movement. Concentration Gradient: The steeper it is the faster movement carried out is. Environment and Feeding Relationships Habitat The place where an organism lives Population All of the members of one species living in an area Community All the living things, animals and plants, living in a particular habitat Ecosystem Habitat + Community Use of Quadrats: Quadrats should be placed randomly so that a representative sample is taken You should look at the results from several quadrats in an area to reduce the effect of an unusual distribution The results are more reliable when you look at the results from many quadrats Quadrats may also be used for slow moving animals such as snails/slugs Trophic Levels Food Chains: A food chain shows the different organisms that live in a habitat, and what eats what. Food Webs: When all the food chains in a habitat are joined up together they form a food web. Pyramids of number, biomass and energy transfer Transfer or substances and energy along a food chain: In an ecosystem there is energy, and this is what allows the organisms to live. This energy mainly comes from one original source: photosynthesis. The plants use this solar energy to produce carbohydrates, which are then consumed by other organisms: transferring the energy. The plants, however, use not all of the energy from sunlight they are far from efficient. Much sunlight misses the plant, is in the wrong wavelength or lost in the inefficiencies of photosynthesis. We use the term gross primary production to refer to the total energy in the molecules of the plant; and net primary production is the surplus energy not used by the plant itself. At each level of the food chain energy is lost because the organism itself for respiration uses it. This limits the number of steps there can be on a food chain as only 10% of energy is transferred between trophic levels. Also if a toxin such as a pesticide gets passed along the food chain, it continually increases in concentration. When it reaches a top consumer at a tertiary level, amounts can often become harmful and affect fertility and therefore population. Human Influences on the Environment: Pollution AIR CFC’s: Chlorofluorocarbons 1930 first produced by Thomas Midgely Junior Used in air conditioning and spray deodorants O-zone pollutant at ground level and blocks UV rays Carbon Monoxide: CO Colourless, odourless + tasteless but highly toxic Produced In petrol engines Can cause acid rain Used in WWII to gas people Attaches to haemoglobin so it can’t carry oxygen – Asphyxiation Carbon Dioxide CO2 Released from burning of (fossil) fuels Keeps in light rays so warming the earth, ice caps melt, low lying countries liable to flooding GREENHOUSE GASES CO2 CH4 H2O CFC’s NO Smoke Smoke Pollution can be domestic and industrial 1956 clean air act Affects respiratory system Sulphur Dioxide SO2 Dissolves in water to form acidic solution Affects pH balance in rivers and lakes harming ecosystem Acid rain can affect photosynthesis Can cause asthma Lead Compounds Pb Lead in atmosphere causes problems in the nervous system in infants and adolescents slowing down mental development. WATER Lead Compounds Domestic plumbing system made of lead leading to lead poisoning Lead weights used by fishermen Pesticides Used to make crops grow Rain washes substances and chemicals away from the soil into streams and lakes, small animals consume it and bioaccumulation occurs E.g. D.D.T Organic has a balance of minerals and is low release Inorganic such as NPK drains into rivers and ponds causing plants to grow very quickly. Then decomposing bacteria multiply rapidly to consume excess dead plant and use up all the oxygen in the water. Sewage has a similar affect. This is EUTROPHICATION. Oil Oil spills from tankers, ships dumping and draining Birds get poisoned, blinded and reduced insulation causing hypothermia. They can’t fly properly due to oil on their wings Blocks light coming through water so stops photosynthesis Cleaning Techniques: Bioremediation Controlled burning Dispersants Secondary containment Skimming Acidification Naturally acidic soils leach into water ways SO2 CO2 NO2 combine with water to make acids Animals like leeches with soft skin absorb acidic water and die; Crustaceans cannot make exoskeletons; buildings made of limestone are eroded. Solutions: o Use cleaner coal o Catalytic convertors o Alkalis in air/water Thermal Pollution It lowers the oxygen’s solubility in water Fish and water mammals are unable to extract oxygen LAND Deforestation Cutting down trees to create more land space, furniture, fuels 12 million hectares cut down per year All forest could be gone by 2050 if rate continues Interferes with Carbon Cycle – CO2 released and not absorbed by trees and surrounding plants Water Cycle messed up – no transpiration, desertification and dryer climate Loss of bio-diverse habitat for many species Soil becomes thin and infertile and is washed away easily (no longer held in place by roots Landfills A huge crater in the ground filed with rubbish then covered over Bacteria break down rubbish releasing methane into surrounding area Many materials are not biodegradable and becomes an eye-sore Seepage of toxic ions into rivers when it rains CYCLES WATER CYCLE: CARBON CYCLE Nitrogen Cycle N2 is inert but humans and plants need it for proteins (and amino acids.) 