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THE EFFECT OF ZINC STATUS ON PROINFLAMMATORY RESPONSE IMPLICATIONS FOR AGE-RELATED CHRONIC INFLAMMATION Nicole Rinaldia, Carmen Wong, PhDb, Emily Ho, PhDb,c aDepartment of BioResource Research bSchool of Biological and Population Health Sciences cLinus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA BACKGROUND Zinc is an essential micronutrient Important role in immune system health Zinc deficiency causes chronic lowgrade inflammation Chronic inflammation promotes development of degenerative disease Heart disease, diabetes, autoimmune disease, cancer BACKGROUND Aging is strongly associated with chronic inflammation Zinc status declines with age 12% of total US population is zinc deficient, however 40% of elderly Americans are zinc deficient Due to both decreased absorption of zinc and consumption of zinc in the elderly RESEARCH QUESTIONS What level of zinc deficiency causes immune dysregulation? Can zinc supplementation improve immune function? HYPOTHESIS Zinc deficiency will increase the intensity of the proinflammatory response compared to that elicited by zinc adequate conditions Zinc supplementation will decrease the intensity of the proinflammatory response compared to that elicited by zinc adequate conditions METHODS THP-1 human leukemic monocyte cell culture model Monocytes are a form of white blood cell involved in proinflammatory response Cells are cultured in media that is either zinc deficient (0 μM zinc), marginally zinc deficient (1 μM zinc), zinc adequate (4 μM zinc), or zinc supplemented (40 μM zinc) METHODS Day 25 ZA Day 25 ZD Monocytes undergo measurable processes during induction of proinflammatory response Increased ICAM1 expression, increased production of proinflammatory cytokines, increased generation of reactive oxygen species (ROS) RESULTS Expression of Cell Activation Marker ICAM1 Production of Proinflammatory Cytokines IL1β & IL6 ZA= zinc adequate MZD= marginal zinc deficient Production of Reactive Oxygen Species (ROS) ZD= zinc deficient ZS= zinc supplemented CONCLUSIONS Effect of zinc supplementation on intensity of proinflammatory response is unclear at this time Zinc deficiency and marginal zinc deficiency increase intensity of proinflammatory response Increased production of cell activation markers, proinflammatory cytokines, and ROS Zinc deficiency and marginal zinc deficiency may contribute to chronic inflammation experienced by the elderly ACKNOWLEDGEMENTS LIFE Scholars Summer Research Program Dr. Emily Ho – Mentor Endowed Director, Professor, Principal Investigator OSU College of Public Health and Human Sciences Dr. Carmen Wong – Mentor Research Associate, Nutrition & Exercise Sciences OSU College of Public Health and Human Sciences Wanda Crannell – Advisor Advisor / Instructor OSU College of Agricultural Sciences RESULTS