Download (2010) A low-pathogenic variant of Infectious Salmon Anemia Virus

OIE Reference Laboratory Reports
Activities in 2010
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Infectious salmon anaemia
Address of laboratory:
National Veterinary Institute
P.O.Box 750 Sentrum
N-0160 Oslo, Norway
Tel.:
(+47-23) 21.60.00
Fax:
(+47-23) 21.60.01
e-mail address:
[email protected]
website:
www.vetinst.no
Name of Head of Laboratory
(Responsible Official):
Name of OIE Reference Expert:
Harald Gjein
Birgit Helene Dannevig
Name of writer of this report
(if different from above):
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
1
Infectious salmon anaemia
Part I: Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
Autopsy
Macroscopic lesions
Group
50*
Histopathology
Microscopic lesions
Group
200
Immunohistochmistry
Antigen
Group
<10
Immunofluorescence -Tissue
imprints
Antigen
Group
120
Cell culture/IFAT
Virus isolation/identification
Group
70
Real-time RT-PCR
Virus RNA
Group
1420
Sequencing
Gene (segment 5 and 6)
Genotype
60
*Autopsy is mainly carried out by local officers from the Norwegian Food Safety Authority
2.
Production and distribution of diagnostic reagents
Type of reagent
Amount supplied nationally
(including for own use)
Cell cultures (SHK-1, ASK)
2 flasks of SHK-1
ISAV reference strain
83 ml
Monoclonal anti ISAV HE
antibody
Amount supplied to other
countries
6 ampoules of 2 ml each
1 ampoules
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
Proficiency tests
Organized proficiency tests (“ring trials”) which included ISA-virus for the following diagnostic laboratories:
- Institute for Experimental Pathology, University of Iceland, Department of Fish Disaeses, Keldur
v/Vesturlandsveg 112-Reykjavik, Iceland
- Havbruksinstituttet, Thormøhlensgate 55, N-5008 Bergen, Norway (National laboratory performing diagnostic
tests for the Norwegian Food Safety Authority
Validation of methods
Validation of two real-time RT-PCR methods for detection of ISAV has been performed:
- the assay for detection of ISAV segment 7 and 8 respectively (Snow et al. 2006 Dev. Biol., 126, 133-145.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Infectious salmon anaemia
- the method for detection of segment 7 (Plarre et al. 2005 Dis. Aquat. Org., 66, 71-79) which has been adapted to
one-step RT-PCR conditions.
The validation was conducted using kidney-, heart- and gill tissues from both ISAV infected and healthy Atlantic
salmon. ISAV strains represented each of the established European subtypes including the putative low-pathogenic
strain ISAV HPR0. To identify cross-reactivity of the oligos with genomic sequences present in some of the
potential hosts of the ISAV, each assay was tested on tissue samples from tentative pathogen-free rainbow trout,
herring, turbot and char.
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
See also paragraph 2
ISAV (cell culture supernatant of reference strain, 1 vial of 1 ml) has been supplied to laboratories in the following
countries:
- Denmark
- Estonia
- Lithuania
- 3 National laboratories (one vaccine company and 2 laboratories performing diagnostic tests for the Norwegian
Food Safety Authority.
SHK-1 cell line
- National laboratory (vaccine company)
Antibodies
- Monoclonal anti ISAV HE antibody
5.
Research and development of new procedures for diagnosis and control
Tracing viral disease dissemination in aquaculture: an interdisciplinary approach between molecular virology and
dispersal modelling (Part II) (Project management: NVI. Financed by the Research Council of Norway, RCN). In
collaboration with a parallell project in Chile.
Infectious salmon anaemia. Pathogenesis and characterisation of the different manifestations of ISA as seen by
histopathological examinations and IHC of diseased fish from ISA field outbreaks (Internal project at NVI).
