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OIE Reference Laboratory Reports Activities in 2011 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Address of laboratory: White spot disease Aquaculture Pathology Laboratory Department of Veterinary Science and Microbiology University of Arizona Building 90, Room 202 Pharmacy/Microbiology Tucson, AZ 85721 UNITED STATES OF AMERICA Tel.: (1.520) 621.84.14 Fax: (1.520) 621.48.99 e-mail address: website: [email protected] http://microvet.arizona.edu/research/aquapath/index.htm Name (including Title and Position) of Head of Laboratory (Responsible Official): Dr Donald V. Lightner Professor Name(including Title and Position) of OIE Reference Expert: Dr Donald V. Lightner Professor Name (including Title and Position) of writer of this report (if different from above): Annual reports of OIE Reference Centres, 2011 Dr Donald V. Lightner & Linda Nunan, Assistant Staff Scientist 1 White spot disease Part I: Summary of general activities related to the disease 1. 2. Test(s) in use/or available for the specified disease/topic at your laboratory Test For Specificity Total PCR Surveillance & SPF verification All known WSD viral genotypes 1294 Histology Initial diagnosis & surveillance Lesions 2385 ISH Confirmatory diagnosis All known WSD viral genotypes 0 Real-time PCR Confirmatory diagnosis & quantification of viral load All known WSD viral genotypes 68 Production and distribution of diagnostic reagents Date Country Material provided Quantity sent 02/23/2011 USA WSSV infected tissue 1vial 03/29/2011 Saudi Arabia WSSV PCR kit 1 Kit 05/26/2011 Mexico WSSV infected tissue 1vial 05/31/2011 Argentina WSSV infected tissue 1vial 06/01/2011 Saudi Arabia WSSV infected tissue 1vial 06/11/2011 India WSSV infected tissue 1vial 07/14/2011 Malaysia WSSV infected tissue 1vial 09/29/2011 Vietnam WSSV infected tissue 1vial 10/06/2011 Singapore WSSV plasmid DNA 1 vial 11/23/2011 Saudi Arabia WSSV infected tissue 1vial Part II: Activities specifically related to the mandate of OIE Reference Laboratories 3. 2 International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results Annual reports of OIE Reference Centres, 2011 White spot disease Ring trials: White spot syndrome virus detection by PCR Country Month & Year No. Labs participating Correct results Ecuador August 2011 1 YES Madagascar February 2011 1 YES August 2011 1 YES Saudi Arabia February 2011 1 YES Mexico February 2011 7 Lab 1: YES Lab 2: YES Lab 3: YES Lab 4: YES Lab 5: YES Lab 6: YES Lab 7: YES August 2011 5 Lab 1: YES Lab 2: YES Lab 3: YES Lab 4: YES Lab 5: YES Panama February 2011 1 YES India August 2011 1 YES Vietnam February 2011 2 Lab 1: YES Lab 2: YES August 2011 1 YES N-An = not analyzed 4. Preparation and supply of international reference standards for diagnostic tests or vaccines See section Part I-2 .5. White spot syndrome virus infected shrimp tissue Plasmid DNA for use as positive control in PCR reactions White spot syndrome virus kits Research and development of new procedures for diagnosis and control UAZ developed and published an improved, one-step PCR method for detection of WSSV, which is based on the OIE recommended method published by LO et al. (1996). Nunan, L.M. and D.V. Lightner. 2011. Optimized PCR assay for detection of white spot syndrome virus (WSSV). Journal of Virological Methods 171: 318-321. 6. Collection, analysis and dissemination of epizootiological data relevant to international disease control None Annual reports of OIE Reference Centres, 2011 3 White spot disease 7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned 8. Quality assurance of sample material provided is achieved by pre-testing the sample material for the pathogen of interest, by PCR, prior to shipment. Biosafety and biosecurity is achieved by following federal regulations for disposal of infectious materials and through the inland location of the laboratory, as no marine pathogens would be able to survive in the natural environment of the Sonora Desert. Provision of consultant expertise to OIE or to OIE Member Countries Chapters for the OIE Aquatic Animal Health Code – assisted in preparation Lightner DV. 2011. Aquatic Animal Health Code, 14th Edition. World Organization for Animal Health (Office International des Epizooties = OIE), Paris: - Infectious hypodermal and haematopoietic necrosis, Chapter 9.2. - Infectious myonecrosis, Chapter 9.3. - Necrotizing Hepatopancreatitis, Chapter 9.4. - Taura syndrome, Chapter 9.5. - White spot disease, Chapter 9.6. - White tail disease, Chapter 9.7. - Yellow head disease, Chapter 9.8. Chapters for the OIE Manual of Diagnostic Tests for Aquatic Animals Lightner DV. In press (2012). Diagnostic Manual for Aquatic Animal Diseases, 7th Edition. World Organization for Animal Health (Office International des Epizooties = OIE), Paris: - Disinfection of Crustacean Farms, Section C of Methods for Disinfection of Aquaculture Establishments. - Diseases of Crustaceans, General Information. - Infectious Hypodermal and Hematopoietic Necrosis. - Infectious Myonecrosis. - Taura Syndrome. - Tetrahedral Baculoviruosis (Baculovirus penaei). - Spherical Baculovirosis (Penaeus monodon-type baculovirus). Advisor to the Aquatic Animal Health Standards Commission, Office International Des Epizooties, Paris. Term 2009-2011, Advisor (~10 days/year). I was appointed to a third 3-year term by the OIE Central Bureau, beginning in May 2009, on the OIE Aquatic Animal Health Standards Commission (AAHSC) which meets 2 times per year to consider new diagnostic methods for OIE listed and notifiable aquatic animal diseases; to consider changes to the OIE Aquatic Animal Code; and to make recommendations to the Director General and to the OIE World Assembly of Member Country Delegates. In 2011, I participated in the AAHSC meeting of October 3-7 at the OIE Headquarters in Paris. 