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Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
Altogen Biosystems offers the HeLa Transfection Reagent
among a host of 100+ cell line specific In Vitro Transfection
Kits.
The HeLa Transfection Reagent is an liposome formulation
reagent, and it has been developed to provide high transfection
efficiency with the HeLa cell line.
This cell line is a good host for a wide variety of research in cell
and molecular biology. When cultured in vitro, HeLa cells are a
monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression
studies, molecular and cell biology research applications.
Purchase HeLa Transfection Kit at www.Altogen.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
HeLa Cell Line Description and Applications
HeLa cells
The HeLa cell line originates from the cervical cancer cells of
Henrietta Lacks, a 31-year old female patient, in 1951. The
HeLa cell line is remarkable in that it is the first human cell line
able to survive in vitro indefinitely. The cells were taken from a
tissue sample from the adenocarcinoma and HeLa cells have a
modal chromosome number of 82. HeLa cells have an active
type of telomerase during cell division and as a result the cell
line proliferates extremely quickly in comparison to other cell
lines. Jonas Salk used HeLa cells to test the polio vaccine and
HeLa cells have been an invaluable tool in a huge array of
research from cancer to gene mapping to AIDS and studying
the toxic effects of radiation. The HeLa cell line contains human
papilloma virus sequences and tests positive for lyso-PC and
keratin.
Atcc.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
HeLa Transfection Protocol
1. Plate 10,000 - 15,000 HeLa cells per well in 0.5 ml of complete growth medium 12–24 hours prior to
transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent,
and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex,
therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with HeLa cells (from step
2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of
Transfection Enhancer reagent 12-24 hours after transfection
If the viability of HeLa cells being transfected is affected at 16 - 24 hours post-transfection, the level of
cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to
transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
HeLa Transfection Kit Product Details
•
Proprietary cationic lipids formulation
•
Transfection protocols provided for transfection of proteins, DNA, mRNA, siRNA, shRNA and
microRNA
•
Transfection Enhancer reagent provided with the kit
•
Produce higher level of recombinant protein expression with minimal disruption of normal cell
function
•
Generate physiologically relevant data you can trust
•
Effective for plasmid DNA/siRNA co-transfection
•
Easy-to-use transfection protocol with reproducible results
•
Low cytotoxicity
Developed and manufactured by Altogen Biosystems (www.Altogen.com)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
Data
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in HeLa cells transfected by
Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol.
Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were
used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented
as means ± SD (n=3).
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
Data
Figure 2. Protein expression of Cyclophilin B in HeLa cells. DNA plasmid expressing Cyclophilin B or siRNA
targeting Cyclophilin B were transfected into HeLa cells following Altogen Biosystems transfection protocol. At
72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by
total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells)
HeLa Transfection Kit Benefits
• Pre-optimized transfection protocol for HeLa cell line
• Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
• Free of serum and protein of animal origin
• Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
• Easy to use HeLa transfection protocol ensures great performance with expedited experimental timeline
• Equally efficient for single or multiple transfections
• Can be used for transient transfection and development of stable HeLa cell lines
• Bio-degradable after endocytosis
• Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]