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What’s fueling genome evolution? (Understanding shared genomic resources inprokaryotes) • PI: Janet Siefert, Rice University; CO-PI’s: Yuiry Fofanov, University of Houston; George Fox, University of Houston; Peter Gogarten, U. Conn; Dr. Maia Larios-Sanz, Rice University; Dr. Jason Raymond, Lawrence Livermore; Dr. Carlos Solis, Rice University; Dr. Valeria Souza, UNAM; Mike Travisano, University of Houston. What’s fueling genome evolution? (Understanding shared genomic resources in prokaryotes) Shared Genome Resource Selection Functional Integration We propose to investigate horizontal gene flow in bacteria by integrating genomic analysis, microbial ecology, and experimental selection systems. Established and novel approaches performed by a diversely talented team of investigators will integrate results in a check-and-balance system. Using three phylogenetically distinct lineages of bacteria, we will investigate and quantify the shared genome resource available for transfer and the functional integration of that shared resource. The proposed research and interdisciplinary training will initiate a comprehensive framework in which to view prokaryotic genome evolution. Genome analysis Framework of HGT Estimates Microbial Ecology Experimental selection Three organisms Bacillus – gram positive, low G+C, ubiquitous soil/water Cyanobacteria – oxygen producers E. coli – gram negative, protobacter, mammalian gut Agenda 1. Introduction to the overall project (Janet - 10 minutes) 2. Welcome Jason, quick bio (Jason - 5 minutes) 3. Introduction of Cuatro Cienegas (Valeria, Janet - 20 minutes, see attached powerpoint and http://www.laventa.it/photogallery/4c-pozze/index.htm) 4. Return to project scheme and flesh out individual contributions, old and new methodology ideas, discuss integration schemes, writing assignments.(1 hour) 5. Grant issues: advisory committe, deadlines, efforts. (20 minutes) Genome Analysis Experimental Systems S-Plots – Yuiry Nested timing-Direction of HGT Unit of Selection E.coli Cyano Bacillus Pseudomonas reporter strains Ecology Bacillus – Homologous recombination E.coli Virus complement Grant Items • Advirsory Board • • • • • • • • Ed DeLong Ford Doolittle Grauer Jonathan Eisen David Lynch Jill Banfield Dave Ward Forrest Rohwer (Virus) • Deadlines • Writing Assignments