Download SERION Complement Fixation Test (CFT)

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SERION
Complement Fixation Test (CFT)
The Complement Fixation Test (CFT) is a classical serological method for the detection of antibodies directed
against pathogens of infectious diseases and has proved itself as a standard diagnostic method in many
medical laboratories. With SERION CFT reagents, Institut Virion\Serion GmbH offers a great variety of
antigens that are important in infectious serology of bacteria, viruses and parasites. The complement fixation
test performance is a micromethod according to the KOLMER technique and to DIN 58 969 as well as to the
WHO guidelines.
Complement
Complement is a system of functionally connected serum proteins, which are important components of the humoral immune
system and are responsible for several biological functions involved in the process of infections. In the course of complement
activation several proteins react in a distinct order similar to
the cascade in blood coagulation. In the classical activation the
complement cascade is initiating by complexes of antigens and
IgG1, IgG2, IgG3 und IgM antibodies, whereby the latter are the
most efficient. In the so called alternative activation pathway
parts of microorganisms may activate complement without the
participation of antibodies. The SERION Complement Fixation
Tests make use of the classical complement activation pathway.
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Order Information CFT - Reagents
Pathogen
Adenovirus
Brucella
Campylobacter fetus / ssp.
Campylobacter jejuni
Chlamydia
Coxiella burnetii (Phase I)
Coxiella burnetii (Phase II)
Coxsackievirus A9
Coxsackievirus B1
Coxsackievirus B2
Coxsackievirus B3
Coxsackievirus B4
Coxsackievirus B5
Coxsackievirus B6
Coxsackievirus Pool (A9, B1 - B6)
Cytomegalovirus (CMV)
Echovirus Pool (4, 6, 9, 14, 24, 30)
Epstein-Barr Virus (EBV)
TBE Virus
Herpes Simplex Virus (HSV) 1 / 2
Influenza A Virus
Influenza B Virus
Influenza A/B Virus Pool
Legionella pneumophila
Leptospira biflexa
Leptospira canicola
Leptospira grippotyphosa
Leptospira icterohaemorrhagiae
Leptospira pomona
Leptospira sejroe
Antigen
1,0 ml
Control
Antigen
1,0 ml
Pos. Control
Serum
0,1 ml
Neg. Control
Serum
0,1 ml
1121
1297
1207
1206
1122
1227
1123
9060
1172
1173
1174
1175
1176
1177
1178
1130
1180
1132
1192
1154
1112
1113
1114
1224
9120
9090
9070
9080
9100
9110
2121
2122
2227
2123
9260
2179
2179
2179
2179
2179
2179
2178
2130
2180
2132
2192
2154
2112
2113
2114
-
3121
3297
3207
3206
3122
3227
3123
9061
3172
3173
3174
3175
3176
3177
3178
3130
3180
3132
3192
3154
3112
3113
3114
3224
3120
9071
9071
9071
9071
9071
4121
4297
4207
4206
4122
4227
4123
9062
4172
4173
4174
4175
4176
4177
4178
4130
4180
4132
4192
4154
4112
4113
4114
4224
9072
9072
9072
9072
9072
9072
Pathogen
1,0 ml
Control
Antigen
1,0 ml
Pos. Control
Serum
0,1 ml
Neg. Control
Serum
0,1 ml
1234
1190
1125
1111
1253
1116
1117
1118
1115
1126
1127
1124
1193
1331
1191
1203
1209
1201
2190
2125
2116
2117
2118
2115
2126
2127
2124
2193
2331
2191
-
3234
3190
3125
3111
3253
3116
3117
3118
3115
3126
3127
3124
3193
3331
3191
3203
3209
3201
4234
4190
4125
4111
4253
4116
4117
4118
4115
4126
4127
4124
4193
4331
4191
4203
4209
4201
Article
Volume
Article Nr.
Complement
Complement 5 x 1 ml
Haemolytic amboceptor
CFT buffer
1 ml
5 x 1 ml
2 ml
2l
9001
9001.5
9002
9009
Listeria monocytogenes
Measles Virus
Mumps / Parotitis Virus
Mycoplasma pneumoniae
Neisseria gonorrhoeae
Parainfluenza Virus 1
Parainfluenza Virus 2
Parainfluenza Virus 3
Parainfluenza Virus Pool (1, 2, 3)
Picornavirus
Poliovirus
Resp. Syncytial Virus (RSV)
Rotavirus
Toxoplasma gondii
Varicella-Zoster Virus (VZV)
Yersinia enterocolitica O3
Yersinia enterocolitica O9
Yersinia pseudotuberculosis
Antigen
Order Information Supplementary CFT - Reagents
We recommend to source erythrocyte suspensions, which are compatible with our complement fixation tests, from
Labor Dr. Merk & Kollegen GmbH (Ochsenhausen, Germany; www.labormerk.com).
Test Principle
Diagnostics
The first step in the SERION Complement Fixation Tests
is the thermal inactivation of patient’s serum samples to
inactivate endogenous complement which may disturb
the test calibration. Antigen is then added, leading to
the formation of specific antibody-antigen complexes.
Subsequent addition of complement will, in the presence
of antibody-antigen complexes, lead to activation of the
complement. An indicator system consisting of antibody
coated erythrocytes (hemolytic system) is finally added
to the system after a suitable time lapse for the complement fixation to complete. Lysis of the indicator system
is dependent upon the presence or absence of immune
complexes formed between the sample and specific antigen used. No lysis indicates the presence of immune
complexes while lysis indicates non. Mild centrifugation of the system sediments any surviving erythrocytes,
allowing the test to be evaluated visually. A button of red
cells indicates a positive whereas a reddish clear solution
is negative.
Complement fixation tests can be used as screening tests for
acute infections. The fact that one IgM molecule is capable of
activating one C1 molecule of the complement complex whereas
168 IgG molecules are necessary to achieve the same effect,
emphasises the significance of the test for the recognition of
early infections. Positive CFT titers are often an indication for
the presence of IgM antibodies or very high IgG antibody titers
and are therefore an expression for an acute or recent infection,
respectively. The CFT is especially helpful for acute respiratory
infections: The, in general, strong IgG booster reaction is very
well represented whereas residual titers of past infections are
blanked out. The distinction between IgG and IgM antibodies is
not possible. For determination of immune status the sensitivity
of the method is, unfortunately, not sufficient. For more differentiated information about the immune status we recommend the
use of the corresponding SERION ELISA classic for the separate
detection of different immunoglobulin classes.
Advantages of SERION CFT Products
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Large product portfolio for the indirect detection of various pathogens.
High specificities by use of carefully selected antigens and control antigens.
Early detection of acute primary infections and therapy sucesses.
Possibility to use SERION CFT as an economic, cost-effective screening assay.
Consistent complement and amboceptor concentration for all SERION CFT antigens.
Combination of different CFTs on one plate possible.
Normed CFT, therefore omission of pre-experiments in routine laboratories.
Long stabilities of antigens, control antigens, positive and negative control sera.
Use in human and veterinary diagnostics.
Institut Virion\Serion GmbH
Friedrich-Bergius-Ring 19
97076 Würzburg
Germany
Tel.: +49 (0)931 3045-222
Fax: +49 (0)931 3045-100
[email protected]
www.virion-serion.com
Institut Virion\Serion GmbH, V4.14/12