Download For each question, choose the ONE BEST answer or completion

Molecular biology 25% , 2.5 points each
For each question, choose the ONE BEST answer or completion.
1. Which of the following steps is NOT part of translation?
A. Synthesis of mRNA
B. Charging of tRNA
C. Initiation of protein synthesis
D. Elongation of the polypeptide chain
E. Termination of protein synthesis
2. The enzyme that attaches the amino acid to its tRNA is
A. aminoacyl mRNA synthase.
B. aminoacyl tRNA synthase.
C. amino acid attachment enzyme.
D. elongation factor tu.
E. tRNA polymerase.
3. Bacterial mRNAs usually have the nucleotide sequence
5_ GGAGG 3_, which bonds to
A. tRNA.
B. methionine.
C. the 30S ribosomal subunit.
D. the 40S ribosomal subunit.
E. protein initiation factors.
4. The enzyme that catalyzes the formation of a peptide bond between
two amino acids is
A. amino acid transferase.
B. peptide joinase.
C. peptidyl transferase.
D. peptide elongation factor.
E. puromycin.
5. Which of the following do NOT play a part in the termination of
protein synthesis?
A. The codons AUG, AGG, and GUA
B. The codons UAA, UAG and UGA
C. Release factor 1
D. Release factor 3
E. Peptidyl transferase
6. The base hypoxanthine in the nucleotide inosine can hydrogen bond
with
A. G, U, and C.
B. G, U, and A.
C. G, A, and C.
D. U, C, and A.
E. A only.
7. A cDNA clone library is a collection of clones representing
A. the entire genome of the sample tissue.
B. all protein-coding gene sequences in the genome of the sample tissue.
C. all intronic sequences of genes expressed in the sample tissue.
D. the mRNAs expressed in the sample tissue.
E. all tRNAs being used in the sample tissue.
8. To sequence DNA using the automated dideoxy chain termination
method, the reaction mixture should contain the DNA to be sequenced
plus
A. the four deoxynucleotides, dATP, dCTP, dGTP, and dTTP.
B. the four dideoxynucleotides, ddATP, ddCTP, ddGTP, and ddTTP, each
carrying a different fluorescent tag.
C. a DNA primer.
D. DNA polymerase.
E. all of the above.
9. The technique called
A. Southern blotting is used to analyze RNA.
B. western blotting is used to analyze DNA.
C. eastern blotting is used to analyze RNA.
D. Southern blotting is used to analyze DNA.
E. northern blotting is used to analyze protein.
10. Each cycle in the polymerase chain reaction involves the following
steps:
A. Polymerization then annealing then denaturation
B. Denaturation then polymerization then annealing
C. Denaturation then annealing then polymerization
D. Denaturation then chimera formation then polymerization
E. Denaturation then polymerization then sequencing