3 Forms: Nitrates – NO3Ammonium – NH4+ Nitrites – NO2Bacteria: N2 Fixing: N2 to NO3Nitrifying: NH4+ to NO3Decomposers: Waste to NH4+ Denitrifying: NO3- to N2 Lightning can carry out the same job as Nitrogen fixing bacteria Nutrition in Humans Carbohydrates: Elements- C, H, O Functions: Energy Structural (Cellulose Cell Wall in Plants) Types: 1. Monosaccharides – Simple Sugars 2. Disaccharides – Complex Sugars 3. Polysaccharides Monosaccharides Properties: Sweet tasting, white crystals, soluble in water. Function: Quick energy supply TEST – BENEDICTS SOLUTION 85°C = BLUE TO BRICK RED Formula: C6H12O6 (Ratio 1:2:1) E.g. Fructose, Glucose, Galactose, Ribose Disaccharides Properties: Sweet tasting (not as much as monosaccharides), white crystals, soluble in water. Function: Energy Supply Formula: C12H22O11 (One H2O molecule taken from the monosaccharide and then doubled) E.g. Lactose – Glucose + Galactose Sucrose – Glucose + Fructose Maltose – Glucose + Glucose Polysaccharides Properties: Insoluble, tasteless, large molecules Functions: Food store, Structural Carbohydrates Formula: C6H12O6 x 3000 E.g. Cellulose – Straight, no branches, structural cell. Glycogen – Coiled molecules, more branches, food store in ANIMALS, stored in muscle and liver. Starch – Coiled molecules, branches, food store in PLANTS. TEST – IODINE = BROWN TO BLUE/BLACK Cellulose Glycogen Starch Proteins Elements – C, H, O, N (P, S) Structure – AMINO ACID Side chain one of 20 different types. A total of 20 naturally occurring Amino Acids: Essential (have to come from diet, 8 types) Non-essential (produced by the body) Chains of Amino Acids joined together form 100 to 10,000 units long Examples: Insulin – regulates sugar levels Collagen – holds the skin in place Tendon – holds muscle to bone Muscle – for movement Enzymes – speeds up/ controls reactions Haemoglobin – carries Oxygen in the blood TEST – Biuret A + B = BLUE to PURPLE (lilac) Lipids/Fats Elements – C, H, O Properties – High-energy store/content, insoluble in water, less dense than water Formula/structure: 2 parts – Glycerol x 1 Fatty Acid x3 Soluble Hydrophilic Insoluble Hydrophobic Types 1. Saturated – Animal Fats 2. Unsaturated – Plant Fats Functions: Energy Store Thermal Insulation Electrical Insulation Mechanical Insulation Buoyancy TEST – Dissolve in Ethanol, then water = CLOUDY WHITE EMULSION ENZYMES Enzymes are biological catalysts that speed up chemical reactions. A type of protein How enzymes work: Lock and key hypothesis N.B Enzymes are specific and only work with one particular substrate. Types of Enzymes: Class Cardohydrase Example Amylase Amylase Maltase Protease Pepsin Trypsin Peptidases Lipase Lipase Digestive Action Starch to Maltose Starch to Maltose Maltose to Glucose Source Salivary Gland Pancreas Wall of the Small Intestine Protein to Peptide Stomach Wall Protein to Peptide Pancreas Peptides to Amino Wall of the Acids Small Intestine Lipids to Glycerol Pancreas and Fatty Acids Where it Acts Mouth Small Intestine Small Intestine Stomach Small Intestine Small Intestine Small Intestine Factors Affecting Enzymes: Temperature: When heated to optimum temperature rate of reaction is fastest. At colder temperature activity stops and when too hot the enzyme denatures. Concentration of Enzyme/substrate pH: Every enzyme has an optimum pH A BALANCED DIET A diet that provides everything the body needs should include: Carbohydrates Protein Lipids Vitamins Minerals Water Fibre Carbohydrates: Sources – Potato, bread, pasta, cereal Function – High-energy source Protein: Sources – Red meat, nuts, beans Function – Muscle repair and growth Lipids: Sources - Butter, milk, cheese, chocolate Function – Insulation, energy store Vitamins: A o Carrots, liver, butter o Night Vision C o Citrus Fruit, vegetables o Formation of connective tissue D o Ultra-Violet light o Bone and tooth building Minerals Iron o Liver, beef, vegetables o Haemoglobin in red blood cells Calcium o Milk, cheese o Bones, teeth and body fluids Water Source – Everything Function – Allows chemical reactions to take place in the body, composes 70% of our body Fibre Source – Wholegrain cereal Function – Maintains a healthy digestive system What a lack of a balanced diet can cause: Obesity – eating too much Anorexia – eating too little Deficiency Diseases – Scurvy, stunted growth, rickets Average Calorie Intakes in KJ New-born baby – 2000 Girl Aged 15-17 – 9000 Male Office Worker – 10,500 Manual Labourer – 15,000 Pregnant Woman – 10,000 THE DIGESTIVE SYSTEM Mouth Food is chewed and salivary glands release amylase and begin the breakdown of starch Oesophagus The tube connecting the mouth to the stomach where food is passed along through peristalsis Stomach Pummels (mechanically breaks down) the food. Releases Hydrochloric Acid to kill bacteria and create optimum acidic conditions of enzymes such as protease, which is released from the wall of the stomach to break down protein. Small Intestine Most of the digestion happens here. Proteases, carbohydrases and lipases are all released here. All the products of digestion are absorbed here across the surface of the small intestine into the blood. Villus Products of digestion are absorbed across the surface of the villus Amino acids, sugars pass into the capillary network by active transport Fatty acids and glycerol pass into