Download Κατερίνα Χαρβάλου 1. Σπουδές-δραστηριότητες: 1974

Κατερίνα Χαρβάλου
1. Σπουδές-δραστηριότητες:
1974-1982
1984-1988
1991
1996
Πτυχίο Ιατρικής Σχολής Πανεπιστημίου Aθηνών.
Διδακτορική διατριβή Ιατρικού τμήματος Παν/μίου Γενεύης με
θέμα: Μελέτη Campylobacter jejuni/coli : Κλωνοποίηση και μερική
έκφραση αντιγόνων εξωτερικής μεμβράνης Campylobacter jejuni σε Ε.
Coli.
Ειδικότητα ιατρού μικροβιολόγου
Τίτλος εξειδίκευσης στην κλινική μικροβιολογία (υπ υγείας)
 Υπεύθυνη έκδοσης: Μapping the Maze: Getting more women to the top in
research. European Commission, (2008), ISBN 978-92-79-07618-3
 Μέλος ιατρικών και
Ευρωπαική Επιτροπή
επιστημονικών
εταιρειών,
εμπειρογνώμονας
στην
 Κριτής στα περιοδικά Biotechnology and Bioengineering, Microbial Ecology,
Βiofouling.
2. Τομείς ερευνητικών ενδιαφερόντων:
Διαγνωστική και μοριακή διαγνωστική των λοιμώξεων. Αντιμικροβιακή αντοχή και
νέοι αντιμικροβιακοί παράγοντες (πεπτίδια) και συστήματα αποδέσμευσης (delivery
systems). Φύλο, βιοπαθολογία λοιμώξεων και κλιματικές αλλαγές.
3. Συγγραφή βιβλίων:
 ΔΙΑΓΝΩΣΤ ΙΚΗ Μ ΟΡΙΑΚΗ ΜΙΚΡΟΒΙΟΛΟΓ ΙΑ , Αικατερίνης Χαρβάλου,
Ιανουάριος 1998. Ιατρικές Εκδόσεις Π .Χ. Πασχαλίδη.
 ΣΥΝΟΠΤ ΙΚΗ ΚΥΤΤΑΡ ΙΚΗ ΒΙΟΛΟΓ ΙΑ –Ε ΙΣΑΓΩΓΗ ΣΤΗ ΜΟΡΙΑΚΗ
ΒΙΟΛΟΓ ΙΑ ΤΟΥ ΚΥΤ ΤΑΡΟΥ . Iατρικές εκδόσεις Π.Χ. Πασχαλίδη,
2002, Αικ. Χαρβάλου, Διομ. Πηγής, Φιλίππου, Τρίγκας .
 Πρωτόκολλα κλινικής Μικροβιολογίας. Ιατρικές
Πασχαλίδη, 2007 .
4. Διδασκόμενα μαθήματα:
1. Kλινική μικροβιολογία
2. Μεθοδολογία έρευνας
3. Κυτταρική/Μοριακή βιολογία
Eκδόσεις Π.Χ.
5. Ενδεικτικές-πρόσφατες δημοσιεύσεις:
1.
Shehabi A.A., Z. M. Awad, M. Al-Ramahi , E. Charvalos and L.F. AbuQattouseh Detection of Mycoplasma genitalium and Trichomonas
vaginalis Infections in General Jordanian Patients. Αm J Inf Dis (2008),
In press
2.
Dakic I, Lilakos D, Svabic-Vlahovic M, Christofilipoulou S, Poggas N and
E Charvalos. Integron-associated antimicrobial resistance in isolates of
members of Enterobacteriaceae causing community-acquired urinary
infections. Microb Ecol In Health and Dis (2007) 19:201-206.
3.
Alexopoulos, A. Voidarou, C. Stefanis, C. Papadopoulos, I. Vavias, S.
Tsiotsias, A. Kalkani, E. Charvalos, E. Bezirtzoglou, E. Antibiotic
resistance profiles and integrons in Enterobacteriaceae from the
riverside of Evros-Ardas with respect to chemical and waste pollution.
