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Transcript
YOUR GLOBAL PARTNER IN DIAGNOSTICS SERION Complement Fixation Test (CFT) The Complement Fixation Test (CFT) is a classical serological method for the detection of antibodies directed against pathogens of infectious diseases and has proved itself as a standard diagnostic method in many medical laboratories. With SERION CFT reagents, Institut Virion\Serion GmbH offers a great variety of antigens that are important in infectious serology of bacteria, viruses and parasites. The complement fixation test performance is a micromethod according to the KOLMER technique and to DIN 58 969 as well as to the WHO guidelines. Complement Complement is a system of functionally connected serum proteins, which are important components of the humoral immune system and are responsible for several biological functions involved in the process of infections. In the course of complement activation several proteins react in a distinct order similar to the cascade in blood coagulation. In the classical activation the complement cascade is initiating by complexes of antigens and IgG1, IgG2, IgG3 und IgM antibodies, whereby the latter are the most efficient. In the so called alternative activation pathway parts of microorganisms may activate complement without the participation of antibodies. The SERION Complement Fixation Tests make use of the classical complement activation pathway. serion multianalytTM YOUR GLOBAL PARTNER IN DIAGNOSTICS Order Information CFT - Reagents Pathogen Adenovirus Brucella Campylobacter fetus / ssp. Campylobacter jejuni Chlamydia Coxiella burnetii (Phase I) Coxiella burnetii (Phase II) Coxsackievirus A9 Coxsackievirus B1 Coxsackievirus B2 Coxsackievirus B3 Coxsackievirus B4 Coxsackievirus B5 Coxsackievirus B6 Coxsackievirus Pool (A9, B1 - B6) Cytomegalovirus (CMV) Echovirus Pool (4, 6, 9, 14, 24, 30) Epstein-Barr Virus (EBV) TBE Virus Herpes Simplex Virus (HSV) 1 / 2 Influenza A Virus Influenza B Virus Influenza A/B Virus Pool Legionella pneumophila Leptospira biflexa Leptospira canicola Leptospira grippotyphosa Leptospira icterohaemorrhagiae Leptospira pomona Leptospira sejroe Antigen 1,0 ml Control Antigen 1,0 ml Pos. Control Serum 0,1 ml Neg. Control Serum 0,1 ml 1121 1297 1207 1206 1122 1227 1123 9060 1172 1173 1174 1175 1176 1177 1178 1130 1180 1132 1192 1154 1112 1113 1114 1224 9120 9090 9070 9080 9100 9110 2121 2122 2227 2123 9260 2179 2179 2179 2179 2179 2179 2178 2130 2180 2132 2192 2154 2112 2113 2114 - 3121 3297 3207 3206 3122 3227 3123 9061 3172 3173 3174 3175 3176 3177 3178 3130 3180 3132 3192 3154 3112 3113 3114 3224 3120 9071 9071 9071 9071 9071 4121 4297 4207 4206 4122 4227 4123 9062 4172 4173 4174 4175 4176 4177 4178 4130 4180 4132 4192 4154 4112 4113 4114 4224 9072 9072 9072 9072 9072 9072 Pathogen 1,0 ml Control Antigen 1,0 ml Pos. Control Serum 0,1 ml Neg. Control Serum 0,1 ml 1234 1190 1125 1111 1253 1116 1117 1118 1115 1126 1127 1124 1193 1331 1191 1203 1209 1201 2190 2125 2116 2117 2118 2115 2126 2127 2124 2193 2331 2191 - 3234 3190 3125 3111 3253 3116 3117 3118 3115 3126 3127 3124 3193 3331 3191 3203 3209 3201 4234 4190 4125 4111 4253 4116 4117 4118 4115 4126 4127 4124 4193 4331 4191 4203 4209 4201 Article Volume Article Nr. Complement Complement 5 x 1 ml Haemolytic amboceptor CFT buffer 1 ml 5 x 1 ml 2 ml 2l 9001 9001.5 9002 9009 Listeria monocytogenes Measles Virus Mumps / Parotitis Virus Mycoplasma pneumoniae Neisseria gonorrhoeae Parainfluenza Virus 1 Parainfluenza Virus 2 Parainfluenza Virus 3 Parainfluenza Virus Pool (1, 2, 3) Picornavirus Poliovirus Resp. Syncytial Virus (RSV) Rotavirus Toxoplasma gondii Varicella-Zoster Virus (VZV) Yersinia enterocolitica O3 Yersinia enterocolitica O9 Yersinia pseudotuberculosis Antigen Order Information Supplementary CFT - Reagents We recommend to source erythrocyte suspensions, which are compatible with our complement fixation tests, from Labor Dr. Merk & Kollegen GmbH (Ochsenhausen, Germany; www.labormerk.com). Test Principle Diagnostics The first step in the SERION Complement Fixation Tests is the thermal inactivation of patient’s serum samples to inactivate endogenous complement which may disturb the test calibration. Antigen is then added, leading to the formation of specific antibody-antigen complexes. Subsequent addition of complement will, in the presence of antibody-antigen complexes, lead to activation of the complement. An indicator system consisting of antibody coated erythrocytes (hemolytic system) is finally added to the system after a suitable time lapse for the complement fixation to complete. Lysis of the indicator system is dependent upon the presence or absence of immune complexes formed between the sample and specific antigen used. No lysis indicates the presence of immune complexes while lysis indicates non. Mild centrifugation of the system sediments any surviving erythrocytes, allowing the test to be evaluated visually. A button of red cells indicates a positive whereas a reddish clear solution is negative. Complement fixation tests can be used as screening tests for acute infections. The fact that one IgM molecule is capable of activating one C1 molecule of the complement complex whereas 168 IgG molecules are necessary to achieve the same effect, emphasises the significance of the test for the recognition of early infections. Positive CFT titers are often an indication for the presence of IgM antibodies or very high IgG antibody titers and are therefore an expression for an acute or recent infection, respectively. The CFT is especially helpful for acute respiratory infections: The, in general, strong IgG booster reaction is very well represented whereas residual titers of past infections are blanked out. The distinction between IgG and IgM antibodies is not possible. For determination of immune status the sensitivity of the method is, unfortunately, not sufficient. For more differentiated information about the immune status we recommend the use of the corresponding SERION ELISA classic for the separate detection of different immunoglobulin classes. Advantages of SERION CFT Products • • • • • • • • • Large product portfolio for the indirect detection of various pathogens. High specificities by use of carefully selected antigens and control antigens. Early detection of acute primary infections and therapy sucesses. Possibility to use SERION CFT as an economic, cost-effective screening assay. Consistent complement and amboceptor concentration for all SERION CFT antigens. Combination of different CFTs on one plate possible. Normed CFT, therefore omission of pre-experiments in routine laboratories. Long stabilities of antigens, control antigens, positive and negative control sera. Use in human and veterinary diagnostics. Institut Virion\Serion GmbH Friedrich-Bergius-Ring 19 97076 Würzburg Germany Tel.: +49 (0)931 3045-222 Fax: +49 (0)931 3045-100 [email protected] www.virion-serion.com Institut Virion\Serion GmbH, V4.14/12