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Chapter 14: Animal Viruses How do animal viruses differ from bacterial viruses? • Attachment or entry into the cell • Replication of viral nucleic acid (remember eukaryotic cells have a nucleus) • Uncoating step is required by animal viruses • Exit the host cell by budding or shedding Entry by membrane fusion Entry by endocytosis Replication of nucleic acid and generation of mRNA Release by budding Acute viral infections • Usually short in duration • Host develops long lasting immunity • Infection of the virus results in a productive infection…host cells die as a result of infection General Steps of Acute Viral infection • • • • • • • Attachment Entry into host cell Targeting where it will reproduce Uncoating of the capsid Synthesis of proteins, replication of nucleic acid Maturation Cell lysis Can you identify some examples of viruses that produce an acute viral infection? • Are they naked viruses, or viruses with envelopes? Persistent infections • Virus is continually present in the body, released by budding • Three categories – Latent infections – Chronic infections – Slow infections Latent Infections • Persistent infection with symptomless period followed by reactivation of virus and symptoms • Example of latent viruses are found in the family Herpesviridae – Herpes simplex virus -1 – Herpes simplex virus -2 Latent Viral infections • All of these viruses are in the Herpesviridae family Herpesviridae Family • Double stranded DNA (dsDNA), enveloped virus -herpes simplex 1(cold sores) -herpes simplex 2 (genital herpes) -Varicella-zoster virus (chicken pox, shingles) -Epstein-Barr (infectious mono and Burkitt’s lymphoma) Herpes Simplex virus-1 HSV-1 reactivation Herpes simplex-1 • HSV-1 causes fever blisters, HSV-2 genital herpes • Symptoms: fluid filled skin lesions • Treatment: Acyclovir Varicella (chickenpox) and Herpes Zoster (Shingles) • HSV-3 causes chicken pox and latent activation known as shingles • Acquired by respiratory route, 2 weeks later see vesicles on skin • Vaccine established in 1995 for chickenpox Epstein Barr • Causes infectious mononucleosis • Acquire by saliva, incubation period is 4-7 weeks • Identify by -lobed lymphocytes -heterophile antibodies -fluorescent antibody tests Chronic infections • Infectious virus present at all times • Disease may be present or absent • Best example is Hepatitis Type B virus – Carriers produce virus detected in blood, saliva, and semen – Unique replication of dsDNA Chronic Viral infections Hepadnaviridae family • dsDNA virus, enveloped • Hepatitis B -passes through intermediate stage (RNA) -three particles in blood Dane filamentous sphericle -exposure through blood/body fluids Hepatitis B • Incubation period is ~12 weeks • 10% of cases become chronic, mortality rate is less than 1% • About 40% of the chronic cases die of liver cirrhosis Flaviviridae (+) ssRNA, enveloped • Hepatitis C virus – – – – Obtain from blood/body fluids Incubation period averages 6 weeks Hard to screen blood for the virus 85% of all cases become chronic Slow Infections • Infectious agent increases in amount over a long time during which there are no symptoms • Examples are HIV found in the Retroviridae family • Retroviruses use reverse transcriptase to replicate ssRNA Retrovirus Retroviridae-multiple strands of ()RNA • HIV -infects Helper T cells -requires the enzyme reverse transcriptase -integrates as a provirus -is released by budding, or lyses the cell Viruses and tumors • dsDNA viruses are most common to cause viral-induced tumors • Cancer is result of integration of viral genes into the host chromosome • Transforming genes are called oncogenes • Examples: papillomavirus, herpesvirus Viruses associated with cancers If multiple forms infect one cell…reassortment can occur Orthomyxoviridae-multiple strands of (-)RNA • Influenza virus – Consists of 8 segments of RNA – Envelope has H spikes (hemagglutinin) and N spikes (neuraminidase) – Incubation is 1-3 days – Symptoms include: chills, fever, headache, muscle aches, may lead to cold-like symptoms Influenza virus Ways to study viruses • Since viruses grow in living cells….need a live cell to culture them – Cell culture/tissue culture – Embryonated chicken eggs Cell Culture Quantitation of viruses: count plaques Proteinaceous infectious particles: PRIONS • 1982 Stanley Prusiner proposed that there were infectious proteins • Caused the disease “scrapie” in sheep • Caused the “mad-cow”disease in 1987 • Human forms suggest a genetic component Prions • Contain no nucleic acid • Abnormal protein promotes conformational change to normal protein • Results in damage to neurons…transmissible spongiform encephalopahthies Brain with spongiform encephalopathy Infections caused by prions Mechanism of prion replication Picornaviridae (+) ssRNA • Hepatitis A -obtain through fecal-oral route, enters GI tract and multiplies -incubation period is ~4 weeks -symptoms include: anorexia, malaise, nausea, diarrhea, abdominal discomfort, fever, and chills lasting 2-21 days Rhabdoviridae (-)ssRNA, enveloped • Rabies virus -enters the skin and multiplies in skeletal muscle and connective tissue -virus travels along nerves to the CNS causing encephalitis Pathology of rabies