Microb Ecol in Health and Dis. (2006), (18); Numb 3-4, pp 170-176.
4.
Vassilakopoulou A, Daikos GL, Kosmidis C, Charvalos E, Passiotou M,
Avlamis A and G Petrikkos. Class I integrons in Enterobacteriaceae from
animals hospitalized and non-hospitalised patients. Archives of Hellenic
Medicine (2006) 23 (1): 45-51.
5.
Charvalos, E., Tzatzarakis M., Van Bambeke F, Tulkens M., Tsatsakis
A. M., Tzanakakis G., and Marie-Paule Mingeot-Leclercq. Water-soluble
amphotericin
B-polyvinylpyrrolidone
complexes
with
maintained
antifungal activity against Candida spp. and Aspergillus spp. and
reduced hemolytic and cytotoxic effects. J Antimicr. Chemother. (2006)
57, 236-244.
6.
Petrikkos GL, Christofilopoulou SA, Tentolouris NK, Charvalos EA,
Kosmidis CJ, Daikos GL.Value of measuring serum procalcitonin, Creactive protein, and mannan antigens to distinguish fungal from
bacterial infections. Eur J Clin Microbiol Infect Dis. (2005), 24(4): 272-
6. Επικοινωνία
+30 210 5385697, +302105385610
FAX 210 5385866
email: [email protected]
Biographical sketch
Ekatherina Charvalos
Email address: [email protected],Athens: 51, Pipinou str,
112 51. Tel 0030210 6045078
Athens Greece,
Dr: Ekatherina Charvalos is a medical doctor, specialized in biopathology,
clinical and molecular microbiology. She is a professor of microbiology,
molecular biology and research methodology at the Technological Institution
of Athens. She has been detached at the EU Commission DG Research,
Science in Society -Scientific Culture and Gender issues Unit L4 for two years
(2006-2008). She is m arried to Dr George Assonitis and is mother of a unique
child.
Academic Appointments and professional positions:






Research associate at the Department of cellular and molecular
pharmacology, Université Catholique de Louvain, Faculté de Médecine,
Ecole de Pharmacie (sabbatical, for one academic year) - (2003-2004).
Since April 1999 she is a Research associate at the Infectious
Diseases Department, University of Athens, Medical School. (Dir. Ass.
Prof. Dr. G. Petrikkos).
Since November 1997 she is a Prof . of Microbiology/Mol Biology,
School of Health Sciences, Medical Laboratories Division.
Technological Institution of Athens.
Visiting Scientist Tufts Medical School, Center for Adaptation Genetics
and Drug Resistance. Dept. of Molecular Biology & Microbi ology
(1996-1997) working on Multidrug efflux pumps in gram - negative
bacteria. Assistant Professor of Bacteriology University of Crete
Medical School (5/1996 - 11/98)
Research Associate -Lecturer of Bacteriology. University of Crete,
School of Health Sciences (1/1991 - 5/1996),
Associate Professor of Parasitology, Mycology and Immunology.
Technological Institute of Athens, Greece. Division of Public Health
and Medical Laboratories (1990 -1993).
Awards, Honors, grants, research projects and affiliations:
Dr Ekatherina Charvalos got s even awards for research on mechanisms of
resistance in enterobacteriaceae and Campylobacter jejuni, mycoplasmas,
and efflux pump.
Accomplished research: Fifteen research projects funded by the EEC-Greek
government (5 as a c o-ordinator), (10, as a participant). Projects focused on:
1. New antifungal agents of controlled release (Amb -PVP, nystatin-PVP or
sorbic acid-PVP), 2. Spread of integrons in nosocomial, non -hospitalised
patients, environmental and veterinary strain, 3. D etection of genital
mycoplasmas and their antibiotic resistance profile (tri -lateral agreement
Greece-France-Serbia), 4. Detection by PCR of M. genitalium in clinical
samples (bi-lateral agreement Greece -Romania). 5. Non -invasive methods
for the detection of pesticides in human hair, One prize (Empeirikion
Institution) for research activities at the University of Geneva.
