Download NPH_3820_sm_FigS1-S7-TableS1

6.6
6.5
6.4
Bacteria
(log cfu / cm2 of
tissue)
6.3
6.2
6.1
6
5.9
5.8
5.7
5.6
WT
35S::At4g29150
Supporting Information Fig. S1 Over-expression of
At4g29150 does not enhance resistance against
PstDC3000. Wild-type and 35S::At4g29150 plants were
inoculated with PstDC3000 and bacterial growth scored at 5
days post challenge. Data points are the average of three
technical replicates (±SD). This experiment was repeated
with similar results.
WT
ads1-D
Supporting Information Fig. S2 Callose deposition in the
ads1-D line relative to wild-type plants at 6 hours post
inoculation of PspNPS3121. Images were generated at 20 X
with a Nikon Eclipse E600 epifluorescence microscope using
a UV filter.
WT
ads1-D
Supporting Information Fig. S3 Callose deposition in the
ads1-D line relative to wild-type plants at 48 hours post
inoculation of Bgt. Images were generated at 20 X with a
Nikon Eclipse E600 epifluorescence microscope using a UV
filter.
7
Disease index
6
5
4
3
2
1
0
WT
ads1-D 35S::ADS1
Supporting Information Fig. S4 Challenge of the given
Arabidopsis genotypes with B. cinerea. The PJH2 isolate of
B. cinerea (Nurmberg et al., 2007) was grown on oat meal
and 1⁄2 potato dextrose agar media, respectively. Fungal
spore density
was adjusted to 5 x 105 spores per ml in 1⁄2 potato dextrose
broth. This B. cinerea spore suspension was sprayed onto
4-week-old Arabidopsis plants that were maintained at
100% humidity for 4 days. Data points are the average of
three replicate samples (±SD). Student’s t test confirmed
there were no significant differences at p = 0.05 between
pathogen growth in ads1 lines relative to WT plants.
7.5
Bacteria
(log cfu / cm2 of tissue)
6.5
5.5
4.5
3.5
2.5
1.5
WT
rADS1-2
rADS1-3
Supporting Information Fig. S5 Depletion of ADS1 transcript
accumulation promotes basal resistance. Growth of
PstDC3000 in wild-type and two additional rADS1 lines at 0
(hatched bar), 3 (white bar) and 5 (black bar) dpi. Data points
are the average of three technical replicates (±SD). Student’s
t test confirmed significant differences at p = 0.05 between
bacterial growth in the rADS1 lines relative to WT plants. This
experiment was repeated with similar results.
ads1-D
35S::ADS1
WT
Supporting Information Fig. S6 ROI accumulation in ads1-D,
35S::ADS1 and wild-type plants in the absence of pathogen
challenge. The levels of ROIs in the indicated genotypes were
determined by DAB staining. Two representative DAB stained
leaves for each plant line are shown.
ads1-D
WT
ads1-D
rbohD
ads1-D
rbohF
ads1-D
rbohD rbohF
Supporting Information Fig. S7 ROI accumulation in
ads1-D double and triple mutants. The levels of ROIs in
ads1-D, wild-type, ads1-D rbohD, ads1-D rbohF and ads1-D
rbohD rbohF were determined by DAB staining. Three
representative DAB stained leaves for each plant genotype
are shown.
Supporting Information Table S1 Change in ADS1
transcript levels during attempted pathogen infection or in
response to defence-related cues or abiotic stresses. All
data from Genevestigator at http://www.arabidopsis.org/.
Treatment
Average FoldInduction Relative to
Untreated Control
Defence signalling molecules
Ethylene
Methyl-jasmonate (0.5 h)
Methyl-jasmonate (2 h)
Methyl-jasmonate (6 h)
Salicylic acid
3.1
1.6
2.7
1.6
-1.4
Biotic stress
PstDC3000 (avrB)
PstDC3000 (avrRPS4)
B. graminis
-1.4
-1.3
-2.0
Abiotic stress
Cold shock
Heat shock (roots)
-1.63
-1.58