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Unit 5 Cellular Energy I. Enzymes (159) There are countless numbers of chemical reactions occurring in every cell all the time. Virtually all of these reactions enzymes catalytic require the action of _______, Speeds up proteins __________. A catalyst _________a chemical reaction without being _______________________. An enzyme Used up in the reaction works by lowering the activation energy, that is the energy required to __________________. Get the reaction going Enzymes share four common features: A. Enzymes work best within a narrow pH temperature _____ and _____________ range. This is shape because of the importance of the ______ conformation or ____________ of the enzyme. When the pH or temperature is altered, the Chemical bonds _____________ and interactions are also changed, changing the shape of the enzyme. B. Enzymes do not make a reaction happen that couldn’t happen on its own; they simply make the reaction occur much _________. faster Used up in the reaction C. Enzymes are not ________________. The same enzyme may be used over and over again. D. Enzymes are __________________. Substrate-specific The substrate is the___________. The reactant substrate fits into a portion of the enzyme called the ____________. As the Active site substrate fills the active site, this leads to a phenomenon known as __________, Induced fit almost like a _________. It is this squeeze induced fit that is responsible for lowering the ________________of the Activation energy reaction. Metabolism (218-221) All chemical reactions in a cell are metabolism referred to as cell ______________ Metabolic pathways- series of chemical reactions in which the __________of one product reaction is the ___________for the next reactant reactions. 2 Types: energy A. Catabolic pathways- Release _____________by breaking down larger molecules into smaller molecules organic EX. Cellular Respiration- ______ energy molecules broken down to release _______ for cell to use. B. Anabolic pathways- use the energy released by catabolic pathways ______________molecules from To build larger ______________ molecules. smaller Light energy ◦ EX. Photosynthesis- ______________from Sun is ______________ to Chemical ______________in converted energy form of glucose. ◦ The relationship of anabolic & catabolic pathways results in the ________________________within an Continual flow of energy organism as well as within an ecosystem. III. CHEMICAL ENERGY AND LIFE (pp. 218-221) A. Energy and Life Energy is the ___________________. Cells Ability to do work require energy for ________ metabolic reactions, _____ active transport,________, and maintaining Cell division ___________. We obtain energy from _____, homeostasis food but most energy originally comes from the sun. Plants are able to capture the sun’s energy and use it to produce glucose,________. This C6H12Ooccurs 6 process, known as___________, in the _________of plant cells. photosynthesis chloroplasts The reaction for photosynthesis is: C6H12O6 O2 CO2 Energy H2 O ______+______+__________________+_____ Plants and other organisms that can make their own food are known as autotrophs ________________. Humans and other organisms that have to obtain food heterotrophs are known as ______________. Although glucose is the preferred energy molecule lipids carbohydrates _______, for the cell, other ___________, proteins can also be broken down and _______ for energy. B. Chemical Energy The chemical energy in food (and all molecules) is stored in the_____________. chemical bonds This is__________ energy. When the bonds potential are broken and the _________ electrons are released, some energy is lost as ________, but a heat percentage of the potential energy can be converted to useable energy in the form of _____. ATP Food molecules cannot be used as a direct energy source for the cell because food contains too much energy ... if it were wasteful released all at once, it would be _______ and ____________. destructive C. ATP ___________________is Adenosine TriPhosphate a __________ nucleotide that is composed of: adenine ___________ - nitrogen base ___________ - 5 carbon sugar ribose 3 phosphate ___________________Groups Energy is stored in __________________________. These Phosphate-phosphate bonds “rechargeable batteries” are mostly present in the cell in two forms: ATP - Adenosine Triphosphate. Has 3 phosphate groups. ADP - Adenosine Diphosphate. Has 2 phosphate groups. _____ ATP has the most phosphate bonds, and potential or therefore contains the most ________ stored energy. Cells are able to use and recycle ATP by breaking off or adding a phosphate group. _________ 1. Energy can be used when a broken phosphate bond is _________. ________ _________________ ATP ADP + P 2. Energy is stored; that is, ATP is “recharged” by adding a phosphate group to ADP + P → ATP *Under normal conditions, both of these processes are occurring at the same time and all the time in a cell* http://vilenski.org/science/safari/cellstructure/chloroplasts.html REACTIONS of PHOTOSYNTHESIS Unit 4 http://www.science.siu.edu/plant-biology/PLB117/JPEGs%20CD/0076.JPG