* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download ci sono le basi per ri-valutare le specifiche delle diete
Survey
Document related concepts
Transcript
Vitamin requirements: is there basis for re-evaluating dietary specifications? From Vitamin requirements: is there basis for re-evaluating dietary specifications? S. LEESON1, adapted and modified by M. Di Benedetto2 1 Department of Animal & Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 E-mail: [email protected] 2 DVM, Consulenze Aziendali Zootecniche studio associato e-mail: [email protected] During the last 40 years there have been few studies aimed to estimate the vitamin requirements in poultry, so that consequently the NRC values (1994) still are the standard reference. table 1: Vitamin requirements NRC (1994) compared to commercial and field recommendations (Leeson, 2007) Layers (ingestion 100gr/head/die) Vitamin NRC A (UI) D3 (UI) 3.000 300 E (mg) K (mg) B12 (µg) Biotin (µg) Choline (mg) Folic acid (mg) PP (mg) Pantot. Ac.(mg) B6 (mg) B2 (mg) B1 (mg) 5 0,5 4 100 1.050 0,25 10 2 2,5 2,5 0,7 1 Leeson and Summers1 8.000 3.500 Leeson and Summers (2005) DSM (2006) Biotin for Maize-based diets 2 50 3 10 100 400 1 40 10 3 5 2 DSM2 8-12.000 2.5003.500 15-30 2-3 15-25 100-150 300-500 0,5-1 20-50 8-10 3-5 4-7 1,5-3 Broilers starter NRC Leeson and DSM2 Summers1 1.500 200 8.000 3.500 8-12.500 3-5.000 10 0,5 10 150 1.300 0,55 35 10 3,5 3,6 1,8 50 3 12 100 400 1 40 14 4 5 4 150-240 2-4 15-40 150-300 300-600 1-2 50-80 10-18 3-6 7-9 2-3 VITAMIN REQUIREMENTS NRC 1994 These requirements are quite far from the present ones commercially used, since: • Their elaboration is long time dated, as it goes back to the period between 1990 and 1994. • They are based on studies appeared on sector books and magazines issued during the last 40 years, without taking into considerations the enormous progress attained by genetics, nutrition, and breeding techniques in recent years. • Several trials, among those ones considered, have been carried out by using purified or semi-purified diets, characterised by digestibility definitively higher than in the commercial diets. (i.e. soy isolated protein or casein as protein and amino acid source; and dextrose, starch, and saccarose as energy source). • Considered the difficulty that such diets present for pelletisation, the physical form of feed utilised in these trials basically was as meal, with no homogeneous characteristics, as for particle size, colour and consistency. Moreover it has to be considered that most feed for poultry is nowadays manufactured in pellets or crumbles, necessary to reach the performances foreseen by the genetics producers. • Most of the studies taken into consideration by NRC (1994) about vitamin requirements are based on the observation of presence/absence of deficiency status and/or basis yielding parameters such as eggs weight, growth and conversion rate, without considering health data, slaughtering data, final product organoleptic characteristics. A revision of such information indicates that several figures come from estimation, or are taken from similar species, or as in broiler breeders simply not available. CHANGES IN THE GENETIC POTENTIAL The present interest in the vitamin level formulation in diets for poultry is due to the observation that improvements in the genetic potential of layers and broilers impose a re-evaluation of the requirements since the conversion rate improvements (decrease of the feed intake per product unit) has implied a continuous decline of vitamins ingestion related to eggs weight or produced meat. Such decrease is estimable around 1% in layers, and around 0.6 – 0.8% in broilers. The following table (n. 2) shows this phenomenon throughout the last 20 years in layers (white), broilers, turkeys, taking as an example vitamin E: table 2: Ingestion of vit. E per production unit, diets containing 20 UI vit. E/Kg (Leeson, 2007) Layers (1Kg) Broilers (2Kg) Turkeys (14Kg) 1985 2005 ∆/year 2,7 UI/egg 2,1 UI/egg -1,1% 40 UI/Kg growth 55 UI/Kg growth 34 UI/Kg growth 48 UI/Kg growth -0,8% -0,6% This lower ingestion of vitamins represents the basis of performance improvements when feed containing vitamin levels higher than normal are administered. For several vitamins what was described in 1993 as “high level” of supplementation nowadays is described in a better proper way as “modest” or “low level”. In layers early maturity reached at a lower body weight imposes a further variable on the vitamin diet specifications. Most feeds for layers nowadays are formulated on the basis of the daily ingestion. Considering the expectation of lower ingestions, the level of nutrients in the diet, such as amino acids and calcium have been invariably increased. However the adjustment of the vitamin levels on ingestion basis remains unusual. At every production stage, the ingestion of Vitamin D3 represents a critical factor for the achievement of both