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Echocardiography 89 ECHOCARDIOGRAPHY (CARDIAC ULTRASOUND) Echocardiography is a technique used to evaluate the heart's activity with ultrasounds. PHYSICAL PRINCIPLE Ultrasounds represent mechanical vibrations of an elastic medium with a frequency above 20000 Hz. These waves can be represented graphically as sinusoidal waveforms characterized by: amplitude, length of wave and frequency. Figure no. 98. Sinusoidal waveform (where: T - time (period) =1/F, A amplitude, λ – length of the wave). In a homogeneous and elastic medium, ultrasounds propagate as a wave-front. When this front will meet a surface of separations between two mediums with different density, it will suffer two important physical processes: reflection (the wave will returns in the first medium) and refraction (the wave will pass in the second medium). Both physical processes are important in echography because the reflected wave will be transformed in image on the screen and the refracted wave will pass into the depth of the tissues and will offer us information about deeper structures. Figure no. 99. Physical principles of wave propagation. THE ECHOCARDIOGRAPH This device has three main components: transducer (probe), amplification system and recording system (or visualization system). 90 Physiology laboratory exercises 1. The transducer (probe) – is one of the most important parts of the device as this is the place were are ultrasounds are formed (produced) and recorded. This is possible due to the piezoelectric effect. The piezoelectric effect the piezoelectric effect is closely related to the occurrence of electric dipole moments in solids. As a result of the application of mechanical pressure an electric field will appear in certain non-conducting crystals. Pressure polarizes some crystals, such as quartz, by slightly separating the centers of positive and negative charge. The resultant electric field is detectable as a voltage. The converse effect also occurs: an applied electric field produces mechanical deformation in the crystal. Using this effect, a high-frequency alternating electric current can be converted to an ultrasonic wave of the same frequency, while a mechanical vibration, such as sound, can be converted into a corresponding electrical signal. Figure no. 100. The piezoelectric effect. In cardiology transducers with a specific frequency are used:2.5- 3.5 MHz. In time, many types of transducers were developed: ► monocrystal probe – is used today only in one-dimensional echocardiography ► mechanical probe – there is only one crystal that is moved along of an arc of circle with 60-90 degrees or there are 4 crystals in an rotating system which will send waves only when they arrive in a specific place (slit) placed on the surface of the transducer ► linear probe – it is formed by 15-30 crystals placed in linear fashion and activated together, the final image being the resultant of the component images ► electronic probe – there are many linear crystals but with a compound activation (phased array) 60-90o A B C D Figure no. 101. Schematic presentation of different types of ehocardiographic probes: A - monocrystal probe, B - mechanical probe with one crystal, C - mechanical probe with 4 crystals, D - linear probe. Echocardiography 91 2. Display (recording or visualization system) – the final image is presented on a screen with high resolution .There are 3 modalities to present the ecographical image on the screen: ►A mode (A = amplitude): the resultant image is formed by vertical deflections (spikes) according to the intensity of the echo ► B mode (B = brightness): the image is composed of points with different luminosity in correlation with the intensity of the echo. The image is presented in shades of white - grey – black. This modality can present the heart bi-dimensionally or tridimensionally. If the frequency of the frame on the screen will be over 15 frames/second we can observe the heart "in real-time". ► M mode (M = motion): the signal is obtained according to time. We have two different coordinates: amplitude (vertical) and time (horizontal). Figure no. 102. Normal anatomy of the heart (in the left), as perceived in Bmode (middle) and M-mode (right). EXAMINATION METHOD In order to avoid the bone (which reflects the signal) and the air from lungs (which can disperse the beam of waves too much) we use specific positioning of the transducer on the thorax. These positions on the thoracic surface are called echographic windows. Between the probe and the skin we use echographic gel. Standard sections used in echocardiography are (see also Figure no. 103 and Figure no. 104): ► longitudinal view: the probe is placed in the 2nd intercostal space, near the sternum (parasternal position) ► four chambers view: the probe is placed in the 5th intercostal space, on the medium clavicle line (apical position) ► short axis view: the probe is placed just below the sternum 92 Physiology laboratory exercises Figure no. 103. Main echographic windows (parasternal, apical, below the sternum and suprasternal). Figure no. 104. Main views used in transthoracic echocardiography. ECHOGRAPHY OF THE MITRAL VALVE The mitral valve (also known as the bicuspid valve or left atrioventricular valve) is a dual-flap valve in the heart that lies between the left atrium and the left ventricle. The mitral valve is connected through chordae tendineae to the papillary muscles. The examination is made with the probe placed in the parasternal position, 1-2 cm from the left border of the sternum (mitral section). The aspect in M mode is presented in Figure no. 105. ► during diastole – the mitral valve presents movements "in mirror": the anterior cusp realizes the aspect of "M" whereas the posterior cusp creates a "W" ► during systole – both cusps are represented as a single echo (line) because they are cleaved Quantitative echocardiography allows calculating parameters of the mitral valve (see Figure no. 106 and Table no. 6). Echocardiography 93 PAVD ECG SIV E A D F VMA C VMP PPVS Figure no. 105. Schematic