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CURRICULUM PLAN 2015-16 (Department of Botany, Kalindi College) Course B.Sc.(P) Physical Sciences Semester – IV Paper : Biology II Name of the Teacher: Dr. Kalpana Kumari Topic References Approximate schedule THEORY Unit Techniques in Biology •Principles of microscopy Karp, G. (2010). Cell Biology, John Wiley & Sons, U.S.A. 6th edition. January February and •Light Microscopy Phase contrast microscopy •Fluorescence microscopy;Sheeler, P and Bianchi, D.E. (2006) Cell and Molecular Biology, 3rd Confocal microscopy •Sample Preparation for lightedition, John Wiley & sons NY microscopy; Electron microscopy (EM)- Scanning EM and Scanning Transmission EM (STEM); •Sample Preparation for electron microscopy; X-ray diffraction analysis Unit Cell as a unit of Life •The Cell Theory and Prokaryotic Cell •Comparison Between Eukaryotic cells and Prokaryotic Cell •Cell size and shape •Eukaryotic Cell components Raven, P.H et al (2006) Biology7th edition Tata McGraw Hill Publications, New Delhi March and April Campbell, N.A. and Reece, J. B. (2008) Biology 8th edition, Pearson Benjamin Cummings,. San Francisco •Class Test •Assignment/Presentation PRACTICAL G1 Topic 1.To study prokaryotic cells (bacteria), viruses, eukaryotic cells with the help of light and electron micrographs. Karp, G. (2010). Cell Biology, John Wiley & Sons, U.S.A. 6th edition. January 2. Study of the photomicrographs Karp, G. (2010). Cell Biology, John of cell organelles Wiley & Sons, U.S.A. 6th edition. 3. To study the structure of plant cell through temporary mounts. 4. To study the structure of animal cells by temporary mounts-squamous epithelial cell and nerve cell. 5. Preparation of temporary mounts of striated muscle fiber. Karp, G. (2010). Cell Biology, John 6. To prepare temporary stained preparation of Wiley & Sons, U.S.A. 6th edition. mitochondria from striated muscle cells /cheek epithelial cells using vital stain Janus green 7. To prepare temporary stained squash from root tips of Allium cepa and to study the various stages of mitosis. 8. Study the effect of temperature, organic solvent on semi permeable membrane 9. Demonstration of dialysis of starch and simple sugar. 10. Study of plasmolysis and deplasmolysis on Rhoeo leaf. 11. Measure the cell size (either length or breadth/diameter) by micrometry. 12. Study the structure of nuclear Karp, G. (2010). Cell Biology, John pore complex by photograph Wiley & Sons, U.S.A. 6th edition. (from Gerald Karp) Revision of Practical January February February March March March April April Suggested Readings 1.Karp, G. (2010). Cell Biology, John Wiley & Sons, U.S.A. 6th edition. 2.Sheeler, P and Bianchi, D.E. (2006) Cell and Molecular Biology, 3rd edition, John Wiley & sons NY 3.Raven, P.H et al (2006) Biology7th edition Tata McGraw Hill Publications, New Delhi