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Lab Review 7-12 When does the fun begin? Lab 7 – Genetics!!! If we were to cross flies: What would the parent generation consist of ? Homozygous Dominant + Homozygous Recessive Red eyes + Sepia eyes What would the F1 generation look like? ALL Heterozygotes ALL Red eyes In F2, what would the ratio be ? Phenotypes ? 3:1 (Red : Sepia) Genotypes ? 1 : 2 : 1 (HD : Het : HR) Chi-Squared Analysis Tells us whether the actual results are really different than the expected results If the chi-squared value > critical value, then the results are considered DIFFERENT than the expected values DIFFERENT = LINKED genes Typically sex-linked or X-linked Lab 8 – Hardy-Weinberg Remember the Teddy Bears?? Conditions for HW Equilibrium 1. A large breeding population 2. Random mating 3. No change in allelic frequency due to mutation 4. No immigration or emigration 5. No natural selection p = the frequency of the dominant allele q = the frequency of the recessive allele For a population in genetic equilibrium: p + q = 1.0 (p + q)2 = 1 p2 + 2pq + q2 = 1 The three terms of this binomial expansion indicate the frequencies of the three genotypes: p2 = frequency of AA (homozygous dominant) 2pq = frequency of Aa (heterozygous) q2 = frequency of aa (homozygous recessive) Complete Lab-Quiz http://www.phschool.com/science/biology_place/labbe nch/lab8/quiz.html Lab 9 - Transpiration Remember this Lab? Remember Transpiration?? Hydrogen Bonding Cohesion Adhesion Water Potential Water: HIGH Potential LOW Potential Highest Water Potential = 0 http://www.phschool.com/science/biology_place/labbe nch/lab9/test.html Stomata Stoma – hole that allows CO2 in or O2 out Guard cells – open or close stoma (plural = stomata) Q: How are stomata related to transpiration? A: Source of the transpirational pull Factors affecting Transpiration Light (increases / decreases) transpiration ? INCREASES – stomata open transpiration Humid environment (inc. /dec.) transpiration ? DECREASES – more water = higher water potential Windy (increase / decrease) transpiration ? INCREASE – less water = lower water potential Hot vs warm: which will increase transpiration ? HOT – increase temp = more evaporation Lab 10 – Cardiovascularity? Blood Pressure Systolic – BP when heart (ventricles) has contracted Diastolic – BP when heart (ventricles) is relaxed Sphygmomanometer – measures blood pressure http://www.phschool.com/science/biology_place/labbe nch/lab10/quiz1.html http://www.phschool.com/science/biology_place/labbe nch/lab10/quiz2.html?radio1=d&radio2=b&radio3=b&ra dio4=b&x=53&y=3 Lab 11 – Animal Behavior Isopods Fruit fly mating behavior Pillbug Behavior Moar Quiz!! http://www.phschool.com/science/biology_place/labbe nch/lab11/quiz.html Lab 12 – Dissolved Oxygen A dude left his fish tank on top of his radiator He saw plenty of bubbles, so he thought the situation was good for his fish Why did his fish perish? Factors that affect dissolved oxygen in water Temperature: As water becomes warmer, its ability to hold oxygen decreases. Photosynthetic activity: In bright light, aquatic plants are able to produce more oxygen. Decomposition activity: As organic material decays, microbial processes consume oxygen. Mixing and turbulence: Wave action, waterfalls, and rapids all aerate water and increase the oxygen concentration. Salinity: As water becomes more salty, its ability to hold oxygen decreases. Productivity Rate of photosynthetic activity Rate of production of organic compounds in an ecosystem Gross Productivity – Photosynthetic production of organic compounds in an ecosystem Net Productivity – organic material that remain after some has been used for cellular respiration Productivity Equation NET Productivity = GROSS Productivity – Respiration What is the Photosynthesis Equation ? CO2 + H2O + Energy Sugar + O2 How do we measure productivity? Oxygen Gas concentration When light, O2 is PRODUCED by PHOTOSYNTHESIS O2 is CONSUMED BY RESPIRATION When dark, O2 is CONSUMED by RESPIRATION ONLY Calculating Productivity Light D.O. – Dissolved O2 after 24 hours with exposure to light Photosynthesis + Respiration Dark D.O. – after 24 hours with no light Respiration ONLY Initial D.O. – before the experiment begins Baseline value of D.O. Photosynthesis Final Quiz http://www.phschool.com/science/biology_place/labbe nch/lab12/quiz.html