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Plant Propagation • One of the most rewarding and satisfying activities in horticulture is plant propagation. • Plant propagation is the heart of all work in the field. What is plant propagation? • Making new plants from old plants • There are two (2) types of propagation: sexual asexual (vegetative) Why propagate plants • To multiply numbers • To save wanted traits • To improve a species of plants • Biotechnology helps improve food production, quality of flowers, and resistance to disease in plants. • Gene manipulation may someday cure many of our most deadly diseases. Sexual Propagation • Involves the union of pollen with an egg in the ovary. • These male and female cells may be from the same plant (self-pollination) or from • Separate parent plants (cross-pollination) • Seeds are a way of rapidly increasing the number of a certain plant. • Many plants reproduced from seed will differ from the original plants. • This is because genes from each of the parent plants are represented in the new plant. Thus, a variety is created. Principles of Genetics • Gregor Mendel, an Austrian Monk, is recognized for discovering the basic principles of genetics. • Genetics is the study of how characteristics are passed from parents to offspring. • He used observations of pea plants to develop these principles: • In all organisms, there is a pair of genes in every cell that determines every trait in that individual organism. • Individuals receive one gene for each trait from each parent. • Genes are transmitted from parent to offspring as unchanging units. • In the making of reproductive cells, gene pairs separate; with only one gene for each trait is contained in each gamete. (sex cells: pollen or eggs) Each gene passed on by the parent plant is called an allele • A visible characteristic is called a phenotype • The combination genes from parent plants is the genotype • When different genes are present for a single trait, in most cases only one trait will be expressed (seen) in the offspring. • The seen trait is called a dominant trait The masked or unseen trait is a recessive trait • A dominant allele is written as an upper case letter symbol (T) • A recessive allele is written as a lowercase letter symbol (t) • In plants, the color red is a dominant trait, white flowers are a recessive trait. • If a plant has two (2) dominant genes for a trait, it is called pure dominant. (TT) • Dominant homozygous • A plant with two(2) recessive genes is pure recessive (tt) • recessive homozygous • A plant with two (2) different genes for a trait (Tt) • It is heterozygous. • Even though the individual has the recessive gene, the recessive trait is not seen. Only the dominant feature shows up Expected & Observed Results • Question: How can knowing the types of genes that each parent has be helpful? • Traits the offspring will have can be predicted • The color, size and shape of the petals can be pre-determined by understanding what traits will appear when two plants are cross-pollinated. • Biotechnology allows us to build or re-build plants to produce better quality flowers, fruit or growth habit by combining the best traits of different plants. Hybrid Seed • Hybrid seed are specially produced seed that germinate into plants which appear to be genetically identical. • If seed are taken from these hybrid plants, the resulting plants will have a variety of combinations of traits. If only the original traits are desired, do not use seed from hybrid plants. • Chose seed from named varieties only. Sowing Seed • Seed propagation of is used for many herbaceous annuals, and perennials. • The proper environmental and cultural conditions must be provided: 1) temperature 2) moisture 3) light 4) media 1)Place media in container (water gently) 2)Slowly shake seed onto media a. broadcast: randomly & evenly shake out over the media b. row plant: place seed in even rows* (*best method to avoid spread of fungus /diseases) 3) cover seed with a thin layer of media 4) water gently 5) place in area with higher humidity, and increase soil temperature to specified level for the seed growing. (bottom heat range 65’ – 70’) 6) label container with Named variety. date sown Asexual Propagation • Reproduction without sex. • Occurs naturally with most plants, • Has become very important in the Horticultural Industry. • Clones – plants genetically identical being produced asexually (uses part of a plant) • Pluses to clones: The new plant is genetically identical The new plant is quicker to get to mature size. Disadvantages of Using Seed to Propagate New Plants • Seedlings may take too long to mature • Seed from some plants may be slow to germinate (Adds to Costs) • Varying genetics transfer to off spring with each generation. • Seed production is mainly used when growing bedding plants, (annuals,perennials, and vegetables) • Asexual Propagation Provides: • 1. uniformity of large numbers of plants • 2. to sustain wanted or desired traits (characteristics) • 3. rejuvenate sick or scraggly plants Methods of Propagating Plants Asexually • • • • • • • • • Stem Cuttings Cane Cuttings Leaf Cuttings Leaf-bud Cuttings Layering Air Layering Division and Separation Tissue Culture Grafting Grafting • The method of grafting is connecting two plants or plant parts together so they unite and continue to grow as one plant. • Grafting is possible only when the two plants are related at least at the family level. • A graft consists of two parts: • Scion: • A short piece of stem with 2 or more buds, becomes the top of the new plant • Understock: (rootstock) • The lower part of the graft. Becomes the root system of the new plant • Reason(s) for grafting • Propagate plants difficult to propagate by other means • Used most often in orchards, shade trees, and roses • Creates dwarf plants • Creates plants with more hardy characteristics Types of Grafts • Whip and tongue graft• Used of wood smaller than an inch • Allows vascular tissue to match up Types of Grafts • Cleft graft• Used when the understock is larger than the scion. • Vascular tissue must match up so scions are inserted at the edges of the understock Types of Grafting • Slip Graft (Side Graft)• Quickest graft, although hard to keep together (lined up) Types of Grafts • Saddle graft – scion is cut so it sits over the understock a very tight fit is achieved Budding/Bud Grafting • Similar to Grafting except the scion is a single bud with a small section of bark. • Practiced in early spring just before plants break dormancy. Types of Budding • T- Budding • buds removed from a plant with desirable traits then attached to an understock to form new plant • The rootstock is cut in a “T” shape, the bud is slipped into the cut and tied off with a band. It is then covered with beeswax Types of Budding • Patch Budding- desired bud is cut out like a stamp patch. The same shape patch is cut out of the understock. • The bud is placed into the patch on the understock then tied off and covered with beeswax • For both types of budding, the top of the rootstock is cut just above the new bud once it opens to become the new plant's top • Grafting is important to the horticulture industry because it has enabled growers to create new plants while using the best traits of others. • The wine industry of Europe was saved by the inferior rootstock of the 'Concord' Grape.