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THE RISK ANALYSIS Applicable A. Not Applicable Mitigating Measures Parent (Wild type) Organism 1. Domestication a) No reproduction without human aid b) Semidomesticated: wild or feral populations known c) Selfpropagating, wild 2. Agents for control a) Known b) None Known X X Cccorn can be easily controlled b available herbicide. It can not survive without the aid of man THE RISK ANALYSIS Applicable 3. Origin a) Indigenous b) Exotic c) Unknown (not sure) 4. Pest/Pathogen a) Relatives not pests/pathogens b) Relatives are pests/pathogens c) Organism is pest/pathogen itself 5. Survival under adverse conditions a) Ultra short term b) Short term c) Long term (e.g. spores, cysts, seeds, dormancy) X Not Applicable Mitigating Measures Corn is not indigenous in the Phil. But has long history of cultivation X X The only survival mechanism is the seed but germination do not last long if not properly stored THE RISK ANALYSIS Applicable 6. Distribution/ habitat a) Narrow range b) Broad range c) Unknown (limited studies) 7. Gene exchange in natural populations a) None b) Frequent c) Unknown B. Genetic Constituents 1. Donor DNA 1.1 Source of insertion X X Not Applicable Mitigating Measures Widely distributed throughout the world. Seeds of Bt corn will be properly accounted for Corn undergo both self and cross polination thus gene easily move via outcrossing. Plants will be bagged or detasseled THE RISK ANALYSIS Applicable a) Same species b) Closely related species c) Unrelated species 1.2 2. Characterizatio n a) Full b) Poor c) Unknown Vector 1.1 Source of vector a) Same species; nonpathogen b) Closely related species; non-pathogen c) Unrelated species or pathogen Not Applicable Mitigating Measures X X X The genes have been properly sequenced and identified. No unknown sequences THE RISK ANALYSIS Applicable 1.2 Vector DNA/RNA in altered genome a) Absent b) Present but non-functional c) Functional C. Phenotype of Organism 1. Fitness a) Reduced irreversibly b) Reduced reversibly c) Increased 2. Infectivity, virulence, pathogenicity or toxicity Not Applicable X X Mitigating Measures THE RISK ANALYSIS Applicable a) Reduced irreversibly b) Reduced reversibly c) Increased 3. Host range a) Unchanged b) Broadened or shifted 4. Substrate resource a) Unchanged b) Altered c) Expanded 5. Environmental limits to growth or reproduction (habitat, microhabitat) Not Applicable X X X Mitigating Measures THE RISK ANALYSIS Applicable a) Narrowed but not shifted b) Broadened or shifted 6. Resistance to disease, parasitism, herbivory or predation a) Decreased b) Unchanged c) Increased/ Unknown Not Applicable X The transgenic plants expressed the Cry1A(b) protein, which increased resistance to certain Lepidopteran insect pests including the ACB X 7. Susceptibility 7.1 to agents of control a) Increased b) Unchanged Mitigating Measures X THE RISK ANALYSIS Applicable 7.2 to absence of substrate a) Increased b) Unchanged c) Decreased Mitigating Measures X 7.3 to destruction by mechanical means a) Increased b) Unchanged c) Decreased 8. Similarity to phenotypes previously used safely a) Identical b) Similar c) Dissimilar Not Applicable X X Cargill hybrids derived from line MON 8100 are exactlty the same as the nontransfomed plants except that it has increased resistance to Acb due to C2y1A(b) protein derived from Bt THE RISK ANALYSIS Applicable D. Attributed of the Environment 1. Positive selection for GMO a) Absent b) Present 2. Possible dispersal to wild, weedy, or feral relatives a) No b) Yes 3. Vectors or agents of dissemination or dispersal (mites, insects, rodents, birds, humans, machines, wind, water, etc.) Not Applicable Mitigating Measures THE RISK ANALYSIS Applicable a) Absent b) Present but controllable c) Present and uncontrollable 4. Direct involvement in basic Ecosystem processes (e.g. nutrient cycling) a) Not involved b) Marginally involved c) Involved/key species Not Applicable Mitigating Measures THE RISK ANALYSIS Applicable 5. Range of environments for use or testing; potential geographical range a) Very restricted b) Restricted c) Broad/ widespread 6. Simulation of test conditions a) Can simulate realistically b) Very difficult to simulate realistically Not Applicable Mitigating Measures THE RISK ANALYSIS Applicable 7. Public access to test site a) Tightly controlled b) Controlled c) Uncontrolled 8. Effectiveness of monitoring and mitigation plans a) Proven effective b) Likely to be effective c) Untested or unlikely to be effective Not Applicable Mitigating Measures