egg shell and skeleton integrity quality. Table 3 shows how the ingestion of vitamin D3 is affected by the ingestion of feed at the beginning of the yielding phase: table 3: Feed specification for Ca, dig. P and vitamin D3 necessary to keep daily ingestions of: Ca 4g, P dig. 0,4g, vit. D3 330 IU in white layers at the beginning of yielding (Leeson, 2007) Age Yielding (%) Ingestion (g/gg) Ca (%) dig. P (%) vit.D3 (IU/Kg feed) 18 19 20 22 24 1 20 50 90 95 5,30 5,00 4,70 4,40 4,20 0,53 0,50 0,47 0,44 0,42 4.400 4.125 3.880 3.660 3.470 75 80 85 90 95 VARIATION OF THE IMPORTANCE OF THE PARAMETERS USED TO EVALUATE VITAMIN REQUIREMENTS Our expectations on the role of various nutrients have changed through time. As previously mentioned most vitamin requirements expressed by NRC (1994) have been evaluated through criteria bond to yielding parameters that nowadays honestly appear to be simplistic. At present these evaluation criteria have been extended and the nutritionist role is to evaluate the requirements to reach such targets in the most profitable way in terms of cost/benefit. Table 4 underlines the evaluation criteria that are now often applied to requirements of vitamins and other nutrients. table. 4: Evaluation criteria for vitamins (Leeson, 2007) NRC (1994) Present additional criteria Layers n° of eggs eggs weight egg shell quality egg composition solid part immunity effect wellness Broilers weight convertion rate mortality carcass characteristics breast yield microbiologic quality Immunity effect At present the potential on nutrients such as vitamins to act as substances “nutriceutical” is very important, both in terms of wellness and health in poultry, and in particular of capacity of immune response. In particular there is the suspect that virtually all data used to determine the vitamin requirements NRC (1994) come from studies in which animals were in optimal health status, not under immunity stress. In fact in most of these studies there has been no need to effect any vaccination on animals under testing. REQUIREMENTS TO SUPPORT GENERAL HEALTH OF ANIMALS The effect of vitamins of health and in particular on the immune response in animals is one of the elements of most impact on the re-evaluation of the vitamin addition in diets. • Sell et al. (1997) assert that the levels of vitamin E from NRC (1994) are probably sufficient in growing turkeys with no pathologies, but previous studies (Soto-Salanova e Sell, 1996) demonstrate that deposits of atocopherols in poults are low and decline rapidly during the first 14 days of life. The administration of vit. E per parenteral route or added to feed (150mg/kg), although it did not eliminate the phenomenon, it weakened it in a significant way. The decrease of vit. E in liver of stressed poults did not determine effects on their health, but after administration of vit. E less sensitiveness to haemolysis of red cells was highlighted. • High levels of vitamin E in feed can support the egg production in layers under heat stress. Through heat stress induced at 32°C Bollengier-Lee et al. (1998) demonstrated a higher egg production in layers fed with 500 vs. 10 mg vitamin E/kg. In other trials the effect, although less evident, was always confirmed as increase of calcium, vitellogenine, and VLDL in the blood of tested animals. Moreover these animals have always recovered more rapidly from short-term heat stress. These authors concluded that the extra vitamin E helped these stressed animals through the improvement of the hepatocytes membrane integrity, and/or through Estradiol activity modulation or concentration. Similar results were obtained by Whitehead et al. (1998) by using the same levels of vitamin E (500 mg/kg feed); the level of vitamin E in plasma was linearly related to the one in the diet, and it returned to basic levels four weeks after having removed the extra vitamin E from the diet. These authors suggest that the antioxidant function that vitamin E in general exerts has an effect on the vitellogenine level (that represents yolk most important precursor) in blood. The control of the vitellogenine level in plasma and in liver suggests that heat stress does not compromise the actual vitellogenine production, but it compromises its release in the general circulation. • In recent time several studies have been carried out also on vitamin D3: A study carried out by Atencio et al. on broilers fed with feed containing 3200 IU vitamin D3 highlighted higher weights when born from breeders that received at least 4000 IU vitamin D3/kg feed. Whitehead et al. (2004) suggests that requirements for modern broilers is remarkably higher than that suggested by NRC (1994) and even higher than the legal limits fixed by the E.U.. In two trials better growth have been obtained by feeding the animals with feed containing 10,000 IU vitamin D3 compared to others fed with diets containing also a high level of 6000 IU vit. D3. Tibia strength