Dr Ekatherina Charvalos is an Expert at the Ministry of Development for
Medical Microbiology testing, Expert –evaluator 6th framework pr ogramme,
DG Research. FP6 -2003-LIFE SCIENCES HEALTH -I, CRAFT, STREP.
Research for the benefit of the SMEs. DG SANCO (EAHC) Call 2009. Member
of the Hellenic Society of Microbiology, APUA, ( The alliance for the prudent
use of antibiotics) and the American Society for Microbiology.
She is offering her services as a Reviewer in peer -reviewing journals
(Biotechnology and Bioengineering, Microbial Ecology, Biofouling).
Education
Certificate of specialization in Clinical Microbiology. (March 1996), Diploma
of Specialization in Medical Microbiology (1991),
Doctoral Dissertation - Degree of excellence. Doctorat en Médecine. Centre
Medical Universitaire de Genève. Department of Genetics & Microbiology
(1989), she is a doctor in medicine since 1982, (Ptychion) - University of
Athens Medical School, Athens, Greece.
Medical Training: She has been resident in biopathology at Hopital Cantonal
Universitaire de Geneve. Laboratoire Central de Bactériologie (1984), at the
Hospital of the "Sainte Providence, I Pammakaristo s", Athens, 1983,
assistant at the regional hospital of Pyrgos, Greece (1982 -1983), and
"Assistant -medecin" and Membre légitime de la recherche et de l'
enseignement. C.M.U.- H.C.U.G. (Centre Medical Universitaire de Geneve):
Departement de Genetique et Microbiolgie. (Doctoral studies 1985 -1988)
Post-Doctoral Studies and Training (most important) :
Research fellow, University of Geneva, CMU, Dept. of Genetics and
Microbiology (2002). Sesame workshop on Bioinformatics, Sabanci
University, Istanbul (2001 ), ASM General Meetings (Continuing Medical
Education, USA, credits), Laboratory course on PCR application on the
diagnosis of mycobacterial, HIV and HBV infections, Roche Diagnostic
Center, Nicosia Cyprus (1996), Seminar on "Epidemiology, Health &
Environment",Institute Merieux and the University of Savoy, France. FEMS
laboratory course on the immunochemical and molecular genetic analysis of
bacterial pathogens and their virulence determinants. Trinity College, Dublin,
Ireland (1989).
Publications (scientific articles -congress participation) She has published
about 15 scientific articles in peer -reviewed journals on new antifungals of
controlled release, antibiotic resistance efflux mediated, spread of integrons
and multidrug resistance in gram -negative strains and she participated in
more than 80 congresses.
Patent Certificate: Dr. Ekatherina Charvalos is participating (20%), to patent
Certificate no 20000100176, 25/5/00, Greek Industrial Property Organisation.
The above mentioned certificate is protecti ng PVP -sorbic acid, PVP nystatin, and PVP-amphotericin B complexes.
Bibliography Dr E Charvalos is the author of a) “Diagnostic Molecular
Microbiology” (1997) Ekatherina Charvalos, Paschalides, Ed. Athens
Greece, b) “Synopsis of cellular biology: an in troduction to molecular
biology” (2001). E. Charvalos., al., Paschalides ed. Athens, Greece, c)
“Clinical Microbiology protocols”, Paschalides Medical ed (2007).
Languages and citations: She fluent in Greek, French, English
Citacions: more than 600 in sc ientific literature and EU policies (as national
detached national expert at the EU Commission, DG RTD, Unit L4 Scientific
culture and gender issues).
Research and policy areas of interest: a) new polymeric forms of
antibiotics: in vitro activity, cytotox icity, bio-compatibility and applications in
human and animals b) diagnostic molecular microbiology and molecular
epidemiology c) Policies for preventing of antibiotic resistance, d) Genital
Mycoplasmas: impact on human health - antibiotic resistance f) Bac terial
antibiotic resistance integron or efflux -mediated, e) Dr Ekatherina Charvalos
is interested in ethical and gender issues in research and the EU Science
in Society policies and international cooperation, f) gender, health and
environment in infectiou s diseases.
Publications
(Most relevant)
1.