Vaccine-related projects
Virulence and Host-Pathogen Interactions. Part the Atlantic Innovation Fund Canada programme: “Mitigation of
ISA by Vaccination and Genetic Selection” (Project management: Novartis Aqua Health, PEI, Canada)
Host-virus interactions in Atlantic salmon (Project management: NVI. Financed by RCN, Norway)
Platform for Viral Aquamedicine (Project management: NVI. Financed by RCN, Norway)
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
Epizootiological data are continously collected from recent outbreaks of ISA. All ISAV isolates are currently
sequenced and genotyped
7.
Provision of consultant expertise to OIE or to OIE Members
Second Global Conference of OIE Reference Laboratories and Collaborating Centres, OIE Headquarters, Paris,
France, 21–23 June 2010. Participant from ISA-reference laboratory: Brit Hjeltnes, Deputy Director, Fish and
Shellfish Health.
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
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Infectious salmon anaemia
8.
Provision of scientific and technical training to personnel from other OIE Members
Training course at the National Veterinary Institute 6 – 10 September 2010 including lessons and practical
demonstrations in the diagnostic of ISA and other fish diseases. For ISA, virology (cell culture and PCR) and
histopathology were the primary topics.
Personnel trained:
9.
2 from Chile; 2 from the Republic of Korea
Provision of diagnostic testing facilities to other OIE Members
None
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
International Symposium on Infectious Salmon Anaemia in Oslo, Norway, 13-15 September 2010.
Co-organisers: Norwegian Food Safety Authority, OIE
Number of participants: 130. The participants represented research institutes, universities and national authorities
from all major contries producing Atlantic Salmon.
Output:
The objectives for the symposium were presentations and discussion of scientific results on the
infectious salmon anaemia (ISA)-virus and the disease, biosecurity and control strategies. A one-day workshop
was allocated to risk management with special emphasis on the management of farms of which low pathogenic
ISAV (ISAHPR0) have been detected. The abstracts were published in an abstract book distributed at the
symposium.
11. Participation in international scientific collaborative studies
None relevant studies
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
International conferences
Hauge, LH, Ramsell, J., & Mjaaland, S.(2010): Virus-host interactions during infectious salmon anaemia virus
infection.” Poster presentation First Symposium of the European Organisation of Fish Immunology, May 23rd 27th, 2010, Viterbo, Italy
Oral presentations at the International Symposium on Infectious Salmon Anaemia, 13-15 September, 2010, Oslo,
Norway :
Aldrin, M., Lyngstad, T.M., Kristoffersen, A.,B Storvik, B., Jansen, P., Borgan. Ø. Modeling the spread of
infectious salmon anaemia (ISA) in marine salmon farms in Norway based on sea distances between farms and
genetic distances between the ISA viruses from different outbreaks
Aamelfot, M., Dale, O.B., Weli, S., Koppang, E.O., Falk, K. Infection of atlantic salmon (salmo salar) endothelial
cells by Infectious salmon anaemia virus
Christiansen, D.H., Østergaard, P.S., Snow, M., Falk, K. Infectious Salmon Anemia Virus (ISAV) of the HPR0
subtype is highly prevalent and causes a non-clinical transient infection in farmed Atlantic salmon (Salmo salar L)
in the Faroe Islands
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Infectious salmon anaemia
Falk, K., Dale, O.B. Experimental evidence indicating that the balance between receptor binding- and receptor
destroying activity is a major factor for Infectious salmon anaemia (ISA) pathogenesis.
Falk, K., Dale, O.B. Immunohistochemistry evaluated as a diagnostic method for Infectious salmon anaemia (ISA)
Falk, K., Dale, O.B. A retrospective study of ISA in farmed Atlantic salmon by IHC and RT-PCR using formalinfixed paraffin-embedded tissue samples
Hauge, LH, Ramsell, J., Hedfors, I., Grimholt,U., Mjaaland, S. Targeting of infectious salmon anemia virus
antigens to salmonid antigen presenting cells.
