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Table of Abbreviations
ac: anterior commissure
bmn: branchio-motor nuclei
cb: cerebellum
dt: dorsal thalamus (aka. thalamus)
dvdt: dorso-ventral diencephalic tract
e: epiphysis
fc: facial nerve
fg: facial ganglion
hci: hindbrain commissural interneurons
hg: hindbrain glia
ht: hypothalamus
LL: lateral line nerve
llf: lateral/dorsal-longitudinal fasciculus
mlf: medial-longitudinal fasciculus
n: nasal pit
nmlf: nucleus of the medio-longitudinal fasciculus
npc: nucleus of the posterior commissure
npoc: nucleus of the post-optic commissure
oc: optic chiasma
on: optic nerve
pc: posterior commissure
poc: post-optic commissure
r: rhombomere
rb: Rohon-Beard neurons
sm: stria medullaris (habenular afferents)
sma: spinal motor axons
sot: supra-optic tract
soc: supra-optic commissure
t: telencephalon
tc: optic tectum
td: trochlear decussation
tg: trigeminal ganglion
tgt: tegmentum
tptc: tract of the posterior tubercular commissure
tvc: tegmental ventral commissure
vg: vagal nerve
vt: ventral thalamus (aka. pre-thalamus)
Specification of brain territories inside the neural plate
(all pictures are oriented anterior to the left)
• Forebrain (F in red) and midbrain (M green) progenitors
bud stage
F
1 somite stage
F
M
F
M
Dorsal view
Lateral view
F
• Anterior and posterior diencephalic precursors (red) lie rostral to the
midbrain anlage (green)
bud stage
1 somite stage
F
F
F
F
Fezl + Barhl2
• The presumptive telencephalon (horse shoe in red) lies along the anterior
border of the neural plate. The medial red domain is the hypothalamus and
the anterior diencephalon is in green.
• Summary schematic of the fate of the neural plate territories.
Telencephalon (TEL)
Hypothalamus (Hypo)
Prethalamu (Pth)
Thalamus (Th)
TEL
Th
Pth
Pretectum
Epithalamus
Pineal gland
Basal diencephalon
Hypo.
Neurogenesis in 24hpf brain:
- Detection of post-mitotic neurons using anti-Hu (elav) antibody.
Dorsal view, rostral to the left.
Axonal tracts in 28 hpf CNS:
- Detection using anti-acetylated tubulin antibody.
Axonal tracts in 48hpf CNS:
Detection using anti-acetylated tubulin antibody. DAB revelation (secondary
IgG, HRP conjugate)
pc
soc
poc
CB: Cerebellum.; Hb: Habenula; OB: Olfactory Bulb; OE: Olfactory Epithelium; OT: Optic Tectum;
TEL: Telencephalon In red, acetylated tubulin staining and in green GFP in various transgenic lines.
Photo Gallery
- Axon detection using anti-acetylated tubulin antibody (secondary IgG, Alexa
Fluor conjugate). Confocal stack.
- Cranial nerves expressing GFP in the MAPT-GFP transgenic 3.5 day old
embryo.
- Dorsal view, anterior to the left, of a 3 day old dlx4/6GFP transgenic embryo
showing dlx4 and dlx6 expressing cells in green and axons in red (anti-tubulin
staining)
- Lateral views of the trunk
of a 30hpf MAPT-GFP
transgenic embryo.
GFP in green and
acetylated tubulin in red.
- Ventral views, anterior to the top of wild-type (A) and ace/fgf8-/- (B) 3 day old
embryos. Arrow indicates misrouted axons.