the lacteal and are transported via the lymphatic system. Adaptations to absorb food efficiently Short diffusion distance due to very thin wall Microvilli and shape give a very large surface area Well connected to capillaries and lacteals so have a good blood supply and nutrients can be carried away easily. Pancreas Secretes enzymes (proteases, carbohydrases, lipases) and other proteins such as Insulin. Liver Produces Bile: Emulsifies fats (breaks them down into smaller droplets creating a larger surface area and more available substrate for the enzymes) Neutralises Acid for the stomach Creates optimum pH for the pancreatic digestive enzymes Gall Bladder Where the bile is stored Large Intestine Absorbs excess water. Rectum is where undigested food is stored before it is egested out of the anus. Digestive Processes Ingestion – Consumption of a substance Digestion – Breaking down of foods into nutrients that can be taken into the blood stream Absorption – Uptake of substances into the blood stream Assimilation – Conversion of nutrients into a form usable by the body Egestion – Discharging of undigested food from the body PERISTALSIS The movement of food along the gut is Peristalsis: Layers of circular and longitudinal muscles push food bolus through the gut via waves of contraction and relaxation. RESPIRATION Respiration is the process of releasing energy from food. There are two types; aerobic and anaerobic. Aerobic: Glucose + Oxygen Carbon Dioxide + Water (+ ENERGY) In Animals: Using Glucose in this equation is essential to humans as both the heart and the brain can only use glucose as fuel. To prevent cell damage from excess energy a ‘energy-rich molecule’ Adenosine Triphosphate (ATP) is made. In Plants Plants respire just as animals do using it to release energy from photosynthesis Plants respire just as animals do using it to release energy made in photosynthesis. Oxygen is obtained by diffusion of air through the stomata. Anaerobic Respiration This differs between plants and animals: In Animals: Glucose Lactic Acid (+Energy) C6H12O6 2 C3H6O3 When our muscles need energy and we cannot obtain enough oxygen to aerobically respire our body uses anaerobic respiration to generate energy. Less energy is produced than in aerobic respiration Lactic Acid is highly toxic so anaerobic respiration can only continue for a short while Once exercise has finished the lactic acid must be converted back to glucose using Oxygen; the is know as PAYING BACK THE OXYGEN DEBT In Plants: Glucose Ethanol (+Energy) C6H12O6 2 C2H5OH + 2CO2 Plants perform this if the roots of a plant get waterlogged and oxygen supply is low. Less energy is produced than in aerobic respiration Ethanol is poisonous so must be converted back like in animals using oxygen This is also known as FERMENTATION - process carried out by yeast and bacteria. Gas Exchange in Humans Inhalation External INTERCOSTAL muscles contract Rib cage moves UP and OUT Diaphragm CONTRACTS and FLATTENS Volume of chest cavity increases so pressure decreases Air is pulled in from the outside Exhalation External INTERCOSTAL muscles relax (internal intercostal muscles may contract for forced exhalation) Rib cage moves DOWN and IN Diaphragm RELAXES and CURVES UPWARDS Volume of chest cavity decreases so pressure increases Air is forced out The air enters the lungs down the trachea, which branches into the right and left bronchi. Each bronchus then divides further into bronchioles. After about 20 branchings you reach the air sacs, the alveoli. Each alveolus has a thin layer of epithelial cells separating the air from blood capillaries - a bit like the villi in the digestive system. Oxygen molecules diffuse from the alveoli into the blood stream, where there is a lower concentration of oxygen. The carbon dioxide diffuses the other way, from the high concentration in the blood to the alveoli. Adaptations for gas exchange: 1. Huge total surface area for gas exchange 2. Thin for rapid diffusion 3. Moist surface for gas to dissolve in during diffusion. 4. Excellent blood supply for gas transport and to maintain a good diffusion gradient Consequences of Smoking to the Lungs and Circulatory System: Tar from smoke paralyses ciliated cells, which normally sweep germs upwards in mucus. Germs therefore remain and cause infections such as Bronchitis. Tar causes Emphysema: breakdown of the alveoli and reduces surface area for oxygen absorption. CO causes 15% of haemoglobin to become carbonoxyhaemoglobin which is unable to pick up oxygen so reduces athletic ability CO in smoke promotes ATHEROSCLEROSIS (laying down fatty deposits in the arteries) insufficient oxygen reaching the heart via coronary arteries may result in a heart attack. Insufficient oxygen to limbs may result in amputation owing to gangrene. Transport in Humans Blood: Made up of 4 main components: Plasma Red Blood Cells White Blood Cells Platelets Plasma – Transports dissolved substances around the blood including: Hormones Nutrients: water, glucose, amino acids, minerals and vitamins. Waste substances: carbon dioxide and urea. Red Blood Cells – Transport oxygen around the body and are adapted in several ways to do this: Have no nucleus so extra space for oxygen Are a BICONCAVE DISC maximizing surface area for oxygen diffusion Contain a protein called HAEMOGLOBIN – combines with oxygen to