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Lab Biology NJBCT Review Guide Test Dates: Tuesday, May 21st and Wednesday, May 22nd Format: 60 Multiple Choice and 2 Performance Assessment Questions Unit 1: Scientific and Laboratory Procedures (Chapter 1) What are the goals of science? To investigate, predict and explain events in the natural world Explain the difference between quantitative and qualitative research. Quantitative expressed as numbers and can be measured and qualitative is descriptive What is the difference between an inference and a hypothesis? Inference: a logical interpretation based on prior knowledge Hypothesis: proposed explanation for a set of observations or possible answer to a scientific question What is a theory? A well-tested explanation that unifies a broad range of observations List the steps of the scientific method. Question, hypothesis, experiment, observe, analyze and share results List and explain the two types of variables you will find in a scientific experiment. Dependent variable (aka: responding variable)- factor you want to observechanges in response to the independent variable Independent variable (aka: manipulated variable)- factor that you purposefully change What is the purpose of a control variable in an experiment? It is purposefully kept the same- used to compare the dependent variable to List the 8 characteristics that all living things have in common. 1. Made of cells 2. Reproduce 3. Universal genetic code (DNA) 4. Grow and develop 5. Obtain and use materials for energy 6. Respond to environment 7. Maintain stable conditions (homeostasis) 8. Evolve What is the difference between a compound microscope and an electron microscope? Compound uses light and electron uses a beam of electrons to magnify an image/object List the units used in the metric system when measuring: length, volume, mass and temperature. Length: use a ruler (meters) Volume: use a graduated cylinder (liters) Mass: use a triple beam balance/scale (gram) Temperature: use a thermometer (Celcius) What tools might you use to measure each of the above factors? See above List some common safety rules and procedures. Wear goggles/aprons, do not eat in lab, tie hair back, pay attention, wash hands, no horseplay, etc. Unit 2: Matter, Energy and Organization of Living Things (Chapters 2, 7, 8 and 9) (Part A) What is all matter made up of? atoms List and explain the 2 types of chemical bonds. Ionic: electrons transferred from one atom to another Covalent: electrons are shared between atoms What is a monomer? What is a polymer? Monomer: small individual molecules Polymer: compound formed by joining monomers Identify the structure (monomer) and roles of each of the 4 macromolecules. Carbohydrates- made up of monosaccharides-> polysaccharides Lipids- made up of glycerol molecules and fatty acid chains Nucleic acids- made up of nucleotides Proteins- made up of amino acids What are some important properties of water? Density- ice is less dense that liquid water Polarity- uneven distribution of electrons- gives slightly + and slightly - sides Adhesion- ability of water to “stick” to other surfaces Cohesion- ability to “stick together” High specific heat- takes long time to heat up/cool down Explain the difference between a mixture, a solution and a suspension. Mixture: 2 or more elements physically but NOT chemically combined Solution: when the molecules of a mixture are evenly distributed Suspension: mixture of water and non-dissolvable material What does the pH scale show us? What numbers indicate a base? An acid? Concentration of H+ ions in a solution- acids= 1-7 and bases= 8-14 What does the basic formula of a chemical reaction show? Reactants (what goes in) on the left side and products (what comes out) on the right- the arrow signifies the reaction What is activation energy? Energy needed to get a reaction started What is an enzyme? Type of protein that works as a biological catalyst and lowers the activation energy of a reaction so it can happen quicker/faster Explain the Cell Theory. 