Research into PHOTOSYNTHESIS began centuries ago with this simple question: When a tiny seed grows into a huge plant, where does all the “new plant” come from? CO2 and H20 http://www.nofretete-page.de/gemischtNeu/TN_plant_grow_w.JPG THE BIG PICTURE Scientists discovered that in the sunlight plants transform CO2 and presence of _________, water into carbohydrates and release oxygen. Carbon WATER _____________ + ____________ dioxide 6 CO2 + ____________ 6 H 2O _____________ Sugars Oxygen _______________ + ____________ → C6H12O6 + ____________ 6 O2 _______________ PHOTOSYNTHESIS: Reactants & Products LIGHT ENERGY 6CO2 + 6H2O SUGAR + 6O2 In addition to water and , Carbon dioxide http://fig.cox.miami.edu/~cmallery/255/255phts/255phts.htm chlorophyll __________________ and light energy __________________ are needed for photosynthesis to happen. Chloroplast Found mainly in the _______ leaves of most plants Disk-shaped organelles contain 2 main compartments ◦ Thylakoids- flattened saclike membranes that are arranged in ____________________ stacks, this is where the light-dependent rxn take place here. thylakoids ◦ Grana- stacks of ____________ Fluid-filled spaces that is outside ◦ Stroma- __________________ grana this is where light-independent rxn the _______, takes place absorbing Pigments- light ___________colored molecules Found in thylakoid __________ membranes Different pigments absorb specific wavelengths _____________ of light absorb Chlorophyll ___________- ______most strongly in the Violet-blue ____________ region of the visible light reflect spectrum & __________ light in the green region of the spectrum. (Why leaves ______ appears green to our eyes) More abundant than other pigments in leaves, thus hides the colors of the other pigments. Different types Chlorophyll a & b called __________________ Accessory pigments- allows plant to absorb different __________ wavelengths of light _______ energy ______________absorb Carotenoids _________in Blue & green _______________region &________, reflect Yellow , orange & red __________________________ Ex. β – carotene In fall production of chlorophyll ____________________slows down or stops, thus bringing out the colors of the accessory pigments. http://son.nasa.gov/tass/images/cont_emspec2.jpg http://www.ology.amnh.org/einstein/lightmatterenergy/light.html Visible light is made up of many different wavelengths _______________of light Your eyes “see” different sizes of waves as different colors ___________ http://www.simontucket.com/_Portfolio/PortLarge/L_Il_Prism.jpg BUT… We “see” reflected light REFLECTED Light wavelengths that are ___________ off of objects bounce back to our eyes . That is the ___________ we “see” COLOR Image modified from: http://www.visibledreams.net/Web/color/color_3.html ENERGY & ELECTRONS http://www.mhhe.com/biosci/pae/botany/uno/graphics/uno01pob/vrl/images/0160.gif When chlorophyll absorbs light, the energy is transferred to electrons in the chlorophyll molecule. ELECTRONS HIGH ENERGY ____________ make photosynthesis work! http://www.wjcc.k12.va.us/ROBB/Atom%20Animation%20Resources_files/image003.gif PHOTOSYNTHESIS HAPPENS IN CHLOROPLASTS Proteins that are part of the thylakoid membrane organize ________________________________ Light absorbing PIGMENTS into clusters called _____________________ PHOTOSYSTEMS http://www.science.siu.edu/plant-biology/PLB117/JPEGs%20CD/0076.JPG LIVING THINGS NEED ENERGY CARRIERS Molecule that carries = _______ ATP Molecule that carries HIGH ENERGY ELECTRONS = ___________ NADP+ Images by Riedell Frying pan image from: BIOLOGY by Miller and Levine; Prentice Hall Publishing©2006 NADP ____________ NADP+ is one of the carriers that cells use to transport high energy electrons. + 2 e H ________ + _____ + _____ → ____________ LIGHT DEPENDENT REACTION Requires ______________ LIGHT membranes Molecules are part of THYLAKOID ________________ PHOTOSYSTEMS II & I Made up of __________________ connected by ______________________ ELECTRON TRANSPORT CHAIN & ___________________ ATP SYNTHASE Uses light energy to change ADP + P → _______ ATP NADPH NADP+ + 2e- + H + → _________ H20 molecules and Breaks apart ______ releases _____________ oxygen Pearson Education Inc; Publishing as Pearson Prentice Hall LIGHT-DEPENDENT REACTIONS See an animation about the ELECTRON TRANSPORT CHAIN SEE A MOVIE ATP SYNTHASE IN ACTION http://www.stolaf.edu/people/giannini/flashanimat/metabolism/atpsyn1.sw http://www.stolaf.edu/people/giannini/flashanimat/metabolism/photosynthesis.swf CALVIN CYCLE (also called _________________________) LIGHT INDEPENDENT ____________ DOES NOTrequire ____________ LIGHT STROMA between thylakoids Happens in _________ NADPH donates Hydrogen _______________ + electrons ENERGY ATP donates _________________ CO2 donates ______________ Carbon & oxygen to make __________ GLUCOSE http://www.estrellamountain.edu/faculty/farabee/biobk/BioBookCHEM2.html Calvin Cycle CO2 Enters the Cycle Energy Input 5-Carbon Molecules Regenerated 6-Carbon Sugar Produced Sugars and other compounds See Calvin cycle animation PHOTOSYNTHESIS OVERVIEW Pearson Education Inc; Publishing as Prentice Hall