Download Gpositive cocci

1. A Gram positive cocci that is catalase positive was plated on the mannitol salt
plate in area c. Name that organism.
(mansalt)
a. Streptococcus durans
b. Streptococcus pyogenes
c. Staphylococcus aureus
d. Staphylococcus epidermidis
2. Observe the Mannitol salt plate. How are mannitol fermenting organisms
detected?
(mansalt)
a. Observation of a yellow color surrounding cell growth.
b. Observation of a pink color surrounding cell growth.
c. Observation of cell growth.
d. Zones of clearing around the colonies.
3. Observe the plate. The organism is growing on a Blood plate with a Taxo P disc.
By looking at the plate you can conclude that:
this organism is not sensitive to optichin.
What observation allows you to make this conclusion?
(blood3)
a. There is no zone of growth inhibition around the Taxo P disc
b. There is a zone of growth inhibition around the Taxo P disc
c. The color of the media around the cells is green.
d. The bacteria are able to grow on the Blood plates.
4. Coagulase and hemolysins are example of ________________ that are
characteristic of organisms that cause disease.
a. toxins
b. virulence factors
c. transcription factors
d. repressors
5. An encapsulated organism is a gram positive cocci that is catalase negative, alpha
hemolytic and solubilizes bile. Name the organism.
a. Staphylococcus aureus
b. Bacillus subtilus
c. Pseudomonas aeruginosa
d. Streptococcus pneumoniae
6. The coagulase test is used to differentiate Staphylococcus aureus and
Staphylococcus epidermidis . Name the genus and species of the organism that
produced the result on the right.
(coagulase)
a. Staphylococcus aureus
b. Staphylococcus epidermidis
7. The inulin fermentation test is used to differentiate alpha hemolyitic
Streptococcal species. Based on the results of the inulin fermentation test, choose
the genus and species of the organism growing in tube 1.
(inulin)
a. Streptococcus pneumoniae
b. Streptococcus lactis
c. Streptococcus durans
d. Streptococcus pyogenes
8. Observe the photo. The photo shows Gram positive cocci growing on a mannitol
salt plate. How would the organism that is streaked in area B, test in a coagulase
test?
(mansalt)
a. there would be no clotting of the plasma.
b. There would be clotting of the plasma.
9. A simple slide test for a cellular enzyme will allow differentiation of
Staphylococci from Streptococci. Name the enzyme.
a. oxidase
b. Beta galactosidase
c. coagulase
d. gelatinase
e. catalase
10. Staphylococcus aureus and Staphylococcus epidermidis can be differentiated by
a. ability to ferment mannitol
b. sensitivity to Taxo A
c. presence of catalase.
d. ability to degrade starch.
11. What is the type of hemolysis is displayed on the blood plate? The zone around
the colonies is clear.
(hemolysis)
a. Beta hemolysis
b. Alpha hemolysis
c. Gamma hemolysis
d. No hemolysis apparent on the plates.
12. Which will not differentiate Streptococcus pneumoniae from Streptococcus
lactis ?
a. hemolysis pattern
b. bile solubility test
c. sensitivity to optichin
d. fermentation of inulin
13. Streptococcus _________________ is taxo A sensitive.
a. durans
b. pyogenes
c. coli
d. subtilis
14. What types of cells are lysed in a hemolysis assay?
a. bacterial cells
b. white blood cells
c. plant cells
d. red blood cells
15. Mannitol salt plates are selective. What types of organisms are selected for on
mannitol salt plates?
a. mannitol fermenting.
b. lactose fermenting.
c. salt tolerant organisms
d. nitrogen fixing organisms
16. Streptococcus pneumoniae has autolytic enzymes. Addition of ______to a culture
of Streptococcus pneumoniae will activate these enzymes and results in cell lysis
and clearing of the broth.
a. bile and other detergents.
b. antibody
c. antibiotics
d. iodine
17. A positive inulin test results from ____________ of inulin.
a. hydrolysis
b. lysis
c. fermentation
d. production
18. This is a picture of a mannitol salt plate... in which section (a, b, or c) is an
organism that ferments mannitol?
(mansalt)
a. (a)
b. (a) and (b)
c. (b)
d. (a) and (c)
e. all of them can ferment mannitol
19. What can be interpreted from a positive bile solubility test?
a. The organism is salt tolerant.
b. The organism is capable of inulin fermentation.
c. The organism contains autolytic enzymes.
d. The organism is capable of forming a fibrin clot.
20. Staphylococcus aureus and Staphylococcus epidermidis can be differentiated by
a.
b.
c.
d.
sensitivity to Taxo A
production of coagulase
ability to grow in high salt
inulin fermentation
21. A Gram positive cocci is catalase positive. Name the genus.
a. Staphylococcus
b. Streptococcus
c. Pseudomonas
d. S. aureus
22. A Gram positive cocci that is catalase positive was plated on the mannitol salt
plate in area c. Name that organism.
(mansalt)
a. Streptococcus durans
b. Streptococcus pyogenes
c. Staphylococcus aureus
d. Staphylococcus epidermidis
23. Normal or resident flora would not be characterized as
a. Parasitic
b. Mutualistic
c. Commensal
d. none of the above