1. All living things are made of cells 2. Cells are the basic unit of structure and function in living things 3. New cells come from older cells Identify the major organelles and their function in a cell. Nucleus- control center of cell- contains DNA Cytoplasm- area outside the nucleus- holds all the organelles in place and helps transport materials Ribosomes- make proteins Endoplasmic reticulum- conveyor belt for cell products leaving the cellrough ER: transports proteins; smooth ER: makes membrane lipids Golgi apparatus- modifies, sorts and packages proteins Vacuoles- storage Mitochondria- takes chemical energy from food and turns it into ATP Chloroplasts- (plants only) take light energy and turn it into chemical energy for the cell Cell membrane- controls what goes in/out of cell Cell wall (plants only)- structural support Identify how cell specialization involves the relationship between structure and function. Different types of cells have different structures because the perform different functions (ex: blood cells shaped the way they are so they can carry oxygen) Unit 2: Matter, Energy and Organization of Living Things (Chapters 2, 7, 8 and 9) (Part B) How are diffusion and osmosis similar? Both go from high concentration to low- osmosis is diffusion of water What is facilitated diffusion? Diffusion across membrane with the “facilitation” (help) of protein channels What is active transport? Moves materials across membrane against the concentration gradient from low to high- requires energy to work- examples include protein channels, exocytosis (getting things out) and endocytosis (taking things in) What is the difference between autotrophs and heterotrophs? Autotroph: produces its own food (from chemicals or sunlight) Heterotroph: gets energy from consuming food What is ATP? What is ATP synthase? ATP is a chemical compound that living things use to store and release energy- ATP synthase is a protein that makes ATP What are the reactants of photosynthesis? What are the products? Reactants (in) CO2, water and light energy (sunlight) Products (out): sugar and O2 What is the role of a pigment? Absorb light energy from the sun What happens in the light dependent reactions? What happens in the Calvin Cycle? Light dependent reactions: use light and water to make ATP, NADPH and O2 Calvin Cycle: ATP and NADPH combine with CO2 to make sugars List a few environmental factors that influence the rate of photosynthesis. Amount of light and H2O available and the temperature What are the reactants of cellular respiration? What are the products? Reactants (in): sugar and O2 Products (out): CO2, water and ATP (energy) What happens in glycolysis? Sugar is split (1 glucose splits into 2 pyruvic acids) What is fermentation? Where cells can release energy from food without O2 present What is an electron transport chain? Why is it important? A series of proteins that convert ADP into ATP- important because it creates ATP Unit 3: Diversity and Biological Evolution (Chapters 15, 16, 17 and 18) (Part A) What is evolution? Change over time Explain natural selection (aka: survival of the fittest) Process by which those best suited/adapted to their environment reproduce and pass on their genes moat successfully- successful adaptations will increase your fitness Explain the three types of natural selection. 1. directional: one end of the curve has increased fitness 2. stabilizing: center of the curve has increased fitness 3. disruptive: both ends of the curve have increased fitness What is artificial selection? Selection by humans for the breeding of useful traits What is an adaptation? Why are these important? Inherited characteristic that increases an organisms chance of survival Explain descent with modification. Each living thing has descended (with modifications) from other species over time What are homologous body structures? What can they tell us? Structures that have different mature forms in different organisms but develop from the same embryonic tissue- they can be sued to tell how closely related two organisms are Explain what relative frequency means in a gene pool? Number of times an allele appears in a gene pool List and explain the two type of evolution without natural selection. 1. genetic drift: random change in alleles frequency occurring in a small population 2. founder effect: change in allele frequency due to the migration of a small subgroup of a population What is the Hardy-Weinberg Principle state? Allele frequencies will remain constant in a population (no evolution will occur) as long as there is random mating, large populations, no movement in/out of the population, no mutations and no natural selection (THIS DOES NOT USUALLY HAPPEN!) How are speciation and reproductive isolation related? Speciation occurs with reproductive isolation which is when two organisms become separated to the point where they can no longer breed with each other List and explain the three types of reproductive isolation. 