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Organelle Review Plasma Membrane Surrounds The Cytoplasm Which contains Golgi Apparatus Mitochondria Ribosomes Nucleus Protein Synthesis and Secretion • Ribosomes attach to the E.R. and make protein • Protein is then sent to the Golgi Apparatus • The G.A. packages it up and sends it off to the Plasma Membrane where it can be released from the cell. Ribosomes –> E.R. –> Golgi A. –> Plasma Membrane Why do Organelles have Folded Membranes? • Provides a larger surface area • Allows for cell processes to be more efficient and effective • Forms interconnected compartments Basically it gives the organelle more space to do work! Are all Cells Created Even? “How come plant cells have chloroplasts, I want one of those! That’s Booty!” Only in Animal Cells • Lysosomes – contains enzymes that digest proteins, food, and other such things. • Centrioles – paired structure that assists during cell reproduction Only in Plant Cells • Plastids –produces food for the cell through a process called photosynthesis (chloroplast, chromoplast, leucoplast) • Cell Wall – protection and support – Also found in algae, fungi, and bacteria • Dictysomes – responsible for cell wall formation (collection and packaging center) Bacterial Cells (prokaryotic) • Single Celled Organisms • Lack a nucleus and other membrane bound organelles Copy picture from board Membrane Properties • Gate Keeper – controls what enters and leaves the cell (outer boundary) • Selective Permeability – only certain substances can cross the membrane – Small vs. Large – Neutral vs. Charged Membrane Structure • Lipid Bilayer – two layers of phospholipids acts as barrier and boundary • Phospholipid – 2 fatty acid tails and a phosphate head • Tails are non polar, don’t like water • Heads are polar, face inside and outside of cell where water is Membrane Structure Continued • Cholesterol – bind and restrict movement of lipid bilayer; allows for strength and flexibility • Proteins – some pass through bilayer, while some are only on surfaces – Function regulation, enzymes, markers for chemicals, infection, and recognition Fluid Mosaic Model Fluid - membrane is flexible Mosaic - forms patterns on the membrane surface Membrane Function Diffusion Osmosis Passive transport Active transport Endocytosis Exocytosis DIFFUSION • Random movement of particles from high concentration to low concentration • [high] to [low] • Diffusion occurs across membranes until equilibrium (homeostasis) is reached • Dynamic equilibrium – particles are evenly spread, equal [ ] , but still moving OSMOSIS • Diffusion of H2O across a selectively permeable membrane • Osmotic conditions have 3 options: Isotonic, Hypotonic, Hypertonic How many of you have every killed a plant? How? Over watering or Under watering? • Isotonic – same [ ] of solutes inside as outside cell, not net change • Hypotonic – low solutes/ high H2O outside cell; H2O moves in (Animal cells burst; Plant cells swell) • Hypertonic – high solutes/ low H2O outside cell; H2O moves out • Animal cell shrinks • Plant cell contents shrink (loss of turgor pressure) Think… when you are hyper, you want to move out of your seat! Osmosis State Isotonic H2O [H2O] Movement Inside cell [H2O] Outside cell none [same] [same] Hypotonic Into the cell [Low] [High] Hypertonic Out of the cell [High] [Low] ` Osmosis State Isotonic H2O Solute [ ] Movement Inside cell Solute [ ] Outside Cell none [Same] [Same] Hypotonic Into the cell [High] [Low] Hypertonic Out of the cell [Low] [High] PASSIVE TRANSPORT • No energy is required to move particles through the cell’s plasma membrane • Facilitated Diffusion – Protein required to move particles through membrane – Move down [ concentration] gradient by random movement – Channel proteins vs. carrier proteins – How glucose (sugar) enters the cell ACTIVE TRANSPORT • Move substances from [low] to [high] • Requires: – Cell to use energy – Transport protein (pump) = each pump is specific for a certain substance • 2/3 of your energy used to actively transport Sodium and Potassium across membranes ENDOCYTOSIS (endo = in) • Process in which the plasma membrane engulfs substances from the environment • Pinocytosis – “cell drinking” large drops of liquid. • Phagocytosis – “cell eating” large particles are engulfed into a vesicle for digestion – Ex: Amoeba’s and White Blood Cells (Immunity) EXOCYTOSIS (exo = exit) • Process of removing large particle or volumes of liquid • Excretion – removal of waste products • Secretion – removal of cell products needed elsewhere • Requires vesicle and energy