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The Cell Cycle Mitosis Pg. 204- 210 in your book Quick Facts About the Cell Cycle The cell cycle is common to all living things It is a sequence of growth and division of a cell Two major periods of the cell cycle: I. Interphase II. Mitosis Interphase The time between cell division when a cell grows and replicates (makes copies of DNA) The majority of a cell’s life is spent in interphase -Growth Phase I (G1): Proteins are made, the cell grows and organelles increase in size and number -Synthesis Phase (S)- DNA replicates; the purpose of this is so that the new cell can have the exact genetic copy -Growth Phase (G2)- more proteins are made, centrioles used for cell division are copied and being to make spindles Cell Cycle Mitosis -A continuous process that has four phases of the division of the nucleus -This is the process where two daughter cells are formed, each containing a complete set of chromosomes. Prophase The first stage of Mitosis The longest mitotic phase 1. Chromosomes appear because chromatin is condensing -What is condensing? Coiling and getting thicker 2. Centrioles move to the poles of the cell 3. Centrioles begin to form spindle -these attach to the centromeres which hold together the sister chromatids 4. Nuclear membrane begins to disappear 5. Nucleolus disappears Another picture of prophase Metaphase Chromosomes become attached to the spindle fibers by their centromeres Chromosomes line up across the middle of the cell -Also known as the equator Another picture of metaphase Anaphase Chromosome separate and one sister chromatid of each pair moves to each pole of the cell Another picture of anaphase Telophase Chromosomes arrive at opposite ends of the cell Two nuclei form It’s the reverse of prophase More telophase! Cytokinesis Finally the end! This is the division of cytoplasm This happens in two different ways 1. In animal cells- the cytoplasm and plasma membrane pinch in along the equator to create cleavage furrow 2. In plant cells- cell plate forms along the equator -Plants have a rigid cell wall so the plasma membrane does not pinch in -A cell plate is laid down across the equator and a cell membrane forms around each cell and new cells walls form to complete the separation. Plant Cell Cytokinesis vs. Animal Cell Cytokinesis Plant Cell Animal Cell cytokinesis begins with a series of vesicles that form at the equator of the cell, which subsequently join until the cell is divided in two. cytokinesis starts with a cleavage furrow or indentation around the middle that eventually pinches in, dividing the cell in two Cell Cycle Summary Interphase – – – G1 stage- Growth & development of the cell Protein synthesis S-phase- Chromosome replication via DNA synthesis G2 stage- Growth & development Organelle Replication Mitosis Prophase- Replicated chromosomes condense , Spindle fibers form Metaphase- Replicated chromosomes align at center Anaphase- Sister chromatids separate Daughter chromosomes move to poles Telophase- New nuclear membranes form Spindle fibers disappear Cytokinesis- Cell divides into two daughter cells Now how am I supposed to remember all of this? Remember PMAT -Prophase -Metaphase -Anaphase -Telophase (yes, I know it is simple but it also helped me remember!) -Just make sure to remember that Interphase comes before PMAT and Cytokinesis comes at the end! Now the fun begins!! You get to make a cell cycle booklet! -You are going to make a booklet of the cell cycle, making sure to include interphase and mitosis -Interphase should include the 3 stages (draw and briefly describe events for G1, S and G2 phases) -Draw, label and briefly describe the stages of mitosis (If you draw it, you must label it!) -The last page of your booklet should show cytokinesis for both animal and plant cells -Structures to be labeled: Nucleolus, Centriole, Spindle Fibers, Daughter Cells, Chromatin, Centromere, Cleavage Furrow, Nucleus, Sister chromatids, Cell plate Things to really pay attention to that you will be graded on! -Accuracy -Neatness -Making sure all requirements are met above!