form OXYHAEMOGLOBIN to carry it round the body to respiring muscles. Tiny so they can fit in small capillaries and carry oxygen everywhere in the body There are huge numbers of red blood cells White Blood Cells – Part of the body’s immune system that identify and destroy pathogens in the blood. There are two types: Phagocytes Lymphocytes Phagocytes – These perform phagocytosis once the have spotted a pathogen by its surface antigens: The phagocyte detects the presence of a pathogen, puts out projections called PSEUDOPODIA that engulf the pathogen into a small vacuole where digestive enzymes are secreted and the pathogen is digested becoming nutrients for the cell. (The granular appearance of the cells is due to this breaking down of the pathogen inside the cell) Lymphocytes – These give a specific response to a particular pathogen. An activated lymphocyte divides many times and produces proteins called ANTIBODIES which fight disease in one of the following ways: Make bacteria stick together so that phagocytes can ingest them more easily Making bacterial cells burst open Sticking to the pathogen so the phagocyte can recognise it Neutralising toxins produced by the pathogen Some lymphocytes remain in the blood as MEMORY CELLS making that person immune to theta pathogen in the future. Vaccination and Immunisation: Vaccination aims to make a person immune to a disease even though they have never suffered or been ill with it. Once a person has been vaccinated memory cells will work against that particular pathogen are produced, this meaning that if a pathogen re-enters the body the immune system can respond so quickly that no symptoms occur. Vaccination works by injecting people with one of the following: A weakened strain of the actual organism (T.B, polio and measles) Dead microorganisms (Typhoid and whooping cough) Modified toxins of the bacteria (Tetanus and diphtheria) Antigens (Influenza) Harmless bacteria genetically engineered to carry the antigens of a disease carrying microorganism (Hepatitis B) Platelets – These clot the blood to avoid excess bleeding due to an injury and prevent harmful pathogens entering the body. Injury – Platelets are activated and release clotting factors – These convert soluble FIBROGEN into insoluble FIBRIN – Cells are trapped in these threads like a fine net forming a CLOT or THROMBOSIS by stopping the flow of blood. THE HEART N.B 1. The bicuspid and tricuspid valve are collectively know as the ATRIOVENTRICULAR VALVES 2. The left ventricle has a thicker wall than the right as the aorta has to carry blood all the way round the body requiring a higher pressure whereas the right ventricle only pumps to the lungs. 3. The function of the valves is to prevent BACKFLOW of blood and the tendons are there to stop the valves inverting. The Cardiac Cycle – Blood is moved through the heart by a series of contractions and relaxations of cardiac muscle in the walls of the four chambers. 1. Blood enters the atria. It cannot yet pass into the ventricles as the bicuspid and tricuspid valves are closed. 2. The walls of the atria contract. This raises the pressure of the blood in the atria which forces open the atrio-ventricular valves. Blood passes through these valves into the ventricles. 3. When the ventricles are full, they contract. This increases the pressure of the blood in the ventricles, which closes the atrio-ventricular valves, which prevents the backflow of blood into the atria. 4. The ventricles continue to contract and increase pressure. This forces the semi-lunar valves open at the base of the aorta and the pulmonary artery. Blood is ejected into these two arteries. The aorta carrying blood all the way around the body and the pulmonary artery to the lungs. 5. As the ventricles empty, higher pressure in the pulmonary artery and the aorta causes the semi-lunar valves to close. The cycle then begins again as the atria fill with blood. N.B Contractions is known as SYSTOLE and Relaxation is known as DIASTOLE Heart Rate: This is usually about 70 beats per minute. If the body is respiring quickly like when we exercise our heart rate increases – The increased CO2 level is detected by the medulla of the brain and the cardiac centre instructs the heart to speed up or slow down via accelerator or decelerator nerves. These communicate with a ‘pacemaker’ on the wall of the right atrium, which controls the heart rate though, electrical impulses. When adrenaline is produced by the adrenal gland it prepares the body for ‘fight or flight’. The heart pumps faster as blood is diverted to muscles to provide them with oxygen for respiration so they can move quickly Coronary Heart Disease Caused by a blood clot in the coronary arteries that supply blood to the heart. Clotting is more likely if you have lots of atheroma (fatty deposits) in your arteries. Atheromas are more likely to develop if you have RISK FACTORS in your life: Stress Lack of exercise Smoking Too much saturated fat in the diet; from animal products as this causes HIGH CHOLESTEROL Hereditary factors i.e. you have a predisposition to heart disease in your genes High Blood pressure which can be cause by too much salt in the diet Reversing what you can of these risk factors makes blockage much less likely. Circulatory System Arteries, capillaries and veins Factor Function Nature of Blood Flow Structure