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Transcript
Centennial Honors College Western Illinois University Undergraduate Research Day 2012 Poster Presentation Evaluation of Thiosemicarbazone Containing Compounds as Inhibitors of Recombinant Cathepsin B Joseph Lucas Faculty Mentors: Lisa Wen and Jenq-Kuen Huang Chemistry The objective of this study was to test the inhibitory effects of eight different compounds on recombinant Cathepsin B, an enzyme that plays roles in processes such as protein degradation, bone resorption, and antigen processing. This protein has been found in abnormally high levels in the bloodstream of both Alzheimer’s and cancer patients, and thus furthering our understanding of Cathepsin B can help us understand its role in these diseases. In order to investigate the veracity of our Cathepsin B inhibitor candidates, recombinant pro-cathepsin B was obtained by transformation of E. coli bacteria, which were then made to produce the protein, which was then extracted and purified. After purification, the pro-cathepsin B was activated by treatment with formic acid, which cleaved off a portion of the protein, resulting in active Cathepsin B. Finally, the effectiveness of each inhibitor candidate was determined via colorimetric enzymatic assay. The results of each inhibitor candidate will be presented as an IC50 (half maximal inhibitory concentration), a measure of the effectiveness of a drug.