1. behavioral: different mating behaviors 2. geographic: physical barriers separate 3. temporal: mate ad different times/seasons Unit 3: Diversity and Biological Evolution (Chapters 15, 16, 17 and 18) (Part B) Compare and contrast relative dating and radioactive dating of fossils? Relative dating: uses vertical order (older rocks at the bottom) Radioactive dating: uses half-life- it is more accurate What is the endosymbiotic theory? Eukaryotic organisms came from a symbiosis among prokaryotic organisms What is extinction? When an entire species dies out Explain adaptive radiation Give an example. When a group of species evolves into several different forms living in different ways (ex: Darwin’s finches) Explain convergent evolution. Give an example. Unrelated organisms develop similarities from living in similar ways or in similar environments (ex: penguins, sharks, dolphins all have swimming appendages) Explain coevolution. Give an example. When to species evolve together (ex: moth and orchid flower) Explain how punctuated equilibrium is different from Darwin’s idea of gradualism. Gradualism (Darwin) said everything evolves slowly but constantly and punctuated equilibrium says there are periods of rapid change What is taxonomy? What are the seven taxa (in order)? Classifying organisms and assigning each a universally accepted name Kingdom, phylum, class, order, family, genus, species What is binomial nomenclature? Classification system that assigns each organism a two-part (genus and species) scientific name What can a dichotomous key show us? What can a phylogenetic tree show us? Dichotomous key: uses observable characteristics to classify Phylogenetic trees show ancestral relationships List some defining characteristics for each of the six kingdoms. Eubacteria: prokaryotes Archaebacteria: live in harsh environments Protista: unicellular heterotrophs Fungi: decomposers Plantae: photosynthetic autotrophs Animalia: multicellular heterotrophs Unit 4: Reproduction and Heredity (Chapters 10, 11, 12 and 13) (Part A) List and explain the four phases of the cell cycle. G1 phase- growth S phase- chromosomes replicate G2 phase- more growth M phase- cell division What is a chromosome? Structure that contains the genetic information passed from generation to generation What are the two parts of cell division? Mitosis and meiosis List and explain the four phases of mitosis. Prophase- chromatin condenses, nuclear envelope disappears Metaphase- centrioles move out and spindle appears, chromosomes line up on metaphase plate Anaphase- sister chromatids pulled apart Telophase (Cytokinesis too)- cytoplasm divides equally, new nuclear envelopes form in each new cell What is cancer? Uncontrolled cell division What are alleles? Different forms of a gene What are gametes? Specialized haploid cells involved in sexual reproduction Explain cross breeding. Genetically crossing two different organisms What is the difference between dominant and recessive traits? Dominant traits show as long as there is at least one dominant allele presentrecessive trait only show if you have two recessive alleles What does a Punnett Square show? Used to predict the possible outcome for the offspring of 2 specific parents whose genotypes are known Contrast homozygous and heterozygous alleles. Homozygous: you have two of the same allele (ex: RR or rr) Heterozygous: you have two different alleles (ex: Rr) Explain the difference between genotype and phenotype. Genotype: genetic makeup (ex: Ff, FF or ff) Phenotype: physical trait (ex: freckles or no freckles) What is Mendel’s Law of Independent Assortment? Genes are inherited independently of one another, each time a new offspring is produced Unit 4: Reproduction and Heredity (Chapters 10, 11, 12 and 13) (Part B) Explain incomplete dominance. Give an example. One allele is not completely dominant over another (ex: pink flowers as a result of crossing red and white) Explain codominance. Give an example. Both alleles contribute to the phenotype (ex: chicken with both black and white feathers) Explain multiple alleles. Give an example. When there are more than just two alleles for a trait (ex: blood types) Explain polygenic traits. Give an example. When more than one gene controls a trait (ex: skin color) What is meiosis? What do you begin with and what do you end with? What is the difference between a diploid cell and a haploid cell? Diploid contains both sets of homologous chromosomes and haploid only has one set- haploid cells are your gametes Identify the structure of DNA. 5 carbon sugar, phosphate group and nitrogenous bases (adenine, cytosine, guanine and thymine)- the shape is a double helix or “twisted ladder” What is complimentary base pairing? A=T and C=G Identify the enzymes involved in DNA replication. Helicase: unzips the DNA strand DNA polymerase: forms the new DNA strand What is protein synthesis? List and explain the two main parts of this process. When