Hetland, D., Dale, O.B., Skjødt, K., Press, CMcL., Falk, K. Depletion of CD8 alpha cells from tissues of Atlantic
salmon during the early stages of infection with high or low virulent strains of infectious salmon anaemia virus
(ISAV)
Hjortaas, M., Trettenes, E., Markussen, T. Infectious salmon anaemia virus (ISAV) loads in different organs in
diseased and asymptomatically infected fish
Karlsen, K., Johansen, E.J., Lyngstad, T.M. Jansen, P.A. Infectious salmon anaemia (ISA) in Norwegian salmon
farming, a chronicle of events relating to a small scale epidemic
Lyngstad, T.M., Hjortaas, M.J., Kristoffersen, A.B., Markussen, M., Karlsen, E., Jonassen, C.M., Jansen, P.A.
Using molecular epidemiology to trace the spread of infectious salmon anaemia virus (ISAV) in Norwegian
salmon farming
Ramsell, J., Hauge, H., Sobhkhez, M., Jørgensen, J., Mjaaland, S. Infectious salmon anemia: virus-host
interactions
Rimstad, E., Falk, K., Mjaaland, S, Kibenge, F. Characterization of infectious salmon anemia virus.
Weli, S., Dale, O.B, Aamelfot, M.,Koppang, E.O., Falk, K. ISAV infection of atlantic salmon gills
National meetings
Ramsell, J., Hauge, H., Sobhkhez, M., Jørgensen, J. & Mjaaland, S. (2010): Infeksiøs lakseanemivirus (ILAV):
virus-vert interaksjoner (ISAV: virus-host interactions). Oral presentation. Norwegian Research Council
Programme Conference (Havbruk), Trondheim 19.-21. April 2010.
Mjaaland, S. (2010).:”Grunnleggende kunnskap for vaksineutvikling” / ”Basic knowledge for vaccine
development”NRC/FHF
Strategic
meeting
Trondheim
1
June
2010.
Invited
lecture.
http://www.fhl.no/fou/strategisamling-fhf-fou-verdikjede-laks-article3573-25.html
Mjaaland, S. (2010): “Kunnskapsplatform for viral aquamedisin / Platform for Viral Aqua Medicine”. Invited lecture.
NRC meeting, Oslo 28th June, 2010

Scientific publications in peer-reviewed journals
Abayneh T, Toft N, Mikalsen AB, Brun E, Sandberg M. (2010) Evaluation of histopathology, real-time PCR and
virus isolation for diagnosis of infectious salmon anaemia in Norwegian salmon using latent class analysis. J. Fish
Dis. 33(6):529-32. (Epub 2010 Mar 8).
Aldrin M, Storvik B, Frigessi A, Viljugrein H, Jansen PA. (2010) A stochastic model for the assessment of the
transmission pathways of heart and skeleton muscle inflammation, pancreas disease and infectious salmon
anaemia in marine fish farms in Norway. Prev Vet Med. 93(1):51-61. Epub 2009 Oct 6.
Christiansen DH, Ostergaard PS, Snow M, Dale OB, Falk K. (2010) A low-pathogenic variant of Infectious
Salmon Anemia Virus (ISAV-HPR0) is highly prevalent and causes a non-clinical transient infection in farmed
Atlantic salmon (Salmo salar L.) in the Faroe Islands. J Gen Virol. Dec 9. [Epub ahead of print]
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
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Infectious salmon anaemia
Hetland DL, Jørgensen SM, Skjødt K, Dale OB, Falk K, Xu C, Mikalsen AB, Grimholt U, Gjøen T, Press CM.
(2010) In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in
infectious salmon anaemia virus (ISAV)-infected Atlantic salmon. Fish Shellfish Immunol. 28(1):30-39.
Müller A, Markussen T, Drabløs F, Gjøen T, Jørgensen TØ, Solem ST, Mjaaland S. (2010) Structural and
functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virus. Virus Res. 151(2):131-141.
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register? If yes, for which kits?
No
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
which kits?
No
_______________
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010