Adamopoulou M., Kalkani E., Charvalos E., Avgouatidis D.,
Haidopoulos
D.,
Yapidjakis
C.
Comparison
of
cytopology,
colposcopy, HPV typing and biomarker analysis in cervical
neoplasia. Antricancer Res . (2009) 29(8): 3401 -9.
2.
Dakic I, Vassilakopoulou A, Charvalos E, Passiotou M, Petrikkos G. Multidrug
resistance and integrons in Escherichia coli isolated from chicken in Greece.
Acta Veterinaria (2009) accepted.
3.
Shehabi, A.,
Z.M. Awwad, M. Al-Ramahi, E. Charvalos and L.F. Abu-
Qatouseh. Detection of Mycoplasma genitalium and Trichomonas vaginalis
Infections in General Jordanian Patients. Am Journal of Infect Dis 5 (1): 7-10,
(2009)
4.
Dakic I, Lilakos D, Svabic -Vlahovic M, Christofilipoulou S,
Poggas N and E Charvalos. Integron -associated antimicrobial
resistance in isolates of members of Enterobacteriaceae causing
community-acquired urinary infections. Microb Ecol. In Health and
Dis. (2007) 19:201-206.
5.
Vassilakopoulou A, Daikos GL, Kosmidis C, Charvalos E,
Passiotou M, Avlamis A and G Petrikkos. Class I integrons in
Enterobacteriaceae
from
animals
hospitalized
and
non -
hospitalised patients. Archives of Hellenic Medicine (2006) 23 (1):
45-51.
6.
Charvalos, E., Tzatzarakis M., Van Bambeke F, Tulkens M.,
Tsatsakis A. M., Tzanakakis G., and Marie -Paule Mingeot Leclercq.
Water-soluble
amphotericin
B -polyvinylpyrrolidone
complexes with maintained antifungal activity against Candida
spp. and Aspergillus spp. and reduced hemolytic and cytotoxic
effects. J Antimicr. Chemother. (2006) 57, 236-244.
7.
Petrikkos GL, Christofilopoulou SA, Tentolou ris NK, Charvalos
EA,
Kosmidis
procalcitonin,
CJ,
Daikos
C -reactive
GL. Value
protein,
and
of
measuring
mannan
serum
antigens
to
distinguish fungal from bacterial infections. Eur J Clin Microbiol
Infect Dis.(2005), 24(4): 272-5.
8. Alexopoulos A, Voidarou C, Stefanidis C, Papadopoulos I,
Vavias S, Kalkani E, Charvalos E and E Bezirtzoglou. Antibiotic
resistance profiles and integrons in Enterobacteriaceae from the
riverside
Evros -Ardas
with
respect
to
chemical
pollution
(extended abstract) Microb Ecol. In Hea lth and Dis. (2006) vol
18: 170-176.
9.
Christofilopoulou,
S.,
E.
Charvalos,
G.
Petrikkos.
Could
procalcitonin be a predictive biological marker in systemic fungal
infections? Study of fourteen cases. European Journal of Internal
Medicine, (2002) 13:493-495.
10. Charvalos E., M. Tzatzarakis, M. Tsatsakis & G. Petrikkos
Controlled release of water soluble polymeric complexes of sorbic
acid with antifungal activities Appl. Microbiol.& Biotechnol . (2001)
57: 770-775.
11. Charvalos E., Tsatsakis A., Tzatzarakis M, Dola psakis G.,
Stiakakis J. New nystatin polymeric complexes and their in vitro
antifungal evaluation in a model study with Fusarium oxysporum.
Mycopathologia, (2001), 153: 15 -19.
12. Charvalos, E., Karoutis, A.D. Antimicrobial activity of ArF
excimer Laser radia tion in gram- negative bacteria. Appl.
Microbiol. & Biotechnology , (2001), 56(1-2): 243-8.
13. Tzatzarakis, A. Tsatsakis, Charvalos E., Vakalounakis, D.
Comparison of in vitro activities of amphotericin, clotrimazole,
econazole, miconazole and nystatin agai nst Fusarium oxysporum.
