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Biochemistry The Atom Smallest unit of an ____________  Three particles  – Protons (___ charge) – Neutrons (__ charge) – Electrons (__ charge)  _________ contains protons and neutrons Elements  Pure substance consisting of only one type of ___________  Listed in the _______________  Atoms of each element differ by the # of ___________ Isotopes Atoms of an element with different number of __________  Atomic # is the _____; mass # is _________  Most have same chemical properties  Radioactive Isotopes  Uses of radioactive isotopes – Dating of rocks and fossils – Treating cancer – Killing bacteria on food – Trace movements of substances within the body Chemical Compounds  Substance of two or more ________ in definite proportions  __________ chemical properties than the original elements  Compound composition given in chemical formula – Examples: ______________ Chemical Reactions Breaking and forming ________________  _______- original elements or compounds  _______- ending elements or compounds  Monomer vs Polymer  ___________- building block basic repeating  _________- two monomers connected together  _________- many monomers connected together Dehydration Synthesis Building larger molecules from smaller ones (requires energy)  Monomer → Polymer  Hydrolysis Break down large molecules into smaller ones (releases energy)  Polymer → Monomer  Carbon Compounds Condensation/Synthesis Polymer Monomer Hydrolysis What is So Special About Carbon?  Carbon can bond with ______ other atoms  Creates a great variety of different compounds  Can for complex molecules including __________________________ Carbohydrates Made of C, H, and O in a _________ ratio  Monomers  – Monosaccharide – Examples: _______________________  Dimers – Disaccharides – Examples: _______________________  Polymers – Polysaccharides – Examples: _________________________ Carbohydrates (cont) Carbohydrates (cont) Carbohydrates (cont) Carbohydrates (cont) CHITIN Cellulose Uses for Carbohydrates  Provide  Used ________ for body functions to build nucleic acids  Other ____________ functions Tests for Carbohydrates  Benedict’s  Iodine Test for ________ sugars Test for __________ Sugars Lipids Made of C, H, and O (phospholipids have P)  Triglyceride  – 1 Glycerol, 3 Fatty Acid Chains – Examples: _______________________  Phospholipid – 1 Glycerol, 2 Fatty Acid Chains – Example: __________________________  Steroid – 4 Carbon ring structure – Example: ____________________________ Lipids (cont) Lipids (cont) Lipids (cont) Lipids (cont) Lipids (cont) Cholesterol Progesterone Testosterone Uses for Lipids  ___________  Padding source and insulation  Structural  _______________ Tests for Lipids  Sudan IV Test  Newspaper Test Nucleic Acid Made of C, H, O, N and P  Monomer- ___________ Phosphate  DNA  – Sugar- Deoxyribose – Nitrogen Bases- _________  Sugar RNA – Sugar- Ribose – Nitrogen Bases- __________ Nitrogen Base DNA NUCLEOTIDE = Phosphate Purines = Deoxyribose Pyrimidines = Adenine = Thymine = Guanine = Cytosine DNA STRUCTURE A=T G=C A G T C Nucleic Acid (cont) RNA NUCLEOTIDE = Phosphate Purines = Ribose Pyrimidines = Adenine = Uracil = Guanine = Cytosine RNA STRUCTURE •Single-stranded Uses for Nucleic Acids  Genetic code ATP Structure Adenine Adenosine High Energy Bonds Ribose Phosphates ADP AMP Rechargeable Battery Protein  Made of C, H, O, S, and N  Monomer – ___________(20 found in body)  Polymer – ______________ – Amino Acids are held together by peptide bond Amino Acid Structure H H3N+ C R COOH Amino Acid Structure Polypeptide H H3N+ C R H COOH H3N+ C R H COOH H 3 N+ C R H COOH H3N+ C R COOH Uses for Proteins  Structural  ______________  Transport  _______________ Test for Protein Biuret Test Energy of Reactions  Reaction Types – Energy _____________  Rxn is spontaneous (does not mean fast)  Activation energy- energy needed to get the rxn started  At end of the rxn, energy released into environment – Energy _____________  Rxn is not spontaneous  _____________ energy- energy needed to get the rxn started  At end of the rxn, energy absorbed from environment Reaction Diagrams Enzymes __________ for rxn (speeds it up)  ___________ (reactant) enters active site of enzyme to form enzyme-substrate complex; after rxn product leaves  Enzyme is unchanged  Action of Enzymes  Speeds up rxn by _________________________________ Enzyme Reaction Rates  Rates can be affected by – ________________ – ____________ – ____________________ Dissociation of Water pH Scale Scale measuring amount of ______(really H3O+)  Ranges from _______  Below 7- _______  – Higher H+  Above 7- _______ – Higher OH-  7- ________ – Equal numbers of both
 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                            