and Thickness of wall Width of vessel lumen Valves? Reasons for structure Arteries Carry oxygenated blood away from the heart High pressure, fast, pulsatile Capillaries Carry blood close to every cell Very thick, elastic (STRETCH + RECOIL), muscle Narrow Thin (1 cell thick), fenestrations Veins Carry deoxygenated blood back to the heart Slow (faster than capillaries), low pressure Thin, little muscle or elastic Extremely tiny Wide No Withstand, maintain high blood pressure No Short diffusion distance Yes Same blood volume as arteries at lower pressure Slow, dropping pressure Plant Nutrition PHOTOSYTHESIS Photosynthesis is essentially the opposite to respiration as it uses Carbon Dioxide and Water to create Glucose and Oxygen. It is essential to life as it converts light energy to chemical energy. Its is affected by: Light intensity Carbon Dioxide concentration Temperature Light intensity Without enough light, a plant cannot photosynthesise very quickly, even if there is plenty of water and carbon dioxide. Increasing the light intensity will boost the speed of photosynthesis. Temperature If it gets too cold, the rate of photosynthesis will decrease. Plants cannot photosynthesise if it gets too hot. Leaf Structure Carbon dioxide concentration Sometimes photosynthesis is limited by the concentration of carbon dioxide in the air. Even if there is plenty of light, a plant cannot photosynthesise if there is insufficient carbon dioxide. Adaption Purpose Large surface area To absorb more light Thin Short distance for carbon dioxide to diffuse into leaf cells Chlorophyll Absorbs sunlight to transfer energy into chemicals Network of veins To support the leaf and transport water and carbohydrates Stomata Allow carbon dioxide to diffuse into the leaf Adaption Purpose Epidermis is thin and transparent To allow more light to reach the palisade cells Thin cuticle made of wax To protect the leaf without blocking out light Palisade cell layer at top of leaf To absorb more light Spongy layer Air spaces allow carbon dioxide to diffuse through the leaf, and increase the surface area Palisade cells contain many chloroplasts To absorb all the available light Plants need to take in a number of elements to stay alive. The most important are: Carbon Hydrogen Oxygen Plants get hydrogen and oxygen from water in the soil, and carbon and oxygen from carbon dioxide and oxygen in the atmosphere. Water and carbon dioxide are used to synthesise food during photosynthesis. Oxygen is used to release energy from food during respiration. In addition to these three elements, plants need a number of minerals for healthy growth. These are absorbed through the roots as mineral ions dissolved in the soil water. Two important mineral ions needed by plants are: Nitrate - for making amino acids, which are needed to make proteins Magnesium - for making chlorophyll If a plant does not get enough minerals, its growth will be poor. It will suffer from deficiency symptoms: Deficient in nitrate - it will suffer from stunted growth Deficient in magnesium - it's leaves will turn yellow Plants obtain water and minerals by absorbing water through the root hair cells through osmosis and active transport. Plant Gas Exchange Cont. Plants carry out both respiration and photosynthesis. PSN fluctuates greatly throughout the course of the day but respiration remains fairly constant. Stomata are pores in the leaf that let gases in and out by diffusion. When hot they close and when they have a good water supply remain open. They are open and closed by guard cells Leaf adaptation for Gas Exchange – Stomata: free unhindered diffusion of CO2 into leaf and O2 out of leaf. High Stomata Density: the more stomata the greater the amount of gas exchanged. Thin: the shorter the distance the faster the rate of diffusion of CO2 and O2. Great Surface Area: the greater the surface area the greater the gas exchanged. Flat: maintains the highest possible concentration difference for fastest diffusion. Internal Air Spaces: diffusion of CO2 and O2 is much faster in air than in water. Moist Internal Surface: required for the absorption and release of gas from the leaf cells. PLANT TRANSPORT Plants have two different types of 'transport' tissue. Xylem transports water and solutes from the roots to the leaves, phloem transports food from the leaves to the rest of the plant. Both of these systems are rows of cells that make continuous tubes running the full length of the plant. Xylem cells have extra reinforcement in their cell walls, and this helps to support the weight of the plant. For this reason, the transport systems are arranged differently in root and stem – in the root it has to resist forces that could pull the plant out of the ground. In the stem it has to resist compression and bending forces caused by the weight of the plant and the wind. Stem – the xylem and phloem are arranged in bundles near the edge of the stem to resist compression and bending forces. Comparison of xylem and phloem Tissue Process What is moved Structure Xylem Transpiration Moves water and minerals from roots to Columns of hollow, leaves dead reinforced cells Phloem Translocation Moves food substances (sucrose and Columns of living cells amino acids) between the leaves and the rest of plant Transpiration The transpiration stream keeps the upright with cells turgid. Water