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Contents Chapter 1. General introduction and objectives Chapter 2. Testing endocrine disruption in biota samples: a method to remove interfering lipids and natural hormones Chapter 3. Blood plasma sample preparation method for the assessment of thyroid hormonedisrupting potency in effect-directed analysis Chapter 4. Transthyretin-binding activity of contaminants in blood from polar bear (Ursus maritimus) cubs Chapter 5. Effect-directed analysis to explore the polar bear exposome: the identification of thyroid hormone disrupting compounds in plasma Chapter 6. Effect-directed analysis (EDA) of genotoxic and thyroid hormone-disrupting compounds in abiotic and biotic samples from aquatic ecosystems Chapter 7. General discussion, concluding remarks and outlook Summary Samenvatting Curriculum Vitae List of publications List of oral presentations Acknowledgement