resulted to be proportional to the feed level of vitamin D3, whereas tibia dyschondroplasia incidence was lower with 6,000 IU, and at zero with 10,000 IU. REQUIREMENTS TO FAVOUR THE IMMUNE RESPONSE Several studies have been carried out on the effect of a vitaminic supplementation (especially of vitamin A, D3, E, and C) on the immune response in broilers, layers, breeder. In general any response to vitamin administration is registered at 10 times the level suggested by NRC (1994) and often equal to 2-3 times the one commercially used. • Vitamin E and its dosage concerned most of recent studies. Sijben et al. (2002), starting from the observation of the polyunsaturated fat acids (PUFA) effect on the immune response they have investigated the possible synergism between PUFA and vitamin E on the immune response. By using a feed containing 80 mg/kg vitamin E and 10% PUFA no apparent interaction has been observed or any beneficial effect on PUFA accumulation in tissues or on their activity (in this regard it is opportune to remark how synergism between vitamin E and PUFA, documented in several studies, is probably due to the vitamin proper antioxidant effect and that therefore it occurs only in case of the fatty acids oxidation (MDB). Puthpongsiriporn et al. (2001) studied the effect of vitamin E on lymphocytes proliferation and on the oxidative status in layers under heat stress and fed with feed containing levels of vitamin E up to 65 mg/kg. Heat stress was simulated through daily fluctuations of temperature 21 to 35°C, or through constant temperature of 35°C. The 65mg level increased the mass of produced eggs in animals under heat stress, but not on control. An increase of lymphocytes proliferation was also found after administration of S. typhimurium in groups fed with 45 and 65 mg compared to that with 25 mg. Table 5: effect of various levels of vitamin E on the mass of eggs produced by layers under heat stress (Puthpongsiriporn et al., 2001). Moreover an effect on some qualitative characteristics of eggs was also observed: Table 6: effect of various levels of vitamin E on the quality of eggs produced by layers under heat stress (Puthpongsiriporn et al., 2001). The figures shown in previous table confirm the hypothesis that in condition of heat stress levels slightly higher than the commercial ones increase the yolk weight through an increase of the circulating vitellogenine. In a further study, besides confirming previous data, the same authors also found a synergetic (or additional) effect between vitamin E and vitamin C both on the number and on the mass of produced eggs. Table 7: effect of various levels of vitamin E in combination or not with vitamin C on productive parameters in layers under heat stress (Puthpongsiriporn et al., 2001). From the point of view of the antibody production Leshchinsky (2001) at the end of numerous studies highlighted that the most effective level of vitamin E was 50 mg/kg feed, level beyond which the immune response is no longer linear. A cause of variability in the immune system response at various dosages of vitamin E (and probably of other vitamins) is to be found in the different response by the various genetic kinds. Boa-Amponsen et al. (2000) used three different pure broiler lines to study the response to two diets characterised by a different content of vitamin E (10 vs. 300 IU/kg). Two out three lines the higher level of vitamin E helped to keep a higher response of primary and secondary antibodies after injection of heterologous red cells, whereas in the third genetic line the response was even negative. Even the effect of IgF and IgM showed the same line-depending performance. The authors concluded that vitamin E improves the immune system phagocytal capacity despite it has a limited effect on the mediated-cell immunity. However all effects seem to have an element bond to genetics, and this can explain the variability in the immune responses to vitamin E. • Vitamin D3 activity is well known on ossification processes, but it was also studied for its implications on the immune system. Aslam et al. (1998) proved that inadequate levels of this vitamin in the diet compromise the immune response. In fact the response to an inadequate diet was a reduced activity of basophiles as response to the cutaneous hemagglutinin. Also the thymus weight per body weight unit, and the macrophages activity resulted to be depressed. The authors conclude that the deficiency of vitamin D3 interferes negatively on the immune response of mediate-cell type. Another series of studies have been carried out on the immune response compared to vitamin 25-OH D3. Such studies did not highlighted any effect bond to the source of vitamin D, but have clarified that levels of 2000 (layers) and of 4000 (broilers and turkeys) IU/kg feed are necessary for a normal immune system functioning. NEOFARMA SUGGESTION TO OPTIMISE THE VITAMIN SUPPLEMENTATION ( Select "NEWS" )