J. Env. Health. (2001), 36(3): 331-40.
14. Hαdjichristodoulou
C.,
Papatheodorou
C.,
Sotiriades
E.,
Panagakos G., Charvalos E. Tselentis. Epidemiological study of
brucellosis in eight Greek villages using a computerised mapping
programme. Eur. J. of Epidemiol. (1999), 15 (7): 671-680.
15. Charvalos, E., Peteinaki, E., Spyridaki, I., Manetas, S., and
Tselentis, Y. Detection of ciprofloxacin resistance mutations in
Campylobacter
jejuni
by
non-radioisotopic
Single
Strand
conformation polymporphism and direct DNA sequencing. J. Clin.
Lab. Analysis Vol. X, (1996), 10 (3): 129 -133.
16. Karoutis A. D., Z. Guiti, E. Charvalos, I. Tselentis and E.
Helidonis. Bactericidal effect of ArF Excimer laser radiation. I.
Gramnegative bacteria. Lasers in the Life Science. (1996), 7(1)
pp. 1-12.
17. Charvalos, E., Tselentis, Y., Hamzehpour, M.M. and Pechere,
J.C.,
Evidence
for
an
efflux pump
in
multidrug
resistant
Campylobacter jejuni.
Antimicrobial Agents and Chemotherapy,
(1995), 39, XI, pp.2019 -2022.
18. Charvalos E., V. Houlakis, and Y. Tselentis. Probe identification
of Campylobacter species in the Mediterranean island of Crete
and susceptibility profile to ciprofloxacin. Μedit. J. of Infect. and
Parasit. Dis. Issue October - December. (1995) Vol. X, No 4, p.
200-202.
19. Charvalos E., S. Manetas, A. Georgiladakis and J. Tselentis.
Epidemiological investigation of toxoplasmosis in rural areas in
Greece.
Mediterranean
Journal
of
Infectious
and
Parasitic
Diseases. October - December (1995) Vol. X, No 4, p. 177 -199.
20. Manetas S., J. Tselentis, and E. Charvalos.
Epidemiological
investigation of human brucellosis in Greece. Italian Journal of
Clinical Pathology. (1989), Vol. IV, N. 6 December p. 393 -396.
Greek publications and production of educational material

In Acta micribiologica Hellenica the official journal of the
Hellenic Microbiology Association more than five scientific
articles and reviews; in other Greek journals pub lications
of peer reviewed articles.

Short manual (notes) for the students (Molecular biology medical microbiology –research methodology).

Video of the laboratory course on Molecular Biology
BOOKS
1. Diagnostic
Molecular
Microbiology ,
Paschalides
Medical
Editions, Athens (1998).
2. Synospsis of cellular biology: an introduction to molecular
biology. Paschalides Medical Editions, Athens, (2002), ISBN:
960-399-092-2.
3. Clinical Microbiology protocols . Paschalides Medical editions,
(2007) ISBN: 978 -960-399-542-5.
CONGRESS ABSTRACTS
(most relevant, from about 95)
1. E. Charvalos, A. Vassilakopoulou, G. Daikos, M. Passiotou, I. Dakic,
G. Petrikkos. Charvalos Ekatherina, Integrons and Multidrug Resistance
(MDR) in Enterobacteriaceae from Food Producing Poultry.
ASM 107
General Meeting, 24/26 May 2007, Toronto, 07 -GM-A-2436-ASM
Canada.
2. Dakic I, Svabic M, Kalkani E, Charvalos E. "Pathogenic antibiotic resistant bacteria in the poultry husbandry environment in Greece:
Investigation of the phenomenon. Are there methods to control this
issue?" The second Jordanian, International congress in Immunology
and allergy, Amman, Jordan May 30 -2 June, 2007.
3.
Ivana
Dakic,
Dinos
Lilakos,
Milena
Svabic -Vlahovic,
Sofia
Christofilopoulou, Nikos Poggas and Ekatherina Charvalos. Integron
and Tn21 associated antibiotic resistance in Enterobacteriaceae from
community and hospital urine tract infection (UTI), Current status αnd
future Scenarios. Caire, 11 -16 December 2006.