representation of M-ECO of the mitral valve. PAVD = anterior wall of the right ventricle, SIV = interventricular septum, VMA = anterior cusp of the mitral valve, VMP = posterior cusp of the mitral valve, PPVS = posterior wall of the left ventricle, D = the opening of the mitral valve, E = maximal opening of the anterior cusp, F = protodiastolic closing of the anterior cusp, A = atrial systole, C = closing of the mitral valve. Table no. 6. Echocardiographic parameters of the mitral valve. Parameter Notation Maximum amplitude of D-E ( mm) leaflets opening Value 20 - 30 mm E-F slope (protodiastolic closing) A-C slope ( systolic closing) E-F ( mm/s) 50 - 150 mm A-C ( mm/sec ) 140-370 mm A-C (echo) - P-R (ECG) interval (A-C) - (P-R) <0.06 sec. (sec) Significance Related to the mobility of the valve and the output through the valve. Decreases in mitral stenosis Related to the enddiastolic pressure from left ventricle. Related to the enddiastolic pressure from left ventricle. Figure no. 106. Method to calculate echocardiographic parameters of the anterior cusp of the mitral valve (VMA). The opening of the VMA is the distance measured between D and E. The speed of protodiastolic closing is the tangent of the angle created by EF segment with the horizontal line. 94 Physiology laboratory exercises ECHOGRAPHY OF THE AORTIC VALVE The aortic valve has three cusps: the left coronary cusp and the right coronary cusp are placed in an anterior plane whereas the non-coronary cusp is situated in a posterior plane. The examination is performed using a transducer placed in parasternal position, with a posterior-medial orientation towards the right clavicle of the patient. VD Ao-perete ant. VAo Ao-perete post. AS Figure no. 107. Schematic representation of M-ECO of the aortic valve. VD = right ventricle, Ao = aorta, VAo = aortic valve, AS = left atrium. The aorta (see also Figure no. 108) is easily recognized as two parallel signals (anterior and posterior wall) with a sinusoidal movement (anterior during systole and posterior in diastole). Between the aortic walls we can observe the aortic valve (during systole two different parallel lines and a single signal during diastole). Ao DAo EIA Figure no. 108. Calculation of echocardiographic parameters of the aorta and the aortic valve. Ao = diameter of the aorta, DAo = opening of the aortic valve, EIA = systolic movement of the aortic root. Also we can use quantitative echography to calculate specific parameters (see Table no. 7). Table no. 7. Echocardiographic parameters of the aortic valve. Parameter Systolic opening of the aortic valve Diameter of the aortic root The systolic motion of aortic root Diameter of the left atrium Ratio of LA and DAo Notation DAo (mm) Value 17 - 25 mm Significance Decreases in aortic stenosis. Ao (mm) 20 - 37 mm EIA or Ao during S LA (mm) 5 - 15 mm Increases with age and in aortic aneurism. Related with systolic volume. LA/DAo 0.07 - 1.1 23 - 44 mm Related with the end-diastolic pressure of the left ventricle. Increased in valvular diseases. Echocardiography 95 ECHOGRAPHY OF THE LEFT VENTRICLE For the examination the probe has to placed in the parasternal standard position, with an inferior and lateral orientation. The aspect of the left ventricle in M mode is presented in Figure no. 109. Left ventricle appears as a free space between interventricular septum (anterior) and the posterior wall of the left ventricle (PWLV). The anterior and posterior walls move closer to each other in systole and aloof in diastole. VD SIV DTDVS VS DTSVS PPVS Figure no. 109. Schematic representation of M-ECO of the medioventricular section. VD = right ventricle, SIV = interventricular septum, VS = left ventricle, PPVS = posterior wall of the left ventricle. The method to calculate quantitative parameters of the left ventricle is also shown: DTDVS = enddiastolic diameter of the left ventricle, DTSVS = end-systolic diameter of the left ventricle. The thickness of the wall is always measured during diastole. Table no. 8. Echocardiographic parameters of the left ventricle. Parameter Thickness of the interventricular septum Thickness of the posterior wall of the left End-diastolic diameter of the left ventricle (EDD) End-systolic diameter of the left ventricle (ESD) Value 6 - 11 mm Significance Increases in ventricular hypertrophy. 6 - 11 mm Increases in ventricular hypertrophy. 24 - 40 mm Increases in ventricular dilatation. 35 - 57 mm Analyses ventricular performance. Quantitative echocardiography permits us to calculate diameters of the ventricle and the ventricular wall thickness. Using geometric models we can approximate the volumes of left ventricle during cardiac cycle (Figure no. 110 and Table no. 9). Figure no. 110. Geometric model of the left ventricle (ellipsoid with axial diameter twice the cross-sectional diameter). 96 Physiology laboratory exercises Table no. 9. Performance parameters of the left ventricle calculated using M mode. Parameter Notation Left ventricular Sf shortening fraction Mean velocity of mVcf shortening of the circumferential fibers Ejection fraction Ef Stroke volume SV Cardiac output CO Formula ( EDD ESD) x 100 EDD EDD EDS ejection time x EDD SV x 100 EDD 3 ESD 3 EDD 3 SV x HR Value 28 - 41 % 0.85 - 1.4 circ/sec 50 - 80 % 70 - 80 ml 5-6l ECHOGRAPHY OF THE RIGHT VENTRICLE We examine the right ventricle in the same standard window as the left ventricle. Dimensions of the right ventricle are calculated in B mode using the four chambers view (Figure no. 111). VS AS VD AD Figure no. 111. Echocardiographic aspects as recorded using the four chambers view. VS = left ventricle; VD = right ventricle; AS = left atrium; AD = right atrium. ECHOGRAPHY OF THE RIGHT AND LEFT ATRIUM: The left atrium is examined in aortic standard view. We can notice the left atrium as a free space posterior from the aorta. A foreign signal detected within this compartment can mean an atrial tumor or an atrial thrombus. It is difficult to examine the right atrium in M mode. Because of its retrosternal position, we examine this structure in B mode and four chambers view.