taken up by the roots of a plant is transported through a plant to the leaves and lost into the air. The stages of the process are: Water enters root hair cells by osmosis. The root hair cell is hypertonic to the surrounding soil water. This means that it has a lower water molecule concentration. Water then moves from cell to cell through the root cortex by osmosis along a concentration gradient; this means that each cell is hypertonic to the one before it. In the centre of the root the water enters the xylem vessels. Water may move by diffusion through the cell walls and intercellular spaces. In the leaves, water molecules leave the xylem vessels and move from cell to cell. They move through the spongy mesophyll layer by osmosis along a concentration gradient. Water then evaporates into spaces behind the stomata and diffuses through the stomata into the surrounding air. Water rises from the roots to the leaves through the xylem vessels because of two properties of water molecules: Adhesion Water rises in the narrow vessels partly because water molecules are attracted to the walls of the vessels. Cohesion Water molecules are attracted to each other, and as water evaporates from the leaves columns of water are drawn up through the xylem vessels. The loss of water from the leaves of a plant is called transpiration, and the resulting flow of water through the plant is called the transpiration stream. The transpiration stream is important because: It carries water for photosynthesis to the palisade cells in the leaves The water carries essential mineral salts in solution Evaporation from the leaves has a cooling effect Factors that affect transpiration rate Factor Description Explanation Light In bright light transpiration increases The stomata (openings in the leaf) open wider to allow more carbon dioxide into the leaf for photosynthesis Temperature Transpiration is faster in higher temperatures Evaporation and diffusion are faster at higher temperatures Wind Transpiration is faster in windy conditions Water vapour is removed quickly by air movement, speeding up diffusion of more water vapour out of the leaf Humidity Transpiration is slower in Diffusion of water vapour out of the leaf slows humid conditions down if the leaf is already surrounded by moist air Plant Coordination and Response Plants do not move from place to place but others parts of the plant such as the stem and flowers respond to stimuli by twisting, turning, opening and closing – what are called TROPISMS or NATISMS respectively There are many different types: Negative or Positive Phototropism/Photonastic Geotropism Hydrotropism Thigmotropism Hydrotropism Auxin is a chemical messenger that in the stem and root of a plant causes it to grow in a particular direction. In the shoot it causes the shoot to bend towards the source of light. The Auxin accumulates on the dark side causing cells on that side to elongate and bend towards the light. Plant Reproduction There are two types of reproduction Asexual and Sexual. Plants can do both naturally and artificially. Asexual Reproduction Bulbs – Potatoes, daffodils Cuttings – Geranium Runners – Strawberry Sexual Reproduction The production of an organism from two parents making use of their sex cells. Advantages There is variation in the offspring New varieties are produced Disadvantages Two parents are always needed Growth of a plant from seed is vegetative propagation In plants seeds are produced and dispersed away from the parent plant, thus reducing the competition Plant Structure: Functions of different parts of a flower: Stamens make the male gamete, which is stored in pollen grains. Carpel, the female part of a flower, makes the female gametes. These are stored or are present in ovules. Male gametes from the pollen grains travel (get dispersed) and then pollinate and fertilise the female gametes in the ovules. The fertilised ovules grow to become seeds. Pollination: Cross Pollination Self Pollination Transfer of pollens from the anther of a The pollen is transferred to the stigma of the plant to stigma of another plant of the same same flower species Needs agent for pollinations; water, insects, Less chances of failure of pollination birds or man Results in healthy and strong offspring Purity of race is maintained Results in production of large number of Avoids wastage of pollen grain seeds New varieties with useful characters are Flowers do not need to be large and showy produced Cross pollination is used for producing new Continuation of self pollination results in kinds of vegetables and fruits weak progeny A plant requires a lot of energy and food New varieties and species of plants are not material to bring about pollination produced Differences: Feature of Flower Position of Stamens Position of Stigma Type of Stigma Size of Petals Colour of Petals Nectaries Pollen Grains Insect Pollinated Enclosed within the flower so that the insect must make contact Enclosed within the flower so that the insect must make contact Sticky so pollen grains attach from insects Large to attract insects Brightly coloured Wind Pollinated Exposed so pollen can be blown away easily Exposed to catch pollen blowing in the wind Feathery to catch pollen blowing in the wind Small Usually green but not brightly coloured Present as nectar is a reward for the Absent insects