4. Alexopoulos A, Voidarou C, Stefanidis C, Papadopoulos I, Vavias S,
Kalkani E, Charvalos E and E Bezirtzoglou. Antibiotic resistance
profiles and integrons in Enterobacteriaceae from the riverside Evros Ardas with respect to chemical pollution (extended abstract) Microb
Ecol. In Health and Dis. (2006) vol 18: 170 -176.
5. Fanariotis D., Svabic Vlahovic M., Antonopoulou S., Vassilopoulos
J., Kalkani E., Kouroussis A., Cernescu C. , Charvalos E. , Bebear C. .
The European network for genital mycomplasmas Prevalence of genital
mycoplasmas and their susceptibilities in three Balkan countries.
Sexually transmitted infections congress, Mykonos 7 -9 October 04.
6. Aexandra Vassilakopoulou, G. L. Daikos, L. Tzouvele kis, C.
Kosmidis, E. Charvalos, A. Avlamis, G. Petrikkos. Prevalence of Class
1
Integrons
in
Enterobacteriaceae
from
hospitalized Patients. ISDA, USA, October 04.
hospitalized
and
Non -
7. Ηatjisoteriou M., Charvalou K., E Toufeksi, A Lagadas, G Sarikakis,
G.
Petrikkos.
Prevalence
of
genital
mycoplasmas
and
their
susceptibilities to antimicrobial agents. EEC, 5th European congress of
chemotherapy and infection 17 -20 October 2003 Rhodes.
8. Vallianou N, Fourtziala E, Togas D, Miltiadous M, Gourni A,
Charvalos E and G. Petrikkos Epidemiological study of antibiotic
resistant Staphylococcus aureus strains in a general hospital of Cyprus.
1st pancyprian congrees of chemotherapy and infectious diseases 31/3
/2000, Nicosia, Cyprus
9. Charvalos, E., M.N. Tzatzarak is, F. Van Bambeke, P.M. Tulkens,
A.M. Tsatsakis, M -P Mingeot-Leclercq In-vitro antifungal activity,
cytotoxicity
and
cellular
accumulation
of
new
water -soluble
amphotericin B (AMB) – polyvinylpyrrolidone (PVP) formulations.
ESCMID, Prague May 2-5 2004.
10.
Charvalos
E.,
Charis
Liapi,
Aristeidis
Tsatsakis,
Manolis
Tzatzarakis, Panagiota Galanopoulou, Charis Koutis. New nystatin
polymeric complexes and their in vitro antifungal evaluation in a model
study with F. oxysporum. XIV World congress of Pharmacolo gy. July 712. (2002) Moscone convention centre, San Franscisco, CA.
11. Charvalos E., Tzatzarakis M., Tsatsakis A., & M. I. Shtilman.
Antifungal activities of novel controlled release water -soluble polymeric
forms of sorbic acid. ICAAC Toronto, September 17-20, 2000.
12.
Charvalos
E.,
Tsatsakis
A.,
Tzatzarakis
M.,
Kanta
A.,
Georgopoulos I, Petrikkos G. Antifungal activities of novel controlled
release water soluble polymeric forms of sorbic acid. 6th World Hellenic
Biomedical Congress, Athens 12 -14 October 2000.
13. Charvalos, E. & A. Karoutis. Antimicrobial Activity of Arf Excimer
Laser on Gram negative Bacteria. ASM General Meeting.Chigago, May
28-June 2, 1999.
14. Irini Galani, E. Charvalos & H. Giamarellou. Mechanisms of
quinolone resistance in cli nical strains of Acinetobacter baumannii .
EEC, Hamburg, 11 May 1998.
15. Achilleas Gikas, Ioanna Spyridaki, Diamantis Kofterides, Aikaterini
Charvalos, Αnna Psaroulaki & J. Tselentis. Isolation and genotypic
identification of Coxiella burnetii in 158 cases of Q fever. 1st
International Meeting of Chemotherapy, of the European Society of
Chemotherapy, Athens 21 -24 May 1996.