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Transport Across Membranes Active Transport Active Transport Learning Objectives • explain what is meant by active transport, Success Criteria • Make list, table or other resource from video sources • Define and explain some uses of endocytosis and exocytosis; • Illustrate by diagrams methods of bulk transport • Complete summary sheet and questions relating to transport across membranes What is active transport? Diffusion is very important for helping to maintain the internal environment of a cell. However, molecules and ions often need to be moved across a membrane against their concentration gradient. The cell uses carrier proteins to pump these substances across the membrane, in a process called active transport. This process requires the expenditure of energy in the form of ATP (adenosine triphosphate), a molecule produced by respiration in mitochondria. Examples of active transport Active transport is used in many processes, such as the: uptake of glucose and amino acids in the small intestine absorption of mineral ions by plant roots excretion of hydrogen ions and urea by kidneys exchange of sodium and potassium ions in neurons and muscle cells. Cells that undertake active transport on a large scale have many mitochondria. Active transport Bulk Transport • http://www.youtube.com/watch?v=HndmASfmI8Y • Exocytosis in paramecium http://www.youtube.com/watch?v=U9pvm_4-bHg • Both in paramecium (with text) • http://www.youtube.com/watch?v=sn3MTYNe8mM • Phagocytosis! • http://www.youtube.com/watch?v=aWItglvTiLc Both http://www.youtube.com/watch?v=bFRoZpIAuyk Try to fill this in from your notes/group discussion DO NOT use a textbook to start Type of transport Description Diffusion Net movement of particles from an area of higher concentration to an area of lower concentration Passive. Osmosis Movement of water molecules...from an area of higher water potential to an area of lower wp Passive Facilitated diffusion Net movement...high to low concentration Carrier for large molecules, ions, polar mol Channel for smaller ions, polar molecules Passive Active Transport Endocytosis Exocytosis Movement of molecules against a conc. gradient carrier proteins. Active – requires ATP Movement large molecules/objects into a cell. Plasma membrane surrounds and pinches off. Active Movement out of a cell. Vesicle fuses with membrane and releases contents. Active What is bulk transport? When extremely large substances need to be moved across a cell membrane, bulk transport is used. The two types of bulk transport are endocytosis and exocytosis, and they involve changes to the membrane shape. Endocytosis is the bulk transport of material in to the cell, and can be split into three processes: phagocytosis, pinocytosis and receptor-mediated endocytosis. Exocytosis is the bulk transport of material out of the cell – essentially the reverse of endocytosis. Mechanisms of endocytosis Exocytosis Comparing methods of transport What’s the keyword? Multiple-choice quiz pinocytosis phagocytosis osmosis exocytosis endocytosis phagocytosis Facilitated diffusion - channel Facilitated diffusion - carrier Sodium-potassium pump