Larger, sticky grains to stick to the Smaller, smooth inflated insects body grains to carry in the wind 1 - filament 2 - anther 3 - stigma 4 - style 5 - petal 6 - ovary 7 - sepal 8 - flower stalk 9 - stamen 10 - carpel 11 - perianth Growth of pollen tube and fertilisation 1. Pollen grain of the same species reach the stigma 2. Pollen tubes are formed, triggered by sugary solution on the stigma 3. The pollen tube grows down through the style and reaches the ovule 4. Pollen tube contains two male gametes 5. The tube pushes its way through the ovary wall and through the micropyle of the ovule 6. Fertilisation occurs Formation of seed and fruit: After fertilisation occurs the sepals, petals, stamens, styles and the stigma of the flower wither and fall off The ovary grows rapidly and forms a seed The seed now contains the embryo (tiny root radicle, shoot plumule) and the cotyledons. The wall of the ovule thickens to form the seed coat/testa The wall of the ovary grows to form the fruit. It may be fleshy or dry A fruit protects and distributes the seed Seed dispersal can occur by wind or animal: Wind: seeds bear parachutes like wing, fine hairs, are very light Animals: belong to succulent fruits or hooked fruits Factors that affect Germination: Water – needed to activate enzymes for converting soluble food stores in the cotyledons to soluble food, which can be used for growth by the baby plant. Oxygen – needed for respiration as soon as growth process begins and required for energy to mobilise the chemical changes. Warmth – enzymes present in the seed get activated and work best at optimum temperature (20-40°C), which triggers growth. Sexual Reproduction in Humans 1. Testes: males gonads that produce sperm 2. Scrotum: a sac that holds testis outside the body 3. Epididymis: a mass of tubes in which sperms are stored 4. Seminal vesicle: adds fluid and nutrients to sperm to form semen 5. Sperm duct: muscular tube which links the testis to the urethra to allow the passage of semen containing sperm 6. Prostate gland: adds fluid and nutrients to form semen 7. Urethra: to pass semen containing sperm through the penis also carries urine from the bladder at times 8. Penis: organ to transfer sperm to the vagina Secondary sexual characteristics: broadening of shoulders. Growth of body hair, deepening of voice, increased development of musculature, penis becomes larger, and testes start to produce sperm 1. Ovary: contains follicles in which ova are produced 2. Oviduct: carries the ovum to the uterus. This is the fallopian tube and the site of fertilisation 3. Funnel of oviduct: direct and extends from the ovary to oviduct 4. Uterus: where the foetus develops 5. Cervix: a ring of muscles that separates the vagina from the uterus 6. Vagina: receives male penis during intercourse. Sperm is deposited here. 7. Urethra: carries urine form the bladder 8. Puberty: period when reproductive organs become functional Secondary sexual characteristics: breasts grow, nipples enlarge, hair develops under arms and in pubic area, uterus and vagina become larger, ovaries start to release eggs and menstruation begins and hips become wider. Menstruation The periodic discharge of blood, mucus and epithelial cells from the lining of the uterus through the vaginal opening is called menstruation. It happens every 28 days and lasts for 45 days. Ovum is produced every 28th day Ovum cannot be fertilised after 48 hours Sperms live for 48 hours Fertilisation: Sperm reaching the fallopian tube has a chance to meet ovum It penetrates the ovum and the zygote is formed The zygote rapidly divides by mitosis as it travels down through the fallopian tube forming a small ball of many cells called a blastula This ball of cells becomes embedded or implanted in the mucosa of the uterus Development of foetus: In humans, the egg is without reserve nutrients The embryo gets nutrients and oxygen from the mother until birth The embryo develops three embryonic membranes that surround it Amniotic sac – membrane formed from the cell of the embryo, which contains amniotic fluid and encloses the developing foetus and prevents entry of bacteria After implantation an organic connection is established between the extra embryonic membranes of the embryo and the uterine wall of the mother known as the placenta. The functions of the placenta are: 1. Receiving dissolved food substances from the mothers blood 2. Receiving oxygen from the mothers blood 3. Excreting CO2, nitrogenous waste and urea 4. Serving as an important but temporary endocrine gland Antenatal care: A balanced diet: Protein for growth, calcium for skeleton, iron for RBC development of skeleton, vitamin c for good bones and skin, carbohydrates for fats and energy, vitamins and minerals to prevent deficiency Process of Birth: 1. Labour triggered by hormone oxytocin 2. Muscular walls of the uterus start to contract 3. The pressure breaks the amniotic sac 4. Contractions become more frequent pushing the baby down towards the cervix 5. Cervix becomes dilated allowing the baby to pass 6. The vagina stretches for the baby to pass 7. The baby is still attached to the placenta by the umbilical cord 8. Placenta breaks away from the uterine wall and passes out (after birth) 9. Umbilical cord is cut and tied Birth Control: 1. Mechanical Methods a. Condom b. Femidom c. Diaphragm d. IUD (intrauterine device) 2. Natural Methods a. Withdrawal b. Abstinence c. Rhythm 3. Chemical Methods a. The pill b. Spermicidal 4. Surgical Methods a. Vasectomy b. Laparotomy/ tubectomy Homeostasis: The maintenance of a constant internal environment Things that are kept constant: Level of sugar Temperature: Thermoregulation Water level: Osmoregulation Level of salt Waste products like urea cannot exceed a certain level Blood pH would become too low if levels of CO2 or lactic acid get too high Enzymes are adversely affected by changes in pH or temperature Changes in a body system are detected by RECEPTORS and EFFECTORS bring about the necessary corrections. Sugar (HYPOGLYCAEMIC AND HYPERGLYCAEMIC) Two hormones control levels of glucose: Insulin and Glucagon Thermoregulation: Mammals and birds have a constantly warm body temperature regardless of their surroundings and are therefore described as being HOMEOTHERMIC. As their body heat is primarily generated by their own chemical reactions, they are also described as ENDOTHERMS. In contrast cold-blooded animals get body heat from the sun – ECTOTHERMIC. Mammals including humans have a THERMOREGULATORY CENTRE in the HYPOTHALAMUS of the brain, which monitors blood temperature. This will regulate body temperature by sending nerve impulses that bring about physical changes such as sweating. There are also temperature receptors in the skin that inform the hypothalamus of changes in environmental temperature. HOT 1. When our skin hot it acts to increase heat loss 2. The hair erector muscle relax and make the hair lies flat against the skin trapping less and more heat is lost by radiation 3. Sweat glands make more sweat which evaporates from the surface taking away heat 4. VASODILATION blood vessels widen and increases heat loss by radiation COLD 1. Our skin acts to decrease heat loss when it is cold 2. The hair erector muscles contract and make the hair stand more upright. This traps warm still air close to the surface of the skin. Less heat is lost by radiation. 3. Sweat glands stop making sweat 4. VASOCONSTRICTION blood vessels near the skin get narrower and less heat is lost by radiation Osmoregulation This is controlled by the anti-diuretic hormone (ADH) ADH works by making the collecting duct of the nephron in the kidney more permeable to water. If there is too high concentration of ADH in the blood the collecting ducts will become more permeable and more water will move back from the urine to the blood stream; when there is too little water in the blood. If there is too much water in the blood then less ADH is released. This is an example of NEGATIVE FEEDBACK. Excretion: is removal from the body of the waste products of metabolism The Kidney: The Nephron: N.B Kidneys lie at the back of the abdomen Kidneys require a unusually high blood supply to maintain high blood pressure Women suffer from bladder infections more because the urethra is shorter and close to the anus making a greater chance of transfer of bacteria We have two separate kidneys in case one is damaged: If a kidney is damaged then people need a transplant or dialysis. Dialysis: a machine that when connected to the body removes urea and other toxins. Transplants: are considered easy for kidneys due to the fact that kidneys are only connected via the renal artery and vein Arteriole: takes blood into the capillaries of the glomerulus. The arteriole is wider on the way in than on the way out the blood pressure is very high Glomerulus: a knot of capillaries Bowman’s Capsule: water and small molecules (amino acids, salts, urea and glucose) enter here by ULTRAFILTRATION First Coiled Tubule: Useful molecules such as glucose and amino acids and water are SELECTIVELY REABSORBED here. Loop of Henlé: This concentrates the filtrate and allows more reabsorption of water. Second Coiled Tubule: more reabsorption Collecting Duct: Is where the exact water content of the filtrate is determined by the hormone ADH and where the finished filtrate, urine, emerges. N.B Excretion can also occur as sweating through pores of the skin and exhalation of Carbon Dioxide from the lungs The Endocrine System Produces and secretes chemical messengers called hormones. Gland Pituitary Hormone Produced 1.ADH 2.LH 3.FSH Thyroid Adrenal Pancreas Thyroxin Adrenaline Insulin Ovary 1.Oestrogen 2.Progesterone Testes Testosterone Action 1.Water balance 2.Ovulation, progesterone production 3.Growth of follicle Controls metabolic rate Prepares the body for action Control of blood glucose level 1.Controls puberty and menstrual cycle 2.Maintains pregnancy Controls puberty in male Effects of Adrenaline: Target Organ Effects of Adrenaline Biological advantages Heart Beats faster Pumps more blood to get oxygen and glucose Breathing center of the Faster and deeper More oxygen into brain breathing blood and carbon dioxide out of blood Arterioles of skin Vasoconstriction Blood getting where it is most needed Arterioles of digestive Constricts them Blood getting where system it is most needed Muscles of alimentary Relax To save energy canal Muscles of body Tenses them Ready for action Liver Conversion of Used for respiration glycogen to glucose Fat deposits Fats to fatty acids Used for respiration Effect Thumping heart Panting Person goes paler Dry mouth ‘Hollow’ stomach feeling Tense feeling No sensation No sensation