9. Provision of scientific and technical training to personnel from other OIE Member Countries • Hosted Borlaug scholar Mr Bambang Hanggono, of the Indonesian Government’s Situbondo Laboratory. Mr Hanggono visited the Aquaculture Pathology Laboratory for 12 weeks (17 September to 3 December, 2011). Mr Hanggono’s training emphasized histopathology and molecular diagnostic tests for major shrimp diseases including white spot syndrome virus. 10. Provision of diagnostic testing facilities to other OIE Member Countries We received “official” samples for confirmation of WSSV infection from the Kingdom of Saudi Arabia and Mozambique. 4 Annual reports of OIE Reference Centres, 2011 White spot disease 11. Organisation of international scientific meetings on behalf of OIE or other international bodies None 12. Participation in international scientific collaborative studies • Development of qPCR kits with Life Technologies of Singapore & USA. Provided samples of frozen shrimp tissue infected with IMNV and genome sequence information to Life Technologies (formerly Applied Biosystems) of Singapore & USA for development of qPCR/RT-PCR test kits. In late 2011, we received a real time thermocycler for use in validating shrimp virus kits for Life Technologies. 13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) Presentations at international conferences and meetings Invited speaker OECD Symposium, Halifax, Canada. Given by OECD Co-operative Research Program, Paris, 8/7/2011. Invited keynote speaker, Honduras. Given by V Simposio Nicovita, Honduras, 11/24/2011. (100%). Invited keynote speaker, Ecuador. Given by XIII Congreso Ecuatoriano de Acuicultura and Aquaexpo, 10/20/2011. Invited speaker - OIE Global Conference on Aqiuatic Animal Health, Panama. Given by OIE , 6/29/2011. Invited keynote speaker, Panama City, Panama. Given by El Comite Organizador del X Simposio International de Camaron de Cultivo y Exhibicion Comercial, 5/13/2011. Scientific publications in peer-reviewed journals Lightner D.V. and R.M. Redman. 2010. The global status of significant infectious diseases of farmed shrimp. Asian Fisheries Society Journal 23(4): 383-426. Lightner D.V. 2011. Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): a review. Journal of Invertebrate Pathology 106: 110-130. Lightner, D.V. 2011. Status of shrimp diseases and advances in shrimp health management. pp. 121-134, in M.G. Bondad-Reantaso, J.B. Jones, F. Corsin and T. Aoki (editors), Diseases in Asian Aquaculture VII. Fish Health Section, Asian Fisheries Society, Selangor, Malaysia. 385 p. Nunan, L.M. and D.V. Lightner. 2011. Optimized PCR assay for detection of white spot syndrome virus (WSSV). Journal of Virological Methods 171: 318-321. Stentiford, G.D. and D.V. Lightner. 2011. Cases of White Spot Disease (WSD) in European shrimp farms. Aquaculture 319: 302-306. Lightner, D.V. and R.M. Redman. "In press". Development of specific pathogen-free (SPF) shrimp stocks and their application to sustainable shrimp farming. Woodhead Publishing, LTD., Cambridge. UK. Aoki, T., J.R. Bonami, D.V. Lightner, C.F. Lo, G.D. Stentiford, J.H. Leu, J. M. Vlak, K. Soderholl, P.J. Walker, T.W. Flegel, H.C. Wang, X. Xu, and H. Yang. 2012. Nimaviridae. In A.M.Q. King, M.J. Adams, E.B. Carstens and E J. Lefkowitz, editors: Virus Taxonomy. 9th Report of the International Committee on Taxonomy of Viruses, Oxford, Elsevier, pp. 229 - 234. Annual reports of OIE Reference Centres, 2011 5 White spot disease Other communications Popular Press Lightner, D.V. 2011. Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. . Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. Panorama Acuícola 16(4): 8-20. Arce, S.M., S.M. Moss and D.V. Lightner. 2011. Biosecurity principles for sustainable production using SPF shrimp. Global Aquaculture Advocate. May/June 2011, 14-16. Cuéllar-Anjel, J., R. Chamorro, B.White-Noble, P.Schofield and D.V. Lightner. 2011. Testing finds resistance to WSSV in shrimp from Panamanian breeding program. Global Aquaculture Advocate July/August 2011, 65-66. Cuéllar-Anjel, J., R. Chamorro, B.White-Noble, P.Schofield and D.V. Lightner. 2011. White shrimp Litopenaeus vannamei significantly resistant against WSSV. Panorama Acuícola 16(5): 46-48. Cuéllar-Anjel, J., , B.White-Noble, P.Schofield R. Chamorro and D.V. Lightner. 2011. Selected families of white shrimp Litopenaeus vannamei from Panama breeding program show important resistance to WSSV. November/December AQUA Culture Asia Pacific Magazine, pp. 36-37 _______________ 6 Annual reports of OIE Reference Centres, 2011