DNA codes for proteins and “makes proteins”- two parts are transcription (turning DNA into RNA) and translation (using RNA to code for amino acids/proteins) What is a codon? Sequence of 3 nucleotides in mRNA that codes for a specific amino acid Identify the different types of gene and chromosomal mutations. Gene mutations = change in a single gene (ex: point) Chromosomal mutations = change in entire chromosome or a big piece of a chromosome (ex: deletion, duplication, inversion, translocation) What does gene expression mean? When a gene is “turned on” and you express that trait Unit 5: Environmental Systems and Interactions (Chapters 3, 4 and 19) What is homeostasis? Maintaining internal balance List the four major types of pathogens. Viruses, bacteria, protists and fungi How do bacteria replicate? 1. Binary fission (DNA replicates and organism splits in two) 2. Conjugation (a “bridge” allows genetic info to pass from one parent to another 3. Spores (haploid reproductive cells enclosed in a protective covering can withstand harsh conditions and become useful when conditions improve How are bacteria useful? Decomposers, can cycle nutrients through a system (nitrogen), found in human digestive systems to help digestion, human uses include food treatments, medical uses, etc. How may you treat bacteria? Sterilization (heat), disinfection (chemicals) or antibiotics How do viruses replicate? Lytic replication: virus enters host and continuously replicates until the host cell is full and bursts Lysogenic replication: viral DNA combines with host cell’s DNA How may you treat a virus? Vaccinations can be used to prevent Explain the roles that antigens, antibodies and WBCs have in the immune system? Antigens are part of pathogens that attack the body, antibodies are created by WBC’s to fight the antigens What is ecology? The study of interaction between organisms and their environment What is the difference between a producer and a consumer? Producers: use energy from environment (chemicals or light) to make their own food Consumers: get energy from the food they eat List and explain the five different types of consumers. 1. Herbivores: eat only plants 2. Carnivores: eat only meat 3. Omnivores: eat both plants and meat 4. Detritivores: eat detritus (dead decaying organic matter) 5. Decomposers: eat decaying organic matter and return nutrients to earth What do food chains/webs and ecological pyramids show us? How energy is transferred from one trophic level to the next- pyramids show the amount of energy contained in each trophic level- bottom is always the producers and always the largest population ******************************************************************************* Additional review questions NOT answered by lab groups this week (Chapter 4): Briefly explain the water cycle. Evaporation and transpiration bring water from earth into atmospherecondensation forms clouds- precipitation brings water back down to earth Why are the nitrogen and carbon cycles important to humans? Nitrogen makes up parts of amino acids- carbon makes up all of the macromolecules and is involved in photosynthesis and cell respiration What does nutrient limitation mean? When a nutrient is limited the primary productivity in the ecosystem will be limited as well- productivity depends entirely on the most limiting nutrient What is the difference between climate and weather? Weather: conditions at a specific time and place Climate: average conditions for a specific region What is the greenhouse effect? Natural layer of gases in the atmosphere that acts as an insulator to the planet Explain how biotic and abiotic factors are different. Give examples of each. Biotic factors are all factors that are living (ex: predator/prey relationships) Abiotic factors are the nonliving factors (ex: weather and nutrient availability) What is a niche? What does the competition exclusion principle state? An organisms role in its ecosystem including where it lives, the factors influencing the organism and how it uses those factors List and explain the 5 types of interaction organisms may have in a habitat. Competition: fighting for resources Predation: one animal hunts and kills another for food Mutualism: symbiosis where both organisms benefit from one another Commensalism: symbiosis where one organism benefits and the other is neither hurt nor helped Parasitism: symbiosis where one organism benefits and the other is harmed What is a biome? How are aquatic ecosystems classified? A group of ecosystems with the same climate and communities- aquatic ecosystems are classified by abiotic factors such as light availability and currents